Amanda Cristina Schoeffel | Universidade Federal do Paraná (original) (raw)

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Papers by Amanda Cristina Schoeffel

Current Drug Delivery

Background: Nystatin (Nys) is a fungicidal drug commonly prescribed for candidiasis disease in se... more Background: Nystatin (Nys) is a fungicidal drug commonly prescribed for candidiasis disease in several administration routes. However, Nys is a class IV drug according to the Biopharmaceutical Classification System, that possesses limited bioavailability, and is used for local activity. Objective: This study developed and characterized nystatin:β-cyclodextrin (Nys:βCD) inclusion complexes and evaluated their activity against Candida spp. Methods: Complexes were characterized by physicochemical techniques and drug dissolution profiles. The susceptibility of C. albicans, C. krusei, C. parapsilosis, C. glabrata, C. guilliermondii, C. tropicalis, and C. auris was assessed using the broth microdilution method. The applicability of Nys:βCD inclusion complex was evaluated by incorporating it into a temporary soft material for denture stomatitis treatment Results: Nys was better complexed in a 1:1 molar ratio by freeze-drying and spray-drying methods. The inclusion complexes show bi-exponen...

Indian Journal of Dental Research, 2019

Background: Microwave polymerization reduces the time of acrylic resin processing, but it is impo... more Background: Microwave polymerization reduces the time of acrylic resin processing, but it is important to select a proper cycle to avoid overheating, porosity, and impairment to the materials' properties. Aims: To evaluate the microhardness of conventional (Vipi Cril-VC) and microwaved (Vipi Wave-VW) acrylic resins after microwave polymerization cycles and the cycles recommended by the manufacturer. It was also evaluated the effect of thermocycling on the microhardness of these materials. Methods and Materials: Specimens (n = 10) were made: 1. WB (water-bath recommended for VC polymerization); 2. M630/25 (10 min at 270 W; 5 min at 0 W; 10 min at 360 W: recommended for VW polymerization); 3. M550/3 (3 min at 550 W); and 4. M650/5 (5 min at 650 W). After 48 h in distilled water at 37°C, specimens were subjected to Knoop test under 25 g during 5 s. The same specimens were submitted to thermocycling (5,000 cycles; 5°C and 55°C; 60 s) and the microhardness was again measured. The results were analyzed by repeated measures one-way analysis of variance/Bonferroni for each material (α = 0.05). Results: For both materials, no difference was obtained for groups polymerized using the cycles recommended by the manufacturer (VC = 19.8 ± 0.9 KHN; VW = 21.0 ± 1.6 KHN) and M650/5 (VC = 19.9 ± 1.5 KHN; VW = 20.9 ± 0.8 KHN). After thermocycling, microhardness decreased in experimental cycles for VC (M550/3 = 17.0 ± 1.9 to 14.6 ± 1.2 KHN; M650/5 = 19.9 ± 1.5 to 17.8 ± 0.8 KHN) and in all microwave cycles for VW (M630/25 = 21.0 ± 1.6 to 18.3 ± 0.9 KHN; M550/3 = 17.5 ± 3.0 to 15.7 ± 2.0 KHN; M650/5 = 20.9 ± 0.8 to 18.2 ± 2.0 KHN) (P = 0.000). The lowest hardness was observed for both resins processed by M550/3 (P = 0.001). Conclusions: Both materials could be polymerized in microwave during 5 min showing hardness similar to the cycles recommended by the manufacturer. Thermocycling did not decrease the hardness of VC polymerized with both cycles recommended by the manufacturer and VW polymerized with the water-bath conventional cycle.

Orientadora : Profa . Dra . Juliana Feltrin de SouzaMonografia (especialização) - Universidade Fe... more Orientadora : Profa . Dra . Juliana Feltrin de SouzaMonografia (especialização) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Curso de Especialização em OdontopediatriaInclui referênciasResumo : O bruxismo do sono (BS) é considerado uma atividade involuntária do sistema mastigatório, caracterizado pelo ato de ranger ou apertar os dentes ou por forçadamente manter ou movimentar a mandíbula, devido à atividade muscular repetitiva, o que pode trazer consequências negativas para o sistema estomatognático, bem como para a qualidade de vida do indivíduo. O BS em crianças tem sido frequentemente reportado pelos pais, sendo motivo de grande preocupação em pais e cuidadores devido ao barulho produzido, o qual pode ser intenso e repetitivo por um período prolongado de tempo. A etiologia do BS durante a infância não é completamente conhecida, sendo considerada multifatorial, e podendo envolver fatores locais, sistêmicos e psicológicos. A ansiedade está entre os fatores emociona...

RSBO

Os odontomas são os tumores benignos mais comuns, sendo considerados tumores mistos em virtude de... more Os odontomas são os tumores benignos mais comuns, sendo considerados tumores mistos em virtude de sua origem epitelial e ectomesenquimal. Objetivo: Relatar o diagnóstico e tratamento de um caso de remoção cirúrgica sob anestesia geral de odontoma composto em um paciente com transtorno do espectro autista (TEA). Relato de caso: Paciente do gênero masculino, portador de TEA, assintomático, queixa principal de ausência de irrupção do dente 31. Após exame de imagem constatou-se presença de uma lesão com conteúdo semelhante a múltiplos dentículos, sugerindo diagnóstico de odontoma composto. Levando em consideração a idade do paciente e a condição apresentada (TEA), a criança foi encaminhada para cirurgia em âmbito hospitalar, sob anestesia geral. Foi realizada exérese cirúrgica da lesão, e após análise histopatológica se confirmou diagnóstico inicial de odontoma composto. Conclusão: O diagnóstico precoce permite a adoção de tratamento menos complexo, o que permite melhor prognóstico. Alé...

Materials Chemistry and Physics, 2019

Abstract The inclusion complex of Nystatin (NYS) with β -cyclodextrin ( β -CD) can increase the s... more Abstract The inclusion complex of Nystatin (NYS) with β -cyclodextrin ( β -CD) can increase the solubility of the antibiotic in aqueous media. The complexes were obtained by freeze-drying (FD) and spray-drying (SD) methods. The spectroscopic techniques used to analyze the complexes were FT-Raman, photoacoustic spectroscopy (PAS), and 1H NMR. FT-Raman allowed the identification of the interactions of β -CD with NYS molecule through the vibrational modes assigned to tetraene, heptaene and C=O. PAS confirms that the main part of interaction from NYS is heptaene. The 1H NMR showed that the hydrogens inside the cavity of β -CD interact with NYS. These techniques confirmed the complex formation and that the drying method affects the form of complexation: by the FD method one molecule of β -CD is sufficient to form the complex with NYS. In contrast, the inclusion complex of NYS: β -CD by the SD method is formed by two molecules of β -CD. These results showed the effectiveness of these techniques in evaluating the inclusion complex and demonstrate that the drying method affects how this complexation occurs.

Objetivo: A proposta deste estudo foi elaborar complexos de inclusao (CI) entre clorexidina (Clx)... more Objetivo: A proposta deste estudo foi elaborar complexos de inclusao (CI) entre clorexidina (Clx) e β-ciclodextrina (βCD) para a posterior incorporacao em material condicionador de tecido, visando promover a liberacao controlada e permitir um efeito antifungico prolongado. Material e metodos: Suspensoes de Clx:βCD em proporcoes molares de 1:0,125, 1:0,25, 1:0,5, 1:0,625, 1:1, 1:2 e 1:4 foram preparadas em triplicata em agua destilada, agitadas por 24 h e avaliadas com relacao a solubilidade de fases. As proporcoes 1:1 e 1:2 foram selecionadas para obtencao de CI a partir dos seguintes metodos: mistura fisica (MF), liofilizacao (Lio) e spray drying (Spray). A MF foi homogeneizada por 15 min com almofariz e pistilo. Os demais CI foram dissolvidos em solucao de agua/etanol (50:50 v/v), agitados por 24 h e, entao, submetidos a um dos processos de secagem Lio ou Spray. Clx, βCD e CI foram submetidos as seguintes caracterizacoes fisico-quimicas: microscopia eletronica de varredura por emi...

Dental Materials Journal, 2015

The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or mi... more The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or microwave-processed (Vipi Wave ®-VW) denture base acrylic resins was evaluated. Specimens (n=10) were submitted to the cycles: WB=65ºC for 1 h+1 h boiling water (VC cycle); M630/25=10 min at 270 W+5 min at 0 W+10 min at 360 W (VW cycle); M650/5=5 min at 650 W; M700/4=4 min at 700 W; and M550/3=3 min at 550 W. Specimens were submitted to a three-point bending test at 5 mm/min until fracture. Flexural strength (MPa) and elastic modulus (GPa) data were analyzed by 2-way ANOVA/Tukey HSD (α=0.05). Overall, VC showed higher values than VW. The results obtained with microwave polymerization did not differ from those obtained with water-bath for both acrylic resins. The results observed when polymerization cycles using medium power and shorter time were used did not differ from those when manufacturer's recommended microwave cycle was applied. Conventional VC might be microwave-processed without compromising its flexural properties.

Dental Materials Journal, 2015

The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or mi... more The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or microwave-processed (Vipi Wave ®-VW) denture base acrylic resins was evaluated. Specimens (n=10) were submitted to the cycles: WB=65ºC for 1 h+1 h boiling water (VC cycle); M630/25=10 min at 270 W+5 min at 0 W+10 min at 360 W (VW cycle); M650/5=5 min at 650 W; M700/4=4 min at 700 W; and M550/3=3 min at 550 W. Specimens were submitted to a three-point bending test at 5 mm/min until fracture. Flexural strength (MPa) and elastic modulus (GPa) data were analyzed by 2-way ANOVA/Tukey HSD (α=0.05). Overall, VC showed higher values than VW. The results obtained with microwave polymerization did not differ from those obtained with water-bath for both acrylic resins. The results observed when polymerization cycles using medium power and shorter time were used did not differ from those when manufacturer's recommended microwave cycle was applied. Conventional VC might be microwave-processed without compromising its flexural properties.

Current Drug Delivery

Background: Nystatin (Nys) is a fungicidal drug commonly prescribed for candidiasis disease in se... more Background: Nystatin (Nys) is a fungicidal drug commonly prescribed for candidiasis disease in several administration routes. However, Nys is a class IV drug according to the Biopharmaceutical Classification System, that possesses limited bioavailability, and is used for local activity. Objective: This study developed and characterized nystatin:β-cyclodextrin (Nys:βCD) inclusion complexes and evaluated their activity against Candida spp. Methods: Complexes were characterized by physicochemical techniques and drug dissolution profiles. The susceptibility of C. albicans, C. krusei, C. parapsilosis, C. glabrata, C. guilliermondii, C. tropicalis, and C. auris was assessed using the broth microdilution method. The applicability of Nys:βCD inclusion complex was evaluated by incorporating it into a temporary soft material for denture stomatitis treatment Results: Nys was better complexed in a 1:1 molar ratio by freeze-drying and spray-drying methods. The inclusion complexes show bi-exponen...

Indian Journal of Dental Research, 2019

Background: Microwave polymerization reduces the time of acrylic resin processing, but it is impo... more Background: Microwave polymerization reduces the time of acrylic resin processing, but it is important to select a proper cycle to avoid overheating, porosity, and impairment to the materials' properties. Aims: To evaluate the microhardness of conventional (Vipi Cril-VC) and microwaved (Vipi Wave-VW) acrylic resins after microwave polymerization cycles and the cycles recommended by the manufacturer. It was also evaluated the effect of thermocycling on the microhardness of these materials. Methods and Materials: Specimens (n = 10) were made: 1. WB (water-bath recommended for VC polymerization); 2. M630/25 (10 min at 270 W; 5 min at 0 W; 10 min at 360 W: recommended for VW polymerization); 3. M550/3 (3 min at 550 W); and 4. M650/5 (5 min at 650 W). After 48 h in distilled water at 37°C, specimens were subjected to Knoop test under 25 g during 5 s. The same specimens were submitted to thermocycling (5,000 cycles; 5°C and 55°C; 60 s) and the microhardness was again measured. The results were analyzed by repeated measures one-way analysis of variance/Bonferroni for each material (α = 0.05). Results: For both materials, no difference was obtained for groups polymerized using the cycles recommended by the manufacturer (VC = 19.8 ± 0.9 KHN; VW = 21.0 ± 1.6 KHN) and M650/5 (VC = 19.9 ± 1.5 KHN; VW = 20.9 ± 0.8 KHN). After thermocycling, microhardness decreased in experimental cycles for VC (M550/3 = 17.0 ± 1.9 to 14.6 ± 1.2 KHN; M650/5 = 19.9 ± 1.5 to 17.8 ± 0.8 KHN) and in all microwave cycles for VW (M630/25 = 21.0 ± 1.6 to 18.3 ± 0.9 KHN; M550/3 = 17.5 ± 3.0 to 15.7 ± 2.0 KHN; M650/5 = 20.9 ± 0.8 to 18.2 ± 2.0 KHN) (P = 0.000). The lowest hardness was observed for both resins processed by M550/3 (P = 0.001). Conclusions: Both materials could be polymerized in microwave during 5 min showing hardness similar to the cycles recommended by the manufacturer. Thermocycling did not decrease the hardness of VC polymerized with both cycles recommended by the manufacturer and VW polymerized with the water-bath conventional cycle.

Orientadora : Profa . Dra . Juliana Feltrin de SouzaMonografia (especialização) - Universidade Fe... more Orientadora : Profa . Dra . Juliana Feltrin de SouzaMonografia (especialização) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Curso de Especialização em OdontopediatriaInclui referênciasResumo : O bruxismo do sono (BS) é considerado uma atividade involuntária do sistema mastigatório, caracterizado pelo ato de ranger ou apertar os dentes ou por forçadamente manter ou movimentar a mandíbula, devido à atividade muscular repetitiva, o que pode trazer consequências negativas para o sistema estomatognático, bem como para a qualidade de vida do indivíduo. O BS em crianças tem sido frequentemente reportado pelos pais, sendo motivo de grande preocupação em pais e cuidadores devido ao barulho produzido, o qual pode ser intenso e repetitivo por um período prolongado de tempo. A etiologia do BS durante a infância não é completamente conhecida, sendo considerada multifatorial, e podendo envolver fatores locais, sistêmicos e psicológicos. A ansiedade está entre os fatores emociona...

RSBO

Os odontomas são os tumores benignos mais comuns, sendo considerados tumores mistos em virtude de... more Os odontomas são os tumores benignos mais comuns, sendo considerados tumores mistos em virtude de sua origem epitelial e ectomesenquimal. Objetivo: Relatar o diagnóstico e tratamento de um caso de remoção cirúrgica sob anestesia geral de odontoma composto em um paciente com transtorno do espectro autista (TEA). Relato de caso: Paciente do gênero masculino, portador de TEA, assintomático, queixa principal de ausência de irrupção do dente 31. Após exame de imagem constatou-se presença de uma lesão com conteúdo semelhante a múltiplos dentículos, sugerindo diagnóstico de odontoma composto. Levando em consideração a idade do paciente e a condição apresentada (TEA), a criança foi encaminhada para cirurgia em âmbito hospitalar, sob anestesia geral. Foi realizada exérese cirúrgica da lesão, e após análise histopatológica se confirmou diagnóstico inicial de odontoma composto. Conclusão: O diagnóstico precoce permite a adoção de tratamento menos complexo, o que permite melhor prognóstico. Alé...

Materials Chemistry and Physics, 2019

Abstract The inclusion complex of Nystatin (NYS) with β -cyclodextrin ( β -CD) can increase the s... more Abstract The inclusion complex of Nystatin (NYS) with β -cyclodextrin ( β -CD) can increase the solubility of the antibiotic in aqueous media. The complexes were obtained by freeze-drying (FD) and spray-drying (SD) methods. The spectroscopic techniques used to analyze the complexes were FT-Raman, photoacoustic spectroscopy (PAS), and 1H NMR. FT-Raman allowed the identification of the interactions of β -CD with NYS molecule through the vibrational modes assigned to tetraene, heptaene and C=O. PAS confirms that the main part of interaction from NYS is heptaene. The 1H NMR showed that the hydrogens inside the cavity of β -CD interact with NYS. These techniques confirmed the complex formation and that the drying method affects the form of complexation: by the FD method one molecule of β -CD is sufficient to form the complex with NYS. In contrast, the inclusion complex of NYS: β -CD by the SD method is formed by two molecules of β -CD. These results showed the effectiveness of these techniques in evaluating the inclusion complex and demonstrate that the drying method affects how this complexation occurs.

Objetivo: A proposta deste estudo foi elaborar complexos de inclusao (CI) entre clorexidina (Clx)... more Objetivo: A proposta deste estudo foi elaborar complexos de inclusao (CI) entre clorexidina (Clx) e β-ciclodextrina (βCD) para a posterior incorporacao em material condicionador de tecido, visando promover a liberacao controlada e permitir um efeito antifungico prolongado. Material e metodos: Suspensoes de Clx:βCD em proporcoes molares de 1:0,125, 1:0,25, 1:0,5, 1:0,625, 1:1, 1:2 e 1:4 foram preparadas em triplicata em agua destilada, agitadas por 24 h e avaliadas com relacao a solubilidade de fases. As proporcoes 1:1 e 1:2 foram selecionadas para obtencao de CI a partir dos seguintes metodos: mistura fisica (MF), liofilizacao (Lio) e spray drying (Spray). A MF foi homogeneizada por 15 min com almofariz e pistilo. Os demais CI foram dissolvidos em solucao de agua/etanol (50:50 v/v), agitados por 24 h e, entao, submetidos a um dos processos de secagem Lio ou Spray. Clx, βCD e CI foram submetidos as seguintes caracterizacoes fisico-quimicas: microscopia eletronica de varredura por emi...

Dental Materials Journal, 2015

The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or mi... more The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or microwave-processed (Vipi Wave ®-VW) denture base acrylic resins was evaluated. Specimens (n=10) were submitted to the cycles: WB=65ºC for 1 h+1 h boiling water (VC cycle); M630/25=10 min at 270 W+5 min at 0 W+10 min at 360 W (VW cycle); M650/5=5 min at 650 W; M700/4=4 min at 700 W; and M550/3=3 min at 550 W. Specimens were submitted to a three-point bending test at 5 mm/min until fracture. Flexural strength (MPa) and elastic modulus (GPa) data were analyzed by 2-way ANOVA/Tukey HSD (α=0.05). Overall, VC showed higher values than VW. The results obtained with microwave polymerization did not differ from those obtained with water-bath for both acrylic resins. The results observed when polymerization cycles using medium power and shorter time were used did not differ from those when manufacturer's recommended microwave cycle was applied. Conventional VC might be microwave-processed without compromising its flexural properties.

Dental Materials Journal, 2015

The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or mi... more The effect of polymerization cycles on flexural properties of conventional (Vipi Cril ®-VC) or microwave-processed (Vipi Wave ®-VW) denture base acrylic resins was evaluated. Specimens (n=10) were submitted to the cycles: WB=65ºC for 1 h+1 h boiling water (VC cycle); M630/25=10 min at 270 W+5 min at 0 W+10 min at 360 W (VW cycle); M650/5=5 min at 650 W; M700/4=4 min at 700 W; and M550/3=3 min at 550 W. Specimens were submitted to a three-point bending test at 5 mm/min until fracture. Flexural strength (MPa) and elastic modulus (GPa) data were analyzed by 2-way ANOVA/Tukey HSD (α=0.05). Overall, VC showed higher values than VW. The results obtained with microwave polymerization did not differ from those obtained with water-bath for both acrylic resins. The results observed when polymerization cycles using medium power and shorter time were used did not differ from those when manufacturer's recommended microwave cycle was applied. Conventional VC might be microwave-processed without compromising its flexural properties.