Adriana Silva | UFSCar - Federal University of São Carlos (original) (raw)

Papers by Adriana Silva

Especialist, 2013

Considerando-se a retextualização como a produção de um novo texto a partir de um ou mais textos-... more Considerando-se a retextualização como a produção de um novo texto a partir de um ou mais textos-base, relatamos, neste artigo, uma atividade na qual estudantes do Ensino Médio de uma escola pública retextualizaram uma crônica em outros diversos gêneros textuais orais e escritos. A atividade permitiu que os estudantes trabalhassem sobre estratégias linguísticas, textuais e discursivas do texto base e as projetassem em uma nova situação de interação. Os resultados sugerem que cabe especialmente à educação formal criar condições para que os estudantes conheçam e se apropriem de diferentes gêneros textuais para torná-los competentes usuários da Língua Portuguesa.

Journal of dental education, 2015

The aim of this study was to review the effectiveness of methods used for teaching dental anatomy... more The aim of this study was to review the effectiveness of methods used for teaching dental anatomy carving to dental students in operative dentistry as evaluated in published studies. This systematic review is described in accordance with the PRISMA statement. Two independent reviewers performed a systematic literature search of research published from January 1945 until May 2014. Seven databases were screened: MedLine (PubMed), Lilacs, IBECS, Web of Science, Scopus, SciELO, and The Cochrane Library. After removing duplicates, only studies using dental carving to assess the practical knowledge of anatomy were selected. The tabulated data were organized by title of article, names of authors, number of students assessed, assessment method, material used, groups tested, main results, and conclusions. The methodology quality was assessed according to the Cochrane Handbook for Systematic Reviews of Interventions. Initially, 2,258 studies were identified in all databases. Five articles met...

Objective: Burning mouth syndrome (BMS) is a chronic and painful intraoral clinical condition tha... more Objective: Burning mouth syndrome (BMS) is a chronic and painful intraoral clinical condition that is characterised by burning sensation in the clinically normal oral mucosa, without systemic or local alterations. Multiple factors have been associated with BMS, although the pathogenesis and the aetiology of BMS remain unknown. Therefore the purpose of our study was to investigate the association among polymorphisms of +3954 interleukin-1β (IL-1β), serotonin receptor gene (5-HT2A) and p53 codon 72 in BMS patients from South Brazil. Method: After approved by the Local Ethical Committee #18/2009, forty-two patients affected by BMS and 39 healthy volunteers were genotype for IL-1β, 5-HT2A and p53 codon 72 using polymerase chain reaction (PCR) amplification and digestion. Data were analyzed using Chi-square test and Fisher's exact test (p≤0.05). Result: There were no statistical differences among IL-1β, 5-HT2A and p53 codon 72 genotypes cases and control groups. However when the thre...

Revista da Faculdade de Odontologia - UPF, 2014

O uso estratégico das informações científicas e tecnológicas referentes aos dessensibilizantes de... more O uso estratégico das informações científicas e tecnológicas referentes aos dessensibilizantes dentinários pode possibilitar oportunidades de projetos científico--tecnológicos em consonância com políticas públicas de estímulo à inovação. Objetivo: Realizar um monitoramento tecnológico na área de dessensibilizantes a fim de obter um panorama que possibilite analisar o contexto atual e obter oportunidades para prospecção tecnológica. Materiais e método: Foi realizada uma busca sistemática nas bases de dados de patentes americana (USPTO), japonesa (JPO), brasileira (INPI) e europeia (EPO). Adicionalmente, o sistema Questel Orbit foi utilizado como fonte de consulta. A informação científica foi obtida das bases de dados PubMed, Scopus, Web of Science e Scielo. Foram selecionados artigos e documentos patentários referente a dessensibilizantes do período entre janeiro de 1965 e outubro de 2013. Para montagem e revisão do banco de dados, os softwares Questel Orbit e Microsoft Office Excel 2010 foram utilizados. Resultados: 261 patentes e 728 artigos satisfizeram os critérios de inclusão. Os Estados Unidos detêm 51% da prioridade de patentes, seguido pelo Japão com 20%. Além disso, os principais autores dos artigos não estão prospectando nessa tecnologia, uma vez que não houve relação entre eles e os principais inventores das patentes. Conclusões: Foi possível obter um panorama do setor de dessensibilizantes, o qual demonstra uma baixa apropriação tecnológica brasileira nessa indústria odontológica. A consolidação de políticas públicas de estímulos a projetos entre instituições científicas e tecnológicas, em áreas de fronteira de conhecimento ainda pouco exploradas, são alternativas estratégicas para uma presença efetiva da indústria brasileira nesse setor de mercado. Palavras-chave: Dessensibilizantes dentinários. Indicadores de patentes. Materiais dentários.

Psychopharmacology, 2009

Rationale Traditional remedies prepared from Ptychopetalum olacoides (PO) are used throughout the... more Rationale Traditional remedies prepared from Ptychopetalum olacoides (PO) are used throughout the Amazon to alleviate age-related conditions. These formulas are mainly used by elders, and alleged effects may be related to the anticholinesterase properties identified in a standardized ethanol extract of this species [P. olacoides standardized ethanol extract (POEE)].

Journal of Ethnopharmacology, 2004

Amazonian peoples use traditional remedies prepared with Ptychopetalum olacoides (PO) roots for t... more Amazonian peoples use traditional remedies prepared with Ptychopetalum olacoides (PO) roots for treating various age-related conditions. This study shows that a single intraperitoneally (i.p.) administration of Ptychopetalum olacoides ethanol extract (POEE, 50 and 100mg/kg) improved memory retrieval in step-down inhibitory avoidance (P <or= 0.05 and P <or= 0.01, test session latency 102 [19.38-300] and 192 [91.3-300]s, respectively versus control 24.7 [12.9-89.6]), without interfering with acquisition or consolidation in adult (2.5-month-old) mice. Comparable results were obtained with POEE given p.o. at 800 and 1000mg/kg (P <or= 0.05 and P <or= 0.01, 52.7 [19.5-297.2] and 85.7 [44.4-260.4] versus control 20.5 [8-92.6]). Moreover, memory amelioration was also observed (P…

Journal of Ethnopharmacology, 2007

Homemade remedies with Ptychopetalum olacoides (PO) roots are used by Amazonian peoples for treat... more Homemade remedies with Ptychopetalum olacoides (PO) roots are used by Amazonian peoples for treating various age-related conditions. We previously reported that Ptychopetalum olacoides ethanol extract significantly improved step-down inhibitory avoidance long-term memory in adult and reversed memory deficits in aging mice. Adding to previous data, this study shows that a single i.p. administration of Ptychopetalum olacoides ethanol extract (POEE 50 and 100 mg/kg) improved step-down inhibitory avoidance short-term memory (STM) 3 h after training in adult (2.5 month) mice; comparable results were obtained with POEE given p.o. at 800 mg/kg. Moreover, memory improvement was also observed in aging (14 months) mice presenting memory deficit as compared to adult mice. Furthermore, POEE (100 mg/kg) improved non-aversive memory systems in adult mice in an object recognition paradigm. Consistently with its traditional use this study add to previously reported data and reinforces that POEE facilitates memory processes. Although the acetylcholinesterase inhibitory properties described for this extract may be of relevance for improving memory processes, the molecular mechanism(s) underlying the memory improvement here reported needs further scrutiny.

International Endodontic Journal, 2006

Silva AF, Tarquinio SBC, Demarco FF, Piva E, Rivero ERC. The influence of haemostatic agents on h... more Silva AF, Tarquinio SBC, Demarco FF, Piva E, Rivero ERC. The influence of haemostatic agents on healing of healthy human dental pulp tissue capped with calcium hydroxide.

Clinical Oral Implants Research, 2010

Objectives: The aim of this study was to determine and compare the frequency of bacterial leakage... more Objectives: The aim of this study was to determine and compare the frequency of bacterial leakage of Streptococcus sanguinis biotype II along the implant-abutment interface between two systems of morse taper dental implants. Different methods of activation of the taper abutments were used: tapped-in (Bicon r ) and screwed-in (Ankylos s ).

Brazilian Dental Journal, 2013

This study investigated the expression of extracellular matrix glycoproteins tenascin (TN) and fi... more This study investigated the expression of extracellular matrix glycoproteins tenascin (TN) and fibronectin (FN) in pulp repair after capping with calcium hydroxide (CH), following different hemostasis protocols. Class I cavities with a pulp exposure were prepared in 42 human third molars scheduled for extraction. Different hemostatic agents (0.9% saline solution, 5.25% sodium hypochlorite and 2% chlorhexidine digluconate) were used and pulps were capped with CH cement. After 7, 30 or 90 days, teeth were extracted, formalin-fixed, and prepared for immunohistochemical technique. Hemostatic agents did not influence the expression of TN and FN. Both glycoproteins were found in the entire the pulp tissue and around collagen fibers, but were absent in the mineralized tissues. In the predentin, TN showed positive immunostaining and FN had a variable expression. Within 7 days post-treatment, a slightly more pronounced immunostaining on the pulp exposure site was observed. Within 30 days, TN...

Purpose: To test the use of commercial swine as an in vivo model for studying pulp healing in res... more Purpose: To test the use of commercial swine as an in vivo model for studying pulp healing in response to direct capping agents.

Proceedings of the National Academy of Sciences of the United States of America, Jan 29, 2015

Gene therapy has emerged as an alternative for the treatment of diseases refractory to convention... more Gene therapy has emerged as an alternative for the treatment of diseases refractory to conventional therapeutics. Synthetic nanoparticle-based gene delivery systems offer highly tunable platforms for the delivery of therapeutic genes. However, the inability to achieve sustained, high-level transgene expression in vivo presents a significant hurdle. The respiratory system, although readily accessible, remains a challenging target, as effective gene therapy mandates colloidal stability in physiological fluids and the ability to overcome biological barriers found in the lung. We formulated highly stable DNA nanoparticles based on state-of-the-art biodegradable polymers, poly(β-amino esters) (PBAEs), possessing a dense corona of polyethylene glycol. We found that these nanoparticles efficiently penetrated the nanoporous and highly adhesive human mucus gel layer that constitutes a primary barrier to reaching the underlying epithelium. We also discovered that these PBAE-based mucus-penetr...

Microscopy Research and Technique, 2015

Scanning electron microscopy (SEM) is commonly used in the analysis of scaffolds morphology, as w... more Scanning electron microscopy (SEM) is commonly used in the analysis of scaffolds morphology, as well as cell attachment, morphology and spreading on to the scaffolds. However, so far a specific methodology to prepare the alginate hydrogel (AH) scaffolds for SEM analysis has not been evaluated. This study compared different methods to fix/dehydrate cells in AH scaffolds for SEM analysis. AH scaffolds were prepared and seeded with NIH/3T3 cell line; fixed with glutaraldehyde, osmium tetroxide, or the freeze drying method and analyzed by SEM. Results demonstrated that the freeze dried method interferes less with cell morphology and density, and preserves the scaffolds structure. The fixation with glutaraldehyde did not affect cells morphology and density; however, the scaffolds morphology was affected in some level. The fixation with osmium tetroxide interfered in the natural structure of cells and scaffold. In conclusion the freeze drying and glutaraldehyde are suitable methods for cell fixation in AH scaffold for SEM, although scaffolds structure seems to be affected by glutaraldehyde. Microsc. Res. Tech., 2015. © 2015 Wiley Periodicals, Inc.

The journal of contemporary dental practice, 2014

This study evaluated the in vitro effect of 35% hydrogen peroxide (HP) on surface enamel change w... more This study evaluated the in vitro effect of 35% hydrogen peroxide (HP) on surface enamel change when activated with different light curing units (LCUs). Enamel blocks (4 × 4 × 2 mm) were obtained from bovine incisors. The initial microhardness of the enamel was determined for each specimen. After this enamel blocks were randomly divided into four groups (n = 10) and treated as follows: Control, no bleaching procedure performed; HP - LCU, application of 35% HP gel without light activation; HP + QTH, application of 35% HP gel and light activation with a Quartz Tungsten-Halogen (QTH); and HP + Light Emitting Diode, application of 35% HP gel and light-activation with a LED. New microhardness measurements were obtained, immediately, 7 and 14 days after treatment. The percentage of surface mineral change was calculated according to the baseline and post-treatment microhardness values. Additionally, six samples from each group were randomly selected and prepared for scanning electron micro...

Revista de Microbiologia, 1999

This article describes the main aspects of bovine herpesvirus type-5 (BHV-5) neurologic infection... more This article describes the main aspects of bovine herpesvirus type-5 (BHV-5) neurologic infection and disease in rabbits, a candidate animal model for studying BHV-5 neuropathogenesis. Intranasal inoculation of weanling rabbits with a Brazilian BHV-5 isolate produced neurological disease and death in 78.8% (26/33) of the animals. Neurological signs started as early as 5 days post-inoculation and lasted from 10-12 hours up to several days. Most animals evolved to a moribund state or death within 24 (69.2%) to 48 hours (88.5%). Neurological disease was characterized by excitability or depression, tremors, bruxism, walking or running in circles, backward arching of the head and body, incoordination, backward and sideways falling, paddling, profound depression and death. Moderate levels of infectivity were detected in several areas of the brain, most consistently in the ventro-lateral hemisphere (in 16 out of 20 animals), anterior cerebrum (15/20), midbrain (11/20), dorso-lateral hemisp...

Archives of oral biology, 2014

The secretion of extracellular phospholipases and proteinases of Candida has been described as a ... more The secretion of extracellular phospholipases and proteinases of Candida has been described as a relevant virulence factor in human infections. Aliphatic fatty acids have antimicrobial properties, but the mechanism by which they affect the virulence factors of microorganisms, such as Candida, is still unclear, and there are a few reports about their toxicity. The current study investigated the in vitro antifungal activity, exoenzyme production and cytotoxicity of some aliphatic fatty acids and their ester derivatives against the Candida species. The minimum inhibitory concentration and minimum fungicidal concentrations of aliphatic medium-chain fatty acids, methyl and ethyl esters were performed using the CLSI M27-A3 method and the cytotoxicity assay was performed according to ISO 10993-5. The influence of these compounds in the inhibition of the production of hydrolytic enzymes, phospholipases and proteinases by Candida was also investigated. Data analysis was performed using the o...

Veterinary parasitology, Jan 15, 2014

Three species and several genotypes of Cryptosporidium can infect the epithelial surface of the b... more Three species and several genotypes of Cryptosporidium can infect the epithelial surface of the bursa of Fabricius, the respiratory tract, the proventriculus, the intestine, and the urinary tract in birds. There is reason to believe that gastric cryptosporidiosis in birds is caused by Cryptosporidium galli and Cryptosporidium avian genotype III, resulting in a chronic illness of the proventriculus that can lead to a debilitating and fatal clinical condition in birds of the orders Passeriformes and Psittaciformes. The objectives of the present study were to develop a duplex real-time polymerase chain reaction (PCR) that targets the 18S rRNA gene to simultaneously detect C. galli and Cryptosporidium avian genotype III DNA and to compare the duplex real-time PCR results to those of nested PCR targeting a partial fragment of the 18S rRNA gene, followed by sequencing of the amplified products (nPCR/S). A total of 1027 fecal samples were collected from birds of the orders Psittaciformes a...

Especialist, 2013

Considerando-se a retextualização como a produção de um novo texto a partir de um ou mais textos-... more Considerando-se a retextualização como a produção de um novo texto a partir de um ou mais textos-base, relatamos, neste artigo, uma atividade na qual estudantes do Ensino Médio de uma escola pública retextualizaram uma crônica em outros diversos gêneros textuais orais e escritos. A atividade permitiu que os estudantes trabalhassem sobre estratégias linguísticas, textuais e discursivas do texto base e as projetassem em uma nova situação de interação. Os resultados sugerem que cabe especialmente à educação formal criar condições para que os estudantes conheçam e se apropriem de diferentes gêneros textuais para torná-los competentes usuários da Língua Portuguesa.

Journal of dental education, 2015

The aim of this study was to review the effectiveness of methods used for teaching dental anatomy... more The aim of this study was to review the effectiveness of methods used for teaching dental anatomy carving to dental students in operative dentistry as evaluated in published studies. This systematic review is described in accordance with the PRISMA statement. Two independent reviewers performed a systematic literature search of research published from January 1945 until May 2014. Seven databases were screened: MedLine (PubMed), Lilacs, IBECS, Web of Science, Scopus, SciELO, and The Cochrane Library. After removing duplicates, only studies using dental carving to assess the practical knowledge of anatomy were selected. The tabulated data were organized by title of article, names of authors, number of students assessed, assessment method, material used, groups tested, main results, and conclusions. The methodology quality was assessed according to the Cochrane Handbook for Systematic Reviews of Interventions. Initially, 2,258 studies were identified in all databases. Five articles met...

Objective: Burning mouth syndrome (BMS) is a chronic and painful intraoral clinical condition tha... more Objective: Burning mouth syndrome (BMS) is a chronic and painful intraoral clinical condition that is characterised by burning sensation in the clinically normal oral mucosa, without systemic or local alterations. Multiple factors have been associated with BMS, although the pathogenesis and the aetiology of BMS remain unknown. Therefore the purpose of our study was to investigate the association among polymorphisms of +3954 interleukin-1β (IL-1β), serotonin receptor gene (5-HT2A) and p53 codon 72 in BMS patients from South Brazil. Method: After approved by the Local Ethical Committee #18/2009, forty-two patients affected by BMS and 39 healthy volunteers were genotype for IL-1β, 5-HT2A and p53 codon 72 using polymerase chain reaction (PCR) amplification and digestion. Data were analyzed using Chi-square test and Fisher's exact test (p≤0.05). Result: There were no statistical differences among IL-1β, 5-HT2A and p53 codon 72 genotypes cases and control groups. However when the thre...

Revista da Faculdade de Odontologia - UPF, 2014

O uso estratégico das informações científicas e tecnológicas referentes aos dessensibilizantes de... more O uso estratégico das informações científicas e tecnológicas referentes aos dessensibilizantes dentinários pode possibilitar oportunidades de projetos científico--tecnológicos em consonância com políticas públicas de estímulo à inovação. Objetivo: Realizar um monitoramento tecnológico na área de dessensibilizantes a fim de obter um panorama que possibilite analisar o contexto atual e obter oportunidades para prospecção tecnológica. Materiais e método: Foi realizada uma busca sistemática nas bases de dados de patentes americana (USPTO), japonesa (JPO), brasileira (INPI) e europeia (EPO). Adicionalmente, o sistema Questel Orbit foi utilizado como fonte de consulta. A informação científica foi obtida das bases de dados PubMed, Scopus, Web of Science e Scielo. Foram selecionados artigos e documentos patentários referente a dessensibilizantes do período entre janeiro de 1965 e outubro de 2013. Para montagem e revisão do banco de dados, os softwares Questel Orbit e Microsoft Office Excel 2010 foram utilizados. Resultados: 261 patentes e 728 artigos satisfizeram os critérios de inclusão. Os Estados Unidos detêm 51% da prioridade de patentes, seguido pelo Japão com 20%. Além disso, os principais autores dos artigos não estão prospectando nessa tecnologia, uma vez que não houve relação entre eles e os principais inventores das patentes. Conclusões: Foi possível obter um panorama do setor de dessensibilizantes, o qual demonstra uma baixa apropriação tecnológica brasileira nessa indústria odontológica. A consolidação de políticas públicas de estímulos a projetos entre instituições científicas e tecnológicas, em áreas de fronteira de conhecimento ainda pouco exploradas, são alternativas estratégicas para uma presença efetiva da indústria brasileira nesse setor de mercado. Palavras-chave: Dessensibilizantes dentinários. Indicadores de patentes. Materiais dentários.

Psychopharmacology, 2009

Rationale Traditional remedies prepared from Ptychopetalum olacoides (PO) are used throughout the... more Rationale Traditional remedies prepared from Ptychopetalum olacoides (PO) are used throughout the Amazon to alleviate age-related conditions. These formulas are mainly used by elders, and alleged effects may be related to the anticholinesterase properties identified in a standardized ethanol extract of this species [P. olacoides standardized ethanol extract (POEE)].

Journal of Ethnopharmacology, 2004

Amazonian peoples use traditional remedies prepared with Ptychopetalum olacoides (PO) roots for t... more Amazonian peoples use traditional remedies prepared with Ptychopetalum olacoides (PO) roots for treating various age-related conditions. This study shows that a single intraperitoneally (i.p.) administration of Ptychopetalum olacoides ethanol extract (POEE, 50 and 100mg/kg) improved memory retrieval in step-down inhibitory avoidance (P <or= 0.05 and P <or= 0.01, test session latency 102 [19.38-300] and 192 [91.3-300]s, respectively versus control 24.7 [12.9-89.6]), without interfering with acquisition or consolidation in adult (2.5-month-old) mice. Comparable results were obtained with POEE given p.o. at 800 and 1000mg/kg (P <or= 0.05 and P <or= 0.01, 52.7 [19.5-297.2] and 85.7 [44.4-260.4] versus control 20.5 [8-92.6]). Moreover, memory amelioration was also observed (P…

Journal of Ethnopharmacology, 2007

Homemade remedies with Ptychopetalum olacoides (PO) roots are used by Amazonian peoples for treat... more Homemade remedies with Ptychopetalum olacoides (PO) roots are used by Amazonian peoples for treating various age-related conditions. We previously reported that Ptychopetalum olacoides ethanol extract significantly improved step-down inhibitory avoidance long-term memory in adult and reversed memory deficits in aging mice. Adding to previous data, this study shows that a single i.p. administration of Ptychopetalum olacoides ethanol extract (POEE 50 and 100 mg/kg) improved step-down inhibitory avoidance short-term memory (STM) 3 h after training in adult (2.5 month) mice; comparable results were obtained with POEE given p.o. at 800 mg/kg. Moreover, memory improvement was also observed in aging (14 months) mice presenting memory deficit as compared to adult mice. Furthermore, POEE (100 mg/kg) improved non-aversive memory systems in adult mice in an object recognition paradigm. Consistently with its traditional use this study add to previously reported data and reinforces that POEE facilitates memory processes. Although the acetylcholinesterase inhibitory properties described for this extract may be of relevance for improving memory processes, the molecular mechanism(s) underlying the memory improvement here reported needs further scrutiny.

International Endodontic Journal, 2006

Silva AF, Tarquinio SBC, Demarco FF, Piva E, Rivero ERC. The influence of haemostatic agents on h... more Silva AF, Tarquinio SBC, Demarco FF, Piva E, Rivero ERC. The influence of haemostatic agents on healing of healthy human dental pulp tissue capped with calcium hydroxide.

Clinical Oral Implants Research, 2010

Objectives: The aim of this study was to determine and compare the frequency of bacterial leakage... more Objectives: The aim of this study was to determine and compare the frequency of bacterial leakage of Streptococcus sanguinis biotype II along the implant-abutment interface between two systems of morse taper dental implants. Different methods of activation of the taper abutments were used: tapped-in (Bicon r ) and screwed-in (Ankylos s ).

Brazilian Dental Journal, 2013

This study investigated the expression of extracellular matrix glycoproteins tenascin (TN) and fi... more This study investigated the expression of extracellular matrix glycoproteins tenascin (TN) and fibronectin (FN) in pulp repair after capping with calcium hydroxide (CH), following different hemostasis protocols. Class I cavities with a pulp exposure were prepared in 42 human third molars scheduled for extraction. Different hemostatic agents (0.9% saline solution, 5.25% sodium hypochlorite and 2% chlorhexidine digluconate) were used and pulps were capped with CH cement. After 7, 30 or 90 days, teeth were extracted, formalin-fixed, and prepared for immunohistochemical technique. Hemostatic agents did not influence the expression of TN and FN. Both glycoproteins were found in the entire the pulp tissue and around collagen fibers, but were absent in the mineralized tissues. In the predentin, TN showed positive immunostaining and FN had a variable expression. Within 7 days post-treatment, a slightly more pronounced immunostaining on the pulp exposure site was observed. Within 30 days, TN...

Purpose: To test the use of commercial swine as an in vivo model for studying pulp healing in res... more Purpose: To test the use of commercial swine as an in vivo model for studying pulp healing in response to direct capping agents.

Proceedings of the National Academy of Sciences of the United States of America, Jan 29, 2015

Gene therapy has emerged as an alternative for the treatment of diseases refractory to convention... more Gene therapy has emerged as an alternative for the treatment of diseases refractory to conventional therapeutics. Synthetic nanoparticle-based gene delivery systems offer highly tunable platforms for the delivery of therapeutic genes. However, the inability to achieve sustained, high-level transgene expression in vivo presents a significant hurdle. The respiratory system, although readily accessible, remains a challenging target, as effective gene therapy mandates colloidal stability in physiological fluids and the ability to overcome biological barriers found in the lung. We formulated highly stable DNA nanoparticles based on state-of-the-art biodegradable polymers, poly(β-amino esters) (PBAEs), possessing a dense corona of polyethylene glycol. We found that these nanoparticles efficiently penetrated the nanoporous and highly adhesive human mucus gel layer that constitutes a primary barrier to reaching the underlying epithelium. We also discovered that these PBAE-based mucus-penetr...

Microscopy Research and Technique, 2015

Scanning electron microscopy (SEM) is commonly used in the analysis of scaffolds morphology, as w... more Scanning electron microscopy (SEM) is commonly used in the analysis of scaffolds morphology, as well as cell attachment, morphology and spreading on to the scaffolds. However, so far a specific methodology to prepare the alginate hydrogel (AH) scaffolds for SEM analysis has not been evaluated. This study compared different methods to fix/dehydrate cells in AH scaffolds for SEM analysis. AH scaffolds were prepared and seeded with NIH/3T3 cell line; fixed with glutaraldehyde, osmium tetroxide, or the freeze drying method and analyzed by SEM. Results demonstrated that the freeze dried method interferes less with cell morphology and density, and preserves the scaffolds structure. The fixation with glutaraldehyde did not affect cells morphology and density; however, the scaffolds morphology was affected in some level. The fixation with osmium tetroxide interfered in the natural structure of cells and scaffold. In conclusion the freeze drying and glutaraldehyde are suitable methods for cell fixation in AH scaffold for SEM, although scaffolds structure seems to be affected by glutaraldehyde. Microsc. Res. Tech., 2015. © 2015 Wiley Periodicals, Inc.

The journal of contemporary dental practice, 2014

This study evaluated the in vitro effect of 35% hydrogen peroxide (HP) on surface enamel change w... more This study evaluated the in vitro effect of 35% hydrogen peroxide (HP) on surface enamel change when activated with different light curing units (LCUs). Enamel blocks (4 × 4 × 2 mm) were obtained from bovine incisors. The initial microhardness of the enamel was determined for each specimen. After this enamel blocks were randomly divided into four groups (n = 10) and treated as follows: Control, no bleaching procedure performed; HP - LCU, application of 35% HP gel without light activation; HP + QTH, application of 35% HP gel and light activation with a Quartz Tungsten-Halogen (QTH); and HP + Light Emitting Diode, application of 35% HP gel and light-activation with a LED. New microhardness measurements were obtained, immediately, 7 and 14 days after treatment. The percentage of surface mineral change was calculated according to the baseline and post-treatment microhardness values. Additionally, six samples from each group were randomly selected and prepared for scanning electron micro...

Revista de Microbiologia, 1999

This article describes the main aspects of bovine herpesvirus type-5 (BHV-5) neurologic infection... more This article describes the main aspects of bovine herpesvirus type-5 (BHV-5) neurologic infection and disease in rabbits, a candidate animal model for studying BHV-5 neuropathogenesis. Intranasal inoculation of weanling rabbits with a Brazilian BHV-5 isolate produced neurological disease and death in 78.8% (26/33) of the animals. Neurological signs started as early as 5 days post-inoculation and lasted from 10-12 hours up to several days. Most animals evolved to a moribund state or death within 24 (69.2%) to 48 hours (88.5%). Neurological disease was characterized by excitability or depression, tremors, bruxism, walking or running in circles, backward arching of the head and body, incoordination, backward and sideways falling, paddling, profound depression and death. Moderate levels of infectivity were detected in several areas of the brain, most consistently in the ventro-lateral hemisphere (in 16 out of 20 animals), anterior cerebrum (15/20), midbrain (11/20), dorso-lateral hemisp...

Archives of oral biology, 2014

The secretion of extracellular phospholipases and proteinases of Candida has been described as a ... more The secretion of extracellular phospholipases and proteinases of Candida has been described as a relevant virulence factor in human infections. Aliphatic fatty acids have antimicrobial properties, but the mechanism by which they affect the virulence factors of microorganisms, such as Candida, is still unclear, and there are a few reports about their toxicity. The current study investigated the in vitro antifungal activity, exoenzyme production and cytotoxicity of some aliphatic fatty acids and their ester derivatives against the Candida species. The minimum inhibitory concentration and minimum fungicidal concentrations of aliphatic medium-chain fatty acids, methyl and ethyl esters were performed using the CLSI M27-A3 method and the cytotoxicity assay was performed according to ISO 10993-5. The influence of these compounds in the inhibition of the production of hydrolytic enzymes, phospholipases and proteinases by Candida was also investigated. Data analysis was performed using the o...

Veterinary parasitology, Jan 15, 2014

Three species and several genotypes of Cryptosporidium can infect the epithelial surface of the b... more Three species and several genotypes of Cryptosporidium can infect the epithelial surface of the bursa of Fabricius, the respiratory tract, the proventriculus, the intestine, and the urinary tract in birds. There is reason to believe that gastric cryptosporidiosis in birds is caused by Cryptosporidium galli and Cryptosporidium avian genotype III, resulting in a chronic illness of the proventriculus that can lead to a debilitating and fatal clinical condition in birds of the orders Passeriformes and Psittaciformes. The objectives of the present study were to develop a duplex real-time polymerase chain reaction (PCR) that targets the 18S rRNA gene to simultaneously detect C. galli and Cryptosporidium avian genotype III DNA and to compare the duplex real-time PCR results to those of nested PCR targeting a partial fragment of the 18S rRNA gene, followed by sequencing of the amplified products (nPCR/S). A total of 1027 fecal samples were collected from birds of the orders Psittaciformes a...