Lucas Pessatto | UFMS - Universidade Federal de Mato Grosso do Sul (original) (raw)

Papers by Lucas Pessatto

Journal of Ethnopharmacology, Mar 1, 2017

Effects of dichloromethane and butanol fractions of Gochnatia polymorpha subsp.

Alimentos funcionais ricos em β-glucanas podem associar-se com atividades biologicas tais como a ... more Alimentos funcionais ricos em β-glucanas podem associar-se com atividades biologicas tais como a prevencao de danos mutagenicos e alteracoes no desempenho reprodutivo e desenvolvimento embriofetal. Frente ao exposto a presente pesquisa avaliou os efeitos da suplementacao de Avena sativa L. no desenvolvimento ponderal, desempenho reprodutivo e desenvolvimento embriofetal de ratas prenhes expostas a ciclofosfamida. Os resultados indicaram que a suplementacao com farelo de aveia e capaz de reduzir o ganho de peso durante a gestacao. Mas nao interfere no desempenho reprodutivo e desenvolvimento embriofetal. Verificou-se ainda que a suplementacao com farelo de aveia nao e capaz de prevenir os efeitos adversos causados pela administracao da ciclofosfamida. Assim, sugere-se que a biodisponibilidade de β-glucana, na suplementacao

O extrato etanolico de Moquiniastrum polymorphum subsp. Floccosum, suas fracoes e compostos isola... more O extrato etanolico de Moquiniastrum polymorphum subsp. Floccosum, suas fracoes e compostos isolados possuem atividade anti-inflamatoria em ambos os sexos. O extrato etanolico dessa planta ainda foi indicada para a bioprospeccao de um anti-inflamatorio fitoterapico para uso durante a gestacao por nao apresentar efeito mutagenico, nao alterar o desempenho reprodutivo e nem o desenvolvimento embriofetal o que sugere seguranca no seu uso. Frente ao exposto a presente pesquisa avaliou os efeitos das fracoes diclorometano (DCM) e butanolica (BT) de M. polymorphum em camundongos femeas prenhes. Os animais foram tratados em periodo pre-implantacao (1-4 dia gestacional (dg)), de organogenese (5-15dg) e gestacional (1-18dg) com as doses de 300mg/kg e 100mg/kg para DCM e BT, respectivamente. As analises estatisticas demonstraram que a DCM e abortiva ou impede a nidacao nos tres protocolos testados sendo sua acao mais pronunciada no protocolo de pre-implantacao (p 0,05). Os resultados sugerem ...

Archives of Head and Neck Surgery, 2020

Financial support: None. Conflicts of interest: No conflicts of interest declared concerning the ... more Financial support: None. Conflicts of interest: No conflicts of interest declared concerning the publication of this article.

Inorganica Chimica Acta, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

International Journal of Pharmacy Practice, 2019

Objective The objective of this study was to evaluate the frequency of genetic lesions in pharmac... more Objective The objective of this study was to evaluate the frequency of genetic lesions in pharmacists and nurses who prepare and/or handle antineoplastic agents and to evaluate whether there are traces of contaminants in the urine of these professionals. Methods A total of 59 professionals participated in the study, of which 10 were non-exposed professionals (controls), 25 were pharmacists, and 24 were nurses. Key findings There was a significant increase in genetic damage in lymphocytes and cells of the oral mucosa in both pharmacists and nurses. The levels of cyclophosphamide and ifosfamide were also increased in the urine samples from those individuals. Conclusions: These results demonstrate the growing need for genetic biomonitoring and biomonitoring of trace antineoplastic agents in the urine of health professionals who prepare and/or handle antineoplastic agents.

Brazilian Archives of Biology and Technology, 2019

Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and ... more Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was performed employing an in-house method based on guanidine thiocyanate and magnetic bead. For the amplification it was used the Quantifier Y Kit™. The fetal sexing analysis of the 132 pregnant women were 100% in agreement with the ultrasound. Sensitivity and specificity for detection of Y chromosome sequences was possible using Real-Time Polymerase Chain Reaction since the 4th week of gestation. This non-invasive early determination could be employed in fetal gender and also to be extended to detection of genetic diseases in the shortest possible time avoiding invasive methods that puts the fetus at risk.

Environmental Science and Pollution Research, 2019

The amphyphylic triazoanilines recently synthesized 1-(4-(3-aminophenyl)-1H-1,2,3-triazole-1-yl)-... more The amphyphylic triazoanilines recently synthesized 1-(4-(3-aminophenyl)-1H-1,2,3-triazole-1-yl)-3-(3pentadecylphenoxy)propan-2-ol (1) and 1-(4-(4-aminophenyl)-1H-1,2,3-triazole-1-yl)-3-(3-pentadecylphenoxy)propan-2-ol (2), synthesized from cardanol and glycerol, have photophysical properties which allow their use in the development of fluorescent biomarkers with applicability in the biodiesel quality control. Based on this, the present research evaluated the toxic effects of both compounds in different biological models through the investigation of survival and mortality percentages as a measure of acute toxicity on Daphnia similis and Oreochromis niloticus, larvicidal assay against Aedes aegypti, and cytotoxic activity on mammary cells. Results demonstrate that these triazoanilines 1 and 2 have shown low acute toxicity to the biological models investigated in this study up to the following concentrations: 4.0 mg L-1 (D. similis), 4.0 mg L-1 (A. aegypti larvae), 1.0 mg L-1 (O. niloticus), and 1.0 mg mL-1 (mammary cells). This fact suggests the potential for safe use of compounds 1 and 2 as fluorescent markers for the monitoring of biodiesel quality, even in the case of environmental exposure. Besides all of that, the reuse of cardanol and glycerol, both industrial wastes, favors the maintenance of environmental health and is in agreement with the assumptions of green chemistry.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 26, 2018

Calcitriol, the bioactive hormone of vitamin D, is currently linked to several diseases, such as ... more Calcitriol, the bioactive hormone of vitamin D, is currently linked to several diseases, such as obesity and gain of adipose mass, due to its liposolubility and, consequently, its sequestration by adipocytes. As rates of obesity continue to increase, research on the biology of weight gain should be encouraged. This study evaluated the effects of calcitriol combined with CaCl on adipose tissue-derived human mesenchymal stem cells. We evaluated the cytotoxicity of the combination by MTT assays, in which undifferentiated cells and cells undergoing adipogenic differentiation were tested for 7 and 14 days. The results demonstrated that the combination of calcitriol at the IC and CaCl at the IC was effective at reducing the viability of mesenchymal stem cells, but with the progression of cell differentiation towards adipocytes, cell resistance to the cytotoxic effects increased. The percentages of dead cells were 88.29, 57.45 and 28.81% for undifferentiated cells and cells exposed to diff...

Life sciences, Jan 10, 2018

Previous studies performed by our research group indicated that cytosporone analogues are capable... more Previous studies performed by our research group indicated that cytosporone analogues are capable of prevent or repair DNA damages. This work presents the evaluation of the activity of AMS35AA for metastatic murine melanoma cells (B16F10) in experimental model in vitro and, in pre-clinic assay of metastatic melanoma in vivo, using mice lineage C57BL/6. In vitro assays were performed: MTT and comet assay, flow cytometry evaluation, gene expression assay by RT-PCR, qualitative evaluation of cell death using B16F10 cells. In vivo assays: micronucleus and comet assay, splenic phagocytosis, melanoma murine model and histopathological analysis, using mice lineage C57BL/6 (n = 20). In vitro results performed by MTT assay showed that AMS35AA is cytotoxic for B16F10 cells (p < 0.05). Based on comet assay the genotoxicity of the IC was determined (95.83 μg/mL) (p < 0.05). These data were corroborated by flow cytometry analysis after the treatment with AMS35AA, which indicates the cellul...

Toxicology and applied pharmacology, Jan 6, 2018

Copper (II) complexes are promising in the development of new synthetic models for cancer treatme... more Copper (II) complexes are promising in the development of new synthetic models for cancer treatment. In this context, we synthesized a new copper complex containing the pharmacophore group 1,4-dioxo-2-butenyl, the Bis(((Z)-4-((4-chlorophenyl) amino)-4-oxobut-2-enoyl)oxy) copper compound and we evaluated its antitumor activity in 4 T1 murine mammary adenocarcinoma cells and their toxicogenic effect in Swiss mice. The compound demonstrated cytotoxicity and genotoxicity to 4 T1 cells, and after cell cycle arrest in G1, which occurred by the increase in ATM and p21 expression, it induced the cells to apoptosis by increasing BAX and caspase-7. In vivo the compound was genotoxic in mice but did not show permanent damage, observed by the absence of increased micronucleus frequency, and did not induce changes in the biometric parameters of the animals. These results indicate that the new copper complex, described firstly in this work, presents therapeutic potential for breast cancer.

Genetics and Molecular Research, 2017

Colorectal cancer is a global public health issue. Studies have pointed to the protective effect ... more Colorectal cancer is a global public health issue. Studies have pointed to the protective effect of probiotics on colorectal carcinogenesis. Activia ® is a lacto probiotic product that is widely consumed all over the world and its beneficial properties are related, mainly, to the lineage of traditional yoghurt bacteria combined with a specific bacillus, DanRegularis, which gives the product a proven capacity to intestinal regulation in humans. The aim of this study was to evaluate the antigenotoxic, antimutagenic, and anticarcinogenic proprieties of the Activia product, in response to damage caused by 1,2-dimethylhydrazine (DMH) in Swiss mice. Activia does not have shown antigenotoxic activity. However, the percent of DNA damage reduction, evaluated by the antimutagenicity assay, ranged from 69.23 to 96.15% indicating effective chemopreventive action. Activia reduced up to 79.82% the induction of aberrant crypt foci by DMH. Facing the results, it is inferred that Activia facilitates the weight loss, prevents DNA damage and pre-cancerous lesions in the intestinal mucosa.

Genetics and Molecular Research, 2017

The objective of this study was to evaluate the effect of Moquiniastrum polymorphum ssp floccosum... more The objective of this study was to evaluate the effect of Moquiniastrum polymorphum ssp floccosum ethanolic extract (MPEE) on 1,2 dimethylhydrazine (DMH)-induced colorectal carcinogenesis in mice. Forty-two male Swiss mice (Mus musculus) were subdivided into six groups (N = 7/group): negative control, DMH, MPEE, pre-treatment,

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 11, 2017

Studies have shown that metabolic disorders, serum inflammatory markers and weight gain (obesity)... more Studies have shown that metabolic disorders, serum inflammatory markers and weight gain (obesity) are correlated with vitamin D deficiency. Therefore, the present study correlated the serum calcidiol (s25(OH)D3) levels in a sample of individuals from southern Brazil with variables related to metabolic disorders, obesity and lifestyle habits and assessed the cytotoxic effect of calcitriol on adipose tissue-derived mesenchymal stem cells (ADSCs). The results showed a 79.23% prevalence of hypovitaminosis D in the study population and a correlation (p<0.05) between a low serum vitamin D concentration and an elevated low-density lipoprotein cholesterol (LDL-c) level. Univariate linear regression analysis using 25(OH)D3 as a regressor showed a negative association (p<0.05) with an indoor work environment (β=-2.305), increased body fat (β=-0.095), age (β=-0.065) and high-density lipoprotein cholesterol (HDL-c; β=-0.109). An in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu...

Genetics and Molecular Research, 2016

Acrocomia aculeata is a plant rich in antioxidant compounds. Studies suggest that this plant has ... more Acrocomia aculeata is a plant rich in antioxidant compounds. Studies suggest that this plant has anti-inflammatory, antidiabetic, and diuretic potential. We assessed the antigenotoxic, antimutagenic, immunomodulation, and apoptotic potentials of A. aculeata alone and in combination with an antitumor agent, cyclophosphamide. Swiss male mice (N = 140) were used. The animals were divided into 14 experimental groups as follows: a negative group, a positive group (100 mg/kg cyclophosphamide), groups that only M.F. Magosso et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (2): gmr.15027816 received the oil extracted from the almond (AO) and from the pulp (PO) of A. aculeata at doses of 3, 15, and 30 mg/kg, and the associated treatment groups (oils combined with cyclophosphamide) involving pretreatment, simultaneous, and post-treatment protocols. Data suggest that both oils were chemopreventive at all doses, based on the tested protocols. The highest damage reduction percentages, observed for AO and PO were 88.19 and 90.03%, respectively, for the comet assay and 69.73 and 70.93%, respectively, for the micronucleus assay. Both AO and PO demonstrated immunomodulatory activity. The oils reduced the capacity of cyclophosphamide to trigger apoptosis in the liver, spleen, and kidney cells. These results suggest that A. aculeate AO and PO can be classified as a functional food and also enrich other functional foods and nutraceuticals with chemopreventive features. However, they are not appropriate sources for chemotherapeutic adjuvants, in particular for those used in combination with cyclophosphamide.

Journal of Ethnopharmacology, 2017

Effects of dichloromethane and butanol fractions of Gochnatia polymorpha subsp.

Journal of Ethnopharmacology, Mar 1, 2017

Effects of dichloromethane and butanol fractions of Gochnatia polymorpha subsp.

Alimentos funcionais ricos em β-glucanas podem associar-se com atividades biologicas tais como a ... more Alimentos funcionais ricos em β-glucanas podem associar-se com atividades biologicas tais como a prevencao de danos mutagenicos e alteracoes no desempenho reprodutivo e desenvolvimento embriofetal. Frente ao exposto a presente pesquisa avaliou os efeitos da suplementacao de Avena sativa L. no desenvolvimento ponderal, desempenho reprodutivo e desenvolvimento embriofetal de ratas prenhes expostas a ciclofosfamida. Os resultados indicaram que a suplementacao com farelo de aveia e capaz de reduzir o ganho de peso durante a gestacao. Mas nao interfere no desempenho reprodutivo e desenvolvimento embriofetal. Verificou-se ainda que a suplementacao com farelo de aveia nao e capaz de prevenir os efeitos adversos causados pela administracao da ciclofosfamida. Assim, sugere-se que a biodisponibilidade de β-glucana, na suplementacao

O extrato etanolico de Moquiniastrum polymorphum subsp. Floccosum, suas fracoes e compostos isola... more O extrato etanolico de Moquiniastrum polymorphum subsp. Floccosum, suas fracoes e compostos isolados possuem atividade anti-inflamatoria em ambos os sexos. O extrato etanolico dessa planta ainda foi indicada para a bioprospeccao de um anti-inflamatorio fitoterapico para uso durante a gestacao por nao apresentar efeito mutagenico, nao alterar o desempenho reprodutivo e nem o desenvolvimento embriofetal o que sugere seguranca no seu uso. Frente ao exposto a presente pesquisa avaliou os efeitos das fracoes diclorometano (DCM) e butanolica (BT) de M. polymorphum em camundongos femeas prenhes. Os animais foram tratados em periodo pre-implantacao (1-4 dia gestacional (dg)), de organogenese (5-15dg) e gestacional (1-18dg) com as doses de 300mg/kg e 100mg/kg para DCM e BT, respectivamente. As analises estatisticas demonstraram que a DCM e abortiva ou impede a nidacao nos tres protocolos testados sendo sua acao mais pronunciada no protocolo de pre-implantacao (p 0,05). Os resultados sugerem ...

Archives of Head and Neck Surgery, 2020

Financial support: None. Conflicts of interest: No conflicts of interest declared concerning the ... more Financial support: None. Conflicts of interest: No conflicts of interest declared concerning the publication of this article.

Inorganica Chimica Acta, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

International Journal of Pharmacy Practice, 2019

Objective The objective of this study was to evaluate the frequency of genetic lesions in pharmac... more Objective The objective of this study was to evaluate the frequency of genetic lesions in pharmacists and nurses who prepare and/or handle antineoplastic agents and to evaluate whether there are traces of contaminants in the urine of these professionals. Methods A total of 59 professionals participated in the study, of which 10 were non-exposed professionals (controls), 25 were pharmacists, and 24 were nurses. Key findings There was a significant increase in genetic damage in lymphocytes and cells of the oral mucosa in both pharmacists and nurses. The levels of cyclophosphamide and ifosfamide were also increased in the urine samples from those individuals. Conclusions: These results demonstrate the growing need for genetic biomonitoring and biomonitoring of trace antineoplastic agents in the urine of health professionals who prepare and/or handle antineoplastic agents.

Brazilian Archives of Biology and Technology, 2019

Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and ... more Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was performed employing an in-house method based on guanidine thiocyanate and magnetic bead. For the amplification it was used the Quantifier Y Kit™. The fetal sexing analysis of the 132 pregnant women were 100% in agreement with the ultrasound. Sensitivity and specificity for detection of Y chromosome sequences was possible using Real-Time Polymerase Chain Reaction since the 4th week of gestation. This non-invasive early determination could be employed in fetal gender and also to be extended to detection of genetic diseases in the shortest possible time avoiding invasive methods that puts the fetus at risk.

Environmental Science and Pollution Research, 2019

The amphyphylic triazoanilines recently synthesized 1-(4-(3-aminophenyl)-1H-1,2,3-triazole-1-yl)-... more The amphyphylic triazoanilines recently synthesized 1-(4-(3-aminophenyl)-1H-1,2,3-triazole-1-yl)-3-(3pentadecylphenoxy)propan-2-ol (1) and 1-(4-(4-aminophenyl)-1H-1,2,3-triazole-1-yl)-3-(3-pentadecylphenoxy)propan-2-ol (2), synthesized from cardanol and glycerol, have photophysical properties which allow their use in the development of fluorescent biomarkers with applicability in the biodiesel quality control. Based on this, the present research evaluated the toxic effects of both compounds in different biological models through the investigation of survival and mortality percentages as a measure of acute toxicity on Daphnia similis and Oreochromis niloticus, larvicidal assay against Aedes aegypti, and cytotoxic activity on mammary cells. Results demonstrate that these triazoanilines 1 and 2 have shown low acute toxicity to the biological models investigated in this study up to the following concentrations: 4.0 mg L-1 (D. similis), 4.0 mg L-1 (A. aegypti larvae), 1.0 mg L-1 (O. niloticus), and 1.0 mg mL-1 (mammary cells). This fact suggests the potential for safe use of compounds 1 and 2 as fluorescent markers for the monitoring of biodiesel quality, even in the case of environmental exposure. Besides all of that, the reuse of cardanol and glycerol, both industrial wastes, favors the maintenance of environmental health and is in agreement with the assumptions of green chemistry.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 26, 2018

Calcitriol, the bioactive hormone of vitamin D, is currently linked to several diseases, such as ... more Calcitriol, the bioactive hormone of vitamin D, is currently linked to several diseases, such as obesity and gain of adipose mass, due to its liposolubility and, consequently, its sequestration by adipocytes. As rates of obesity continue to increase, research on the biology of weight gain should be encouraged. This study evaluated the effects of calcitriol combined with CaCl on adipose tissue-derived human mesenchymal stem cells. We evaluated the cytotoxicity of the combination by MTT assays, in which undifferentiated cells and cells undergoing adipogenic differentiation were tested for 7 and 14 days. The results demonstrated that the combination of calcitriol at the IC and CaCl at the IC was effective at reducing the viability of mesenchymal stem cells, but with the progression of cell differentiation towards adipocytes, cell resistance to the cytotoxic effects increased. The percentages of dead cells were 88.29, 57.45 and 28.81% for undifferentiated cells and cells exposed to diff...

Life sciences, Jan 10, 2018

Previous studies performed by our research group indicated that cytosporone analogues are capable... more Previous studies performed by our research group indicated that cytosporone analogues are capable of prevent or repair DNA damages. This work presents the evaluation of the activity of AMS35AA for metastatic murine melanoma cells (B16F10) in experimental model in vitro and, in pre-clinic assay of metastatic melanoma in vivo, using mice lineage C57BL/6. In vitro assays were performed: MTT and comet assay, flow cytometry evaluation, gene expression assay by RT-PCR, qualitative evaluation of cell death using B16F10 cells. In vivo assays: micronucleus and comet assay, splenic phagocytosis, melanoma murine model and histopathological analysis, using mice lineage C57BL/6 (n = 20). In vitro results performed by MTT assay showed that AMS35AA is cytotoxic for B16F10 cells (p < 0.05). Based on comet assay the genotoxicity of the IC was determined (95.83 μg/mL) (p < 0.05). These data were corroborated by flow cytometry analysis after the treatment with AMS35AA, which indicates the cellul...

Toxicology and applied pharmacology, Jan 6, 2018

Copper (II) complexes are promising in the development of new synthetic models for cancer treatme... more Copper (II) complexes are promising in the development of new synthetic models for cancer treatment. In this context, we synthesized a new copper complex containing the pharmacophore group 1,4-dioxo-2-butenyl, the Bis(((Z)-4-((4-chlorophenyl) amino)-4-oxobut-2-enoyl)oxy) copper compound and we evaluated its antitumor activity in 4 T1 murine mammary adenocarcinoma cells and their toxicogenic effect in Swiss mice. The compound demonstrated cytotoxicity and genotoxicity to 4 T1 cells, and after cell cycle arrest in G1, which occurred by the increase in ATM and p21 expression, it induced the cells to apoptosis by increasing BAX and caspase-7. In vivo the compound was genotoxic in mice but did not show permanent damage, observed by the absence of increased micronucleus frequency, and did not induce changes in the biometric parameters of the animals. These results indicate that the new copper complex, described firstly in this work, presents therapeutic potential for breast cancer.

Genetics and Molecular Research, 2017

Colorectal cancer is a global public health issue. Studies have pointed to the protective effect ... more Colorectal cancer is a global public health issue. Studies have pointed to the protective effect of probiotics on colorectal carcinogenesis. Activia ® is a lacto probiotic product that is widely consumed all over the world and its beneficial properties are related, mainly, to the lineage of traditional yoghurt bacteria combined with a specific bacillus, DanRegularis, which gives the product a proven capacity to intestinal regulation in humans. The aim of this study was to evaluate the antigenotoxic, antimutagenic, and anticarcinogenic proprieties of the Activia product, in response to damage caused by 1,2-dimethylhydrazine (DMH) in Swiss mice. Activia does not have shown antigenotoxic activity. However, the percent of DNA damage reduction, evaluated by the antimutagenicity assay, ranged from 69.23 to 96.15% indicating effective chemopreventive action. Activia reduced up to 79.82% the induction of aberrant crypt foci by DMH. Facing the results, it is inferred that Activia facilitates the weight loss, prevents DNA damage and pre-cancerous lesions in the intestinal mucosa.

Genetics and Molecular Research, 2017

The objective of this study was to evaluate the effect of Moquiniastrum polymorphum ssp floccosum... more The objective of this study was to evaluate the effect of Moquiniastrum polymorphum ssp floccosum ethanolic extract (MPEE) on 1,2 dimethylhydrazine (DMH)-induced colorectal carcinogenesis in mice. Forty-two male Swiss mice (Mus musculus) were subdivided into six groups (N = 7/group): negative control, DMH, MPEE, pre-treatment,

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 11, 2017

Studies have shown that metabolic disorders, serum inflammatory markers and weight gain (obesity)... more Studies have shown that metabolic disorders, serum inflammatory markers and weight gain (obesity) are correlated with vitamin D deficiency. Therefore, the present study correlated the serum calcidiol (s25(OH)D3) levels in a sample of individuals from southern Brazil with variables related to metabolic disorders, obesity and lifestyle habits and assessed the cytotoxic effect of calcitriol on adipose tissue-derived mesenchymal stem cells (ADSCs). The results showed a 79.23% prevalence of hypovitaminosis D in the study population and a correlation (p<0.05) between a low serum vitamin D concentration and an elevated low-density lipoprotein cholesterol (LDL-c) level. Univariate linear regression analysis using 25(OH)D3 as a regressor showed a negative association (p<0.05) with an indoor work environment (β=-2.305), increased body fat (β=-0.095), age (β=-0.065) and high-density lipoprotein cholesterol (HDL-c; β=-0.109). An in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu...

Genetics and Molecular Research, 2016

Acrocomia aculeata is a plant rich in antioxidant compounds. Studies suggest that this plant has ... more Acrocomia aculeata is a plant rich in antioxidant compounds. Studies suggest that this plant has anti-inflammatory, antidiabetic, and diuretic potential. We assessed the antigenotoxic, antimutagenic, immunomodulation, and apoptotic potentials of A. aculeata alone and in combination with an antitumor agent, cyclophosphamide. Swiss male mice (N = 140) were used. The animals were divided into 14 experimental groups as follows: a negative group, a positive group (100 mg/kg cyclophosphamide), groups that only M.F. Magosso et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (2): gmr.15027816 received the oil extracted from the almond (AO) and from the pulp (PO) of A. aculeata at doses of 3, 15, and 30 mg/kg, and the associated treatment groups (oils combined with cyclophosphamide) involving pretreatment, simultaneous, and post-treatment protocols. Data suggest that both oils were chemopreventive at all doses, based on the tested protocols. The highest damage reduction percentages, observed for AO and PO were 88.19 and 90.03%, respectively, for the comet assay and 69.73 and 70.93%, respectively, for the micronucleus assay. Both AO and PO demonstrated immunomodulatory activity. The oils reduced the capacity of cyclophosphamide to trigger apoptosis in the liver, spleen, and kidney cells. These results suggest that A. aculeate AO and PO can be classified as a functional food and also enrich other functional foods and nutraceuticals with chemopreventive features. However, they are not appropriate sources for chemotherapeutic adjuvants, in particular for those used in combination with cyclophosphamide.

Journal of Ethnopharmacology, 2017

Effects of dichloromethane and butanol fractions of Gochnatia polymorpha subsp.