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Papers by amanda roggia ruviaro
Food Science and Technology (Campinas), 2013
Ciência Rural, 2015
O objetivo do presente estudo foi avaliar o efeito da aspersão de extratos de Lippia alba na esta... more O objetivo do presente estudo foi avaliar o efeito da aspersão de extratos de Lippia alba na estabilidade lipídica de filés de carpa húngara armazenados a -18±2°C. Filés não tratados (controle sem aspersão) ou aspergidos (1mL 10g-1 de filé) com água destilada (controle água destilada) ou com extratos de L. alba (0,10g mL-1) hidrometanólico ou aquoso foram analisados durante o armazenamento nos dias zero, 90 e 180. Independente do tempo de congelamento, o extrato hidrometanólico reduziu os valores de dienos conjugados (DC) dos filés em relação aos demais tratamentos, além de reduzir os valores de ácidos graxos livres aos 90 dias (P<0,05). O extrato aquoso resultou em maior teor de peróxidos após 180 dias de congelamento comparado aos demais tratamentos (P<0,05). Os extratos hidrometanólico e aquoso reduziram os valores de substâncias reativas ao ácido tiobarbitúrico (TBARS) após 180 dias, comparados aos filés tratados com água destilada e sem aspersão (P<0,05). Ambos os extr...
Journal of Clinical Biochemistry and Nutrition, 2012
This study explored the effects of the antioxidant astaxanthin on paraoxonase and thioredoxin red... more This study explored the effects of the antioxidant astaxanthin on paraoxonase and thioredoxin reductase activities as well as on other oxidative stress parameters and on the lipid profile in hypercholesterolemic rabbits. Rabbits were fed a standard or a hypercholesterolemic diet alone or supplemented with 50, 100 and 500 mg/100 g of astaxanthin for 60 days. Antioxidant enzymes activities, lipid profile and oxidative stress markers were evaluated in the serum. The hypercholesterolemic diet increased lipids, including unsaturated fatty acids level, whereas it decreased saturated fatty acids level. These changes were accompanied by increased levels of oxidized low-density lipoprotein and oxidized low-density lipoprotein antibodies, as well as lipid and protein oxidation. Astaxanthin (100 and 500 mg/100 g) prevented hypercholesterolemia-induced protein oxidation, whereas 500 mg/100 g of astaxanthin decreased protein oxidation per se. The activities of superoxide dismutase and thioredoxin reductase were enhanced, whereas paraoxonase activity was inhibited in hypercholesterolemic rabbits. All astaxanthin doses prevented changes in thioredoxin reductase and paraoxonase activities. This effect was not related to a direct effect of astaxanthin on these enzymes, because in vitro astaxanthin enhanced thioredoxin reductase and had no effect on paraoxonase activity. Astaxanthin could be helpful in cardiovascular diseases by restoring thioredoxin reductase and paraoxonase activities.
Lipids in Health and Disease, 2012
Background: There is a relationship among hypercholesterolemia, oxidative stress and inflammation... more Background: There is a relationship among hypercholesterolemia, oxidative stress and inflammation in the atherogenesis. Thus, the objective of the present study was to assess paraoxonase (PON1), superoxide dismutase (SOD) and thioredoxin reductase (TrxR-1) activities and their relationship with lipids, oxidative stress and inflammation in subjects with different low density lipoprotein-cholesterol (LDL) levels. Methods: Serum lipids, highly sensitive C-reactive protein (hs-CRP), lipid and protein oxidation, oxidized LDL (LDLox) and LDLox autoantibodies (LDLoxAB) levels and enzymes activities were measured in a total of 116 subjects that were divided into the following groups according to their LDL levels: low-LDL group (LDL < 100 mg/dL, n = 23), intermediate-LDL group (LDL 100-160 mg/dL, n = 50) and high-LDL group (LDL > 160 mg/dL, n = 43).
Diabetes Research and Clinical Practice, 2011
This research aimed to evaluate whether the essential oil of Aloysia triphylla (EOAT) used in viv... more This research aimed to evaluate whether the essential
oil of Aloysia triphylla (EOAT) used in vivo as a sedative in the
water for transporting fish could increase the oxidative stability of
silver catfish (Rhamdia quelen) fillets during frozen storage. The
chemical composition of EOAT and of fillets from fish exposed to
EOAT (0, 30 or 40μL L-1) were assessed. The pH and lipid oxidation
parameters (conjugated dienes, CD; thiobarbituric acid-reactivesubstances,
TBARS) were evaluated in the fillets throughout the
storage period (-18±2oC/17 months). The main compounds found
in EOAT were α- and β-citral. Treatment with EOAT did not modify
the proximate composition of the fillets, but 40μL L-1 EOAT reduced
pH levels when compared to the control fillets (P<0.05). Compared
to the control fillets, the fillets from fish treated with 30 and 40μL
L-1 EOAT had higher initial CD values (P<0.05), whereas fillets
from fish treated with 40μL L-1 EOAT had lower TBARS levels
after 6, 9 and 17 months of storage (P<0.05). Results indicated
that use of EOAT as a sedative in silver catfish transport water
delays the degradation of primary oxidation products (CD) into
secondary products (TBARS) in the frozen fillets. This delay in the
lipid oxidation rate may increase the shelf life of frozen fillets.
Basic & Clinical Pharmacology & Toxicology, 2014
Renal thioredoxin reductase-1 (TrxR-1) activity is stimulated at lead doses lower than that neces... more Renal thioredoxin reductase-1 (TrxR-1) activity is stimulated at lead doses lower than that necessary to inhibit d-aminolevulinate dehydratase activity (d-ALA-D), which is a classical early biomarker of lead effects. Thus, we hypothesized that the activity of TrxR-1 could be a more sensitive early indicator of lead effects than is d-ALA-D. To evaluate this hypothesis, we assessed the blood and renal TrxR-1 activity and its gene expression along with biomarkers of oxidative damage, antioxidant enzyme activities and biomarkers of lead exposure in rats acutely exposed to lead. A histopathological analysis was performed to verify renal damage. The increase in renal TrxR-1 activity paralleled the increase in the blood and renal lead levels at 6, 24 and 48 hr after the exposure to 25 mg/kg lead acetate (p < 0.05), whereas its expression was increased 24 and 48 hr after exposure. These effects were not accompanied by oxidative or tissue damage in the kidneys. Blood TrxR-1 activity was not affected by lead exposure (up to 25 mg/kg). Erythrocyte d-ALA-D activity was inhibited 6 hr after the exposure to 25 mg/kg lead acetate (p < 0.05) but recovered thereafter. Renal d-ALA-D activity decreased 24 and 48 hr after the exposure to 25 mg/kg lead acetate. There were no changes in any parameters at lead acetate doses <25 mg/kg. Our results indicate that blood TrxR-1 activity is not a suitable indicator of lead effects. In contrast, the increase in renal TrxR-1 expression and activity is implicated in the early events of lead exposure, most likely as a protective cellular mechanism against lead toxicity.
This study investigated the effects of hydroethanolic E. japonica seed extracts (EJSE) as inhibi... more This study investigated the effects of hydroethanolic
E. japonica seed extracts (EJSE) as inhibitors of lipid and protein
oxidation on fi sh pates subjected to refrigerated storage. Five fi sh
pate formulations were developed. These formulations included
two control pates (water-control and ascorbic acid-control)
and three pates with added EJSE (0.1, 0.2 and 0.4g of seed 100g-1
product, equivalent to 3.4, 6.8 or 13.6mg phenolic compounds
kg-1 product), which were then stored under refrigeration for 35
days. Conjugated dienes (CD) and peroxide (PV) values increased
along with the storage time; however, these values decreased and
were similar among all samples at the end of 35 days of analysis
(P<0.05). However, the thiobarbituric acid reactive substances
levels (TBARS) did not change along the storage and were not
affected by the EJSE. Additionally, there was a linear increase in
the protein carbonyl content of fi sh pates over the storage period
(P<0.05), but no effect of EJSE on protein oxidation. The results
show that, at the concentrations evaluated, hydroethanolic E.
japonica seed extract was unable to inhibit or reduce lipid and
protein oxidation in fi sh pates, but the observed phenolic content
emphasizes the need for further studies on the wastes of this fruit.
Food Science and Technology (Campinas), 2013
Ciência Rural, 2015
O objetivo do presente estudo foi avaliar o efeito da aspersão de extratos de Lippia alba na esta... more O objetivo do presente estudo foi avaliar o efeito da aspersão de extratos de Lippia alba na estabilidade lipídica de filés de carpa húngara armazenados a -18±2°C. Filés não tratados (controle sem aspersão) ou aspergidos (1mL 10g-1 de filé) com água destilada (controle água destilada) ou com extratos de L. alba (0,10g mL-1) hidrometanólico ou aquoso foram analisados durante o armazenamento nos dias zero, 90 e 180. Independente do tempo de congelamento, o extrato hidrometanólico reduziu os valores de dienos conjugados (DC) dos filés em relação aos demais tratamentos, além de reduzir os valores de ácidos graxos livres aos 90 dias (P<0,05). O extrato aquoso resultou em maior teor de peróxidos após 180 dias de congelamento comparado aos demais tratamentos (P<0,05). Os extratos hidrometanólico e aquoso reduziram os valores de substâncias reativas ao ácido tiobarbitúrico (TBARS) após 180 dias, comparados aos filés tratados com água destilada e sem aspersão (P<0,05). Ambos os extr...
Journal of Clinical Biochemistry and Nutrition, 2012
This study explored the effects of the antioxidant astaxanthin on paraoxonase and thioredoxin red... more This study explored the effects of the antioxidant astaxanthin on paraoxonase and thioredoxin reductase activities as well as on other oxidative stress parameters and on the lipid profile in hypercholesterolemic rabbits. Rabbits were fed a standard or a hypercholesterolemic diet alone or supplemented with 50, 100 and 500 mg/100 g of astaxanthin for 60 days. Antioxidant enzymes activities, lipid profile and oxidative stress markers were evaluated in the serum. The hypercholesterolemic diet increased lipids, including unsaturated fatty acids level, whereas it decreased saturated fatty acids level. These changes were accompanied by increased levels of oxidized low-density lipoprotein and oxidized low-density lipoprotein antibodies, as well as lipid and protein oxidation. Astaxanthin (100 and 500 mg/100 g) prevented hypercholesterolemia-induced protein oxidation, whereas 500 mg/100 g of astaxanthin decreased protein oxidation per se. The activities of superoxide dismutase and thioredoxin reductase were enhanced, whereas paraoxonase activity was inhibited in hypercholesterolemic rabbits. All astaxanthin doses prevented changes in thioredoxin reductase and paraoxonase activities. This effect was not related to a direct effect of astaxanthin on these enzymes, because in vitro astaxanthin enhanced thioredoxin reductase and had no effect on paraoxonase activity. Astaxanthin could be helpful in cardiovascular diseases by restoring thioredoxin reductase and paraoxonase activities.
Lipids in Health and Disease, 2012
Background: There is a relationship among hypercholesterolemia, oxidative stress and inflammation... more Background: There is a relationship among hypercholesterolemia, oxidative stress and inflammation in the atherogenesis. Thus, the objective of the present study was to assess paraoxonase (PON1), superoxide dismutase (SOD) and thioredoxin reductase (TrxR-1) activities and their relationship with lipids, oxidative stress and inflammation in subjects with different low density lipoprotein-cholesterol (LDL) levels. Methods: Serum lipids, highly sensitive C-reactive protein (hs-CRP), lipid and protein oxidation, oxidized LDL (LDLox) and LDLox autoantibodies (LDLoxAB) levels and enzymes activities were measured in a total of 116 subjects that were divided into the following groups according to their LDL levels: low-LDL group (LDL < 100 mg/dL, n = 23), intermediate-LDL group (LDL 100-160 mg/dL, n = 50) and high-LDL group (LDL > 160 mg/dL, n = 43).
Diabetes Research and Clinical Practice, 2011
This research aimed to evaluate whether the essential oil of Aloysia triphylla (EOAT) used in viv... more This research aimed to evaluate whether the essential
oil of Aloysia triphylla (EOAT) used in vivo as a sedative in the
water for transporting fish could increase the oxidative stability of
silver catfish (Rhamdia quelen) fillets during frozen storage. The
chemical composition of EOAT and of fillets from fish exposed to
EOAT (0, 30 or 40μL L-1) were assessed. The pH and lipid oxidation
parameters (conjugated dienes, CD; thiobarbituric acid-reactivesubstances,
TBARS) were evaluated in the fillets throughout the
storage period (-18±2oC/17 months). The main compounds found
in EOAT were α- and β-citral. Treatment with EOAT did not modify
the proximate composition of the fillets, but 40μL L-1 EOAT reduced
pH levels when compared to the control fillets (P<0.05). Compared
to the control fillets, the fillets from fish treated with 30 and 40μL
L-1 EOAT had higher initial CD values (P<0.05), whereas fillets
from fish treated with 40μL L-1 EOAT had lower TBARS levels
after 6, 9 and 17 months of storage (P<0.05). Results indicated
that use of EOAT as a sedative in silver catfish transport water
delays the degradation of primary oxidation products (CD) into
secondary products (TBARS) in the frozen fillets. This delay in the
lipid oxidation rate may increase the shelf life of frozen fillets.
Basic & Clinical Pharmacology & Toxicology, 2014
Renal thioredoxin reductase-1 (TrxR-1) activity is stimulated at lead doses lower than that neces... more Renal thioredoxin reductase-1 (TrxR-1) activity is stimulated at lead doses lower than that necessary to inhibit d-aminolevulinate dehydratase activity (d-ALA-D), which is a classical early biomarker of lead effects. Thus, we hypothesized that the activity of TrxR-1 could be a more sensitive early indicator of lead effects than is d-ALA-D. To evaluate this hypothesis, we assessed the blood and renal TrxR-1 activity and its gene expression along with biomarkers of oxidative damage, antioxidant enzyme activities and biomarkers of lead exposure in rats acutely exposed to lead. A histopathological analysis was performed to verify renal damage. The increase in renal TrxR-1 activity paralleled the increase in the blood and renal lead levels at 6, 24 and 48 hr after the exposure to 25 mg/kg lead acetate (p < 0.05), whereas its expression was increased 24 and 48 hr after exposure. These effects were not accompanied by oxidative or tissue damage in the kidneys. Blood TrxR-1 activity was not affected by lead exposure (up to 25 mg/kg). Erythrocyte d-ALA-D activity was inhibited 6 hr after the exposure to 25 mg/kg lead acetate (p < 0.05) but recovered thereafter. Renal d-ALA-D activity decreased 24 and 48 hr after the exposure to 25 mg/kg lead acetate. There were no changes in any parameters at lead acetate doses <25 mg/kg. Our results indicate that blood TrxR-1 activity is not a suitable indicator of lead effects. In contrast, the increase in renal TrxR-1 expression and activity is implicated in the early events of lead exposure, most likely as a protective cellular mechanism against lead toxicity.
This study investigated the effects of hydroethanolic E. japonica seed extracts (EJSE) as inhibi... more This study investigated the effects of hydroethanolic
E. japonica seed extracts (EJSE) as inhibitors of lipid and protein
oxidation on fi sh pates subjected to refrigerated storage. Five fi sh
pate formulations were developed. These formulations included
two control pates (water-control and ascorbic acid-control)
and three pates with added EJSE (0.1, 0.2 and 0.4g of seed 100g-1
product, equivalent to 3.4, 6.8 or 13.6mg phenolic compounds
kg-1 product), which were then stored under refrigeration for 35
days. Conjugated dienes (CD) and peroxide (PV) values increased
along with the storage time; however, these values decreased and
were similar among all samples at the end of 35 days of analysis
(P<0.05). However, the thiobarbituric acid reactive substances
levels (TBARS) did not change along the storage and were not
affected by the EJSE. Additionally, there was a linear increase in
the protein carbonyl content of fi sh pates over the storage period
(P<0.05), but no effect of EJSE on protein oxidation. The results
show that, at the concentrations evaluated, hydroethanolic E.
japonica seed extract was unable to inhibit or reduce lipid and
protein oxidation in fi sh pates, but the observed phenolic content
emphasizes the need for further studies on the wastes of this fruit.