Rosiane Teles | Universidade Federal do Ceará (original) (raw)

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Papers by Rosiane Teles

Research paper thumbnail of Interferon-gamma gene polymorphism influences the frequency of a Chlamydia trachomatis cervical infection in young women

International journal of STD & AIDS, Jan 12, 2014

Summary INTRODUCTION: Cervicitis associated with Chlamydia trachomatis is frequent worldwide, but... more Summary INTRODUCTION: Cervicitis associated with Chlamydia trachomatis is frequent worldwide, but the factors determining susceptibility to infection remain incompletely determined. We evaluated whether a functional single nucleotide polymorphism at position +874 in the gene coding for interferon gamma (rs2430561) influenced the likelihood of having a cervical C. trachomatis infection. This was a cross-sectional study of 142 sexually active women attending a general gynaecology service on the outskirts of the city of Fortaleza in northeastern Brazil between August 2011 and August 2012. Endocervical swabs were evaluated for C. trachomatis DNA using hybrid capture. DNA from buccal swabs was utilised for detection of the interferon gamma 874 T/A single nucleotide polymorphism by gene amplification, endonuclease digestion and gel electrophoresis. Associations were analysed by Fisher's exact test. Odds ratios and 95% confidence intervals were determined. Nineteen women (13.4%) were p...

Research paper thumbnail of Anemia Aplástica e Gravidez: Relato de Caso

Revista Brasileira de Ginecologia e Obstetrícia, 2002

Research paper thumbnail of Association of TP53 codon 72 and intron 3 16-bp Ins/Del polymorphisms with cervical cancer risk

Tumor Biology, 2014

Cervical cancer incidence has grown worldwide, with it being a more significant problem in develo... more Cervical cancer incidence has grown worldwide, with it being a more significant problem in developing countries. Invasive squamous cell cervical cancers are preceded by a long phase of preinvasive disease, known as cervical intraepithelial neoplasia. Cervical cancer can develop when the virus takes advantage of any TP53 gene dysfunction of the host organism. TP53 is responsible for encoding the tumor suppressor p53 phosphoprotein, which helps preserve genome integrity. Currently, many studies have focused on genetic polymorphisms as an important contribution to cancer susceptibility, but few related to cervical intraepithelial neoplasia (CIN). Thus, the present study aimed to see whether patients with suspected CIN had TP53 gene polymorphisms that might have contributed to the development of neoplasia. This study included 133 women who were referred to the Cervical Pathology Clinic of the Maternity School Assis Chateaubriand MEAC for suspected cervical lesions. Polymorphism genotyping was carried out by the PCR-RFLP technique using DNA extracted from patients' blood. The most frequent genotype in both CIN(+) and CIN(−) patients was Arg/Pro TP53 codon 72 and A1A1 for 16-bp Del in intron 3. No risk of cervical cancer was found for the polymorphisms studied.

Research paper thumbnail of Interferon-gamma gene polymorphism influences the frequency of a Chlamydia trachomatis cervical infection in young women

International journal of STD & AIDS, Jan 12, 2014

Summary INTRODUCTION: Cervicitis associated with Chlamydia trachomatis is frequent worldwide, but... more Summary INTRODUCTION: Cervicitis associated with Chlamydia trachomatis is frequent worldwide, but the factors determining susceptibility to infection remain incompletely determined. We evaluated whether a functional single nucleotide polymorphism at position +874 in the gene coding for interferon gamma (rs2430561) influenced the likelihood of having a cervical C. trachomatis infection. This was a cross-sectional study of 142 sexually active women attending a general gynaecology service on the outskirts of the city of Fortaleza in northeastern Brazil between August 2011 and August 2012. Endocervical swabs were evaluated for C. trachomatis DNA using hybrid capture. DNA from buccal swabs was utilised for detection of the interferon gamma 874 T/A single nucleotide polymorphism by gene amplification, endonuclease digestion and gel electrophoresis. Associations were analysed by Fisher's exact test. Odds ratios and 95% confidence intervals were determined. Nineteen women (13.4%) were p...

Research paper thumbnail of Anemia Aplástica e Gravidez: Relato de Caso

Revista Brasileira de Ginecologia e Obstetrícia, 2002

Research paper thumbnail of Association of TP53 codon 72 and intron 3 16-bp Ins/Del polymorphisms with cervical cancer risk

Tumor Biology, 2014

Cervical cancer incidence has grown worldwide, with it being a more significant problem in develo... more Cervical cancer incidence has grown worldwide, with it being a more significant problem in developing countries. Invasive squamous cell cervical cancers are preceded by a long phase of preinvasive disease, known as cervical intraepithelial neoplasia. Cervical cancer can develop when the virus takes advantage of any TP53 gene dysfunction of the host organism. TP53 is responsible for encoding the tumor suppressor p53 phosphoprotein, which helps preserve genome integrity. Currently, many studies have focused on genetic polymorphisms as an important contribution to cancer susceptibility, but few related to cervical intraepithelial neoplasia (CIN). Thus, the present study aimed to see whether patients with suspected CIN had TP53 gene polymorphisms that might have contributed to the development of neoplasia. This study included 133 women who were referred to the Cervical Pathology Clinic of the Maternity School Assis Chateaubriand MEAC for suspected cervical lesions. Polymorphism genotyping was carried out by the PCR-RFLP technique using DNA extracted from patients' blood. The most frequent genotype in both CIN(+) and CIN(−) patients was Arg/Pro TP53 codon 72 and A1A1 for 16-bp Del in intron 3. No risk of cervical cancer was found for the polymorphisms studied.

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