Alexandre R Bello | UERJ - Universidade do Estado do Rio de Janeiro / Rio de Janeiro State University (original) (raw)

Alexandre R Bello

Graduated in Biological Sciences from the State University of Rio de Janeiro (1986), Master in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1991). Visiting researcher at Department. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, USA 1992-1994. Doctor of Science (Ph.D) from the Federal University of Rio de Janeiro (1996). Postdoctoral Fellow at the Interdisciplinary Medical Research Laboratory / IOC / Fiocruz (2015). Associate Professor at the State University of Rio de Janeiro (UERJ), in the Department of Microbiology, Immunology and Parasitology (DMIP). Chairman of the Internal Biosafety Committee of the Faculty of Medical Sciences (CIBio-FCM / UERJ). Experience in Molecular Biology, Molecular Genetics of Microorganisms, Molecular Parasitology acting on the following topics:. Molecular diagnostics, molecular characterization, PCR, Leishmania, AP endonucleases, Cryptosporidium spp, Escherichia coli, multidrug resistance mechanisms in enterobacteria and Laboratory Biosafety.

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Papers by Alexandre R Bello

Memórias do Instituto Oswaldo Cruz, 2003

Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura ... more Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing. T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.

Memórias do Instituto Oswaldo Cruz, 2004

Despite toxoplasmosis being a common infection among human and other warm-blooded animals worldwi... more Despite toxoplasmosis being a common infection among human and other warm-blooded animals worldwide, there are no findings about Toxoplasma gondii evolutionary forms in ancient populations. The molecular techniques used for amplification of genetic material have allowed recovery of ancient DNA (aDNA) from parasites contained in mummified tissues. The application of polymerase chain reaction (PCR) to paleoparasitological toxoplasmosis research becomes a promising option, since it might allow diagnosis, acquisition of paleoepidemiological data, access to toxoplasmosis information related origin, evolution, and distribution among the ancient populations. Furthermore, it makes possible the analysis of parasite aDNA aiming at phylogenetic studies. To standardize and evaluate PCR applicability to toxoplasmosis paleodiagnostic, an experimental mummification protocol was tested using desiccated tissues from mice infected with the ME49 strain cysts, the chronic infection group (CIG), or infe...

The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

There is a high incidence of infections caused by betalactamase-producing Gram-negative microorga... more There is a high incidence of infections caused by betalactamase-producing Gram-negative microorganisms in Brazil. These organisms are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. The present study was conducted in sentinel rectal swabs from patients admitted to a cardiac surgery hospital in Rio de Janeiro, from January through December 2007, in a consecutive manner. The aim of the study was to characterize the genotype and phenotype of these isolates from colonized patients. Biochemical tests, antimicrobial susceptibility tests, a confirmatory test for the expression of extended spectrum betalactamase (ESBL) production and polymerase chain reaction for the blaTEM, blaSHV, CTX-M1, Toho-1 and AmpC genes were performed at the University Hospital of Universidade do Estado do Rio de Janeiro (UERJ). The most frequently isolated bacteria were Escherichia coli 9/41 (21.95%) and Klebsiella pneumoniae 14/41 (34.1%). In 24/41 (58%)...