Matheus Viezzer Bianchi | Universidade Federal do Rio Grande do Sul (original) (raw)

Papers by Matheus Viezzer Bianchi

Tropical Animal Health and Production

Ciência Rural

ABSTRACT: Bee envenomation is frequent in humans and dogs, but uncommon in horses. This study aim... more ABSTRACT: Bee envenomation is frequent in humans and dogs, but uncommon in horses. This study aimed to describe a case of acute renal failure following mass envenomation in a horse. A mare was attacked by a swarm of bees and showed reluctance to move, epistaxis, and dark-brown urine. Biochemical exams revealed increase in urea and creatine serum levels. The mare did not respond to treatment and euthanasia was elected after four days of clinical course. At the necropsy, there were multifocal pinpoint to elevated skin lesions associated with edema and hemorrhage, which extended to the subcutaneous tissue and skeletal muscle, and the kidneys were diffusely dark-brown and friable. Microscopically, renal tubules were distended and filled with an orange-red, hyaline globular material, and had severe epithelial tubular cell necrosis. The diagnosis was established based on clinical and histological analysis, and pathological evaluation was essential to confirm acute renal failure due to bee...

Medical Mycology Case Reports

Ciência Rural

ABSTRACT: Meningiomas are the most frequent neoplasms involving the brain in dogs and cats, and a... more ABSTRACT: Meningiomas are the most frequent neoplasms involving the brain in dogs and cats, and are occasionally observed in the spinal cord. They cause compression of the central nervous system; however, do not infiltrate the neuropile and rarely metastasize to other organs. The present study describes a case of anaplastic spinal meningioma with extra neural metastasis in a 20 years-old cat. Clinically, the feline presented a clinical history of 120 days with paresis of the hind limbs and loss of the tail’s movements, with subsequent death. At necropsy, there was an irregular and soft whitish mass involving the meninges from the lumbar intumescence to the sacral region of the spinal cord. Similar white nodular masses were observed in the lungs, liver and kidneys. Microscopically, both were composed of a poorly limited and infiltrative neoplastic proliferation composed by spindle, round and epithelioid cells, with a high cellular pleomorphism. On IHC, there was a severe immunostaini...

Acta Scientiae Veterinariae

Background: Cutaneous lesions in horses are common, accounting for approximately 50% of all neopl... more Background: Cutaneous lesions in horses are common, accounting for approximately 50% of all neoplasms of this species, and can cause irritability, pruritus and predispose to secondary infections. Studies have provided information of frequency of cutaneous neoplasms in horses, but few included non-neoplastic lesions. The aim of this study is to identify the frequency of cutaneous lesions in horses diagnosed in Southern Brazil, through a retrospective study and identification of the anatomic location of the lesion, the breed, gender and age of the animals affected.Materials, Methods & Results: A retrospective study on skin lesions in horses using the database of the Veterinary Pathology Laboratory located in Southern Brazil was carried out from January 2000 to February 2013. Cases were searched for and selected by diagnosis, considering age, breed, gender, location and time of occurrence. The most common lesions included sarcoid, exuberant granulation tissue, squamous cell carcinoma (...

SummaryMosquito borne flaviviruses cause a series of important diseases in humans and animals. Th... more SummaryMosquito borne flaviviruses cause a series of important diseases in humans and animals. These viruses are maintained in cycles involving replication in mosquito and in vertebrate hosts. Most natural hosts are vertebrate animals living in sylvatic or peridomestic environments. Human contact with these environments may result in host shifts that lead to the establishment of urban transmission cycles. Zika virus is a Flavivirus that persists in nature in a transmission cycle involving non-human primates (NHP). Its recent emergence in Brazil has shed light upon the importance of surveying this agent in Brazilian sylvatic environments. Here we present histopathological and molecular evidence that free ranging howler monkeys (Alouatta guariba) in Southern Brazil are infected by ZIKV closely related to African lineage MR766. Nine NHP were nested RT-PCR positive for ZIKV RNA. Sequence analysis revealed 96 to 98% identity to ZIKV MR766 and 85% identity to ZIKV P6-740, the current epid...

Journal of Veterinary Science

A 3-year-old mixed-breed female cat was diagnosed with a ventricular septal defect of the heart t... more A 3-year-old mixed-breed female cat was diagnosed with a ventricular septal defect of the heart through an echocardiogram. After a 9-month treatment, progressive and diffuse hard thickening of all limbs was observed, which on radiographic examinations, revealed a marked thickening of the long bones. The necropsy findings were limited to the appendicular skeleton and thoracic vertebrae, in addition to a severe cardiac interventricular septal defect and lung edema. The histological evaluation revealed severe replacement of the cortical bone by spongy bone in all bone fragments examined. This is the first report of hypertrophic osteopathy occurring in association with a cardiac malformation in a cat.

Ciência Rural

ABSTRACT: Meningiomas are neoplasms that commonly involve the central nervous system of cats, whi... more ABSTRACT: Meningiomas are neoplasms that commonly involve the central nervous system of cats, while cholesteatomas are nodular granulomatous chronic lesions within the choroid plexus that are rarely reported in cats. This study described a case of cholesteatoma and non-communicating hydrocephalus associated to a third ventricle meningioma in a cat. Clinically, the cat had a 2-year history of behavioral changes, photophobia and motor incoordination. At the necropsy, a tan-brown mass totally occluded the third ventricle, causing a severe dilation of the lateral ventricles (non-communicating hydrocephalus). Microscopically, the mass was composed by a neoplastic proliferation of spindle cells arranged in bundles, containing in the center psammomatous bodies (meningioma), while in the adjacent areas a cholesteatoma was observed, which was characterized by multiple cholesterol cleft formation, hemosiderosis and associated granulomatous inflammation. At immunohistochemistry (IHC), neoplast...

Revista Brasileira de Parasitologia Veterinária

Diseases related to the alimentary system are the main cause of death in horses. This retrospecti... more Diseases related to the alimentary system are the main cause of death in horses. This retrospective study aimed to describe the pathological findings of fatal parasite-induced enteritis and typhlocolitis caused by cyathostominae, Eimeria leuckarti, Balantidium coli, and Strongyloides westeri in horses. The records of parasite-induced intestinal lesions in horses necropsied in Southern Brazil between 2005 and 2017 were reviewed. Ten horses had fatal parasitic enteritis and/or typhlocolitis, and the main causes were: cyathostominae typhlocolitis (6/10), E. leuckarti enteritis (1/10), S. westeri enteritis (1/10), B. coli colitis related to cyathostominae (1/10), and infection by multiple agents (1/10). Cyathostominae typhlocolitis showed marked mucosal thickening, with multifocal elevated nodules containing tangled filiform parasites. Microscopic examination revealed that the mucosa and submucosa had encysted parasitic structures surrounded by eosinophilic and granulomatous inflammatio...

Acta Scientiae Veterinariae

Background: Ricinus communis (castor bean) is an arbustive plant, popularly known in Brazil as “m... more Background: Ricinus communis (castor bean) is an arbustive plant, popularly known in Brazil as “mamona”. This plant is widely distributed both as a weed in pastures and in specific cultures. All parts of the plant are poisonous and contain the toxic substance ricin, especially the seeds, leaves and pericarp. The ingestion of leaves and pericarp may cause neurological signs. Gross findings are unspecific, however the main finding is the presence of leaves and seeds in the ruminal content. This reportaims to describe the clinical, epidemiological and pathological features of a spontaneous poisoning by R. communis in two sheep.Cases: During the month of March of 2015, six sheep that were kept in a native pasture with high amounts of capimannoni (Eragrostis plana) and received alfalfa hay, had access in the morning to an area infested by R. communis, which was previously cut. Two of these animals presented in the same day at the afternoon clinical signs of apathy, dyspnea and recumbency...

Comparative Immunology, Microbiology and Infectious Diseases

Brazilian Journal of Microbiology

Diseases of Aquatic Organisms

Parasitology Research

Avian malaria is a mosquito-borne disease that affects multiple avian species and is caused by pr... more Avian malaria is a mosquito-borne disease that affects multiple avian species and is caused by protozoans of the genus Plasmodium. An avian malaria infection caused by Plasmodium sp. in Magellanic penguins (Spheniscus magellanicus) with high mortality is described in a zoo in Southern Brazil. Clinically, three birds presented signs of inappetence, anorexia, pale mucosa, dyspnea, and opisthotonus, with death in a clinical course of 5-8 h. At the necropsy, all birds exhibited pale mucosa, marked splenomegaly and hepatomegaly, in addition to moderate leptomeningeal blood vessels ingurgitation in the brain. Microscopically, multiple exoerythrocytic meronts were observed in the cytoplasm of endothelial cells in the spleen, liver, heart, lungs, brain, kidneys, and pancreas. The spleen had a multifocal perivascular inflammatory infiltrate of lymphocytes, plasma cells, and macrophages, which also exhibited hemosiderosis and erythrophagocytosis. The liver had a multifocal periportal inflammatory infiltrate of lymphocytes, macrophages, and plasma cells, in addition to marked hemosiderosis in the hepatic sinusoids. Fragments of spleen, liver, brain, skeletal muscle, and lung were tested by the polymerase chain reaction technique for the detection of a fragment of the cytochrome B gene from haemosporidians, which resulted positive for Plasmodium spp. After sequencing, the samples were phylogenetically associated to Plasmodium sp. detected in Turdus albicollis (KU562808) in Brazil and matched to the lineage TURALB01 previously detected in T. albicollis. Avian malaria infections caused by Plasmodium sp. of lineage TURALB01 may occur in S. magellanicus with high mortality, and, thus, it is essential to detect and characterize the agent involved to obtain the differential diagnosis of the condition.

Virus genes, Jan 14, 2018

Bovine viral diarrhea virus 1, reclassified as Pestivirus A, causes an economically important cat... more Bovine viral diarrhea virus 1, reclassified as Pestivirus A, causes an economically important cattle disease that is distributed worldwide. Pestivirus A may cause persistent infection in that calves excrete the virus throughout their lives, spreading the infection in the herd. Many persistently infected (PI) calves die in the first 2 years of life from mucosal disease (MD) or secondary infections, probably as a consequence of virus-induced immune depression. Here, high-throughput sequencing (HTS) was applied for evaluation of the total virome in sera of (i) PI calves displaying clinically apparent MD (n = 8); (ii) PI calves with no signs of MD (n = 8); and (iii) control, Pestivirus A-free calves (n = 8). All the groups were collected at the same time and from the same herd. Serum samples from calves in each of the groups were pooled, submitted to viral RNA/DNA enrichment, and sequenced by HTS. Viral genomes of Pestivirus A, Ungulate erythroparvovirus 1, bosavirus (BosV), and hypothe...

Experimental parasitology, Jan 6, 2018

In a previous study in Brazil, six isolates of Sarcocystis spp. recovered from budgerigars fed sp... more In a previous study in Brazil, six isolates of Sarcocystis spp. recovered from budgerigars fed sporocysts excreted by opossums of the genus Didelphis were characterized by means of sequencing fragments of gene coding cytochrome B (CYTB), internal transcribed spacer 1 (ITS1), and surface antigen genes (SAG2, SAG3 and SAG4). The isolates shared identical ITS1 and CYTB sequences, but differed at SAG2, SAG3 and SAG4: three allele variants of SAG2, 3 allele variants of SAG3 and 2 allele variants of SAG4 were encountered in three multilocus genotypes (MLGs) (MLG1, MLG2, and MLG3). At ITS1 and CYTB, all the isolates from budgerigars were identical to the Sarcocystis falcatula-like isolate 59-2016-RS-BR that was detected in a barefaced ibis (Phimosus infuscatus) causing necrotizing meningoencephalitis in Brazil. At ITS1 locus, all the above isolates were clearly distinct from Sarcocystis neurona, Sarcocystis falcatula, Sarcocystis lindsayi, and Sarcocystis speeri, the four known species of ...

Veterinary pathology, Mar 1, 2017

Porcine circovirus type 2 (PCV2) is associated with multiple clinical syndromes in pigs, known as... more Porcine circovirus type 2 (PCV2) is associated with multiple clinical syndromes in pigs, known as porcine circovirus diseases. This work describes an outbreak of porcine circovirus diseases with severe lesions affecting the skeletal muscle. Ninety-two pigs had apathy, weight loss, and diarrhea over a clinical course of 7 to 10 days. Approximately 30 of the pigs had stiff gait, muscle weakness, hind limb paresis, and recumbency. Twelve of the 92 pigs were necropsied, and 4 had pale discoloration of skeletal muscles with microscopic lesions of granulomatous necrotizing myositis. Immunohistochemistry of skeletal muscle showed that PCV2 antigen was located primarily in the cytoplasm and nuclei of macrophages, lymphocytes, and multinucleated giant cells, with a lower amount in the cytoplasm of endothelial cells, necrotic fibers, and satellite cells. Affected muscle samples were polymerase chain reaction-positive for PCV2 and the amplicon exhibited 99% identity with sequences belonging to...

Journal of Equine Veterinary Science

Journal of veterinary science, Jan 10, 2017

Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, a condition termed colibacillosi... more Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, a condition termed colibacillosis, in which these bacteria should adhere and produce enterotoxins. The aim of this study was to optimize multiplex polymerase chain reaction (PCR) and immunohistochemistry (IHC) analyses of paraffin-embedded material to detect pathogenic strains of E. coli causing colibacillosis in pigs, which requires the expression of at least one fimbria and one toxin of the agent. Multiplex PCR was optimized for fimbriae (F18, F4, F6, F5, and F41) and toxins [types A and B heat-stable toxins (STaP and STb), heat-labile toxin (LT), and type 2 Shiga toxin (STx2e)], and IHC was optimized for an anti-E. coli polyclonal antibody. A total of 132 samples from pigs received by the laboratory between 2006 and 2014 with a clinical and histopathological diagnosis of colibacillosis were analyzed. E. coli was detected by IHC in 78.7%, and at least one virulence factor gene was detected in 71.2% of the cases. Path...

Semina: Ciências Agrárias, 2017

Nephroblastoma is a congenital renal neoplasm composed of metanephric blastema and stromal cells.... more Nephroblastoma is a congenital renal neoplasm composed of metanephric blastema and stromal cells. A one-year-old male Angus calf presented with diarrhea, nasal discharge, cough and weight loss. Serum levels of creatinine and urea were increased, with reduction in the level of albumin. After eight days, clinical condition got worse and the calf died. On necropsy, the cranial pole of the left kidney presented a firm whitish mass (30 x 21 x 14 cm). Multifocal to coalescing firm whitish nodules were observed on the serosa of the large intestine, in the mesentery and in the adventitia of the urinary bladder. All lung lobes contained multifocal to coalescing firm whitish elevated nodules (0.5 to 1 cm of diameter). Histologically, renal mass consisted of neoplastic proliferation of embryonal epithelial and mesenchymal cells, intermixed with large amounts of blastema cells. The nodules on large intestine, mesentery, urinary bladder and lungs were composed mainly of blastema cells and mild a...

Tropical Animal Health and Production

Ciência Rural

ABSTRACT: Bee envenomation is frequent in humans and dogs, but uncommon in horses. This study aim... more ABSTRACT: Bee envenomation is frequent in humans and dogs, but uncommon in horses. This study aimed to describe a case of acute renal failure following mass envenomation in a horse. A mare was attacked by a swarm of bees and showed reluctance to move, epistaxis, and dark-brown urine. Biochemical exams revealed increase in urea and creatine serum levels. The mare did not respond to treatment and euthanasia was elected after four days of clinical course. At the necropsy, there were multifocal pinpoint to elevated skin lesions associated with edema and hemorrhage, which extended to the subcutaneous tissue and skeletal muscle, and the kidneys were diffusely dark-brown and friable. Microscopically, renal tubules were distended and filled with an orange-red, hyaline globular material, and had severe epithelial tubular cell necrosis. The diagnosis was established based on clinical and histological analysis, and pathological evaluation was essential to confirm acute renal failure due to bee...

Medical Mycology Case Reports

Ciência Rural

ABSTRACT: Meningiomas are the most frequent neoplasms involving the brain in dogs and cats, and a... more ABSTRACT: Meningiomas are the most frequent neoplasms involving the brain in dogs and cats, and are occasionally observed in the spinal cord. They cause compression of the central nervous system; however, do not infiltrate the neuropile and rarely metastasize to other organs. The present study describes a case of anaplastic spinal meningioma with extra neural metastasis in a 20 years-old cat. Clinically, the feline presented a clinical history of 120 days with paresis of the hind limbs and loss of the tail’s movements, with subsequent death. At necropsy, there was an irregular and soft whitish mass involving the meninges from the lumbar intumescence to the sacral region of the spinal cord. Similar white nodular masses were observed in the lungs, liver and kidneys. Microscopically, both were composed of a poorly limited and infiltrative neoplastic proliferation composed by spindle, round and epithelioid cells, with a high cellular pleomorphism. On IHC, there was a severe immunostaini...

Acta Scientiae Veterinariae

Background: Cutaneous lesions in horses are common, accounting for approximately 50% of all neopl... more Background: Cutaneous lesions in horses are common, accounting for approximately 50% of all neoplasms of this species, and can cause irritability, pruritus and predispose to secondary infections. Studies have provided information of frequency of cutaneous neoplasms in horses, but few included non-neoplastic lesions. The aim of this study is to identify the frequency of cutaneous lesions in horses diagnosed in Southern Brazil, through a retrospective study and identification of the anatomic location of the lesion, the breed, gender and age of the animals affected.Materials, Methods & Results: A retrospective study on skin lesions in horses using the database of the Veterinary Pathology Laboratory located in Southern Brazil was carried out from January 2000 to February 2013. Cases were searched for and selected by diagnosis, considering age, breed, gender, location and time of occurrence. The most common lesions included sarcoid, exuberant granulation tissue, squamous cell carcinoma (...

SummaryMosquito borne flaviviruses cause a series of important diseases in humans and animals. Th... more SummaryMosquito borne flaviviruses cause a series of important diseases in humans and animals. These viruses are maintained in cycles involving replication in mosquito and in vertebrate hosts. Most natural hosts are vertebrate animals living in sylvatic or peridomestic environments. Human contact with these environments may result in host shifts that lead to the establishment of urban transmission cycles. Zika virus is a Flavivirus that persists in nature in a transmission cycle involving non-human primates (NHP). Its recent emergence in Brazil has shed light upon the importance of surveying this agent in Brazilian sylvatic environments. Here we present histopathological and molecular evidence that free ranging howler monkeys (Alouatta guariba) in Southern Brazil are infected by ZIKV closely related to African lineage MR766. Nine NHP were nested RT-PCR positive for ZIKV RNA. Sequence analysis revealed 96 to 98% identity to ZIKV MR766 and 85% identity to ZIKV P6-740, the current epid...

Journal of Veterinary Science

A 3-year-old mixed-breed female cat was diagnosed with a ventricular septal defect of the heart t... more A 3-year-old mixed-breed female cat was diagnosed with a ventricular septal defect of the heart through an echocardiogram. After a 9-month treatment, progressive and diffuse hard thickening of all limbs was observed, which on radiographic examinations, revealed a marked thickening of the long bones. The necropsy findings were limited to the appendicular skeleton and thoracic vertebrae, in addition to a severe cardiac interventricular septal defect and lung edema. The histological evaluation revealed severe replacement of the cortical bone by spongy bone in all bone fragments examined. This is the first report of hypertrophic osteopathy occurring in association with a cardiac malformation in a cat.

Ciência Rural

ABSTRACT: Meningiomas are neoplasms that commonly involve the central nervous system of cats, whi... more ABSTRACT: Meningiomas are neoplasms that commonly involve the central nervous system of cats, while cholesteatomas are nodular granulomatous chronic lesions within the choroid plexus that are rarely reported in cats. This study described a case of cholesteatoma and non-communicating hydrocephalus associated to a third ventricle meningioma in a cat. Clinically, the cat had a 2-year history of behavioral changes, photophobia and motor incoordination. At the necropsy, a tan-brown mass totally occluded the third ventricle, causing a severe dilation of the lateral ventricles (non-communicating hydrocephalus). Microscopically, the mass was composed by a neoplastic proliferation of spindle cells arranged in bundles, containing in the center psammomatous bodies (meningioma), while in the adjacent areas a cholesteatoma was observed, which was characterized by multiple cholesterol cleft formation, hemosiderosis and associated granulomatous inflammation. At immunohistochemistry (IHC), neoplast...

Revista Brasileira de Parasitologia Veterinária

Diseases related to the alimentary system are the main cause of death in horses. This retrospecti... more Diseases related to the alimentary system are the main cause of death in horses. This retrospective study aimed to describe the pathological findings of fatal parasite-induced enteritis and typhlocolitis caused by cyathostominae, Eimeria leuckarti, Balantidium coli, and Strongyloides westeri in horses. The records of parasite-induced intestinal lesions in horses necropsied in Southern Brazil between 2005 and 2017 were reviewed. Ten horses had fatal parasitic enteritis and/or typhlocolitis, and the main causes were: cyathostominae typhlocolitis (6/10), E. leuckarti enteritis (1/10), S. westeri enteritis (1/10), B. coli colitis related to cyathostominae (1/10), and infection by multiple agents (1/10). Cyathostominae typhlocolitis showed marked mucosal thickening, with multifocal elevated nodules containing tangled filiform parasites. Microscopic examination revealed that the mucosa and submucosa had encysted parasitic structures surrounded by eosinophilic and granulomatous inflammatio...

Acta Scientiae Veterinariae

Background: Ricinus communis (castor bean) is an arbustive plant, popularly known in Brazil as “m... more Background: Ricinus communis (castor bean) is an arbustive plant, popularly known in Brazil as “mamona”. This plant is widely distributed both as a weed in pastures and in specific cultures. All parts of the plant are poisonous and contain the toxic substance ricin, especially the seeds, leaves and pericarp. The ingestion of leaves and pericarp may cause neurological signs. Gross findings are unspecific, however the main finding is the presence of leaves and seeds in the ruminal content. This reportaims to describe the clinical, epidemiological and pathological features of a spontaneous poisoning by R. communis in two sheep.Cases: During the month of March of 2015, six sheep that were kept in a native pasture with high amounts of capimannoni (Eragrostis plana) and received alfalfa hay, had access in the morning to an area infested by R. communis, which was previously cut. Two of these animals presented in the same day at the afternoon clinical signs of apathy, dyspnea and recumbency...

Comparative Immunology, Microbiology and Infectious Diseases

Brazilian Journal of Microbiology

Diseases of Aquatic Organisms

Parasitology Research

Avian malaria is a mosquito-borne disease that affects multiple avian species and is caused by pr... more Avian malaria is a mosquito-borne disease that affects multiple avian species and is caused by protozoans of the genus Plasmodium. An avian malaria infection caused by Plasmodium sp. in Magellanic penguins (Spheniscus magellanicus) with high mortality is described in a zoo in Southern Brazil. Clinically, three birds presented signs of inappetence, anorexia, pale mucosa, dyspnea, and opisthotonus, with death in a clinical course of 5-8 h. At the necropsy, all birds exhibited pale mucosa, marked splenomegaly and hepatomegaly, in addition to moderate leptomeningeal blood vessels ingurgitation in the brain. Microscopically, multiple exoerythrocytic meronts were observed in the cytoplasm of endothelial cells in the spleen, liver, heart, lungs, brain, kidneys, and pancreas. The spleen had a multifocal perivascular inflammatory infiltrate of lymphocytes, plasma cells, and macrophages, which also exhibited hemosiderosis and erythrophagocytosis. The liver had a multifocal periportal inflammatory infiltrate of lymphocytes, macrophages, and plasma cells, in addition to marked hemosiderosis in the hepatic sinusoids. Fragments of spleen, liver, brain, skeletal muscle, and lung were tested by the polymerase chain reaction technique for the detection of a fragment of the cytochrome B gene from haemosporidians, which resulted positive for Plasmodium spp. After sequencing, the samples were phylogenetically associated to Plasmodium sp. detected in Turdus albicollis (KU562808) in Brazil and matched to the lineage TURALB01 previously detected in T. albicollis. Avian malaria infections caused by Plasmodium sp. of lineage TURALB01 may occur in S. magellanicus with high mortality, and, thus, it is essential to detect and characterize the agent involved to obtain the differential diagnosis of the condition.

Virus genes, Jan 14, 2018

Bovine viral diarrhea virus 1, reclassified as Pestivirus A, causes an economically important cat... more Bovine viral diarrhea virus 1, reclassified as Pestivirus A, causes an economically important cattle disease that is distributed worldwide. Pestivirus A may cause persistent infection in that calves excrete the virus throughout their lives, spreading the infection in the herd. Many persistently infected (PI) calves die in the first 2 years of life from mucosal disease (MD) or secondary infections, probably as a consequence of virus-induced immune depression. Here, high-throughput sequencing (HTS) was applied for evaluation of the total virome in sera of (i) PI calves displaying clinically apparent MD (n = 8); (ii) PI calves with no signs of MD (n = 8); and (iii) control, Pestivirus A-free calves (n = 8). All the groups were collected at the same time and from the same herd. Serum samples from calves in each of the groups were pooled, submitted to viral RNA/DNA enrichment, and sequenced by HTS. Viral genomes of Pestivirus A, Ungulate erythroparvovirus 1, bosavirus (BosV), and hypothe...

Experimental parasitology, Jan 6, 2018

In a previous study in Brazil, six isolates of Sarcocystis spp. recovered from budgerigars fed sp... more In a previous study in Brazil, six isolates of Sarcocystis spp. recovered from budgerigars fed sporocysts excreted by opossums of the genus Didelphis were characterized by means of sequencing fragments of gene coding cytochrome B (CYTB), internal transcribed spacer 1 (ITS1), and surface antigen genes (SAG2, SAG3 and SAG4). The isolates shared identical ITS1 and CYTB sequences, but differed at SAG2, SAG3 and SAG4: three allele variants of SAG2, 3 allele variants of SAG3 and 2 allele variants of SAG4 were encountered in three multilocus genotypes (MLGs) (MLG1, MLG2, and MLG3). At ITS1 and CYTB, all the isolates from budgerigars were identical to the Sarcocystis falcatula-like isolate 59-2016-RS-BR that was detected in a barefaced ibis (Phimosus infuscatus) causing necrotizing meningoencephalitis in Brazil. At ITS1 locus, all the above isolates were clearly distinct from Sarcocystis neurona, Sarcocystis falcatula, Sarcocystis lindsayi, and Sarcocystis speeri, the four known species of ...

Veterinary pathology, Mar 1, 2017

Porcine circovirus type 2 (PCV2) is associated with multiple clinical syndromes in pigs, known as... more Porcine circovirus type 2 (PCV2) is associated with multiple clinical syndromes in pigs, known as porcine circovirus diseases. This work describes an outbreak of porcine circovirus diseases with severe lesions affecting the skeletal muscle. Ninety-two pigs had apathy, weight loss, and diarrhea over a clinical course of 7 to 10 days. Approximately 30 of the pigs had stiff gait, muscle weakness, hind limb paresis, and recumbency. Twelve of the 92 pigs were necropsied, and 4 had pale discoloration of skeletal muscles with microscopic lesions of granulomatous necrotizing myositis. Immunohistochemistry of skeletal muscle showed that PCV2 antigen was located primarily in the cytoplasm and nuclei of macrophages, lymphocytes, and multinucleated giant cells, with a lower amount in the cytoplasm of endothelial cells, necrotic fibers, and satellite cells. Affected muscle samples were polymerase chain reaction-positive for PCV2 and the amplicon exhibited 99% identity with sequences belonging to...

Journal of Equine Veterinary Science

Journal of veterinary science, Jan 10, 2017

Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, a condition termed colibacillosi... more Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, a condition termed colibacillosis, in which these bacteria should adhere and produce enterotoxins. The aim of this study was to optimize multiplex polymerase chain reaction (PCR) and immunohistochemistry (IHC) analyses of paraffin-embedded material to detect pathogenic strains of E. coli causing colibacillosis in pigs, which requires the expression of at least one fimbria and one toxin of the agent. Multiplex PCR was optimized for fimbriae (F18, F4, F6, F5, and F41) and toxins [types A and B heat-stable toxins (STaP and STb), heat-labile toxin (LT), and type 2 Shiga toxin (STx2e)], and IHC was optimized for an anti-E. coli polyclonal antibody. A total of 132 samples from pigs received by the laboratory between 2006 and 2014 with a clinical and histopathological diagnosis of colibacillosis were analyzed. E. coli was detected by IHC in 78.7%, and at least one virulence factor gene was detected in 71.2% of the cases. Path...

Semina: Ciências Agrárias, 2017

Nephroblastoma is a congenital renal neoplasm composed of metanephric blastema and stromal cells.... more Nephroblastoma is a congenital renal neoplasm composed of metanephric blastema and stromal cells. A one-year-old male Angus calf presented with diarrhea, nasal discharge, cough and weight loss. Serum levels of creatinine and urea were increased, with reduction in the level of albumin. After eight days, clinical condition got worse and the calf died. On necropsy, the cranial pole of the left kidney presented a firm whitish mass (30 x 21 x 14 cm). Multifocal to coalescing firm whitish nodules were observed on the serosa of the large intestine, in the mesentery and in the adventitia of the urinary bladder. All lung lobes contained multifocal to coalescing firm whitish elevated nodules (0.5 to 1 cm of diameter). Histologically, renal mass consisted of neoplastic proliferation of embryonal epithelial and mesenchymal cells, intermixed with large amounts of blastema cells. The nodules on large intestine, mesentery, urinary bladder and lungs were composed mainly of blastema cells and mild a...