Flávia F Aburjaile | UFMG - The Federal University of Minas Gerais (original) (raw)

Papers by Flávia F Aburjaile

International Journal of Infectious Diseases, 2019

This study delineated the clonal lineages, antibiotic resistome and plasmid replicon types in mul... more This study delineated the clonal lineages, antibiotic resistome and plasmid replicon types in multidrug-resistant K. pneumoniae isolates from a teaching hospital in Ghana. Methods: Identification and antibiotic susceptibility testing were done using the MALDI-TOF MS and Vitek-2 automated system. Genomic DNA extraction was carried out using the NucliSens easyMAG1 (BioMérieux) kits and the DNA was subjected to whole genome sequencing (WGS) using the Illumina MiSeq platform. Results: Of the 200 isolates obtained, 37 were identified as K. pneumoniae of which 9 were resistant to all second and third-generation cephalosporins. These 9 isolates selected for further genomic analysis were characterized by the presence of 8 diverse sequence types (STs), capsular polysaccharide serotypes (K types and wzi allelic types) and multiple genes encoding resistance to β-lactams (bla CTX-M-15 , bla SHV-11, bla TEM-1B, bla OXA-1 ), aminoglycosides (aac(3)-IIa, strB, strA, aadA16), fluoroquinolones/quinolones (qnrB66, oqxA, oqxB) and other antibiotic classes. Resistance genes were associated with plasmids, predominantly IncFIB(K) and ColRNAI. Multiple and diverse mutations in quinolone resistance-determining regions of gyrA (S83Y, D87A) and parC (S80I, N304S) in isolates resistant to ciprofloxacin (MIC 4 mg/mL) were found. Global phylogenomic analysis affirmed the diverse clonal clustering and origin of these isolates. Conclusions: The varied clonal clusters and resistome identified in the multidrug-resistant K. pneumoniae isolates is a major threat to the management of infections in Ghana. The molecular characterization of antibiotic resistance is thus imperative to inform strategies for containment.

Journal of Biotechnology, Aug 1, 2013

Corynebacterium pseudotuberculosis is the causative agent of several veterinary diseases in a bro... more Corynebacterium pseudotuberculosis is the causative agent of several veterinary diseases in a broad range of economically important hosts, which can vary from caseous lymphadenitis in sheep and goats (biovar ovis) to ulcerative lymphangitis in cattle and horses (biovar equi). Existing vaccines against C. pseudotuberculosis are mainly intended for small ruminants and, even in these hosts, they still present remarkable limitations. In this study, we present the complete genome sequence of C. pseudotuberculosis biovar equi strain 258, isolated from a horse with ulcerative lymphangitis. The genome has a total size of 2,314,404 bp and contains 2088 predicted protein-coding regions. Using in silico analysis, eleven pathogenicity islands were detected in the genome sequence of C. pseudotuberculosis 258. The application of a reverse vaccinology strategy identified 49 putative antigenic proteins, which can be used as candidate vaccine targets in future works.

Probiotics and Antimicrobial Proteins

Microbiology resource announcements, Dec 2, 2021

Pseudomonas sp. strain LAP_36 was isolated from rhizosphere soil from Deschampsia antarctica on K... more Pseudomonas sp. strain LAP_36 was isolated from rhizosphere soil from Deschampsia antarctica on King George Island, South Shetland Islands, Antarctica. Here, we report on its draft genome sequence, which consists of 8,794,771 bp with 60.0% GC content and 8,011 protein-coding genes.

HAL (Le Centre pour la Communication Scientifique Directe), Nov 16, 2016

Propionibacterium freudenreichii is a bacterial species belonging to Actinobacteria philum, known... more Propionibacterium freudenreichii is a bacterial species belonging to Actinobacteria philum, known for its survival in long periods under adverse environmental conditions. The selection of starters propionic is a major economic challenge. This bacterium is widely used in dairy industry for the ripening process of Swiss-Type cheeses, such as Emmental, which production exceeds 250,000 tonnes/year in France. This bacteria also presents potent strain-specific probiotic effects and produces biomolecules of industrial interest. The survivability of P. freudenreichii is essential to its role during ripening (several months) despite strong environmental change, such as pH, nutrient shortage, temperature. Survival is also an advantage for its probiotic applications for resistance to gastric pH and bile salts.

Journal of Bacteriology, Dec 1, 2012

This work reports the completion and annotation of the genome sequence of Corynebacterium pseudot... more This work reports the completion and annotation of the genome sequence of Corynebacterium pseudotuberculosis I19, isolated from an Israeli dairy cow with severe clinical mastitis. To present the whole-genome sequence, a de novo assembly approach using 33 million short (25-bp) mate-paired SOLiD reads only was applied. Furthermore, the automatic, functional, and manual annotations were attained with the use of several algorithms in a multistep process.

Antibiotics, Jun 11, 2023

O presente estudo se propos a validar transcritos WRKY via PCR quantitativa em tempo real (RT-qPC... more O presente estudo se propos a validar transcritos WRKY via PCR quantitativa em tempo real (RT-qPCR) em acessos de videira infectados com X. citri em tres tempos distintos (90 minutos, 24 e 48 horas) apos a inoculacao com a bacteria.

O presente trabalho objetivou validar a expressao diferencial de genes NBS-LRR candidatos via PCR... more O presente trabalho objetivou validar a expressao diferencial de genes NBS-LRR candidatos via PCR quantitativa em tempo real (RT-qPCR) em acessos de videira contrastantes quanto a resistencia a X. citri.

Revista de Biologia e Ciências da Terra, 2011

Journal of Bacteriology, Dec 15, 2011

Frontiers in bioinformatics, Feb 7, 2023

medRxiv (Cold Spring Harbor Laboratory), Apr 4, 2021

doi: medRxiv preprint NOTE: This preprint reports new research that has not been certified by pee... more doi: medRxiv preprint NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice.

Antibiotics, Mar 21, 2023

Phytopathology, Jan 26, 2023

Cashew (Anacardium occidentale L.) angular leaf spot is caused by pigmented and non-pigmented str... more Cashew (Anacardium occidentale L.) angular leaf spot is caused by pigmented and non-pigmented strains of Xanthomonas citri pv. anacardii (Ah-You et al. 2007) Gama et al. 2018, which have been isolated from infected plants in Brazil (Gama et al. 2018; Gama et al. 2011). The disease was first reported by Robbs et al. (1981), infecting cashew trees in the Brazilian Northeast and Southeast regions. The leaf symptoms include angular spots and dark lesions in the veins and surrounding tissues. The vein darkening moves from the main vein to the secondary veins (Viana et al. 2007) and the petioles, causing extensive dry necrosis of twigs and their tips (Gama et al. 2011). On the fruit, dark necrotic lesions are usually observed (Viana et al. 2007). So far, no symptoms have been observed in the pseudofruit (Gama et al. 2011). Given that infection in young fruits results in fruits unsuitable for commercialization, the angular leaf spot represents a serious threat to the cashew crop in Brazil (Viana et al., 2007; Gama et al. 2018), especially in the Northeast, as this region produces approximately 99% of the Brazilian cashew nuts (IBGE 2022). In addition, there is no data on the losses caused by this pathogen in the country. In this study, we report the genomic sequencing of seven pigmented strains of X. citri pv. anacardii, obtained from the leaves of cashew trees from São Paulo state, Brazil, in 2009, and previously characterized by biochemical, molecular, and pathological techniques (Gama et al. 2011; Gama et al. 2018). The draft genome of these strains will be useful to clarify different aspects of this important bacterium, and it will help minimize the damage caused to the cashew crop. The strains CCRMTAQ9, CCRMTAQ11, CCRMTAQ14, CCRMTAQ18, CCRMTAQ24, CCRMTAQ29, and CCRMTAQ31 were cultured in NYDA medium (20 g agar, 10 g dextrose, 5 g peptone, 5 g yeast extract, and 3 g meat extract, completing the volume with sterile distilled water [SDW] to 1,000 ml) and incubated for 36 h at 29 °C. DNA from the strains was extracted using the MiniPrep bacterial DNA extraction kit (Axygen Biosciences, MA), according to the manufacturer's

Authorea (Authorea), Aug 13, 2023

Corynebacterium spp. have emerged as opportunistic pathogens, with increasing reports of isolatio... more Corynebacterium spp. have emerged as opportunistic pathogens, with increasing reports of isolation from patients with mild and severe infections. Corynebacterial isolates were found expressing multidrug-resistant (MDR) phenotypes in most cases. In the present work, we report the isolation of an MDR C. glucuronolyticum strain from the human urogenital tract. In addition, we provide comprehensive genomic data of the isolate that was first identified by mass spectrometry (MALDI-TOF) and had its antimicrobial susceptibility profile determined by the disc diffusion method. Genomic analyses were applied to confirm the species identification and predict genes involved in the virulence, antimicrobial resistance, and CRISPR-Cas systems. C. glucuronolyticum isolate exhibited resistance to benzylpenicillin, clindamycin, and tetracycline. Two virulence (mprA and tlyC) and several antimicrobial genes, including those related to tetracycline and lincosamides, were found in the genome. Additionally, the Type I-E CRISPR-Cas system was detected. Although C. glucuronolyticum has been isolated from patients with human infections, mostly male patients with genitourinary tract infections, its pathogenic potential is unclear. Presently, the clinical resolution only occurred with antimicrobial treatment considering our results, reinforcing that the antimicrobial susceptibility profile should be performed in cases of infection caused by Corynebacterium spp., especially in the face of the emergence of MDR isolates. Moreover, our data revealed putative virulence and antimicrobial resistance factors, which should be better characterized to understand the pathogenicity potential of C. glucuronolyticum for the genitourinary tract. RESEARCH PAPER Phenotypic characterization and whole genome analysis of a multidrug-resistant Corynebacterium glucuronolyticum strain isolated from genitourinary tract for the first time in Latin America

Research in Microbiology, Jun 1, 2023

Bacteria, Oct 6, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Genetics and Molecular Research, 2021

Lactobacilli are the predominant bacterial species colonizing the vaginal surfaces of healthy wom... more Lactobacilli are the predominant bacterial species colonizing the vaginal surfaces of healthy women, where they play a protective role against opportunistic and polymicrobial infections, such as bacterial vaginosis. Several Lactobacillus species, especially L. crispatus, have been prospected for probiotic applications due to their potential antimicrobial and anti-inflammatory capacities. During the last decade, several genomic studies have been investigating the genetics of L. crispatus strains in an effort to identify novel probiotic strains and evaluate their potential for improving human and animal health. This mini review highlights the main genes associated with L. crispatus protective mechanisms in four novel strains of this species that we recently isolated from healthy Brazilian women of reproductive age. Among the probiotic features of these strains, the roles of a pyruvate oxidase-encoding gene, lactate synthesis related enzymes, bacteriocin genes, and genomic islands, are reviewed, and the next steps for confirming their activity are indicated.