Rafael Guerra Lund | Universidade Federal de Pelotas (Federal University of Pelotas) (original) (raw)

Papers by Rafael Guerra Lund

Ciência, Tecnologia e Inovação: do campo à mesa, 2020

Resumo O leite é um produto altamente perecível e suas características físico-químicas e biológic... more Resumo O leite é um produto altamente perecível e suas características físico-químicas e biológicas se alteram facilmente com a ação de microrganismos. Casos de mastite e ma higienizacao no processo de ordenha posibilitam o aparecimento desses agentes. Como alternativa profilatica e terapêutica, as plantas medicinais ganham espaço na medicina veterinária como opção aos tratamentos e prevenções usuais. O objetivo deste estudo foi avaliar o potencial antibacteriano do extrato alcoólico de Jatropha dioica frente a bactérias isoladas de leite de vacas com mastite. A suscetibilidade de Staphylococcus spp. e Streptococcus spp., foi verificada através da técnica de Microdiluição em Caldo (CLSI, 2006). Observou-se inibição do crescimento dos isolados nas CIM de 28,12 µg mL-1 a 112,5 µg mL-1. Estes resultados sugerem potencial antimicrobiano do extrato alcoólico de J. dioica, caracterizando-a como promissora no tratamento e prevenção da mastite bovina e contaminação do leite.

Dansk Veterinaertidsskrift, 1980

Advances in human biology, 2020

Paediatric death inside dental office by the effect of anaesthesia in dentistry is a very difficu... more Paediatric death inside dental office by the effect of anaesthesia in dentistry is a very difficult case but not rare. In this article, we reviewed more than thirty studies with data focussing on death associated with dental anaesthesia using nitrous oxide (N2O). In children, most cases died at the age of 2–5 years. Hypoxia was the most common cause of death and cardiovascular, respiratory. Although rare death following general anaesthesia in dentistry, it is a critical side effect mostly seen in adult patients with compromised health conditions. Therefore, appropriate case selection in regard to patient's general health status as well as standard technical and equipment conditions is mandatory to reduce the risk of death during N2O dental anaesthesia in children. We have also given the list of guidelines and dietary care for children during N2O anaesthetic procedures inside the dental office.

Uma variedade de doencas envolve a cavidade oral, como a carie dental, considerada a patologia or... more Uma variedade de doencas envolve a cavidade oral, como a carie dental, considerada a patologia oral mais prevalente em todo o mundo. Atualmente, a carie dental e uma doenca multifatorial infecto-contagiosa, associada a bacterias patogenicas residentes do biofilme dental. Estes microrganismos sao os responsaveis pela producao de acidos e produtos citotoxicos que, respectivamente, levam a desmineralizacao de esmalte dental e inflamacao gengival com posterior destruicao dos tecidos de suporte dos dentes. Assim, o uso de agentes antimicrobianos eficientes contra essas bacterias e um importante meio de controle dessa doenca bucal. Varios agentes antimicrobianos de origem natural estao sendo investigados devido as suas possiveis propriedades farmacologicas. O cogumelo Agaricus brasiliensis S. Wasser et al. ( cogumelo-do-sol) destaca-se entre os produtos naturais com notaveis propriedades antibacterianas, antifungicas, antiinflamatorias e antitumorais. Neste estudo foi avaliado o efeito de tres extratos etanolicos brutos (EtOH 100%, 75% e 50%) deste cogumelo sobre o crescimento e a aderencia de estreptococos do grupo mutans. Os extratos etanolicos foram obtidos atraves do metodo de extracao por maceracao. Os controles negativos foram etanol 100%, 75% e 50%. Os testes antimicrobianos utilizados contra Streptococcus mutans UA 159 e Streptococcus sobrinus 6715 foram o metodo de difusao em agar, Concentracao Inibitoria Minima (CIM), Concentracao Bactericida Minima (CBM) e aderencia dos microrganismos a superficie de vidro (Adh). No metodo de difusao em agar, apenas o extrato 100% etanolico apresentou sinal de inibicao do crescimento microbiano. Ja nos testes de determinacao da CIM e CBM, os tres extratos apresentaram propriedades antimicrobianas e os valores de CIM e CBM variaram entre 87,4 e 444,5 μg/mL. A CBM para os tres extratos etanolicos foi igual a CIM para S.sobrinus, mas apenas o extrato etanolico 75% foi bactericida para S. mutans. Entre os extratos etanolicos testados, o extrato 100% etanolico foi o mais ativo (CIM 87.4μg/mL). A aderencia celular tambem foi inibida em concentracoes de 100 a 400μg/mL dos extratos obtidos com etanol 50% e 75%. Concluindo, os extratos etanolicos de A. brasiliensis mostraram notaveis efeitos inibitorios no crescimento bacteriano e o extrato 100% etanolico foi o mais ativo

Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2024

Objective: To review the literature unprecedentedly to identify the dentistry students' knowledge... more Objective: To review the literature unprecedentedly to identify the dentistry students' knowledge of pediatric patients who suffered violence. Material and Methods: A systematic review was conducted using PubMed, Web of Science, LILACS, SciELO, Google Scholar, and OpenGrey electronic databases up to November 2021. The "Grey Literature" was verified through Google Scholar and Open Grey searches to avoid any selection bias. There was no restriction on the date of publication or language. Results: The systematic search yielded 2.756 studies in the first selection phase, but only twenty-two articles were included. All selected articles were published between 1998 and 2021 and used a questionnaire to evaluate the dentistry students' knowledge regarding child maltreatment cases. Brazil was the country that had the most studies included (10 articles). Despite the majority of the students presenting insufficient knowledge about child maltreatment, evidence from this research showed that every form of approach by professionals toward child maltreatment should be considered important. Conclusion: Therefore, there are deficiencies regarding the teaching-learning methodology, reinforcing the need for improvements in Dentistry undergraduate curricula.

Brazilian Journal of Oral Sciences, Nov 29, 2023

Aim: This study aimed to investigate the prevalence of physical violence and head and neck injuri... more Aim: This study aimed to investigate the prevalence of physical violence and head and neck injuries among children and adolescents who suffered physical aggression, firearm aggression, and white gun aggression referred to the Legal Medical Department in a southern city in Brazil. Methods: This study was performed at Legal Medical Department in the city of Pelotas, RS, Brazil, from January 2011 to December of 2015. Data were collected from medical reports of children and adolescents (0-19 years old). Demographic information such as sex, age, and skin color was collected. Data were submitted to Chi-square test and multivariate Poisson regression analysis. Results: In this study, 2,716 physical examination reports were analyzed, with a total of 2,171 exams resulting from violence; lesions that had physical aggression as their etiology (1,951) had a higher prevalence. The analysis of the exams revealed that the majority were adolescents (90,05%), white (84.09%), and female (50.21%). The head and neck regions were affected in most cases of violence (57.90%). In the adjusted model, the occurrence of injuries in the head and neck region was higher in males (PR 1.16, CI 95% 1.08-1.25), among adolescents (PR 1.28, CI 95% 1.10-1.48) and in victims of physical aggression compared to victims of the firearm (2.81, CI 95% 1.79-4.40). Conclusion: The results revealed a high prevalence of head and face injuries in victims of violence and that there was a greater prevalence of physical violence among adolescents compared to children and males.

Revista Brasileira de Odontologia Legal - RBOL, Dec 6, 2020

Avaliação de danos pessoais em odontologia e discussão sobre seguro DPVATrelato de caso.

Revista da ABENO, Oct 31, 2020

Research Square (Research Square), Sep 28, 2022

This systematic review covers the existing evidence in the literature regarding the association o... more This systematic review covers the existing evidence in the literature regarding the association of propolis with controlled delivery systems (DDS) and its potential therapeutic action in dental medicine. This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Two independent reviewers performed a literature search up to 15th January 2022 in ve databases: PubMed/Medline, Web of Science, Cochrane Library, Scopus, and Embase, to identify the eligible studies. Clinical, in situ, and in vitro studies that investigated the incorporation of propolis as the main agent in DDS for dental medicine were included in this study. Conversly, review articles, clinical cases, theses, dissertations, abstracts at congresses, and studies that had no application in dentistry were excluded. After examining carefully 55 full-text articles, 11 in vitro studies, 1 animal model, and 1 in vivo and in vitro study were included (n = 13). Relevant data were extracted from the included studies and analyzed qualitatively. The use of propolis has been reported in cariology, endodontics periodontics, and implantology. Propolis has shown non-cytotoxic, osteoinductive, antimicrobial, and anti-in ammatory properties. Moreover, propolis can readly be released from DDS for prolonged periods, having potential biocompatibility, safety, and potential advantage for applications in dental medicine.

Journal of Prosthetic Dentistry, Apr 1, 2021

STATEMENT OF PROBLEM The high recurrence rates of denture stomatitis may be associated with the r... more STATEMENT OF PROBLEM The high recurrence rates of denture stomatitis may be associated with the resistance of biofilms to therapeutics. Therefore, methods that provide biomaterials with antifungal properties are an attractive solution to improving microbial control. PURPOSE The purpose of this in vitro study was to modify conventional polymethyl methacrylate (PMMA) through the incorporation of metal methacrylate monomers and to evaluate the physicomechanical and optical properties and antifungal activity of the modified materials. MATERIAL AND METHODS Experimental denture base acrylic resins were fabricated through the addition of zirconium methacrylate (ZM), tin methacrylate (TM), and di-n-butyldimethacrylate-tin (DNBMT) to the liquid of a commercially available denture base PMMA resin. Unmodified PMMA resin was used as the control. The degree of conversion of the materials was tested through Fourier transform infrared spectroscopy (n=3). A digital spectrophotometer was used to assess the color change of the modified materials (n=8). Differences in Knoop hardness and roughness between experimental groups were also evaluated (n=8). A biofilm accumulation test with Candida albicans (ATCC 62342) (n=4) was performed for 5 days in Sabouraud broth culture supplemented with 10% sucrose. Data were subjected to analysis of variance and the post hoc Tukey honestly significant difference test (α=.05). RESULTS The degree of conversion and color-change values of the experimental materials were statistically similar to those of the control (P=.593). The incorporation of DNBMT significantly increased the hardness of the modified material (P=.014). The ZM, TM, and DNBMT groups had higher antifungal activity against C. albicans (P=.001) and lower roughness than the control group (control 0.65 ±0.05 μm; ZM 0.34 ±0.09 μm, TM 0.34 ±0.11 μm, and DNBMT 0.41 ±0.08 μm). CONCLUSIONS The metal-containing methacrylate monomers provided antifungal action to the modified materials without affecting the physicomechanical or optical properties of the denture base resin. ZM, TM, and DNBMT are potential reactive agents for the fabrication of PMMA denture base resins with antifungal properties.

Biofouling, Apr 20, 2020

The objective of this study was to evaluate the physico-chemical and antimicrobial properties of ... more The objective of this study was to evaluate the physico-chemical and antimicrobial properties of a dual polymerization experimental endodontic sealer (E) and experimental sealers containing dibutyltin methacrylate (Sn2+) (ETs) or calcium methacrylate (Ca2+) (ECs). The pH and ion release levels of the sealers were measured. The dimensional stability was evaluated in accordance with ISO 6876. Biofilm growth inhibition was evaluated using confocal laser scanning microscopy (CLSM). Biofilm viability analysis was performed using the SYTO 9 technique. The shelf life was evaluated through the degree of conversion and film thickness tests after the sealers had been stored for different periods of time. For statistical analysis, ANOVA and Tukey's post hoc test were used, with a significance level of 5%. ETs revealed better anti-biofilm potential after 15 days than that of the controls. The degree of conversion was reduced after the shelf-life period. The addition of calcium and dibutyltin methacrylate improved the anti-biofilm properties of the experimental endodontic sealer without impairing their physico-chemical properties.

Journal of Ethnopharmacology, Apr 1, 2022

ETHNOPHARMACOLOGICAL RELEVANCE Bixa orellana L. is reported to have numerous applications in trad... more ETHNOPHARMACOLOGICAL RELEVANCE Bixa orellana L. is reported to have numerous applications in traditional medicine and pharmacological properties such as wound healing, analgesic, hemostatic, and antioxidant activities. Recently, the literature has shown scientific interest of its antimicrobial properties aiming the development of cost-effective phytotherapeutic agents. However, no literature are available in witch the antimicrobial and technological prospecting are summarized. AIM OF STUDY This study aimed to systematically review articles and patents related to the antimicrobial activity of B. orellana. METHODS The review followed the guidelines proposed by The Joanna Briggs Institute and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Two reviewers performed a literature search up to November 2021 in eight databases: Medline (PubMed), Scopus, Scifinder, Web of Science, Cochrane, Embase, Scielo, and Biblioteca Virtual em Saúde. The following databases for the patent search were analyzed: United States Patent and Trademark Office (USPTO), Google Patents, National Institute of Industrial Property (INPI), World Intellectual Property Organization (WIPO), and Espacenet (European Patent Office, EPO). The grey literature was searched using the ProQuest Dissertations and Periódicos Capes Theses database. The methodological quality and risk of bias in the included studies were carried out using Review Manager (RevMan) 5.3.5. RESULTS After analyzing the 47 studies and five patents fulfilled all the criteria and were included in the present investigation. The evidence suggests that this herbal medicine is effective against several fungi, Gram-positive and Gram-negative bacteria, being more effective to Gram-negative bacteria. Regarding the risk of bias and methodological quality analysis, most studies a high risk of bias related to random sequence generation and allocation concealment. CONCLUSION Up to now, the evidence in the literature suggests that the use of B. orellana preparations for antimicrobial preparations has some effectiveness. However, further research is needed using standard B. orellana preparations to determine their effectiveness as antimicrobial agents and expanding their application at an industrial level, in addition in vivo studies are needed for this confirmation.

Journal De Mycologie Medicale, Mar 1, 2018

This study aimed to evaluate the anti-enzymatic activity of Origanum vulgare (oregano) essential ... more This study aimed to evaluate the anti-enzymatic activity of Origanum vulgare (oregano) essential oil against 15 strains of Candida albicans. Candida albicans samples were isolated from the oral mucosa of patients with denture stomatitis treated in a Dentistry school on a public university. Preparation of the inoculum was performed with a suspension of C. albicans reactivated 24 h earlier in 5 mL of sterile phosphate buffer saline (PBS) adjusted to a 0.5turbidity on the MacFarland scale (1,5 Â 10 8 UFC/mL). The essential oil was obtained by hydrodistillation in a Clevenger-type machine and analyzed by gas chromatography. Enzymatic assay was performed to test phospholipase anti-enzymatic properties. Chromatography analysis revealed that the main compounds present in the essential oil were 4-terpineol (41.17%), thymol (21.95%), g-terpinene (5.91%) and carvacrol (4.71%). For the anti-enzymatic test, the statistical analysis showed that there was found statistically significant interactions between the factors time and concentration (P 0,001). Thus, essential oil of oregano at 1%, 5% and 10% presented significant reductions in the production of the phospholipase enzyme produced by Candida albicans strains. However, the longer the incubation time of the essential oil, there is a relatively moderate reduction in its anti-enzymatic activity.

Systematic Reviews, Jan 16, 2024

Systematic Reviews, Dec 13, 2023

Aging Clinical and Experimental Research

Revista Brasileira de Odontologia Legal - RBOL, 2018

Importância dos registros odontológicos e de radiografia panorâmica na identificação humana: um r... more Importância dos registros odontológicos e de radiografia panorâmica na identificação humana: um relato de caso.

Ciência, Tecnologia e Inovação: do campo à mesa, 2020

Resumo O leite é um produto altamente perecível e suas características físico-químicas e biológic... more Resumo O leite é um produto altamente perecível e suas características físico-químicas e biológicas se alteram facilmente com a ação de microrganismos. Casos de mastite e ma higienizacao no processo de ordenha posibilitam o aparecimento desses agentes. Como alternativa profilatica e terapêutica, as plantas medicinais ganham espaço na medicina veterinária como opção aos tratamentos e prevenções usuais. O objetivo deste estudo foi avaliar o potencial antibacteriano do extrato alcoólico de Jatropha dioica frente a bactérias isoladas de leite de vacas com mastite. A suscetibilidade de Staphylococcus spp. e Streptococcus spp., foi verificada através da técnica de Microdiluição em Caldo (CLSI, 2006). Observou-se inibição do crescimento dos isolados nas CIM de 28,12 µg mL-1 a 112,5 µg mL-1. Estes resultados sugerem potencial antimicrobiano do extrato alcoólico de J. dioica, caracterizando-a como promissora no tratamento e prevenção da mastite bovina e contaminação do leite.

Dansk Veterinaertidsskrift, 1980

Advances in human biology, 2020

Paediatric death inside dental office by the effect of anaesthesia in dentistry is a very difficu... more Paediatric death inside dental office by the effect of anaesthesia in dentistry is a very difficult case but not rare. In this article, we reviewed more than thirty studies with data focussing on death associated with dental anaesthesia using nitrous oxide (N2O). In children, most cases died at the age of 2–5 years. Hypoxia was the most common cause of death and cardiovascular, respiratory. Although rare death following general anaesthesia in dentistry, it is a critical side effect mostly seen in adult patients with compromised health conditions. Therefore, appropriate case selection in regard to patient's general health status as well as standard technical and equipment conditions is mandatory to reduce the risk of death during N2O dental anaesthesia in children. We have also given the list of guidelines and dietary care for children during N2O anaesthetic procedures inside the dental office.

Uma variedade de doencas envolve a cavidade oral, como a carie dental, considerada a patologia or... more Uma variedade de doencas envolve a cavidade oral, como a carie dental, considerada a patologia oral mais prevalente em todo o mundo. Atualmente, a carie dental e uma doenca multifatorial infecto-contagiosa, associada a bacterias patogenicas residentes do biofilme dental. Estes microrganismos sao os responsaveis pela producao de acidos e produtos citotoxicos que, respectivamente, levam a desmineralizacao de esmalte dental e inflamacao gengival com posterior destruicao dos tecidos de suporte dos dentes. Assim, o uso de agentes antimicrobianos eficientes contra essas bacterias e um importante meio de controle dessa doenca bucal. Varios agentes antimicrobianos de origem natural estao sendo investigados devido as suas possiveis propriedades farmacologicas. O cogumelo Agaricus brasiliensis S. Wasser et al. ( cogumelo-do-sol) destaca-se entre os produtos naturais com notaveis propriedades antibacterianas, antifungicas, antiinflamatorias e antitumorais. Neste estudo foi avaliado o efeito de tres extratos etanolicos brutos (EtOH 100%, 75% e 50%) deste cogumelo sobre o crescimento e a aderencia de estreptococos do grupo mutans. Os extratos etanolicos foram obtidos atraves do metodo de extracao por maceracao. Os controles negativos foram etanol 100%, 75% e 50%. Os testes antimicrobianos utilizados contra Streptococcus mutans UA 159 e Streptococcus sobrinus 6715 foram o metodo de difusao em agar, Concentracao Inibitoria Minima (CIM), Concentracao Bactericida Minima (CBM) e aderencia dos microrganismos a superficie de vidro (Adh). No metodo de difusao em agar, apenas o extrato 100% etanolico apresentou sinal de inibicao do crescimento microbiano. Ja nos testes de determinacao da CIM e CBM, os tres extratos apresentaram propriedades antimicrobianas e os valores de CIM e CBM variaram entre 87,4 e 444,5 μg/mL. A CBM para os tres extratos etanolicos foi igual a CIM para S.sobrinus, mas apenas o extrato etanolico 75% foi bactericida para S. mutans. Entre os extratos etanolicos testados, o extrato 100% etanolico foi o mais ativo (CIM 87.4μg/mL). A aderencia celular tambem foi inibida em concentracoes de 100 a 400μg/mL dos extratos obtidos com etanol 50% e 75%. Concluindo, os extratos etanolicos de A. brasiliensis mostraram notaveis efeitos inibitorios no crescimento bacteriano e o extrato 100% etanolico foi o mais ativo

Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2024

Objective: To review the literature unprecedentedly to identify the dentistry students' knowledge... more Objective: To review the literature unprecedentedly to identify the dentistry students' knowledge of pediatric patients who suffered violence. Material and Methods: A systematic review was conducted using PubMed, Web of Science, LILACS, SciELO, Google Scholar, and OpenGrey electronic databases up to November 2021. The "Grey Literature" was verified through Google Scholar and Open Grey searches to avoid any selection bias. There was no restriction on the date of publication or language. Results: The systematic search yielded 2.756 studies in the first selection phase, but only twenty-two articles were included. All selected articles were published between 1998 and 2021 and used a questionnaire to evaluate the dentistry students' knowledge regarding child maltreatment cases. Brazil was the country that had the most studies included (10 articles). Despite the majority of the students presenting insufficient knowledge about child maltreatment, evidence from this research showed that every form of approach by professionals toward child maltreatment should be considered important. Conclusion: Therefore, there are deficiencies regarding the teaching-learning methodology, reinforcing the need for improvements in Dentistry undergraduate curricula.

Brazilian Journal of Oral Sciences, Nov 29, 2023

Aim: This study aimed to investigate the prevalence of physical violence and head and neck injuri... more Aim: This study aimed to investigate the prevalence of physical violence and head and neck injuries among children and adolescents who suffered physical aggression, firearm aggression, and white gun aggression referred to the Legal Medical Department in a southern city in Brazil. Methods: This study was performed at Legal Medical Department in the city of Pelotas, RS, Brazil, from January 2011 to December of 2015. Data were collected from medical reports of children and adolescents (0-19 years old). Demographic information such as sex, age, and skin color was collected. Data were submitted to Chi-square test and multivariate Poisson regression analysis. Results: In this study, 2,716 physical examination reports were analyzed, with a total of 2,171 exams resulting from violence; lesions that had physical aggression as their etiology (1,951) had a higher prevalence. The analysis of the exams revealed that the majority were adolescents (90,05%), white (84.09%), and female (50.21%). The head and neck regions were affected in most cases of violence (57.90%). In the adjusted model, the occurrence of injuries in the head and neck region was higher in males (PR 1.16, CI 95% 1.08-1.25), among adolescents (PR 1.28, CI 95% 1.10-1.48) and in victims of physical aggression compared to victims of the firearm (2.81, CI 95% 1.79-4.40). Conclusion: The results revealed a high prevalence of head and face injuries in victims of violence and that there was a greater prevalence of physical violence among adolescents compared to children and males.

Revista Brasileira de Odontologia Legal - RBOL, Dec 6, 2020

Avaliação de danos pessoais em odontologia e discussão sobre seguro DPVATrelato de caso.

Revista da ABENO, Oct 31, 2020

Research Square (Research Square), Sep 28, 2022

This systematic review covers the existing evidence in the literature regarding the association o... more This systematic review covers the existing evidence in the literature regarding the association of propolis with controlled delivery systems (DDS) and its potential therapeutic action in dental medicine. This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Two independent reviewers performed a literature search up to 15th January 2022 in ve databases: PubMed/Medline, Web of Science, Cochrane Library, Scopus, and Embase, to identify the eligible studies. Clinical, in situ, and in vitro studies that investigated the incorporation of propolis as the main agent in DDS for dental medicine were included in this study. Conversly, review articles, clinical cases, theses, dissertations, abstracts at congresses, and studies that had no application in dentistry were excluded. After examining carefully 55 full-text articles, 11 in vitro studies, 1 animal model, and 1 in vivo and in vitro study were included (n = 13). Relevant data were extracted from the included studies and analyzed qualitatively. The use of propolis has been reported in cariology, endodontics periodontics, and implantology. Propolis has shown non-cytotoxic, osteoinductive, antimicrobial, and anti-in ammatory properties. Moreover, propolis can readly be released from DDS for prolonged periods, having potential biocompatibility, safety, and potential advantage for applications in dental medicine.

Journal of Prosthetic Dentistry, Apr 1, 2021

STATEMENT OF PROBLEM The high recurrence rates of denture stomatitis may be associated with the r... more STATEMENT OF PROBLEM The high recurrence rates of denture stomatitis may be associated with the resistance of biofilms to therapeutics. Therefore, methods that provide biomaterials with antifungal properties are an attractive solution to improving microbial control. PURPOSE The purpose of this in vitro study was to modify conventional polymethyl methacrylate (PMMA) through the incorporation of metal methacrylate monomers and to evaluate the physicomechanical and optical properties and antifungal activity of the modified materials. MATERIAL AND METHODS Experimental denture base acrylic resins were fabricated through the addition of zirconium methacrylate (ZM), tin methacrylate (TM), and di-n-butyldimethacrylate-tin (DNBMT) to the liquid of a commercially available denture base PMMA resin. Unmodified PMMA resin was used as the control. The degree of conversion of the materials was tested through Fourier transform infrared spectroscopy (n=3). A digital spectrophotometer was used to assess the color change of the modified materials (n=8). Differences in Knoop hardness and roughness between experimental groups were also evaluated (n=8). A biofilm accumulation test with Candida albicans (ATCC 62342) (n=4) was performed for 5 days in Sabouraud broth culture supplemented with 10% sucrose. Data were subjected to analysis of variance and the post hoc Tukey honestly significant difference test (α=.05). RESULTS The degree of conversion and color-change values of the experimental materials were statistically similar to those of the control (P=.593). The incorporation of DNBMT significantly increased the hardness of the modified material (P=.014). The ZM, TM, and DNBMT groups had higher antifungal activity against C. albicans (P=.001) and lower roughness than the control group (control 0.65 ±0.05 μm; ZM 0.34 ±0.09 μm, TM 0.34 ±0.11 μm, and DNBMT 0.41 ±0.08 μm). CONCLUSIONS The metal-containing methacrylate monomers provided antifungal action to the modified materials without affecting the physicomechanical or optical properties of the denture base resin. ZM, TM, and DNBMT are potential reactive agents for the fabrication of PMMA denture base resins with antifungal properties.

Biofouling, Apr 20, 2020

The objective of this study was to evaluate the physico-chemical and antimicrobial properties of ... more The objective of this study was to evaluate the physico-chemical and antimicrobial properties of a dual polymerization experimental endodontic sealer (E) and experimental sealers containing dibutyltin methacrylate (Sn2+) (ETs) or calcium methacrylate (Ca2+) (ECs). The pH and ion release levels of the sealers were measured. The dimensional stability was evaluated in accordance with ISO 6876. Biofilm growth inhibition was evaluated using confocal laser scanning microscopy (CLSM). Biofilm viability analysis was performed using the SYTO 9 technique. The shelf life was evaluated through the degree of conversion and film thickness tests after the sealers had been stored for different periods of time. For statistical analysis, ANOVA and Tukey's post hoc test were used, with a significance level of 5%. ETs revealed better anti-biofilm potential after 15 days than that of the controls. The degree of conversion was reduced after the shelf-life period. The addition of calcium and dibutyltin methacrylate improved the anti-biofilm properties of the experimental endodontic sealer without impairing their physico-chemical properties.

Journal of Ethnopharmacology, Apr 1, 2022

ETHNOPHARMACOLOGICAL RELEVANCE Bixa orellana L. is reported to have numerous applications in trad... more ETHNOPHARMACOLOGICAL RELEVANCE Bixa orellana L. is reported to have numerous applications in traditional medicine and pharmacological properties such as wound healing, analgesic, hemostatic, and antioxidant activities. Recently, the literature has shown scientific interest of its antimicrobial properties aiming the development of cost-effective phytotherapeutic agents. However, no literature are available in witch the antimicrobial and technological prospecting are summarized. AIM OF STUDY This study aimed to systematically review articles and patents related to the antimicrobial activity of B. orellana. METHODS The review followed the guidelines proposed by The Joanna Briggs Institute and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Two reviewers performed a literature search up to November 2021 in eight databases: Medline (PubMed), Scopus, Scifinder, Web of Science, Cochrane, Embase, Scielo, and Biblioteca Virtual em Saúde. The following databases for the patent search were analyzed: United States Patent and Trademark Office (USPTO), Google Patents, National Institute of Industrial Property (INPI), World Intellectual Property Organization (WIPO), and Espacenet (European Patent Office, EPO). The grey literature was searched using the ProQuest Dissertations and Periódicos Capes Theses database. The methodological quality and risk of bias in the included studies were carried out using Review Manager (RevMan) 5.3.5. RESULTS After analyzing the 47 studies and five patents fulfilled all the criteria and were included in the present investigation. The evidence suggests that this herbal medicine is effective against several fungi, Gram-positive and Gram-negative bacteria, being more effective to Gram-negative bacteria. Regarding the risk of bias and methodological quality analysis, most studies a high risk of bias related to random sequence generation and allocation concealment. CONCLUSION Up to now, the evidence in the literature suggests that the use of B. orellana preparations for antimicrobial preparations has some effectiveness. However, further research is needed using standard B. orellana preparations to determine their effectiveness as antimicrobial agents and expanding their application at an industrial level, in addition in vivo studies are needed for this confirmation.

Journal De Mycologie Medicale, Mar 1, 2018

This study aimed to evaluate the anti-enzymatic activity of Origanum vulgare (oregano) essential ... more This study aimed to evaluate the anti-enzymatic activity of Origanum vulgare (oregano) essential oil against 15 strains of Candida albicans. Candida albicans samples were isolated from the oral mucosa of patients with denture stomatitis treated in a Dentistry school on a public university. Preparation of the inoculum was performed with a suspension of C. albicans reactivated 24 h earlier in 5 mL of sterile phosphate buffer saline (PBS) adjusted to a 0.5turbidity on the MacFarland scale (1,5 Â 10 8 UFC/mL). The essential oil was obtained by hydrodistillation in a Clevenger-type machine and analyzed by gas chromatography. Enzymatic assay was performed to test phospholipase anti-enzymatic properties. Chromatography analysis revealed that the main compounds present in the essential oil were 4-terpineol (41.17%), thymol (21.95%), g-terpinene (5.91%) and carvacrol (4.71%). For the anti-enzymatic test, the statistical analysis showed that there was found statistically significant interactions between the factors time and concentration (P 0,001). Thus, essential oil of oregano at 1%, 5% and 10% presented significant reductions in the production of the phospholipase enzyme produced by Candida albicans strains. However, the longer the incubation time of the essential oil, there is a relatively moderate reduction in its anti-enzymatic activity.

Systematic Reviews, Jan 16, 2024

Systematic Reviews, Dec 13, 2023

Aging Clinical and Experimental Research

Revista Brasileira de Odontologia Legal - RBOL, 2018

Importância dos registros odontológicos e de radiografia panorâmica na identificação humana: um r... more Importância dos registros odontológicos e de radiografia panorâmica na identificação humana: um relato de caso.