Renato D. de Castro | UFBA - Federal University of Bahia (original) (raw)

Papers by Renato D. de Castro

Research paper thumbnail of Osmopriming-associated genes in Poincianella pyramidalis

Environmental and Experimental Botany

Research paper thumbnail of Sequence analysis of Ricinus communis small heat-shock protein (sHSP) subfamily and its role in abiotic stress responses

Industrial Crops and Products

Research paper thumbnail of Overexpression of Ricinus communis L. malate synthase enhances seed tolerance to abiotic stress during germination

Industrial Crops and Products

Research paper thumbnail of Root architecture system of oilseed species from the Jatropha genus during seed development and germination

Industrial Crops and Products

Research paper thumbnail of Identification of antioxidant and antimicrobial compounds from the oilseed crop Ricinus communis using a multiplatform metabolite profiling approach

Industrial Crops and Products

Research paper thumbnail of Chemical constituents of the oilseed crop Ricinus communis and their pharmacological activities: A review

Industrial Crops and Products

Research paper thumbnail of Phytochemical Screening, Antioxidant and Antibacterial Activities of Extracts Prepared from Different Tissues of Schinus terebinthifolius Raddi that Occurs in the Coast of Bahia, Brazil

Pharmacognosy Magazine, 2015

Medicinal, herbal and aromatic plants constitute a large segment of the flora, which provide raw ... more Medicinal, herbal and aromatic plants constitute a large segment of the flora, which provide raw materials for use by pharmaceutical, cosmetic, fragrance and flavor industries. They have been used in the country for a long time for their medicinal properties. Extracts of Methanol, chloroform and Benzene were further tested for antimicrobial activity against selective organisms. The Aegle marmelos leaves of methanolic extracts shows high antimicrobial activity against Escherichia coli (12mm) and Klebsiella sp (11mm). Minimum inhibitory concentration Staphylococcus aureus (0.023). Twenty compounds were detected in the Methanolic extract Stigmasterol (14.4925 %), Hexadecanoic acid (13.2053%). Antioxidant activity DPPH free radical scavenging activity. Plants have been used because of their antimicrobial traits, which are due to compounds synthesized in the secondary metabolism of the plant; such as phenols, terpenoids, alkaloids and flavonoids. Synergism between plant extract and synthetic antibiotics can develop standardization of herbal medicine for treatment and prevention of infectious diseases.

Research paper thumbnail of Effect of osmopriming on germination and initial growth of Physalis angulata L. under salt stress and on expression of associated genes

Anais da Academia Brasileira de Ciências, 2016

This study aimed to evaluate the effects of priming on seed germination under salt stress and gen... more This study aimed to evaluate the effects of priming on seed germination under salt stress and gene expression in seeds and seedlings of P. angulata L. After priming for 10 days, seed germination was tested in plastic trays containing 15 ml of water (0 dS m-1 - control) or 15 ml of NaCl solution (2, 4, 6, 8, 10, 12, 14 and 16 dS m-1). Fresh and dry weight of shoots and roots of seedlings were evaluated at 0, 2, 4, 6, 8 dS m-1. Total RNA was extracted from whole seeds and seedlings followed by RT-qPCR. The target genes selected for this study were: ascorbate peroxidase (APX), glutathione-S-transferase (GST), thioredoxin (TXN), high affinity potassium transporter protein 1 (HAK1) and salt overly sensitive 1 (SOS1). At an electroconductivity of 14 dS m-1 the primed seeds still germinated to 72%, in contrast with the non-primed seeds which did not germinate. The relative expression of APX was higher in primed seeds and this may have contributed to the maintenance of high germination in p...

Research paper thumbnail of Efeito da salinidade na germina��o de sementes e no crescimento inicial de mudas de pinh�o-manso

Revista Brasileira De Sementes, 2010

Research paper thumbnail of Detection of �-tubulin in tomato seeds: Optimization of extraction and immunodetection

Research paper thumbnail of Influência de métodos e épocas de colheita sobre a produção e qualidade de sementes de Brachiaria decumbens CV. "Basilisk

Revista Brasileira de Sementes, 1994

ABSTRACT

Research paper thumbnail of Metabolite profiling of Ricinus communis germination at different temperatures provides new insights into thermo-mediated requirements for successful seedling establishment

Plant Science, 2015

Ricinus communis seeds germinate to a high percentage and faster at 35°C than at lower temperatur... more Ricinus communis seeds germinate to a high percentage and faster at 35°C than at lower temperatures, but with compromised seedling establishment. However, seedlings are able to cope with high temperatures at later stages of seedling establishment if germination occurred at lower temperatures. Our objective was to assess the biochemical and molecular requirements of R. communis germination for successful seedling establishment at varying temperatures. For that, we performed metabolite profiling (GC-TOF-MS) and measured transcript levels of key genes involved in several energy-generating pathways, such as storage oil mobilization, β-oxidation and gluconeogenesis of seeds germinated at three different temperatures. We identified a thermo-sensitive window during seed germination in which high temperatures compromise seedling development, most likely by down-regulating some energy-generating pathways. Overexpression of malate synthase (MLS) and glycerol kinase (GK) genes resulted in higher starch levels in Nicotiana benthamiana leaves, which highlights the importance of these genes in energy-generating pathways for seedling establishment. Additionally, we showed that GABA, which is a stress-responsive metabolite, accumulated in response to the water content of the seeds during the initial phase of imbibition. Herewith, we provide new insights into the molecular requirements for vigorous seedling growth of R. communis under different environmental conditions.

Research paper thumbnail of Quality Standards for Seed Germination: The Use of Nuclear Dna Amounts to Predict Storage Behavior and Germination Rate

III International Symposium Diversification of Vegetable Crops, 1998

Research paper thumbnail of Effect of temperature on biomass allocation in seedlings of two contrasting genotypes of the oilseed crop Ricinus communis

Journal of Plant Physiology, 2015

Ricinus communis is becoming an important crop for oil production, and studying the physiological... more Ricinus communis is becoming an important crop for oil production, and studying the physiological and biochemical aspects of seedling development may aid in the improvement of crop quality and yield. The objective of this study was to assess the effect of temperature on biomass allocation in two R. communis genotypes. Biomass allocation was assessed by measuring dry weight of roots, stems, and cotyledons of seedlings grown at three different temperatures. Root length of each seedling was measured. Biomass allocation was strongly affected by temperature. Seedlings grown at 25°C and 35°C showed greater biomass than seedlings grown at 20°C. Cotyledon and stem dry weight increased for both genotypes with increasing temperature, whereas root biomass allocation showed a genotype-dependent behavior. Genotype MPA11 showed a continuous increase in root dry weight with increasing temperature, while genotype IAC80 was not able to sustain further root growth at higher temperatures. Based on metabolite and gene expression profiles, genotype MPA11 increases its level of osmoprotectant molecules and transcripts of genes encoding for antioxidant enzymes and heat shock proteins to a higher extent than genotype IAC80. This might be causal for the ability to maintain homeostasis and support root growth at elevated temperatures in genotype MPA11.

Research paper thumbnail of Cells cylcle events in developing neem (Azadirachta indica) seeds: Are they related to intermediate storage behavior?

Research paper thumbnail of Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compounds

Food Research International, 2015

Aos meus pais, Arthur e Denise, que sempre me apoiaram em tudo e procuraram buscar para mim o mel... more Aos meus pais, Arthur e Denise, que sempre me apoiaram em tudo e procuraram buscar para mim o melhor. À Profª Rosane Nora Castro, pela orientação, dedicação, amizade e pelo exemplo de profissionalismo. Ao Profº Aurélio Baird Buarque Ferreira, pela amizade, ensinamentos e estímulo na continuação do meu trabalho. Aos professores do PPGQ que contribuiram para meu crescimento profissional. Às amigas Luiza Sant'Ana, Juliana Paes Leme e Aline Lira pela amizade, companheirismo, força e alegria durante toda a execução desde trabalho. Aos demais amigos de laboratório Leonardo, Mariana, Dominique, Lucas e Vitor Sueth pelo convívio no Laboratório 48B. Ao André Canuto, pelas discussões que ajudaram a aliviar as tensões ao longo da execução deste trabalho. À banca examinadora, por aceitar o convite e pelas futuras contribuições. À UFRRJ e ao PPGQ, pela oportunidade e qualidade de ensino. À CAPES, FAPERJ e CNPq pelo apoio financeiro E, por fim, agradeço a Deus, por ter criado as abelhas e a diversidade das flores, e ter nos dado os sentidos para apreciar tudo isso.

Research paper thumbnail of DNA synthesis and beta-tubulin expression in embryos of developing tomato (Lycopersicon esculentum) seeds

Research paper thumbnail of b -Tubulin accumulation and DNA synthesis are sequentially resumed in embryo organs of cucumber (Cucumis sativus L.) seeds during germination

Research paper thumbnail of Cell Cycle Analysis in Dormant and Germinating Tomato Seeds

Current Plant Science and Biotechnology in Agriculture, 1997

ffiÍï:"ïí*l$*.*. Ki..' corrc!. t!ndo'. c'mpd" trirr Ro'd' trn&n wl 7^lÍ. ttt(KLUWE,R ACADEMÍC PUB... more ffiÍï:"ïí*l$*.*. Ki..' corrc!. t!ndo'. c'mpd" trirr Ro'd' trn&n wl 7^lÍ. ttt(KLUWE,R ACADEMÍC PUBLISHERS ÍloRoRFfrlÍ / 06ÍoN, aoNooN 43. Cell Cycle Analysis in Dormant and Germínating Tomalo Seeds s.p.c cRooT'. R.D. oE cÁsÍRo'r. Y Lru" |íd R J. BtNo' t6a'ta t-tU 84&...1 ÈFíJÉne rdeó (CrrO-OapL O4-14d 4 rí''n4dííl ,tud .t, EA 6r b ,,@,a,r atriin tÍ. l\rtÍ.'t & 'tht-dt a tutrnl* aN (lmtl ,rq-t-e & tu 6a ,áí-5-&rÉr c| t\d n, ad trc, C6t ,rf.n. ara:'O.F,i.dd rr.d-. tÈ 4ddLa *r.. W.ntE |k Ah|.a C.ll .Flc .dlíty i|. pítrlqt|bc Íd t@tt rd &wloFÍ.iÍ. In !ÉfriMti.t r..d3, c.ll cFb divlt t.t t sr óoatr to F!.td. Í!iU! t.mlndd. Tt |rFt @io 6{ .r.L r!..Íó *ló óoíns d ar.trJrttlt ton!..o !..& N{ck Í DNA tt tr(io1 ...r't'e.í br lb' crtdd.Í .1..b í.llE anft.Fic.d íe .nd F€.ó.r vËbl. Ítnli..iio!. Ttk DNA adi..tiod .bo 6Ít d'r.h!

Research paper thumbnail of Physiological and biochemical responses of

BMC Plant Biology

Background Compared with major crops, growth and development of Ricinus communis is still poorly ... more Background Compared with major crops, growth and development of Ricinus communis is still poorly understood. A better understanding of the biochemical and physiological aspects of germination and seedling growth is crucial for the breeding of high yielding varieties adapted to various growing environments. In this context, we analysed the effect of temperature on growth of young R. communis seedlings and we measured primary and secondary metabolites in roots and cotyledons. Three genotypes, recommended to small family farms as cash crop, were used in this study.ResultsSeedling biomass was strongly affected by the temperature, with the lowest total biomass observed at 20°C. The response in terms of biomass production for the genotype MPA11 was clearly different from the other two genotypes: genotype MPA11 produced heavier seedlings at all temperatures but the root biomass of this genotype decreased with increasing temperature, reaching the lowest value at 35°C. In contrast, root biom...

Research paper thumbnail of Osmopriming-associated genes in Poincianella pyramidalis

Environmental and Experimental Botany

Research paper thumbnail of Sequence analysis of Ricinus communis small heat-shock protein (sHSP) subfamily and its role in abiotic stress responses

Industrial Crops and Products

Research paper thumbnail of Overexpression of Ricinus communis L. malate synthase enhances seed tolerance to abiotic stress during germination

Industrial Crops and Products

Research paper thumbnail of Root architecture system of oilseed species from the Jatropha genus during seed development and germination

Industrial Crops and Products

Research paper thumbnail of Identification of antioxidant and antimicrobial compounds from the oilseed crop Ricinus communis using a multiplatform metabolite profiling approach

Industrial Crops and Products

Research paper thumbnail of Chemical constituents of the oilseed crop Ricinus communis and their pharmacological activities: A review

Industrial Crops and Products

Research paper thumbnail of Phytochemical Screening, Antioxidant and Antibacterial Activities of Extracts Prepared from Different Tissues of Schinus terebinthifolius Raddi that Occurs in the Coast of Bahia, Brazil

Pharmacognosy Magazine, 2015

Medicinal, herbal and aromatic plants constitute a large segment of the flora, which provide raw ... more Medicinal, herbal and aromatic plants constitute a large segment of the flora, which provide raw materials for use by pharmaceutical, cosmetic, fragrance and flavor industries. They have been used in the country for a long time for their medicinal properties. Extracts of Methanol, chloroform and Benzene were further tested for antimicrobial activity against selective organisms. The Aegle marmelos leaves of methanolic extracts shows high antimicrobial activity against Escherichia coli (12mm) and Klebsiella sp (11mm). Minimum inhibitory concentration Staphylococcus aureus (0.023). Twenty compounds were detected in the Methanolic extract Stigmasterol (14.4925 %), Hexadecanoic acid (13.2053%). Antioxidant activity DPPH free radical scavenging activity. Plants have been used because of their antimicrobial traits, which are due to compounds synthesized in the secondary metabolism of the plant; such as phenols, terpenoids, alkaloids and flavonoids. Synergism between plant extract and synthetic antibiotics can develop standardization of herbal medicine for treatment and prevention of infectious diseases.

Research paper thumbnail of Effect of osmopriming on germination and initial growth of Physalis angulata L. under salt stress and on expression of associated genes

Anais da Academia Brasileira de Ciências, 2016

This study aimed to evaluate the effects of priming on seed germination under salt stress and gen... more This study aimed to evaluate the effects of priming on seed germination under salt stress and gene expression in seeds and seedlings of P. angulata L. After priming for 10 days, seed germination was tested in plastic trays containing 15 ml of water (0 dS m-1 - control) or 15 ml of NaCl solution (2, 4, 6, 8, 10, 12, 14 and 16 dS m-1). Fresh and dry weight of shoots and roots of seedlings were evaluated at 0, 2, 4, 6, 8 dS m-1. Total RNA was extracted from whole seeds and seedlings followed by RT-qPCR. The target genes selected for this study were: ascorbate peroxidase (APX), glutathione-S-transferase (GST), thioredoxin (TXN), high affinity potassium transporter protein 1 (HAK1) and salt overly sensitive 1 (SOS1). At an electroconductivity of 14 dS m-1 the primed seeds still germinated to 72%, in contrast with the non-primed seeds which did not germinate. The relative expression of APX was higher in primed seeds and this may have contributed to the maintenance of high germination in p...

Research paper thumbnail of Efeito da salinidade na germina��o de sementes e no crescimento inicial de mudas de pinh�o-manso

Revista Brasileira De Sementes, 2010

Research paper thumbnail of Detection of �-tubulin in tomato seeds: Optimization of extraction and immunodetection

Research paper thumbnail of Influência de métodos e épocas de colheita sobre a produção e qualidade de sementes de Brachiaria decumbens CV. "Basilisk

Revista Brasileira de Sementes, 1994

ABSTRACT

Research paper thumbnail of Metabolite profiling of Ricinus communis germination at different temperatures provides new insights into thermo-mediated requirements for successful seedling establishment

Plant Science, 2015

Ricinus communis seeds germinate to a high percentage and faster at 35°C than at lower temperatur... more Ricinus communis seeds germinate to a high percentage and faster at 35°C than at lower temperatures, but with compromised seedling establishment. However, seedlings are able to cope with high temperatures at later stages of seedling establishment if germination occurred at lower temperatures. Our objective was to assess the biochemical and molecular requirements of R. communis germination for successful seedling establishment at varying temperatures. For that, we performed metabolite profiling (GC-TOF-MS) and measured transcript levels of key genes involved in several energy-generating pathways, such as storage oil mobilization, β-oxidation and gluconeogenesis of seeds germinated at three different temperatures. We identified a thermo-sensitive window during seed germination in which high temperatures compromise seedling development, most likely by down-regulating some energy-generating pathways. Overexpression of malate synthase (MLS) and glycerol kinase (GK) genes resulted in higher starch levels in Nicotiana benthamiana leaves, which highlights the importance of these genes in energy-generating pathways for seedling establishment. Additionally, we showed that GABA, which is a stress-responsive metabolite, accumulated in response to the water content of the seeds during the initial phase of imbibition. Herewith, we provide new insights into the molecular requirements for vigorous seedling growth of R. communis under different environmental conditions.

Research paper thumbnail of Quality Standards for Seed Germination: The Use of Nuclear Dna Amounts to Predict Storage Behavior and Germination Rate

III International Symposium Diversification of Vegetable Crops, 1998

Research paper thumbnail of Effect of temperature on biomass allocation in seedlings of two contrasting genotypes of the oilseed crop Ricinus communis

Journal of Plant Physiology, 2015

Ricinus communis is becoming an important crop for oil production, and studying the physiological... more Ricinus communis is becoming an important crop for oil production, and studying the physiological and biochemical aspects of seedling development may aid in the improvement of crop quality and yield. The objective of this study was to assess the effect of temperature on biomass allocation in two R. communis genotypes. Biomass allocation was assessed by measuring dry weight of roots, stems, and cotyledons of seedlings grown at three different temperatures. Root length of each seedling was measured. Biomass allocation was strongly affected by temperature. Seedlings grown at 25°C and 35°C showed greater biomass than seedlings grown at 20°C. Cotyledon and stem dry weight increased for both genotypes with increasing temperature, whereas root biomass allocation showed a genotype-dependent behavior. Genotype MPA11 showed a continuous increase in root dry weight with increasing temperature, while genotype IAC80 was not able to sustain further root growth at higher temperatures. Based on metabolite and gene expression profiles, genotype MPA11 increases its level of osmoprotectant molecules and transcripts of genes encoding for antioxidant enzymes and heat shock proteins to a higher extent than genotype IAC80. This might be causal for the ability to maintain homeostasis and support root growth at elevated temperatures in genotype MPA11.

Research paper thumbnail of Cells cylcle events in developing neem (Azadirachta indica) seeds: Are they related to intermediate storage behavior?

Research paper thumbnail of Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compounds

Food Research International, 2015

Aos meus pais, Arthur e Denise, que sempre me apoiaram em tudo e procuraram buscar para mim o mel... more Aos meus pais, Arthur e Denise, que sempre me apoiaram em tudo e procuraram buscar para mim o melhor. À Profª Rosane Nora Castro, pela orientação, dedicação, amizade e pelo exemplo de profissionalismo. Ao Profº Aurélio Baird Buarque Ferreira, pela amizade, ensinamentos e estímulo na continuação do meu trabalho. Aos professores do PPGQ que contribuiram para meu crescimento profissional. Às amigas Luiza Sant'Ana, Juliana Paes Leme e Aline Lira pela amizade, companheirismo, força e alegria durante toda a execução desde trabalho. Aos demais amigos de laboratório Leonardo, Mariana, Dominique, Lucas e Vitor Sueth pelo convívio no Laboratório 48B. Ao André Canuto, pelas discussões que ajudaram a aliviar as tensões ao longo da execução deste trabalho. À banca examinadora, por aceitar o convite e pelas futuras contribuições. À UFRRJ e ao PPGQ, pela oportunidade e qualidade de ensino. À CAPES, FAPERJ e CNPq pelo apoio financeiro E, por fim, agradeço a Deus, por ter criado as abelhas e a diversidade das flores, e ter nos dado os sentidos para apreciar tudo isso.

Research paper thumbnail of DNA synthesis and beta-tubulin expression in embryos of developing tomato (Lycopersicon esculentum) seeds

Research paper thumbnail of b -Tubulin accumulation and DNA synthesis are sequentially resumed in embryo organs of cucumber (Cucumis sativus L.) seeds during germination

Research paper thumbnail of Cell Cycle Analysis in Dormant and Germinating Tomato Seeds

Current Plant Science and Biotechnology in Agriculture, 1997

ffiÍï:"ïí*l$*.*. Ki..' corrc!. t!ndo'. c'mpd" trirr Ro'd' trn&n wl 7^lÍ. ttt(KLUWE,R ACADEMÍC PUB... more ffiÍï:"ïí*l$*.*. Ki..' corrc!. t!ndo'. c'mpd" trirr Ro'd' trn&n wl 7^lÍ. ttt(KLUWE,R ACADEMÍC PUBLISHERS ÍloRoRFfrlÍ / 06ÍoN, aoNooN 43. Cell Cycle Analysis in Dormant and Germínating Tomalo Seeds s.p.c cRooT'. R.D. oE cÁsÍRo'r. Y Lru" |íd R J. BtNo' t6a'ta t-tU 84&...1 ÈFíJÉne rdeó (CrrO-OapL O4-14d 4 rí''n4dííl ,tud .t, EA 6r b ,,@,a,r atriin tÍ. l\rtÍ.'t & 'tht-dt a tutrnl* aN (lmtl ,rq-t-e & tu 6a ,áí-5-&rÉr c| t\d n, ad trc, C6t ,rf.n. ara:'O.F,i.dd rr.d-. tÈ 4ddLa *r.. W.ntE |k Ah|.a C.ll .Flc .dlíty i|. pítrlqt|bc Íd t@tt rd &wloFÍ.iÍ. In !ÉfriMti.t r..d3, c.ll cFb divlt t.t t sr óoatr to F!.td. Í!iU! t.mlndd. Tt |rFt @io 6{ .r.L r!..Íó *ló óoíns d ar.trJrttlt ton!..o !..& N{ck Í DNA tt tr(io1 ...r't'e.í br lb' crtdd.Í .1..b í.llE anft.Fic.d íe .nd F€.ó.r vËbl. Ítnli..iio!. Ttk DNA adi..tiod .bo 6Ít d'r.h!

Research paper thumbnail of Physiological and biochemical responses of

BMC Plant Biology

Background Compared with major crops, growth and development of Ricinus communis is still poorly ... more Background Compared with major crops, growth and development of Ricinus communis is still poorly understood. A better understanding of the biochemical and physiological aspects of germination and seedling growth is crucial for the breeding of high yielding varieties adapted to various growing environments. In this context, we analysed the effect of temperature on growth of young R. communis seedlings and we measured primary and secondary metabolites in roots and cotyledons. Three genotypes, recommended to small family farms as cash crop, were used in this study.ResultsSeedling biomass was strongly affected by the temperature, with the lowest total biomass observed at 20°C. The response in terms of biomass production for the genotype MPA11 was clearly different from the other two genotypes: genotype MPA11 produced heavier seedlings at all temperatures but the root biomass of this genotype decreased with increasing temperature, reaching the lowest value at 35°C. In contrast, root biom...