Camile Mascarenhas | Universidade Federal do Rio de Janeiro (UFRJ) (original) (raw)

Papers by Camile Mascarenhas

Research paper thumbnail of Polymeric particles for the controlled release of human amylin

Colloids and Surfaces B: Biointerfaces, 2012

Research paper thumbnail of Inquérito on-line para caracterização do cuidado farmacêutico em unidades de terapia intensiva a nível nacional

Resolution No. 7 of February 24, 2010, which provides for the minimum requirements for the operat... more Resolution No. 7 of February 24, 2010, which provides for the minimum requirements for the operation of Intensive Care Units (ICU), provides that pharmaceutical assistance at the bedside must be guaranteed and integrated with the other care activities provided to the patient. The aim of this study was to characterize the pharmaceutical care provided in the ICU of Brazilian hospitals. This is an observational sectional study through an online survey with pharmacists working in the ICU for more than 3 consecutive months, which consisted of three stages. The data collection questionnaire was validated by four members of the research team, specialists and with experience in the area, representing four regions of the country, using the Delphi technique in two rounds to obtain consensus. In the pilot study, the time taken to complete the questionnaire and the electronic platform, the cognito forms, were evaluated. The collection took place over 106 days in 2019 and the link was posted on ...

Research paper thumbnail of Système polymère de confinement d'insuline, procédé et utilisation dudit système

La presente invention concerne un systeme polymere de confinement d'insuline consistant en un... more La presente invention concerne un systeme polymere de confinement d'insuline consistant en une matrice polymere, de preference sous la forme de nanoparticules et de microparticules, a liberation controlee et prolongee de l'insuline directement dans l'organisme, ainsi que son utilisation pour la preparation d'un medicament destine au traitement du diabete. Le procede de preparation de ce systeme consiste en une methode de double emulsification et d'evaporation du solvant.

Research paper thumbnail of Macromolecular confinement of therapeutic protein in polymeric particles for controlled release: insulin as a case study

Brazilian Journal of Pharmaceutical Sciences

Sustained release systems for therapeutic proteins have been widely studied targeting to improve ... more Sustained release systems for therapeutic proteins have been widely studied targeting to improve the action of these drugs. Molecular entrapping of proteins is particularly challenging due to their conformational instability. We have developed a micro-structured poly-epsilon-caprolactone (PCL) particle system loaded with human insulin using a simple double-emulsion w/o/w method followed by solvent evaporation method. This formulation is comprised by spheric-shaped microparticles with average size of 10 micrometers. In vitro release showed a biphasic behavior such as a rapid release with about 50% of drug delivered within 2 hours and a sustained phase for up to 48 h. The subcutaneous administration of microencapsulated insulin showed a biphasic effect on glycemia in streptozotocin-induced diabetic mice, compatible with short and intermediate-acting behaviors, with first transition peak at about 2 h and the second phase exerting effect for up to 48h after s.c. administration. This study reveals that a simplified double-emulsion system results in biocompatible human-insulin-loaded PCL microparticles that might be used for further development of optimized sustained release formulations of insulin to be used in the restoration of hormonal levels.

Research paper thumbnail of Polymeric particles for the controlled release of human amylin

Colloids and Surfaces B-Biointerfaces, 2012

Since its discovery the therapeutic use of the pancreatic hormone amylin has been limited due to ... more Since its discovery the therapeutic use of the pancreatic hormone amylin has been limited due to its poor water solubility and propensity for amyloid aggregation. We have entrapped the human amylin protein in polymeric nanoparticles, using a single emulsion-solvent evaporation method and investigated its effectiveness in the controlled release of the peptide. Typical preparations composed of poly--caprolactone had a mean particle size of approximately 200 nm, low polydispersity index, high protein entrapment efficiency (80%) and process yield (90%), and spherical and smooth surfaces. These nanoparticles presented a controlled release in vitro for approximately 240 h. Pharmacological evaluation in vivo by subcutaneous administration in fasting mice demonstrated the bioactivity and effectiveness of the released human amylin, resulting in reduced glycemia lasting for at least 36 h. These features indicate the potential for the use of a confined particulate system in the therapeutic controlled and sustained release of human amylin.

Research paper thumbnail of Polymeric particles for the controlled release of human amylin

Colloids and Surfaces B: Biointerfaces, 2012

Research paper thumbnail of Inquérito on-line para caracterização do cuidado farmacêutico em unidades de terapia intensiva a nível nacional

Resolution No. 7 of February 24, 2010, which provides for the minimum requirements for the operat... more Resolution No. 7 of February 24, 2010, which provides for the minimum requirements for the operation of Intensive Care Units (ICU), provides that pharmaceutical assistance at the bedside must be guaranteed and integrated with the other care activities provided to the patient. The aim of this study was to characterize the pharmaceutical care provided in the ICU of Brazilian hospitals. This is an observational sectional study through an online survey with pharmacists working in the ICU for more than 3 consecutive months, which consisted of three stages. The data collection questionnaire was validated by four members of the research team, specialists and with experience in the area, representing four regions of the country, using the Delphi technique in two rounds to obtain consensus. In the pilot study, the time taken to complete the questionnaire and the electronic platform, the cognito forms, were evaluated. The collection took place over 106 days in 2019 and the link was posted on ...

Research paper thumbnail of Système polymère de confinement d'insuline, procédé et utilisation dudit système

La presente invention concerne un systeme polymere de confinement d'insuline consistant en un... more La presente invention concerne un systeme polymere de confinement d'insuline consistant en une matrice polymere, de preference sous la forme de nanoparticules et de microparticules, a liberation controlee et prolongee de l'insuline directement dans l'organisme, ainsi que son utilisation pour la preparation d'un medicament destine au traitement du diabete. Le procede de preparation de ce systeme consiste en une methode de double emulsification et d'evaporation du solvant.

Research paper thumbnail of Macromolecular confinement of therapeutic protein in polymeric particles for controlled release: insulin as a case study

Brazilian Journal of Pharmaceutical Sciences

Sustained release systems for therapeutic proteins have been widely studied targeting to improve ... more Sustained release systems for therapeutic proteins have been widely studied targeting to improve the action of these drugs. Molecular entrapping of proteins is particularly challenging due to their conformational instability. We have developed a micro-structured poly-epsilon-caprolactone (PCL) particle system loaded with human insulin using a simple double-emulsion w/o/w method followed by solvent evaporation method. This formulation is comprised by spheric-shaped microparticles with average size of 10 micrometers. In vitro release showed a biphasic behavior such as a rapid release with about 50% of drug delivered within 2 hours and a sustained phase for up to 48 h. The subcutaneous administration of microencapsulated insulin showed a biphasic effect on glycemia in streptozotocin-induced diabetic mice, compatible with short and intermediate-acting behaviors, with first transition peak at about 2 h and the second phase exerting effect for up to 48h after s.c. administration. This study reveals that a simplified double-emulsion system results in biocompatible human-insulin-loaded PCL microparticles that might be used for further development of optimized sustained release formulations of insulin to be used in the restoration of hormonal levels.

Research paper thumbnail of Polymeric particles for the controlled release of human amylin

Colloids and Surfaces B-Biointerfaces, 2012

Since its discovery the therapeutic use of the pancreatic hormone amylin has been limited due to ... more Since its discovery the therapeutic use of the pancreatic hormone amylin has been limited due to its poor water solubility and propensity for amyloid aggregation. We have entrapped the human amylin protein in polymeric nanoparticles, using a single emulsion-solvent evaporation method and investigated its effectiveness in the controlled release of the peptide. Typical preparations composed of poly--caprolactone had a mean particle size of approximately 200 nm, low polydispersity index, high protein entrapment efficiency (80%) and process yield (90%), and spherical and smooth surfaces. These nanoparticles presented a controlled release in vitro for approximately 240 h. Pharmacological evaluation in vivo by subcutaneous administration in fasting mice demonstrated the bioactivity and effectiveness of the released human amylin, resulting in reduced glycemia lasting for at least 36 h. These features indicate the potential for the use of a confined particulate system in the therapeutic controlled and sustained release of human amylin.