Magui Vallim | UERJ - Universidade do Estado do Rio de Janeiro / Rio de Janeiro State University (original) (raw)
Papers by Magui Vallim
Experiências em ensino de ciências, 2017
Relatamos as experiências ao se realizar o Ensino de Genética através do material didático Linhag... more Relatamos as experiências ao se realizar o Ensino de Genética através do material didático Linhagem de DNA mitocondrialbaseado na obra O tempo e o vento, de Érico Veríssimo. Este foi aplicado de forma interativa, em um contexto interdisciplinar, em turmas do segundo e terceiro ano do Ensino Médio em dois colégios públicos do Estado do Rio de Janeiro. No colégio de ensino tradicional, o romance foi inicialmente abordado na aula de Português e, em seguida, o material didático foi aplicado na aula de Biologia. Em outro colégio, cujo ensino é realizado de forma interdisciplinar, os professores de Português, História e Biologia trabalharam, simultaneamente, em torno do tema do material didático. A proposta de trabalho interdisciplinar foi bem recebida nos dois colégios e gerou bons resultados, embora a atividade tenha sido aplicada de forma plena, em uma experiência mais enriquecedora, no colégio de ensino não convencional. Palavras-chave: Interdisciplinaridade; Ensino de Ciências; Ensino de Genética; O tempo e o vento; Érico Veríssimo.
Genética na Escola, May 4, 2019
Journal of Medicinal Plants Research, 2010
They describe in this paper that the dolastane diterpenes 4-hydroxy-9,14-dihydroxydolasta-1(15),7... more They describe in this paper that the dolastane diterpenes 4-hydroxy-9,14-dihydroxydolasta-1(15),7-diene (1) and 4,7,14-trihydroxydolasta-1(15),8-diene (2), isolated from the marine brown alga Canistrocarpus cervicornis inhibited HSV-1 infection in Vero cells. The concentration required to inhibit HSV-1 replication was not cytotoxic. Their results suggest that the structures of diterpenes 1 and 2, dolastane diterpenes from Brazilian brown algae, are promising for future antiviral drugs. Key words: Canistrocarpus cervicornis, dictyotaceae, phaeophyceae, dolastane diterpene, cytotoxicity, antiviral against HSV-1.
Revista Brasileira de Farmacognosia, 2011
The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linea... more The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linearol/isolinearol) that were isolated from the marine brown alga Canistrocarpus cervicornis were evaluated against biological activities of Lachesis muta snake venom. In vitro assays showed that the crude extract and the diterpenes were able to inhibit the clotting and proteolytic activity induced by L. muta crude venom, but not the hemolytic activity. However, only the diterpenes inhibited the hemolysis caused by a purified phospholipase A 2 previously isolated from L. muta venom, denoted LM-PLA 2-I. Interestingly, the crude algal extract and the diterpenes were able to protect mice from hemorrhage induced by L. muta venom. Thus, we may conclude that marine algae are rich and powerful sources of molecules that may be used against L. muta accidents in order to improve treatment of envenomation by this snake.
Revista Ibero-Americana de Estudos em Educação
A contemporaneidade se caracteriza pela celeridade e pela falta de tempo para ler; tal peculiarid... more A contemporaneidade se caracteriza pela celeridade e pela falta de tempo para ler; tal peculiaridade motiva uma escassa dedicação aos textos clássicos, os quais são reconhecidamente capazes de promover uma experiência humanizadora. Neste estudo, buscamos avaliar a utilização da obra Cem Anos de Solidão em um projeto escolar interdisciplinar no qual a literatura assumiu a posição de eixo integrador das áreas de conhecimento. O projeto foi proposto aos docentes de uma escola pública do estado do Rio de Janeiro e adaptado para aplicação. A intervenção foi avaliada através da análise de conteúdo das redações produzidas pelos alunos e de questionários aplicados aos docentes. O papel integrador do projeto proporcionou ao aluno/leitor o desprendimento da realidade imediata, o exercício da imaginação, ampliação da compreensão e de visão de mundo. A intervenção foi positivamente avaliada pelos alunos e professores e o estímulo à leitura de outros clássicos foi fomentando.
Ciência & Educação (Bauru)
Resumo: Discutimos o impacto da utilização da ficção científica, associada ao enfoque Ciência, Te... more Resumo: Discutimos o impacto da utilização da ficção científica, associada ao enfoque Ciência, Tecnologia e Sociedade (CTS), no desenvolvimento crítico dos alunos e o consequente despertar para o exercício da cidadania. Para tal, aplicamos uma sequência didática para alunos do ensino Médio, baseada na obra Admirável mundo novo, em uma perspectiva Freireana. A atividade é fundamentada na discussão, a partir de material de apoio preparado para os temas CTS destacados da obra: consumismo, uso de drogas, manipulação genética e exclusão social. A intervenção foi avaliada por meio de questionário. Verificamos o desenvolvimento do senso crítico dos alunos através das relações das temáticas CTS da sociedade contemporânea com a sociedade criada por Aldous Huxley em 1932. Os alunos perceberam a atividade como interdisciplinar, relacionando os temas abordados com várias disciplinas, e valorizaram a prática pedagógica alternativa. Os estudantes também reconheceram que as temáticas expostas e de...
Revista Aproximando, 2015
ABSTRACT Marine organisms are rich sources of natural products that, among other activities, help... more ABSTRACT Marine organisms are rich sources of natural products that, among other activities, help to maintain the species equilibrium. Samples of the marine brown alga Canistrocarpus cervicornis (Kutzing) De Paula & De Clerck were collected in Buzios, Rio de Janeiro, Brazil, in June 2006. The extract was obtained in CH2Cl2 and subjected to fractionation by chromatographic methods in order to isolate and purify the compound (4R, 7R, 14S)-4 alpha, 7 alpha-diacethoxy-14-hydroxydolastane-1(15), 8-dien. Then, the effects of the extract and the dolastane diterpene on zygotes and gametes of the sea urchin Lytechinus variegatus were evaluated. The exposure of male and female gametes to the C. cervicornis extract promoted, respectively, a reduction of 10-30% in fertilization and a 20 to 70% decrease in the number of eggs. Furthermore, the exposure of zygotes to the extract inhibited their development up to 86.7 +/- 1.6% (at a concentration of 250 mu g mL(-1)), as well as generating abnormalities in 39-50% of zygotes. The results of the dolastane diterpene showed no evidence of inhibition in the zygotes' development, thought it was proved to induce anomalies. At higher concentrations (25 and 50 mu g mL(-1)), it was possible to observe cell lyses.
Molecules, 2015
Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wid... more Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wide range of toxic effects. Envenomation by Bothrops jararaca venom results in hemorrhage, edema, pain, tissue necrosis and hemolysis. In this work, the effect of a mixture of two secodolastane diterpenes (linearol/isolinearol), previously isolated from the Brazilian marine brown alga, Canistrocarpus cervicornis, was evaluated against some of the toxic effects induced by B. jararaca venom. The mixture of diterpenes was dissolved in dimethylsulfoxide and incubated with venom for 30 min at room temperature, and then several in vivo (hemorrhage, edema and lethality) and in vitro (hemolysis, plasma clotting and proteolysis) assays were performed. The diterpenes inhibited hemolysis, proteolysis and hemorrhage, but failed to inhibit clotting and edema induced by B. jararaca venom. Moreover, diterpenes partially protected mice from lethality caused by B. jararaca venom. The search for natural inhibitors of B. jararaca venom in C. cervicornis algae is a relevant subject, since seaweeds are a rich and powerful source of active molecules which are as yet but poorly explored. Our results suggest that
Revista Brasileira de Farmacognosia, 2011
The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linea... more The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linearol/isolinearol) that were isolated from the marine brown alga Canistrocarpus cervicornis were evaluated against biological activities of Lachesis muta snake venom. In vitro assays showed that the crude extract and the diterpenes were able to inhibit the clotting and proteolytic activity induced by L. muta crude venom, but not the hemolytic activity. However, only the diterpenes inhibited the hemolysis caused by a purified phospholipase A 2 previously isolated from L. muta venom, denoted LM-PLA 2-I. Interestingly, the crude algal extract and the diterpenes were able to protect mice from hemorrhage induced by L. muta venom. Thus, we may conclude that marine algae are rich and powerful sources of molecules that may be used against L. muta accidents in order to improve treatment of envenomation by this snake.
Brazilian Journal of Oceanography, 2007
Crude extracts of the brown seaweed Dictyota mertensii (Martius) Kützing collected at two distant... more Crude extracts of the brown seaweed Dictyota mertensii (Martius) Kützing collected at two distant and different places on the Brazilian coast, Búzios (Rio de Janeiro) and Fernando de Noronha (PE), were evaluated for defensive chemistry against the crab Pachygrapsus transversus, and the sea urchin Lytechinus variegatus. The extract from Búzios specimens of D. mertensii significantly inhibited the consumption by both P. transversus and L. varigetaus. Fractionation of the extracts of specimens of D. mertensii from Búzios and F. de Noronha followed by complementary assays revealed one active fraction from each location, which contained distinct defensive secondary metabolites. In each active fractions prenylated guaiane diterpenes were the major compounds. Dictyol H and epoxypachydictyol A were the most abundant compounds in Búzios and F. de Noronha, respectively, followed by minor components. Our results show a differential production of secondary metabolites in the two distant and dif...
Biochemical Systematics and Ecology, 2005
... diets and particular food chains, indicating specific herbivorous–algae relationships ( [Kele... more ... diets and particular food chains, indicating specific herbivorous–algae relationships ( [Kelecom and Teixeira, 1986] and [Pereira and Teixeira, 1999 ... The “Dictyota” group would include the genera Dictyota Lamouroux, Dilophus J. Agardh, Glossophora J. Agardh, Pachydictyon J ...
Revista Brasileira de Farmacognosia, 2011
Dictyotaceae are a rich source of secondary metabolites, especially terpenes. These natural produ... more Dictyotaceae are a rich source of secondary metabolites, especially terpenes. These natural products have been studied for their bioactivity for human and for their ecological role in nature. The present work highlights de diversity of the Dictyotaceae terpenes, emphasizing their bioactivities and the biogeography of their sources. The sesquiterpenes are found in Padina, Dictyopteris and Taonia. Although Dictyopteris and Padina can be found in all oceans, Taonia has a more restricted distribution. Diterpenes of mixed origin have been reported in Lobophora, Taonia and Stypopodium. Stypopodium is a typically tropical and warm temperate genus. Diterpenes with the spatane and seco-spatanes skeletons are known from Spatoglossum, Stoechospermum and Rugulopteryx. Spatoglossum is distributed over all the tropical marine world. Stoechospermum is known to occur in the Indian Ocean and Rugulopteryx is distributed mainly in Asia and on the Pacific Coast of Australia. The genus Dictyota contains diterpenes as the major metabolite and are present in all oceans, reaching even the cold temperate regions of northern and southern hemispheres. The terpenoids from Dictyotaceae exhibit bioactivities such as inhibition of herbivores and antifungal, cytotoxic, antibiotic, anti-inflammatory, insecticidal and antiviral activities.
Experiências em ensino de ciências, 2017
Relatamos as experiências ao se realizar o Ensino de Genética através do material didático Linhag... more Relatamos as experiências ao se realizar o Ensino de Genética através do material didático Linhagem de DNA mitocondrialbaseado na obra O tempo e o vento, de Érico Veríssimo. Este foi aplicado de forma interativa, em um contexto interdisciplinar, em turmas do segundo e terceiro ano do Ensino Médio em dois colégios públicos do Estado do Rio de Janeiro. No colégio de ensino tradicional, o romance foi inicialmente abordado na aula de Português e, em seguida, o material didático foi aplicado na aula de Biologia. Em outro colégio, cujo ensino é realizado de forma interdisciplinar, os professores de Português, História e Biologia trabalharam, simultaneamente, em torno do tema do material didático. A proposta de trabalho interdisciplinar foi bem recebida nos dois colégios e gerou bons resultados, embora a atividade tenha sido aplicada de forma plena, em uma experiência mais enriquecedora, no colégio de ensino não convencional. Palavras-chave: Interdisciplinaridade; Ensino de Ciências; Ensino de Genética; O tempo e o vento; Érico Veríssimo.
Genética na Escola, May 4, 2019
Journal of Medicinal Plants Research, 2010
They describe in this paper that the dolastane diterpenes 4-hydroxy-9,14-dihydroxydolasta-1(15),7... more They describe in this paper that the dolastane diterpenes 4-hydroxy-9,14-dihydroxydolasta-1(15),7-diene (1) and 4,7,14-trihydroxydolasta-1(15),8-diene (2), isolated from the marine brown alga Canistrocarpus cervicornis inhibited HSV-1 infection in Vero cells. The concentration required to inhibit HSV-1 replication was not cytotoxic. Their results suggest that the structures of diterpenes 1 and 2, dolastane diterpenes from Brazilian brown algae, are promising for future antiviral drugs. Key words: Canistrocarpus cervicornis, dictyotaceae, phaeophyceae, dolastane diterpene, cytotoxicity, antiviral against HSV-1.
Revista Brasileira de Farmacognosia, 2011
The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linea... more The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linearol/isolinearol) that were isolated from the marine brown alga Canistrocarpus cervicornis were evaluated against biological activities of Lachesis muta snake venom. In vitro assays showed that the crude extract and the diterpenes were able to inhibit the clotting and proteolytic activity induced by L. muta crude venom, but not the hemolytic activity. However, only the diterpenes inhibited the hemolysis caused by a purified phospholipase A 2 previously isolated from L. muta venom, denoted LM-PLA 2-I. Interestingly, the crude algal extract and the diterpenes were able to protect mice from hemorrhage induced by L. muta venom. Thus, we may conclude that marine algae are rich and powerful sources of molecules that may be used against L. muta accidents in order to improve treatment of envenomation by this snake.
Revista Ibero-Americana de Estudos em Educação
A contemporaneidade se caracteriza pela celeridade e pela falta de tempo para ler; tal peculiarid... more A contemporaneidade se caracteriza pela celeridade e pela falta de tempo para ler; tal peculiaridade motiva uma escassa dedicação aos textos clássicos, os quais são reconhecidamente capazes de promover uma experiência humanizadora. Neste estudo, buscamos avaliar a utilização da obra Cem Anos de Solidão em um projeto escolar interdisciplinar no qual a literatura assumiu a posição de eixo integrador das áreas de conhecimento. O projeto foi proposto aos docentes de uma escola pública do estado do Rio de Janeiro e adaptado para aplicação. A intervenção foi avaliada através da análise de conteúdo das redações produzidas pelos alunos e de questionários aplicados aos docentes. O papel integrador do projeto proporcionou ao aluno/leitor o desprendimento da realidade imediata, o exercício da imaginação, ampliação da compreensão e de visão de mundo. A intervenção foi positivamente avaliada pelos alunos e professores e o estímulo à leitura de outros clássicos foi fomentando.
Ciência & Educação (Bauru)
Resumo: Discutimos o impacto da utilização da ficção científica, associada ao enfoque Ciência, Te... more Resumo: Discutimos o impacto da utilização da ficção científica, associada ao enfoque Ciência, Tecnologia e Sociedade (CTS), no desenvolvimento crítico dos alunos e o consequente despertar para o exercício da cidadania. Para tal, aplicamos uma sequência didática para alunos do ensino Médio, baseada na obra Admirável mundo novo, em uma perspectiva Freireana. A atividade é fundamentada na discussão, a partir de material de apoio preparado para os temas CTS destacados da obra: consumismo, uso de drogas, manipulação genética e exclusão social. A intervenção foi avaliada por meio de questionário. Verificamos o desenvolvimento do senso crítico dos alunos através das relações das temáticas CTS da sociedade contemporânea com a sociedade criada por Aldous Huxley em 1932. Os alunos perceberam a atividade como interdisciplinar, relacionando os temas abordados com várias disciplinas, e valorizaram a prática pedagógica alternativa. Os estudantes também reconheceram que as temáticas expostas e de...
Revista Aproximando, 2015
ABSTRACT Marine organisms are rich sources of natural products that, among other activities, help... more ABSTRACT Marine organisms are rich sources of natural products that, among other activities, help to maintain the species equilibrium. Samples of the marine brown alga Canistrocarpus cervicornis (Kutzing) De Paula & De Clerck were collected in Buzios, Rio de Janeiro, Brazil, in June 2006. The extract was obtained in CH2Cl2 and subjected to fractionation by chromatographic methods in order to isolate and purify the compound (4R, 7R, 14S)-4 alpha, 7 alpha-diacethoxy-14-hydroxydolastane-1(15), 8-dien. Then, the effects of the extract and the dolastane diterpene on zygotes and gametes of the sea urchin Lytechinus variegatus were evaluated. The exposure of male and female gametes to the C. cervicornis extract promoted, respectively, a reduction of 10-30% in fertilization and a 20 to 70% decrease in the number of eggs. Furthermore, the exposure of zygotes to the extract inhibited their development up to 86.7 +/- 1.6% (at a concentration of 250 mu g mL(-1)), as well as generating abnormalities in 39-50% of zygotes. The results of the dolastane diterpene showed no evidence of inhibition in the zygotes' development, thought it was proved to induce anomalies. At higher concentrations (25 and 50 mu g mL(-1)), it was possible to observe cell lyses.
Molecules, 2015
Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wid... more Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wide range of toxic effects. Envenomation by Bothrops jararaca venom results in hemorrhage, edema, pain, tissue necrosis and hemolysis. In this work, the effect of a mixture of two secodolastane diterpenes (linearol/isolinearol), previously isolated from the Brazilian marine brown alga, Canistrocarpus cervicornis, was evaluated against some of the toxic effects induced by B. jararaca venom. The mixture of diterpenes was dissolved in dimethylsulfoxide and incubated with venom for 30 min at room temperature, and then several in vivo (hemorrhage, edema and lethality) and in vitro (hemolysis, plasma clotting and proteolysis) assays were performed. The diterpenes inhibited hemolysis, proteolysis and hemorrhage, but failed to inhibit clotting and edema induced by B. jararaca venom. Moreover, diterpenes partially protected mice from lethality caused by B. jararaca venom. The search for natural inhibitors of B. jararaca venom in C. cervicornis algae is a relevant subject, since seaweeds are a rich and powerful source of active molecules which are as yet but poorly explored. Our results suggest that
Revista Brasileira de Farmacognosia, 2011
The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linea... more The effect of a Brazilian algae extract and also a mixture of two secodolastane diterpenes (linearol/isolinearol) that were isolated from the marine brown alga Canistrocarpus cervicornis were evaluated against biological activities of Lachesis muta snake venom. In vitro assays showed that the crude extract and the diterpenes were able to inhibit the clotting and proteolytic activity induced by L. muta crude venom, but not the hemolytic activity. However, only the diterpenes inhibited the hemolysis caused by a purified phospholipase A 2 previously isolated from L. muta venom, denoted LM-PLA 2-I. Interestingly, the crude algal extract and the diterpenes were able to protect mice from hemorrhage induced by L. muta venom. Thus, we may conclude that marine algae are rich and powerful sources of molecules that may be used against L. muta accidents in order to improve treatment of envenomation by this snake.
Brazilian Journal of Oceanography, 2007
Crude extracts of the brown seaweed Dictyota mertensii (Martius) Kützing collected at two distant... more Crude extracts of the brown seaweed Dictyota mertensii (Martius) Kützing collected at two distant and different places on the Brazilian coast, Búzios (Rio de Janeiro) and Fernando de Noronha (PE), were evaluated for defensive chemistry against the crab Pachygrapsus transversus, and the sea urchin Lytechinus variegatus. The extract from Búzios specimens of D. mertensii significantly inhibited the consumption by both P. transversus and L. varigetaus. Fractionation of the extracts of specimens of D. mertensii from Búzios and F. de Noronha followed by complementary assays revealed one active fraction from each location, which contained distinct defensive secondary metabolites. In each active fractions prenylated guaiane diterpenes were the major compounds. Dictyol H and epoxypachydictyol A were the most abundant compounds in Búzios and F. de Noronha, respectively, followed by minor components. Our results show a differential production of secondary metabolites in the two distant and dif...
Biochemical Systematics and Ecology, 2005
... diets and particular food chains, indicating specific herbivorous–algae relationships ( [Kele... more ... diets and particular food chains, indicating specific herbivorous–algae relationships ( [Kelecom and Teixeira, 1986] and [Pereira and Teixeira, 1999 ... The “Dictyota” group would include the genera Dictyota Lamouroux, Dilophus J. Agardh, Glossophora J. Agardh, Pachydictyon J ...
Revista Brasileira de Farmacognosia, 2011
Dictyotaceae are a rich source of secondary metabolites, especially terpenes. These natural produ... more Dictyotaceae are a rich source of secondary metabolites, especially terpenes. These natural products have been studied for their bioactivity for human and for their ecological role in nature. The present work highlights de diversity of the Dictyotaceae terpenes, emphasizing their bioactivities and the biogeography of their sources. The sesquiterpenes are found in Padina, Dictyopteris and Taonia. Although Dictyopteris and Padina can be found in all oceans, Taonia has a more restricted distribution. Diterpenes of mixed origin have been reported in Lobophora, Taonia and Stypopodium. Stypopodium is a typically tropical and warm temperate genus. Diterpenes with the spatane and seco-spatanes skeletons are known from Spatoglossum, Stoechospermum and Rugulopteryx. Spatoglossum is distributed over all the tropical marine world. Stoechospermum is known to occur in the Indian Ocean and Rugulopteryx is distributed mainly in Asia and on the Pacific Coast of Australia. The genus Dictyota contains diterpenes as the major metabolite and are present in all oceans, reaching even the cold temperate regions of northern and southern hemispheres. The terpenoids from Dictyotaceae exhibit bioactivities such as inhibition of herbivores and antifungal, cytotoxic, antibiotic, anti-inflammatory, insecticidal and antiviral activities.