Debora Ornellas | Universidade Federal do Rio de Janeiro (UFRJ) (original) (raw)

Papers by Debora Ornellas

Research paper thumbnail of Early effects of bone marrow-derived mononuclear cells on lung and kidney in experimental sepsis

Respiratory Physiology & Neurobiology

Research paper thumbnail of Índice De Herdabilidade Na Antropometria e Lateralidade De Gêmeos Do Município De Rio De Janeiro

Revista de Educação Física / Journal of Physical Education, 1970

Resumo: A competitividade no âmbito do esporte de alto nível tem despertado interesse por pesquis... more Resumo: A competitividade no âmbito do esporte de alto nível tem despertado interesse por pesquisas no campo da genética. O objetivo geral deste estudo foi verifi car o índice de herdabilidade nas variáveis antropométricas e lateralidade em gêmeos do município de Rio de Janeiro. Participaram da amostra 32 pares de gêmeos de ambos os sexos, sendo 23 pares monozigóticos (9 pares do sexo masculino e 14 pares do sexo feminino) e 9 pares dizigóticos (5 pares do sexo masculino e 4 pares do sexo feminino),compreendendo uma faixa etária de 7 a 40 anos de idade. Todos os gêmeos participantes eram residentes de área urbana pertencentes à capital do Estado do Rio de Janeiro, sendo estes, praticantes ou não praticantes de atividade física. A zigosidade foi obtida pelo Questionário de Peeters (1998) e a lateralidade por meio de uma anamnese de auto-relato. Para as medidas antropométricas de massa corporal total e estatura, adotou-se as padronizações internacionais da ISAK (2000). Para análise estatística aplicou-se uma análise multivariada, (técnica de Cluster analysis) e nível de signifi cância p < 0,05. Toda a análise estatística foi realizada no Programa Estatístico Statistical Package for Social Sciences (SPSS) for Windows versão 13.0, ano 2006. O estudo demonstrou que as variáveis determinadas essencialmente pela infl uência ambiental são: massa corporal total (h 2 = 55,5%), índice de massa corporal (h 2 = 41%) e lateralidade / preferência lateral (H= 0), diferente da estatura, determinada pela herança genética do indivíduo (h 2 = 76,1%). O conhecimento prévio das variáveis determinadas predominantemente por herança genética e daquelas determinadas pela infl uência do meio, portanto treináveis, são fundamentais, pois auxilia do processo de orientação esportiva, bem como no treinamento, possibilitando o êxito esportivo.

Research paper thumbnail of Bone Marrow Mononuclear Cell Therapy Led to a Better Morpho-Functional Response in Extrapulmonary Compared to Pulmonary Acute Lung Injury

B19. NOVEL MECHANISMS IN ACUTE LUNG INJURY, 2009

B19 NOVEL MECHANISMS IN ACUTE LUNG INJURY / Mini Symposium / Monday, May 18/8:15 AM−10:45 AM / Ro... more B19 NOVEL MECHANISMS IN ACUTE LUNG INJURY / Mini Symposium / Monday, May 18/8:15 AM−10:45 AM / Room 3 (Upper ... Bone Marrow Mononuclear Cell Therapy Led to a Better Morpho−Functional Response in Extrapulmonary Compared to Pulmonary Acute ...

Research paper thumbnail of Thyroid hormone modulates ClC-2 chloride channel gene expression in rat renal proximal tubules

Journal of Endocrinology, 2003

Thyroid hormones has its main role in controlling metabolism, but it can also modulate extracellu... more Thyroid hormones has its main role in controlling metabolism, but it can also modulate extracellular fluid Volume (ECFV) through its action on the expression and activity of Na(+) transporters. Otherwise, chloride is the main anion in the ECFV and the influence of thyroid hormones in the regulation of chloride transporters is not yet understood. In this work, we studied the effect of thyroid hormones in the expression of ClC-2, a cell Volume-, pH- and voltage-sensitive Cl(-) channel, in rat kidney. To analyze the modulation of ClC-2 gene expression by thyroid hormones, we used hypothyroid (Hypo) rats with or without thyroxine (T(4)) replacement and hyperthyroid (Hyper) rats as our experimental models. Total RNA was isolated and the expression of ClC-2 mRNA was evaluated by a ribonuclease protection assay, and/or semi-quantitative RT-PCR. Renal ClC-2 expression decreased in Hypo rats and increased in Hyper rats. In addition, semi-quantitative RT-PCR of different nephron segments show...

Research paper thumbnail of Brilliant Blue G, a P2X7 receptor antagonist, attenuates early phase of renal inflammation, interstitial fibrosis and is associated with renal cell proliferation in ureteral obstruction in rats

Background Previous study showed that purinergic P2X7 receptors (P2X7R) reach the highest express... more Background Previous study showed that purinergic P2X7 receptors (P2X7R) reach the highest expression in the first week after unilateral ureteral obstruction (UUO) in mice, and are involved in the process of inflammation, apoptosis and fibrosis of renal tissue. We, herein, document the role of purinergic P2X7 receptors activation on the third day of UUO, as assessed by means of BBG as its selective inhibitor.Methods We investigated the effects of brilliant blue G (BBG), a P2X7R antagonist, in the third day of kidney tissue response to UUO in rats. For this purpose, male Wistar rats submitted to UUO or sham operated, received BBG or vehicle (V), comprising four groups: UUO-BBG, UUO-V, sham-BBG and sham-V. The kidneys were harvested on day 3 UUO and prepared for histology, immunohistochemistry (P2X7R, PCNA, CD-68, α-sma, TGF-β1, Heat-shock protein-47, TUNEL assay), quantitative real-time PCR (IL-1β, procollagens type I, III, and IV) for mRNA quantification.Results The group UUO-V prese...

Research paper thumbnail of Lung Mechanical Stress Induced by Recruitment Maneuver in Different Degrees of Acute Lung Injury

B106. MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY, 2009

B106 MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY / Poster Discussion / Monday, May 18/1:30 PM... more B106 MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY / Poster Discussion / Monday, May 18/1:30 PM−4:00 PM / ... Room 16 A−B (Mezzanine Level) San Diego Convention Center ... Lung Mechanical Stress Induced by Recruitment Maneuver in Different ...

Research paper thumbnail of Transcriptional adaptation to Clcn5 knockout in proximal tubules of mouse kidney

Physiological Genomics, 2008

Dent disease has multiple defects attributed to proximal tubule malfunction including low-molecul... more Dent disease has multiple defects attributed to proximal tubule malfunction including low-molecular-weight proteinuria, aminoaciduria, phosphaturia, and glycosuria. To understand the changes in kidney function of the Clc5 chloride/proton exchanger gene knockout mouse model of Dent disease, we examined gene expression profiles from proximal S1 and S2 tubules of mouse kidneys. We found many changes in gene expression not known previously to be altered in this disease. Genes involved in lipid metabolism, organ development, and organismal physiological processes had the greatest number of significantly changed transcripts. In addition, genes of catalytic activity and transporter activity also had a great number of changed transcripts. Overall, 720 genes are expressed differentially in the proximal tubules of the Dent Clcn5 knockout mouse model compared with those of control wild-type mice. The fingerprint of these gene changes may help us to understand the phenotype of Dent disease.

Research paper thumbnail of 0427. Respiratory effects of noisy ventilation depend on the etiology of acute respiratory distress syndrome

Intensive Care Medicine Experimental, 2014

Research paper thumbnail of Immunomodulatory Effects Of Sevoflurane Anesthesia In An Experimental Model Of Chronic Asthma

C33. ANTI-INFLAMMATORY STRATEGIES IN AIRWAYS DISEASE: ANIMAL MODELS, 2010

Research paper thumbnail of Prolonged recruitment manoeuvre improves lung function with less ultrastructural damage in experimental mild acute lung injury

Respiratory Physiology & Neurobiology, 2009

The effects of prolonged recruitment manoeuvre (PRM) were compared with sustained inflation (SI) ... more The effects of prolonged recruitment manoeuvre (PRM) were compared with sustained inflation (SI) in paraquat-induced mild acute lung injury (ALI) in rats. Twenty-four hours after ALI induction, rats were anesthetized and mechanically ventilated with VT=6 ml/kg and positive end-expiratory pressure (PEEP)=5 cmH(2)O for 1h. SI was performed with an instantaneous pressure increase of 40 cmH(2)O that was sustained for 40s, while PRM was done by a step-wise increase in positive inspiratory pressure (PIP) of 15-20-25 cmH(2)O above a PEEP of 15 cm H(2)O (maximal PIP=40 cmH(2)O), with interposed periods of PIP=10 cmH(2)O above a PEEP=15 cmH(2)O. Lung static elastance and the amount of alveolar collapse were more reduced with PRM than SI, yielding improved oxygenation. Additionally, tumour necrosis factor-alpha, interleukin-6, interferon-gamma, and type III procollagen mRNA expressions in lung tissue and lung epithelial cell apoptosis decreased more in PRM. In conclusion, PRM improved lung function, with less damage to alveolar epithelium, resulting in reduced pulmonary injury.

Research paper thumbnail of Intratracheal instillation of bone marrow-derived cell in an experimental model of silicosis

Respiratory Physiology & Neurobiology, 2009

The time course of lung mechanics, histology, and inflammatory and fibrogenic mediators are analy... more The time course of lung mechanics, histology, and inflammatory and fibrogenic mediators are analysed after intratracheal instillation (IT) of bone marrow-derived cells (BMDC) in a model of silicosis. C57BL/6 mice were randomly divided into SIL (silica, 20mg IT) and control (CTRL) groups (saline IT). At day 15, mice received saline or BMDC (2 x 10(6)cells) IT. The biodistribution of technetium-99m BMDC was higher in lungs compared with other organs. At days 30 and 60, lung mechanics, the area of granulomatous nodules, and mRNA expression of IL-1beta and TGF-beta were higher in SIL than CTRL animals. BMDC minimized changes in lung mechanics, the area of granulomatous nodules, and total cell infiltration at day 30, but these effects were no longer observed at day 60. Conversely, BMDC avoided the expression of IL-1beta at days 30 and 60 and TGF-beta only at day 30. In conclusion, BMDC therapy improved lung mechanics and histology, but this beneficial effect was not maintained in the course of injury.

Research paper thumbnail of Anti-inflammatory effects of sevoflurane anesthesia in an experimental model of chronic allergic asthma

Recent investigations reported that sevoflurane anaesthesia has anti-inflammatory effects in seps... more Recent investigations reported that sevoflurane anaesthesia has anti-inflammatory effects in sepsis. However, there are no studies, so far, examining the anti-inflammatory effects of sevoflurane in a chronically inflamed and remodeled airway, such as that found in asthma. For this purpose, lung histology and gene expression of pro- and anti-inflammatory cytokines were analysed. Thirty-six BALB/c mice (20-25 g) were randomly divided into four groups (n=9). In OVA groups, mice were sensitized with ovalbumin and exposed to repeated ovalbumin challenges. In SAL groups, mice received saline using the same protocol. Twenty-four hours after the last challenge, the animals were anesthetized with pentobarbital sodium (PENTO, 20 mg/kg i.p.) or sevoflurane (SEVO,1MAC). Lung static elastance, airway resistance, and viscoelastic/inhomogeneous pressure were analysed by end-inflation occlusion method. Left lungs were fixed and stained for histological analysis. In addition, we measured tumour necr...

Research paper thumbnail of Sepsis-Induced

Hypervolemia induces and potentiates lung damage after recruitment maneuver in a model of

Research paper thumbnail of Erratum: 'Atrial natriuretic peptide modulates cystic fibrosis transmembrane conductance regulator chloride channel expression in rat proximal colon and human intestinal epithelial cells' (Journal of Endocrinology (2006) vol. 189 (155-156))

Research paper thumbnail of Damage After Recruitment Maneuver in a Model of Sepsis-Induced Acute Lung Injury

Introduction: Recruitment maneuvers (RMs) seem to be more effective in extrapulmonary acute lung ... more Introduction: Recruitment maneuvers (RMs) seem to be more effective in extrapulmonary acute lung injury (ALI), caused mainly by sepsis, than in pulmonary ALI. Nevertheless, the maintenance of adequate volemic status is particularly challenging in sepsis. Since the interaction between volemic status and RMs is not well established, we investigated the effects of RMs on lung and distal organs in the presence of hypovolemia, normovolemia, and hypervolemia in a model of extrapulmonary lung injury induced by sepsis. Methods: ALI was induced by cecal ligation and puncture surgery in 66 Wistar rats. After 48 h, animals were anesthetized, mechanically ventilated and randomly assigned to 3 volemic status (n = 22/group): 1) hypovolemia induced by blood drainage at mean arterial pressure (MAP)≈70 mmHg; 2) normovolemia (MAP≈100 mmHg), and 3) hypervolemia with colloid administration to achieve a MAP≈130 mmHg. In each group, animals were further randomized to be recruited (CPAP = 40 cm H 2 O for ...

Research paper thumbnail of Lung Functional and Biologic Responses to Variable Ventilation in Experimental Pulmonary and Extrapulmonary Acute Respiratory Distress Syndrome

Critical care medicine, Jan 9, 2016

The biologic effects of variable ventilation may depend on the etiology of acute respiratory dist... more The biologic effects of variable ventilation may depend on the etiology of acute respiratory distress syndrome. We compared variable and conventional ventilation in experimental pulmonary and extrapulmonary acute respiratory distress syndrome. Prospective, randomized, controlled experimental study. University research laboratory. Twenty-four Wistar rats. Acute respiratory distress syndrome was induced by Escherichia coli lipopolysaccharide administered intratracheally (pulmonary acute respiratory distress syndrome, n = 12) or intraperitoneally (extrapulmonary acute respiratory distress syndrome, n = 12). After 24 hours, animals were randomly assigned to receive conventional (volume-controlled ventilation, n = 6) or variable ventilation (n = 6). Nonventilated animals (n = 4 per etiology) were used for comparison of diffuse alveolar damage, E-cadherin, and molecular biology variables. Variable ventilation was applied on a breath-to-breath basis as a sequence of randomly generated tida...

Research paper thumbnail of Modulation of Stress versus Time Product during Mechanical Ventilation Influences Inflammation as Well as Alveolar Epithelial and Endothelial Response in Rats

Anesthesiology, 2015

Background: Mechanical ventilation can lead to lung biotrauma when mechanical stress exceeds safe... more Background: Mechanical ventilation can lead to lung biotrauma when mechanical stress exceeds safety thresholds. The authors investigated whether the duration of mechanical stress, that is, the impact of a stress versus time product (STP), influences biotrauma. The authors hypothesized that higher STP levels are associated with increased inflammation and with alveolar epithelial and endothelial cell injury. Methods: In 46 rats, Escherichia coli lipopolysaccharide (acute lung inflammation) or saline (control) was administered intratracheally. Both groups were protectively ventilated with inspiratory-to-expiratory ratios 1:2, 1:1, or 2:1 (n = 12 each), corresponding to low, middle, and high STP levels (STPlow, STPmid, and STPhigh, respectively). The remaining 10 animals were not mechanically ventilated. Results: In animals with mild acute lung inflammation, but not in controls: (1) messenger RNA expression of interleukin-6 was higher in STPhigh (28.1 ± 13.6; mean ± SD) and STPlow (28.9...

Research paper thumbnail of Activation of Lung Inflammation Is More Associated with Tidal Recruitment/Derecruitment Than Overdistension in an Experimental Acute Lung Injury

B106. MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY, 2009

Research paper thumbnail of Effects of Bone Marrow-Derived Stem Cell Therapy on Airway and Lung Parenchyma Remodeling in a Murine Model of Chronic Allergic Asthma

American Journal of …, 2009

D29 LUNG REMODELING: NEW CHALLENGES AND PROMISES / Poster Discussion / Wednesday, May 20/8:15 AM−... more D29 LUNG REMODELING: NEW CHALLENGES AND PROMISES / Poster Discussion / Wednesday, May 20/8:15 AM−10:45 AM / Room 16 A−B (Mezzanine Level) San Diego Convention Center ... Effects of Bone Marrow−Derived Stem Cell Therapy on Airway and Lung ...

Research paper thumbnail of Protective effects of bone marrow mononuclear cell therapy on lung and heart in an elastase-induced emphysema model

We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and c... more We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently the heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E group) once a week during 4 weeks. C and E groups were randomized into subgroups receiving saline (SAL) or male BMDMCs (2 × 10 6 , CELL) intravenously 3 h after the first saline or elastase instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle wall thickness and area, higher endothelial growth factor and insulin-like growth factor mRNA expressions in lung tissue, and reduced platelet-derived growth factor, transforming growth factor-␤, and caspase-3 expressions. In conclusion, BMDMC therapy was effective at modulating the inflammatory and remodeling processes in the present model of elastase-induced emphysema.

Research paper thumbnail of Early effects of bone marrow-derived mononuclear cells on lung and kidney in experimental sepsis

Respiratory Physiology & Neurobiology

Research paper thumbnail of Índice De Herdabilidade Na Antropometria e Lateralidade De Gêmeos Do Município De Rio De Janeiro

Revista de Educação Física / Journal of Physical Education, 1970

Resumo: A competitividade no âmbito do esporte de alto nível tem despertado interesse por pesquis... more Resumo: A competitividade no âmbito do esporte de alto nível tem despertado interesse por pesquisas no campo da genética. O objetivo geral deste estudo foi verifi car o índice de herdabilidade nas variáveis antropométricas e lateralidade em gêmeos do município de Rio de Janeiro. Participaram da amostra 32 pares de gêmeos de ambos os sexos, sendo 23 pares monozigóticos (9 pares do sexo masculino e 14 pares do sexo feminino) e 9 pares dizigóticos (5 pares do sexo masculino e 4 pares do sexo feminino),compreendendo uma faixa etária de 7 a 40 anos de idade. Todos os gêmeos participantes eram residentes de área urbana pertencentes à capital do Estado do Rio de Janeiro, sendo estes, praticantes ou não praticantes de atividade física. A zigosidade foi obtida pelo Questionário de Peeters (1998) e a lateralidade por meio de uma anamnese de auto-relato. Para as medidas antropométricas de massa corporal total e estatura, adotou-se as padronizações internacionais da ISAK (2000). Para análise estatística aplicou-se uma análise multivariada, (técnica de Cluster analysis) e nível de signifi cância p < 0,05. Toda a análise estatística foi realizada no Programa Estatístico Statistical Package for Social Sciences (SPSS) for Windows versão 13.0, ano 2006. O estudo demonstrou que as variáveis determinadas essencialmente pela infl uência ambiental são: massa corporal total (h 2 = 55,5%), índice de massa corporal (h 2 = 41%) e lateralidade / preferência lateral (H= 0), diferente da estatura, determinada pela herança genética do indivíduo (h 2 = 76,1%). O conhecimento prévio das variáveis determinadas predominantemente por herança genética e daquelas determinadas pela infl uência do meio, portanto treináveis, são fundamentais, pois auxilia do processo de orientação esportiva, bem como no treinamento, possibilitando o êxito esportivo.

Research paper thumbnail of Bone Marrow Mononuclear Cell Therapy Led to a Better Morpho-Functional Response in Extrapulmonary Compared to Pulmonary Acute Lung Injury

B19. NOVEL MECHANISMS IN ACUTE LUNG INJURY, 2009

B19 NOVEL MECHANISMS IN ACUTE LUNG INJURY / Mini Symposium / Monday, May 18/8:15 AM−10:45 AM / Ro... more B19 NOVEL MECHANISMS IN ACUTE LUNG INJURY / Mini Symposium / Monday, May 18/8:15 AM−10:45 AM / Room 3 (Upper ... Bone Marrow Mononuclear Cell Therapy Led to a Better Morpho−Functional Response in Extrapulmonary Compared to Pulmonary Acute ...

Research paper thumbnail of Thyroid hormone modulates ClC-2 chloride channel gene expression in rat renal proximal tubules

Journal of Endocrinology, 2003

Thyroid hormones has its main role in controlling metabolism, but it can also modulate extracellu... more Thyroid hormones has its main role in controlling metabolism, but it can also modulate extracellular fluid Volume (ECFV) through its action on the expression and activity of Na(+) transporters. Otherwise, chloride is the main anion in the ECFV and the influence of thyroid hormones in the regulation of chloride transporters is not yet understood. In this work, we studied the effect of thyroid hormones in the expression of ClC-2, a cell Volume-, pH- and voltage-sensitive Cl(-) channel, in rat kidney. To analyze the modulation of ClC-2 gene expression by thyroid hormones, we used hypothyroid (Hypo) rats with or without thyroxine (T(4)) replacement and hyperthyroid (Hyper) rats as our experimental models. Total RNA was isolated and the expression of ClC-2 mRNA was evaluated by a ribonuclease protection assay, and/or semi-quantitative RT-PCR. Renal ClC-2 expression decreased in Hypo rats and increased in Hyper rats. In addition, semi-quantitative RT-PCR of different nephron segments show...

Research paper thumbnail of Brilliant Blue G, a P2X7 receptor antagonist, attenuates early phase of renal inflammation, interstitial fibrosis and is associated with renal cell proliferation in ureteral obstruction in rats

Background Previous study showed that purinergic P2X7 receptors (P2X7R) reach the highest express... more Background Previous study showed that purinergic P2X7 receptors (P2X7R) reach the highest expression in the first week after unilateral ureteral obstruction (UUO) in mice, and are involved in the process of inflammation, apoptosis and fibrosis of renal tissue. We, herein, document the role of purinergic P2X7 receptors activation on the third day of UUO, as assessed by means of BBG as its selective inhibitor.Methods We investigated the effects of brilliant blue G (BBG), a P2X7R antagonist, in the third day of kidney tissue response to UUO in rats. For this purpose, male Wistar rats submitted to UUO or sham operated, received BBG or vehicle (V), comprising four groups: UUO-BBG, UUO-V, sham-BBG and sham-V. The kidneys were harvested on day 3 UUO and prepared for histology, immunohistochemistry (P2X7R, PCNA, CD-68, α-sma, TGF-β1, Heat-shock protein-47, TUNEL assay), quantitative real-time PCR (IL-1β, procollagens type I, III, and IV) for mRNA quantification.Results The group UUO-V prese...

Research paper thumbnail of Lung Mechanical Stress Induced by Recruitment Maneuver in Different Degrees of Acute Lung Injury

B106. MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY, 2009

B106 MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY / Poster Discussion / Monday, May 18/1:30 PM... more B106 MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY / Poster Discussion / Monday, May 18/1:30 PM−4:00 PM / ... Room 16 A−B (Mezzanine Level) San Diego Convention Center ... Lung Mechanical Stress Induced by Recruitment Maneuver in Different ...

Research paper thumbnail of Transcriptional adaptation to Clcn5 knockout in proximal tubules of mouse kidney

Physiological Genomics, 2008

Dent disease has multiple defects attributed to proximal tubule malfunction including low-molecul... more Dent disease has multiple defects attributed to proximal tubule malfunction including low-molecular-weight proteinuria, aminoaciduria, phosphaturia, and glycosuria. To understand the changes in kidney function of the Clc5 chloride/proton exchanger gene knockout mouse model of Dent disease, we examined gene expression profiles from proximal S1 and S2 tubules of mouse kidneys. We found many changes in gene expression not known previously to be altered in this disease. Genes involved in lipid metabolism, organ development, and organismal physiological processes had the greatest number of significantly changed transcripts. In addition, genes of catalytic activity and transporter activity also had a great number of changed transcripts. Overall, 720 genes are expressed differentially in the proximal tubules of the Dent Clcn5 knockout mouse model compared with those of control wild-type mice. The fingerprint of these gene changes may help us to understand the phenotype of Dent disease.

Research paper thumbnail of 0427. Respiratory effects of noisy ventilation depend on the etiology of acute respiratory distress syndrome

Intensive Care Medicine Experimental, 2014

Research paper thumbnail of Immunomodulatory Effects Of Sevoflurane Anesthesia In An Experimental Model Of Chronic Asthma

C33. ANTI-INFLAMMATORY STRATEGIES IN AIRWAYS DISEASE: ANIMAL MODELS, 2010

Research paper thumbnail of Prolonged recruitment manoeuvre improves lung function with less ultrastructural damage in experimental mild acute lung injury

Respiratory Physiology & Neurobiology, 2009

The effects of prolonged recruitment manoeuvre (PRM) were compared with sustained inflation (SI) ... more The effects of prolonged recruitment manoeuvre (PRM) were compared with sustained inflation (SI) in paraquat-induced mild acute lung injury (ALI) in rats. Twenty-four hours after ALI induction, rats were anesthetized and mechanically ventilated with VT=6 ml/kg and positive end-expiratory pressure (PEEP)=5 cmH(2)O for 1h. SI was performed with an instantaneous pressure increase of 40 cmH(2)O that was sustained for 40s, while PRM was done by a step-wise increase in positive inspiratory pressure (PIP) of 15-20-25 cmH(2)O above a PEEP of 15 cm H(2)O (maximal PIP=40 cmH(2)O), with interposed periods of PIP=10 cmH(2)O above a PEEP=15 cmH(2)O. Lung static elastance and the amount of alveolar collapse were more reduced with PRM than SI, yielding improved oxygenation. Additionally, tumour necrosis factor-alpha, interleukin-6, interferon-gamma, and type III procollagen mRNA expressions in lung tissue and lung epithelial cell apoptosis decreased more in PRM. In conclusion, PRM improved lung function, with less damage to alveolar epithelium, resulting in reduced pulmonary injury.

Research paper thumbnail of Intratracheal instillation of bone marrow-derived cell in an experimental model of silicosis

Respiratory Physiology & Neurobiology, 2009

The time course of lung mechanics, histology, and inflammatory and fibrogenic mediators are analy... more The time course of lung mechanics, histology, and inflammatory and fibrogenic mediators are analysed after intratracheal instillation (IT) of bone marrow-derived cells (BMDC) in a model of silicosis. C57BL/6 mice were randomly divided into SIL (silica, 20mg IT) and control (CTRL) groups (saline IT). At day 15, mice received saline or BMDC (2 x 10(6)cells) IT. The biodistribution of technetium-99m BMDC was higher in lungs compared with other organs. At days 30 and 60, lung mechanics, the area of granulomatous nodules, and mRNA expression of IL-1beta and TGF-beta were higher in SIL than CTRL animals. BMDC minimized changes in lung mechanics, the area of granulomatous nodules, and total cell infiltration at day 30, but these effects were no longer observed at day 60. Conversely, BMDC avoided the expression of IL-1beta at days 30 and 60 and TGF-beta only at day 30. In conclusion, BMDC therapy improved lung mechanics and histology, but this beneficial effect was not maintained in the course of injury.

Research paper thumbnail of Anti-inflammatory effects of sevoflurane anesthesia in an experimental model of chronic allergic asthma

Recent investigations reported that sevoflurane anaesthesia has anti-inflammatory effects in seps... more Recent investigations reported that sevoflurane anaesthesia has anti-inflammatory effects in sepsis. However, there are no studies, so far, examining the anti-inflammatory effects of sevoflurane in a chronically inflamed and remodeled airway, such as that found in asthma. For this purpose, lung histology and gene expression of pro- and anti-inflammatory cytokines were analysed. Thirty-six BALB/c mice (20-25 g) were randomly divided into four groups (n=9). In OVA groups, mice were sensitized with ovalbumin and exposed to repeated ovalbumin challenges. In SAL groups, mice received saline using the same protocol. Twenty-four hours after the last challenge, the animals were anesthetized with pentobarbital sodium (PENTO, 20 mg/kg i.p.) or sevoflurane (SEVO,1MAC). Lung static elastance, airway resistance, and viscoelastic/inhomogeneous pressure were analysed by end-inflation occlusion method. Left lungs were fixed and stained for histological analysis. In addition, we measured tumour necr...

Research paper thumbnail of Sepsis-Induced

Hypervolemia induces and potentiates lung damage after recruitment maneuver in a model of

Research paper thumbnail of Erratum: 'Atrial natriuretic peptide modulates cystic fibrosis transmembrane conductance regulator chloride channel expression in rat proximal colon and human intestinal epithelial cells' (Journal of Endocrinology (2006) vol. 189 (155-156))

Research paper thumbnail of Damage After Recruitment Maneuver in a Model of Sepsis-Induced Acute Lung Injury

Introduction: Recruitment maneuvers (RMs) seem to be more effective in extrapulmonary acute lung ... more Introduction: Recruitment maneuvers (RMs) seem to be more effective in extrapulmonary acute lung injury (ALI), caused mainly by sepsis, than in pulmonary ALI. Nevertheless, the maintenance of adequate volemic status is particularly challenging in sepsis. Since the interaction between volemic status and RMs is not well established, we investigated the effects of RMs on lung and distal organs in the presence of hypovolemia, normovolemia, and hypervolemia in a model of extrapulmonary lung injury induced by sepsis. Methods: ALI was induced by cecal ligation and puncture surgery in 66 Wistar rats. After 48 h, animals were anesthetized, mechanically ventilated and randomly assigned to 3 volemic status (n = 22/group): 1) hypovolemia induced by blood drainage at mean arterial pressure (MAP)≈70 mmHg; 2) normovolemia (MAP≈100 mmHg), and 3) hypervolemia with colloid administration to achieve a MAP≈130 mmHg. In each group, animals were further randomized to be recruited (CPAP = 40 cm H 2 O for ...

Research paper thumbnail of Lung Functional and Biologic Responses to Variable Ventilation in Experimental Pulmonary and Extrapulmonary Acute Respiratory Distress Syndrome

Critical care medicine, Jan 9, 2016

The biologic effects of variable ventilation may depend on the etiology of acute respiratory dist... more The biologic effects of variable ventilation may depend on the etiology of acute respiratory distress syndrome. We compared variable and conventional ventilation in experimental pulmonary and extrapulmonary acute respiratory distress syndrome. Prospective, randomized, controlled experimental study. University research laboratory. Twenty-four Wistar rats. Acute respiratory distress syndrome was induced by Escherichia coli lipopolysaccharide administered intratracheally (pulmonary acute respiratory distress syndrome, n = 12) or intraperitoneally (extrapulmonary acute respiratory distress syndrome, n = 12). After 24 hours, animals were randomly assigned to receive conventional (volume-controlled ventilation, n = 6) or variable ventilation (n = 6). Nonventilated animals (n = 4 per etiology) were used for comparison of diffuse alveolar damage, E-cadherin, and molecular biology variables. Variable ventilation was applied on a breath-to-breath basis as a sequence of randomly generated tida...

Research paper thumbnail of Modulation of Stress versus Time Product during Mechanical Ventilation Influences Inflammation as Well as Alveolar Epithelial and Endothelial Response in Rats

Anesthesiology, 2015

Background: Mechanical ventilation can lead to lung biotrauma when mechanical stress exceeds safe... more Background: Mechanical ventilation can lead to lung biotrauma when mechanical stress exceeds safety thresholds. The authors investigated whether the duration of mechanical stress, that is, the impact of a stress versus time product (STP), influences biotrauma. The authors hypothesized that higher STP levels are associated with increased inflammation and with alveolar epithelial and endothelial cell injury. Methods: In 46 rats, Escherichia coli lipopolysaccharide (acute lung inflammation) or saline (control) was administered intratracheally. Both groups were protectively ventilated with inspiratory-to-expiratory ratios 1:2, 1:1, or 2:1 (n = 12 each), corresponding to low, middle, and high STP levels (STPlow, STPmid, and STPhigh, respectively). The remaining 10 animals were not mechanically ventilated. Results: In animals with mild acute lung inflammation, but not in controls: (1) messenger RNA expression of interleukin-6 was higher in STPhigh (28.1 ± 13.6; mean ± SD) and STPlow (28.9...

Research paper thumbnail of Activation of Lung Inflammation Is More Associated with Tidal Recruitment/Derecruitment Than Overdistension in an Experimental Acute Lung Injury

B106. MECHANISMS OF VENTILATOR ASSOCIATED LUNG INJURY, 2009

Research paper thumbnail of Effects of Bone Marrow-Derived Stem Cell Therapy on Airway and Lung Parenchyma Remodeling in a Murine Model of Chronic Allergic Asthma

American Journal of …, 2009

D29 LUNG REMODELING: NEW CHALLENGES AND PROMISES / Poster Discussion / Wednesday, May 20/8:15 AM−... more D29 LUNG REMODELING: NEW CHALLENGES AND PROMISES / Poster Discussion / Wednesday, May 20/8:15 AM−10:45 AM / Room 16 A−B (Mezzanine Level) San Diego Convention Center ... Effects of Bone Marrow−Derived Stem Cell Therapy on Airway and Lung ...

Research paper thumbnail of Protective effects of bone marrow mononuclear cell therapy on lung and heart in an elastase-induced emphysema model

We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and c... more We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently the heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E group) once a week during 4 weeks. C and E groups were randomized into subgroups receiving saline (SAL) or male BMDMCs (2 × 10 6 , CELL) intravenously 3 h after the first saline or elastase instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle wall thickness and area, higher endothelial growth factor and insulin-like growth factor mRNA expressions in lung tissue, and reduced platelet-derived growth factor, transforming growth factor-␤, and caspase-3 expressions. In conclusion, BMDMC therapy was effective at modulating the inflammatory and remodeling processes in the present model of elastase-induced emphysema.