Joao Augusto Oshiro Junior | University State of Paraiba - UEPB - Brazil (original) (raw)

Papers by Joao Augusto Oshiro Junior

The N-acylhydrazone subunit is present in several bioactive molecules and drug prototype candidat... more The N-acylhydrazone subunit is present in several bioactive molecules and drug prototype candidates, yet it remains under-explored. The association of an N-acylhydrazone derivative (JR19) with chitosan (CHI), a biopolymer with attractive pharmaceutical characteristics, may represent an innovative proposal for the treatment of topical inflammation. The aim of this work was to perform a physical-chemical characterization of chitosan films containing JR19 using differential scanning calorimetry (DSC), thermogravimetry and its derivative (TG/DTG), Fourier-transform infrared spectroscopy, and X-ray diffraction techniques. The films were developed using the sol-gel process and, according to the DSC curves, demonstrated the compatibility of the physical mixture (CHI + JR19) and the absence of chemical incompatibility of the polymer films incorporated with JR19. The TG/DTG presented similar characteristics of loss of mass between the samples, the prevailing characteristics of CHI. The infrared spectra of the physical mixture and the film with the molecule exhibited bands characteristic of the functional groups of JR19 in the same absorption region. The reflections observed in the diffractograms indicated a decrease in the crystallinity of the films when compared to the physical mixture and omission of some reflections of JR19 in the films. Therefore, the techniques used enabled the characterization of the formulation of CHI and JR19, proving very promising for the development of these films/dressings as an innovative proposal for pharmaceutical treatment, considering their low cost, biocompatibility, and the biological properties of JR19 against different diseases (parasitic, viral, bacterial, fungal, analgesic, anti-inflammatory, and antitumor).

Bioactive molecules such as peptides and proteins can optimize the repair of bone tissue; however... more Bioactive molecules such as peptides and proteins can optimize the repair of bone tissue; however, the results are often unpredictable when administered alone, owing to their short biological half-life and instability. Thus, the development of bioactive molecule-loaded drug delivery systems (DDS) to repair bone tissue has been the subject of intense research. DDS can optimize the repair of bone tissue owing to their physicochemical properties, which improve cellular interactions and enable the incorporation and prolonged release of bioactive molecules. These characteristics are fundamental to favor bone tissue homeostasis, since the biological activity of these factors depends on how accessible they are to the cell. Considering the importance of these DDS, this review aims to present relevant information on DDS when loaded with osteogenic growth peptide and bone morphogenetic protein. These are bioactive molecules that are capable of modulating the differentiation and proliferation of mesen-chymal cells in bone tissue cells. Moreover, we will present different approaches using these peptide and protein-loaded DDS, such as synthetic membranes and scaffolds for bone regeneration, synthetic grafts, bone cements, liposomes, and micelles, which aim at improving the therapeutic effectiveness, and we will compare their advantages with commercial systems.

Pharmaceutical design has enabled important advances in the prevention, treatment, and diagnosis ... more Pharmaceutical design has enabled important advances in the prevention, treatment, and diagnosis of diseases. The use of nanotechnology to optimize the delivery of drugs and diagnostic molecules is increasingly receiving attention due to the enhanced efficiency provided by these systems. Understanding the structures of nanocarriers is crucial in elucidating their physical and chemical properties, which greatly influence their behavior in the body at both the molecular and systemic levels. This review was conducted to describe the principles and characteristics of techniques commonly used to elucidate the structures of nanocarriers, with consideration of their size, morphology, surface charge, porosity, crystalline arrangement, and phase. These techniques include X-ray diffraction, small-angle X-ray scattering, dynamic light scattering, zeta potential, polarized light microscopy, transmission electron microscopy, scanning electron microcopy, and porosimetry. Moreover, we describe some of the commonly used nanocarriers (liquid crystals, metal–organic frameworks, silica nanospheres, liposomes, solid lipid nanoparticles, and micelles) and the main aspects of their structures

Stimuli-responsive drug-delivery nanocarriers (DDNs) have been increasingly reported in the liter... more Stimuli-responsive drug-delivery nanocarriers (DDNs) have been increasingly reported in the literature as an alternative for breast cancer therapy. Stimuli-responsive DDNs are developed with materials that present a drastic change in response to intrinsic/chemical stimuli (pH, redox and enzyme) and extrinsic/physical stimuli (ultrasound, light, magnetic field and electric current). In addition, they can be developed using different strategies, such as functionalization with signaling molecules, leading to several advantages, such as (a) improved pharmaceutical properties of liposoluble drugs, (b) selectivity with the tumor tissue decreasing systemic toxic effects, (c) controlled release upon different stimuli, which are all fundamental to improving the therapeutic effectiveness of breast cancer treatment. Therefore, this review summarizes the use of stimuli-responsive DDNs in the treatment of breast cancer. We have divided the discussions into intrinsic and extrinsic stimuli and have separately detailed them regarding their definitions and applications. Finally, we aim to address the ability of these stimuli-responsive DDNs to control the drug release in vitro and the influence on breast cancer therapy, evaluated in vivo in breast cancer models.

Background: Plant extracts loaded in nanostructured drug delivery systems (NDDSs) have been repor... more Background: Plant extracts loaded in nanostructured drug delivery systems (NDDSs) have been reported as an alternative to current therapies for treating parasitic and antimicrobial diseases. Among their advantages , plant extracts in NDSSs increase the stability of the drugs against environmental factors by promoting protection against oxygen, humidity, and light, among other factors; improve the solubility of hydrophobic compounds ; enhance the low absorption of the active components of the extracts (i.e., biopharmaceutical classification II), which results in greater bioavailability; and control the release rate of the substances, which is fundamental to improving the therapeutic effectiveness. In this review, we present the most recent data on NDDSs using plant extracts and report results obtained from studies related to in vitro and in vivo biological activities.

RESUMO A vitamina C é um ativo interessante para incorporação em formulações cosméticas devido às... more RESUMO A vitamina C é um ativo interessante para incorporação em formulações cosméticas devido às suas propriedades benéficas para a pele. Porém, sua instabilidade pode limitar seu uso. Desta forma, esta pesquisa teve como objetivo avaliar a estabilidade da vitamina C em diferentes tipos de embalagens, submetendo-as a diferentes condições de armazenamento. Uma formulação emulsionada contendo vitamina C foi preparada e submetida ao estudo de estabilidade em embalagem de plástico, vidro e alumínio. Os caracteres organolépticos, físico-químicos e o teor do ativo foram acompanhados. Através dos resultados foi possível concluir que a utilização da bisnaga de alumínio é mais vantajosa, já que, provavelmente possui maior impermeabilidade e pode agir como barreira contra a luz, mantendo desta forma inalterados, por um período maior do que as outras embalagens, os caracteres iniciais da formulação. Palavras-chave: Vitamina C; Embalagem; Estabilidade. ABSTRACT Vitamin C is an interesting active substance for incorporation into cosmetic formulations due to its beneficial properties to the skin. But its instability may limit its use. Thus, this research aimed at evaluating the stability of vitamin C in various types of packaging, subjecting them to different storage conditions. An emulsified formulation containing vitamin C was prepared and subjected to stability studies in plastics, glass and aluminum packaging. The organoleptic, physical-chemical characteristics and active substance content were followed. The results allowed us to conclude that the use of aluminum tube is more advantageous since it is likely to have greater imperviousness and can act as a barrier against light, thus maintaining the initial characters of the formulation unchanged for a longer period than other packages.

Resumo O controle da contaminação microbiana de fórmulas magistrais, entre elas cosméticos, produ... more Resumo O controle da contaminação microbiana de fórmulas magistrais, entre elas cosméticos, produtos de higiene e medicamentos, é de fundamental importância, estando relacionado com a Saúde Pública, a qualidade e a estabilidade do produto. Devido ao grande número de micro-organismos que podem estar presentes nas formulações magistrais, a contaminação deve ser evitada ao longo do processo de fabricação. Considerando a importância deste tema, este trabalho teve como objetivo a realização de uma revisão da literatura visando esclarecer pontos de importância relacionados à contaminação microbiológica de produtos magistrais. Foram utilizados artigos, dissertações e teses, além de publicações da Agência Nacional de Vigilância Sanitária datados de 2000 a 2017. Foi verificado que as fontes de contaminação são pontos críticos na fabricação dos produtos e devem ser monitorados de acordo com as Boas Práticas de Fabricação. Existe grande preocupação com a manutenção da qualidade dos produtos não estéreis, visto que o desvio de qualidade pode acarretar em grande prejuízo ao usuário incluindo o desenvolvimento de patologias severas, dependendo do estado de saúde do mesmo. A análise da formulação é realizada buscando micro-organismos que são patológicos ao homem, como Escherichia coli, Salmonella spp., Staphylococcus aureus e Pseudomonas aeruginosa. A manutenção da qualidade em produtos farmacêuticos é um grande desafio, pois, embora existam vários métodos e se tenha um vasto conhecimento na área da Microbiologia, invariavelmente surgem problemas relacionados à contaminação. A orientação teórica e prática detalhada dos métodos qualitativos e quantitativos é de absoluta necessidade para que os eventuais desvios de qualidade sejam solucionados rapidamente. Palavras-chave: Contaminação Microbiológica; Farmácia Magistral; Conservantes. Microbiological contamination of magister products Abstract The control of microbial contamination of magistral formulations, including cosmetics, toiletries and medicines, is of fundamental importance and related to Public Health, quality and stability of the products. Due to the large number of microorganisms that may be present in magistral formulations, contamination should be avoided during the manufacturing process. Considering the importance of this subject, the aim of this study was to prepare of a literature review aiming to clarify important statements related to the microbial contamination of magistral products. Research papers, dissertations and theses were used, as well as publications of the Agência Nacional de Vigilância Sanitária dated from 2000 to 2017. It was verified that sources of contamination are critical points in the manufacture of the products and should be monitored according to the Good Manufacturing Practices. There is a great concern with maintaining the quality of non-sterile products, since the deviation of quality can cause great harm to the user including the development of severe pathologies, depending on the state of health of the same. Analysis of the formulation is performed by looking for microorganisms that are pathological to humans, such as Escherichia coli, Salmonella spp., Staphylococcus aureus and Pseudomonas aeruginosa. The maintenance

The vaginal mucosa is a very promising route for drug administration due to its high permeability... more The vaginal mucosa is a very promising route for drug administration due to its high permeability and the possibility to bypass first pass metabolism; however, current vaginal dosage forms present low retention times due to their dilution in vaginal fluids, which hampers the efficacy of many pharmacological treatments. In order to overcome these problems, this study proposes to develop a mucoadhesive in situ gelling liquid crystalline precursor system composed of 30% of oleic acid and cholesterol (7:1), 40% of ethoxylated and propoxylated cetyl alcohol, and 30% of a dispersion of 16% Poloxamer 407. The effect of the dilution with simulated vaginal fluid (SVF) on this system was evaluated by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheological studies, texture profile analysis (TPA), mucoadhesion study, in vitro drug release test using hypericin (HYP) as drug model, and cytotoxicity assay. PLM and SAXS confirmed the formation of an isotropic system. After the addition of three different concentrations of SVF (30, 50, and 100%), the resultant formulations presented anisotropy and characteristics of viscous lamellar phases. Rheology shows that formulations with SVF behaved as a non-Newtonian fluid with suitable shear thinning for vaginal application. TPA and mucoadhesion assays indicated the formation of long-range ordered systems as the amount of SVF increases which may assist in the fixation of the formulation on the vaginal mucosa. The formulations were able to control about 75% of the released HYP demonstrating a sustained release profile. Finally, all formulations acted as safe vaginal drug delivery systems.

Dyeing processes are highly important for differentiation of textile products, and in general, th... more Dyeing processes are highly important for differentiation of textile products, and in general, they use a huge amount of water and chemical additives, generating a great environmental impact. In the last years, the interest in finding alternative environmentally friendly solvents for these processes has increased, with a double aim: to decrease the quantity of water used and to improve the dyeing quality. In this work, we analyze an alternative procedure to dye cotton fabrics with only two dyeing agents, a polyfunctional reactive dye and a protic ionic liquid (PIL) as an alternative solvent, avoiding the need for common chemical additives into the conventional process, thus reducing the amount and concentration of pollutants. The proposed procedure allows PIL recycling with no appreciable loss of efficiency, since after dyeing, the unfixed dye remains active in the bath, following the known exhaustion dyeing method. A collection of 13 PILs were studied with the aim of analyzing their effectiveness as dyeing solvents in terms of different standard dyeing quality parameters such as absorption color, tensile strength, and surface morphology of the cotton dyed. The results obtained using PIL as dyeing solvents in the absence of any auxiliary agent showed an outstanding effect when compared with the aqueous process under the same operational conditions.

The aim of this study was to develop a new barrier membrane from ureasil-polyether hybrid materia... more The aim of this study was to develop a new barrier membrane from ureasil-polyether hybrid materials for guided bone regeneration. The membrane was prepared via the sol-gel route by using polyethylene oxide and polypropylene oxide polymer blends; dexamethasone was used as a drug model for in vitro release testing. Membrane swelling was monitored by small-angle x-ray scattering (SAXS) measurement and bone formation was investigated by microtomography. In vitro drug release testing revealed sustained profiles that could be controlled by varying the proportions of the polymers. SAXS analysis showed that the polymer blend can control the high swelling observed when using ureasil-polyethylene oxide material. Histological results revealed that the ureasil-polyether blend promoted local inflammation similar to the response caused by commercial collagen membrane. The results of microtomography showed that the osteo-regeneration of bone defects by using ureasil-polyether membranes was similar to the effect promoted by commercial collagen membrane. These results indicate that ureasil-polyether membranes are promising candidates for barrier membrane, considering their low cost, luminescence, control of drug release, ease of handling, and biocompatibility.

Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis. Cessation of treatment befo... more Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis. Cessation of treatment before the recommended conclusion may lead to the emergence of multidrug-resistant strains. The aim of this study was to develop nanostructured lipid carriers (NLCs) for use in the treatment of M. tuberculosis. The NLCs comprised the following lipid phase: 2.07% polyoxyethylene 40 stearate, 2.05% caprylic/capric triglyceride, and 0.88% polyoxyl 40 hydrogenated castor oil; the following aqueous phase: 3.50% poloxamer 407 (F1–F6), and 0.50% cetyltrimethylammonium bromide (F7–F12); and incorporated the copper(II) complexes [CuCl2(INH)2]·H2O (1), [Cu(NCS)2(INH)2]·5H2O (2), and [Cu(NCO)2(INH)2]·4H2O (3) to form compounds F11.1, F11.2, and F11.3, respectively. The mean diameter of F11, F11.1, F11.2, and F11.3 ranged from 111.27±21.86 to 134.25±22.72 nm, 90.27±12.97 to 116.46±9.17 nm, 112.4±10.22 to 149.3±15.82 nm, and 78.65±6.00 to 122.00±8.70 nm, respectively. The polydispersity index values for the NLCs ranged from 0.13±0.01 to 0.30±0.09. The NLCs showed significant changes in zeta potential, except for F11.2, with F11, F11.1, F11.2, and F11.3 ranging from 18.87±4.04 to 23.25±1.13 mV, 17.03±1.77 to 21.42±1.87 mV, 20.51±1.88 to 22.60±3.44 mV, and 17.80±1.96 to 25.25±7.78 mV, respectively. Atomic force microscopy confirmed the formation of nanoscale spherical particle dispersions by the NLCs. Differential scanning calorimetry determined the melting points of the constituents of the NLCs. The in vitro activity of copper(II) complex-loaded NLCs against M. tuberculosis H37Rv showed an improvement in the anti-TB activity of 55.4, 27.1, and 41.1 times the activity for complexes 1, 2, and 3, respectively. An in vivo acute toxicity study of complex-loaded NLCs demonstrated their reduced toxicity. The results suggest that NLCs may be a powerful tool to optimize the activity of copper(II) complexes against M. tuberculosis.

Based on previous studies, it has been found that goji berry (GB), popularly known as a 'miracle ... more Based on previous studies, it has been found that goji berry (GB), popularly known as a 'miracle fruit', has excellent antioxidant potential and can be used in the treatment of skin disorders associated with ageing. This study aimed to incorporate GB into a structured cosmetic in order to optimise its penetration. Stability studies of the formulation, determination of the antioxidant activity of the extract and of the formulation, rheological measurements, SAXS, polarised light microscopy and bioadhesion analyses were performed. The results indicated the antioxidant activity of the extract, which can be incorporated into an emulsified cosmetic formulation. The emulsified formulation containing the extract remained stable, even after being submitted to thermal and luminous stresses for 30 days. In addition, rheological tests revealed that the addition of the GB soft extract reduced the viscosity of the formulation, increasing thixotropy and deformation. These systems were characterised by SAXS as a lamellar phase, which was confirmed by polarised light microscopy. These highly organised structures indicate their excellent stability. In vitro bioadhesion experiments revealed that these formulations exhibited skin adhesion strength statistically similar to commercial anti-ageing formulation. These results suggest that this formulation has excellent potential to be used as a topical treatment for ageing.

The hydrolysis and condensation reactions involved in synthesis of ureasil-polyether films influe... more The hydrolysis and condensation reactions involved in synthesis of ureasil-polyether films influence the film formation time and the number of chemical groups able to form hydrogen bonds, responsible for the bioadhesion, with the biological substrate. The objective of this work was to study the influence of the use of an acid catalyst (hydrochloric acid) and a basic catalyst (ammonium fluoride) in the hydrolysis and condensation reactions on the time formation and bioadhesion of ureasil-polyether films. The toxicity of the films was evaluated. The MTT assay has shown cell viability of human skin keratinocytes higher than 70% of all analyzed materials suggesting low cytotoxicity. The bioadhesion of the films is strongly dependent on the viscosity and hydrophilic/hydrophobic balance of the polyether chains used to synthetize the hybrid molecules. The use of acid catalyst promotes the formation of less viscous films with higher bioadhesion. The hybrids formed by more hydrophilic PEO chains are more bioadherent, since they can interact more efficiently with the water present in the stratum corneum increasing the bioadhesion. Due to their low toxicity and high bioadhesion, the ureasil-PEO films obtained by using HCl as catalyst agent are good candidates for application to the skin as bioadhesive films.

This is a review of hybrid materials based on silica as an inorganic phase used as drug delivery ... more This is a review of hybrid materials based on silica as an inorganic phase used as drug delivery systems (DDS). Silica based DDS have shown effectivity when compared with traditional delivery systems. They present advantages such as: (a) ability to maintain the therapeutic range with minor variations; (b) prevention of local and systemic toxic effects; (c) plasma concentrations increase of substances with a short half-life; and (d) reduction of the number of daily doses, which may increase patient adherence to the treatment. These advantages occur due to the physical, chemical and optical properties of these materials. Therefore, we discuss the properties and characteristics of them and we present some applications, using different approaches of DDS to ensure therapeutic effectiveness and side effects reduction such as implantable biomaterial, film-forming materials, stimuli-responsive systems and others.

Camellia sinensis, which is obtained from green tea extract (GTE), has been widely used in therap... more Camellia sinensis, which is obtained from green tea extract (GTE), has been widely used in therapy owing to the antioxidant, chemoprotective, and anti-inflammatory activities of its chemical components. However, GTE is an unstable compound, and may undergo reactions that lead to a reduction or loss of its effectiveness and even its degradation. Hence, an attractive approach to overcome this problem to protect the GTE is its incorporation into liquid crystalline systems (LCS) that are drug delivery nanostructured systems with different rheological properties, since LCS have both fluid liquid and crystalline solid properties. Therefore, the aim of this study was to develop and characterize GTE-loaded LCS composed of polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol, avocado oil, and water (F25E, F29E, and F32E) with different rheological properties and to determine their anti-inflammatory efficacy. Polarized light microscopy revealed that the formulations F25, F29, and F32 showed hexagonal, cubic, and lamellar liquid crystalline mesophases, respectively. Rheological studies showed that F32 is a viscous Newtonian liquid, while F25 and F29 are dilatant and pseudoplastic non-Newtonian fluids, respectively. All GTE-loaded LCS behaved as pseudoplastic with thixotropy; furthermore, the presence of GTE increased the S values and decreased the n values, especially in F29, indicating that this LCS has the most organized structure. Mechanical and bioadhesive properties of GTE-unloaded and-loaded LCS corroborated the rheological data, showing that F29 had the highest mechanical and bioadhesive values. Finally, in vivo inflammation assay revealed that the less elastic and consistent LCS, F25E and F32E presented statistically the same anti-inflammatory activity compared to the positive control, decreasing significantly the paw edema after 4 h; whereas, the most structured and elastic LCS, F29E, strongly limited the potential effects of GTE. Thereby, the development of drug delivery systems with suitable rheological properties may enhance GTE bioavailability, enabling its administration via the skin for the treatment of inflammation.

The use of biocompatible polymers such as Hydroxypropylmethylcellulose (HPMC), Hydroxyethylcellul... more The use of biocompatible polymers such as Hydroxypropylmethylcellulose (HPMC), Hydroxyethylcellulose (HEC), Carboxymethylcellulose (CMC), and Carbopol in solid formulations results in mucoadhesive systems capable of promoting the prolonged and localized release of Active Pharmaceutical Ingredients (APIs). This strategy represents a technological innovation that can be applied to improving the treatment of oral infections, such as oral candidiasis. Therefore, the aim of this study was to develop a tablet of Ximenia americana L. from mucoadhesive polymers for use in the treatment of oral candidiasis. An X. americana extract (MIC of 125 µg·mL −1) was obtained by turbolysis at 50% of ethanol, a level that demonstrated activity against Candida albicans. Differential Thermal Analysis and Fourier Transform Infrared Spectroscopy techniques allowed the choice of HPMC as a mucoadhesive agent, besides polyvinylpyrrolidone, magnesium stearate, and mannitol to integrate the formulation of X. americana. These excipients were granulated with an ethanolic solution 70% v/v at PVP 5%, and a mucoadhesive tablet was obtained by compression. Finally, mucoadhesive strength was evaluated, and the results demonstrated good mucoadhesive forces in mucin disk and pig buccal mucosa. Therefore, the study allowed a new alternative to be developed for the treatment of buccal candidiasis, one which overcomes the inconveniences of common treatments, costs little, and facilitates patients' adhesion.

Polymers, 2019

Cellulose is among the top 5 excipients used in the pharmaceutical industry. It has been consider... more Cellulose is among the top 5 excipients used in the pharmaceutical industry. It has been considered one of the main diluents used in conventional and modern dosage forms. Therefore, different raw materials of plant origin have been evaluated as potential alternative sources of cellulose. In this context, Opuntia ficus-indica L. Miller (palma forrageira), a plant of the cactus family that has physiological mechanisms that provide greater productivity with reduced water requirements, is an interesting and unexplored alternative for extracting cellulose. By using this source, we aim to decrease the extraction stages and increase the yields, which might result in a decreased cost for the industry and consequently for the consumer. The aim of this work was to investigate the use of Opuntia ficus-indica L. Miller as a new source for cellulose extraction, therefore providing an efficient, straight forward and low-cost method of cellulose II production. The extraction method is based on the oxidation of the lignins. The obtained cellulose was identified and characterized by spectroscopic methods (FTIR and NMR), X-ray diffraction, thermal analysis (TGA-DTG and DSC) and scanning electron microscopy. The results confirmed the identity of cellulose and its fibrous nature, which are promising characteristics for its use in the industry and a reasonable substrate for chemical modifications for the synthesis of cellulose II derivatives with different physicochemical properties that might be used in the production of drug delivery systems and biomaterials.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, 2016

The biocompatibility of ureasil-polyether hybrid materials has been tested for future application... more The biocompatibility of ureasil-polyether hybrid materials has been tested for future application as
membrane barrier. The authors evaluated ureasil-polyether hybrids membranes with different swollen
behaviors: more swellable ureasil-poliethylene oxide (ureasil-PEO) of molecular wheigth 1900 g.mol-1
and less swellable ureasil-polypropilene oxide (ureasil-PPO) of molecular wheigth 400 g.mol-1. The
swollen behavior was monitored by SAXS measurements and in vivo assays using Rattus Norvegicus were
used to study their biocompatibility. The results obtained were compared with the same treatment made
with collagen commercial membranes. It was observed that for commercial collagen membranes,
inflammatory levels declined after seven days. The ureasil-PEO induced a greater influx of inflammatory
cells during 30 days which could be associated with the higher degree of swelling. The ureasil-PPO
membranes exhibited a smaller level of inflammatory cells and are good candidates for application as
biomaterial, considering their low cost, ability to deliver active molecules, and biocompatibility.

Polymers, 2019

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, 2016