Evandro Nascimento | Universidade Federal de Uberlândia (original) (raw)

Papers by Evandro Nascimento

Research paper thumbnail of Chemical Composition of the Essential Oil ofHyptis glomerataMart, ex Schrank (Lamiaceae)

Journal of Essential Oil Research, Nov 1, 2000

The chemical composition of Hyptis suaveolens was investigated and twenty three compounds were ab... more The chemical composition of Hyptis suaveolens was investigated and twenty three compounds were abundant enough to be identified by GC-MS.

Research paper thumbnail of Direct infusion electrospray ionization mass spectrometry applied to the detection of forgeries: Roasted coffees adulterated with their husks

Microchemical Journal, Nov 1, 2014

Recognition of samples of coffee adulterated with their own husks has been a challenging task. Th... more Recognition of samples of coffee adulterated with their own husks has been a challenging task. The quite similar physical aspect of roasted grinded coffee husk when compared to ground coffee hampers a prompt visual distinction among both types of products. Several methodologies that make use of distinct analytical techniques have been developed for the authentication of coffee quality. In the present work, we demonstrate that direct infusion electrospray ionization mass spectrometry (ESI-MS) can be applied to detect counterfeit samples of roasted coffee adulterated by the addition of coffee husks (at a level of 10% w/w) in a quick and reliable way. The ESI-MS fingerprints (in both the negative and positive modes) revealed the presence of diagnostic markers, such as carbohydrates (for instance, saccharose), chlorogenic acids, caffeine, and other components related to the coffee flavor, that characterize each type of sample (coffee and rusk). Furthermore, the PCA (principal component analysis) methodology, applied to the whole set of the ESI-MS data (in the negative mode), grouped the samples into three clearly distinct categories: coffees, husks and blends. The results presented herein describe therefore an innovative and rapid methodology potentially useful in the diagnosis of such hardly-detectable type of adulteration.

Research paper thumbnail of Bioactive compounds and antioxidant activity of Brazilian roasted coffees from the south of the Minas Gerais State, Brazil

22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008, 2009

Research paper thumbnail of Composition of the essential oil of leaves Qualea grandiflora and Qualea multiflora Mart. and antileishmanial activities

Pesquisas Agrárias e Ambientais: Volume III, 2021

Research paper thumbnail of Volatile Compounds Obtained by the Hydrodistillation of Sugarcane Vinasse, a Residue from Ethanol Production

Revista Virtual de Química, 2017

http://rvq.sbq.org.br Compostos voláteis obtidos por hidrodestilação da vinhaça de cana-de-açúcar... more http://rvq.sbq.org.br Compostos voláteis obtidos por hidrodestilação da vinhaça de cana-de-açúcar, um resíduo da produção de etanol Resumo: A vinhaça é o principal resíduo das indústrias de produção do etanol. Gerada em grande quantidade, ela é constituída por água, compostos orgânicos e inorgânicos e é usada extensivamente como fertilizante na agricultura, promovendo melhora na qualidade do solo e no rendimento de culturas. Entretanto, possui elevado potencial poluidor, elevada taxa de matéria orgânica, podendo levar a contaminação do solo e de águas, além do risco de lixiviação por componentes minerais. Neste estudo, os componentes voláteis da vinhaça foram obtidos por hidrodestilação em aparelho Clevenger e analisados por cromatografia a gás acoplada à espectrometria de massa (CG-EM). A mistura obtida apresentou aspecto de cera e sua análise por CG-EM indicou que é rica em ácidos graxos, álcoois e ésteres. Os componentes majoritários foram álcool feniletílico (22,28%), ácido mirístico (17,45%), ácido palmítico (15,81%), palmitato de etila (8,99%) e hexadecanol (6,06%).

Research paper thumbnail of ESTUDO DOS EXTRATIVOS DA POLPA KRAFT DE Eucalyptus. PARTE 2. BRANQUEAMENTO DOS EXTRATIVOS E DA POLPA KRAFT

Research paper thumbnail of Allelopathic potential of Pequi (Caryocar brasiliense) on Panicum maximum seeds

Research paper thumbnail of Chemical Composition of Seasonal Essential Oils from Psidium myrtoides O. Berg Leaves with Antimicrobial, Antiprotozoal, Antioxidant and Anti-inflammatory Potential Activities

Revista Virtual de Química

Psidium species are traditionally used to treat bacterial agents and as an antiproliferative subs... more Psidium species are traditionally used to treat bacterial agents and as an antiproliferative substance in human tumor cells. The current study investigated the seasonal essential oil composition of Psidium myrtoides O. Berg (Myrtaceae) leaves from the rainy and dry periods as well as some potential bioactivities. The essential oils were obtained by hydrodistillation using a Clevenger-type apparatus, with 2.0 L round-bottomed flask, with 100 g and 1.0 L of distilled water, at 97 °C, for 4 h. The obtained essential oils (EOs) were extracted with dichloromethane. The oil extraction procedure was performed in triplicate for each batch of leaves. The highest yield (1.4 ± 0.08%) was obtained in the rainy season oils. Twenty-six constituents were identified by gas-chromatography coupled to mass spectrometry (GC-MS) in the essential oils from the dry period and 22 constituents in the essential oils from the rainy season, all of them being hydrocarbons and oxygenated sesquiterpenes. The major compounds found in both periods, respectively, were β-caryophyllene (26.23%; 27.38%), α-humulene (17.40%; 16.18%), β-caryophyllene oxide (13.59%; 20.66%), humulene epoxide-II (6.09%; 10.21%), and α-copaene (8.92%; 7.11%). In general, no significant differences were observed in the chemical composition of essential oils, considering the seasonal effects and/or the status of the leaves. The antimicrobial and antiprotozoal activities were evaluated by the microdilution broth method and cytotoxic activity was carried out with Vero cells. The essential oils presented antibacterial activity against Streptococcus sanguinis (200 μg.mL-1), and antifungal activity against Candida glabrata (46.87 μg.mL-1) in Minimal Inhibitory Concentration (MIC) assays. The essential oil obtained from fresh leaves collected in the rainy season (FLRS) presented the highest Selectivity Index (0.23) against the studied promastigote forms. Therefore, the rainy volatile oils were more effective against promastigote forms of Leishmania amazonensis (31.8 ± 5.74 μg.mL-1), while the dry volatile oils were more effective against Trypanosoma cruzi (54.0 ± 5.25 μg.mL-1). Furthermore, this study investigated the antioxidant (ORAC method) capacity, using Trolox as the standard, and anti-inflammatory potential (Drosophila melanogaster of GMR-GAL4 and UAS-eiger lineage) of these oils. Thus, dry season contributed to a volatile oil (DLDS) with higher ORAC antioxidant capacity (2066.20 ± 168.20 µmol Trolox eq/g; p < 0.001) and showed no toxicity, as verified by the lineage survival test, being able to recover the eye area of the flies at 0.025-1.60 mg.mL-1 (essential oils from the dry season) and at 0.2-1.6 mg.mL-1 (essential oils from the rainy season) (p < 0.001). Therefore, the essential oils of P. myrtoides seems to be a potential therapeutical strategy against pathogens. In addition, our data suggest that the antioxidant and anti-inflammatory activities of the studied essential oils may be, at least in part, due to donation of hydrogen atoms and suppression of the Eiger pro-inflammatory cytokine, respectively.

Research paper thumbnail of Análise química de café arábica (Coffea arabica L.) e grãos pretos, verdes e ardidos (PVA) submetidos a diferentes graus de torraçao

Research paper thumbnail of TEOR DE FENÓIS TOTAIS DE PRÓPOLIS VERDES, MARROM E VER- MELHA DE REGIÕES RICAS EM ALECRIM-DO-CAMPO (Baccharis dra- cunculifolia)

Research paper thumbnail of Potencial alelopático do pequi (" Caryocar brasiliense") em sementes de" Panicum maximum

… : Lisboa, 10 a 13 de …, 2009

Información del artículo Potencial alelopático do pequi ("Caryocar brasiliense") em sem... more Información del artículo Potencial alelopático do pequi ("Caryocar brasiliense") em sementes de "Panicum maximum".

Research paper thumbnail of Atividade alelopática de extratos de" Cenchrus echinatus"(Timbete)

… : Lisboa, 10 a 13 de …, 2009

Información del artículo Atividade alelopática de extratos de "Cenchrus echinatus" (Tim... more Información del artículo Atividade alelopática de extratos de "Cenchrus echinatus" (Timbete).

Research paper thumbnail of A Porosidade do Coque da Lignina Hidrolítica de Eucalipto em Função da Pressão de Briquetagem

Research paper thumbnail of Constituintes Voláteis e Odorantes Potentes Do Café Conilon Em Diferentes Graus De Torração

Research paper thumbnail of Chemical composition and antimicrobial activity of essential oil of flowers from Banisteriopsis campestris (A. Juss.) Little

Revista Virtual de Química, 2018

The essential oil (EO) of the flowers from Banisteriopsis campestris was analysed by GC and GC-MS... more The essential oil (EO) of the flowers from Banisteriopsis campestris was analysed by GC and GC-MS. Fatty acids were the most abundant class of compounds in the EO, followed by long-chain alkanes and oxygenated sesquiterpenes and monoterpenes. The main constituents were hexadecanoic acid (39.43 %), (E)-nerolidol (10.51 %), triacontane (9.08 %), heptacosane (5.49 %) and linalool (3.23 %). The antimicrobial activity of the EO was evaluated against aerobic and anaerobic oral bacteria and some yeasts by broth microdilution method. The EO inhibited the growth of all tested oral bacteria, showing strong activity against S. sanguinis with minimum inhibitory concentration (MIC) of 25 μg mL-1. Considerable antibacterial activity was also observed for the anaerobes Porphyromonas gingivalis and Actinomyces naeslundii with MIC of 50 μg mL-1. The EO of B. campestris flower showed moderate activity for S. mutans, S. mitis, A. actinomycetemcomitans and Fusobacterium nucleatum with MICs ranging between 200 and 400 μg mL-1. The antifungal activity was evaluated against C. albicans, C. tropicalis and C. glabrata. The MIC results were above 3000 μg mL-1 , indicating inactivity against these yeasts. This study revealed that the OE of B. campestris flowers has promising antibacterial activity and this is the first report on its chemical composition and antimicrobial activity

Research paper thumbnail of Antifungal and cytotoxicity activities and new proanthocyanidins isolated from the barks of Inga laurina (Sw.) Willd

Phytochemistry Letters

Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to nort... more Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to northern Argentina. In folk medicine, Inga species are used to treat various diseases. The species Inga laurina is widely found in the Brazilian flora; however, there are few studies about its biological activity and chemical composition. The main purpose of this study was to identify and isolate the chemical constituents of Inga laurina barks and to evaluate the antifungal and cytotoxic activities. The total content of phenolics, proanthocyanidins, and flavonoids from the barks of Inga laurina were performed by spectrophotometric methods and the ethyl acetate (EAF) and n-butanol (BF) fractions showed the best results. Eleven compounds were identified in EAF by HPLC-ESI(−)-MS/MS, which showed good antifungal activity with MIC values of 23.4 and 46.8 μg mL−1, evaluated by broth microdilution method. Five new compounds of the genus Inga were isolated for the first time. Three of these compounds were isolated and reported on the literature for the first time: a proanthocyanidin B-type, gallocatechin-(4α→8)-4’-O-methylgallocatechin (XI) and two proanthocyanidins A-type, gallocatechin-(2→O→7,4→8)-4’-O-methylgallocatechin (XII) and gallocatechin-3-O-galloyl-(2→O→7,4→8)-4’-O-methylgallocatechin (XIII). The chemical study of the plant bark showed that this species is rich in phenolic compounds and it has great potential for the discovery of new bioactive compounds.

Research paper thumbnail of Antifungal and cytotoxicity activities and new proanthocyanidins isolated from the barks of Inga laurina (Sw.) Willd

Phytochemistry Letters

Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to nort... more Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to northern Argentina. In folk medicine, Inga species are used to treat various diseases. The species Inga laurina is widely found in the Brazilian flora; however, there are few studies about its biological activity and chemical composition. The main purpose of this study was to identify and isolate the chemical constituents of Inga laurina barks and to evaluate the antifungal and cytotoxic activities. The total content of phenolics, proanthocyanidins, and flavonoids from the barks of Inga laurina were performed by spectrophotometric methods and the ethyl acetate (EAF) and n-butanol (BF) fractions showed the best results. Eleven compounds were identified in EAF by HPLC-ESI(−)-MS/MS, which showed good antifungal activity with MIC values of 23.4 and 46.8 μg mL−1, evaluated by broth microdilution method. Five new compounds of the genus Inga were isolated for the first time. Three of these compounds were isolated and reported on the literature for the first time: a proanthocyanidin B-type, gallocatechin-(4α→8)-4’-O-methylgallocatechin (XI) and two proanthocyanidins A-type, gallocatechin-(2→O→7,4→8)-4’-O-methylgallocatechin (XII) and gallocatechin-3-O-galloyl-(2→O→7,4→8)-4’-O-methylgallocatechin (XIII). The chemical study of the plant bark showed that this species is rich in phenolic compounds and it has great potential for the discovery of new bioactive compounds.

Research paper thumbnail of Antimicrobial activity, cytotoxicity and selectivity index of Banisteriopsis laevifolia (A. Juss.) B. Gates leaves

Industrial Crops and Products, 2016

Abstract Banisteriopsis laevifolia (A. Juss.) B. Gates (Malpighiaceae) is largely distributed in ... more Abstract Banisteriopsis laevifolia (A. Juss.) B. Gates (Malpighiaceae) is largely distributed in the cerrado Brazilian biome. In this study, phytochemical screening, antimicrobial and antifungal activities of leaves extract and partitions were evaluated. Phytochemical screening tests indicated the presence of flavonoids, terpenoids, phenols, sugar, steroids, triterpenoids, and tannins compounds. No alkaloids or nitrogenated compounds were found. Antioxidant, antimicrobial and antifungal activities were tested. The extract and partitions from the B. laevifolia leaves demonstrated relevant scavenging free radical DPPH effect. The crude extract and partitions inhibit bacteria growth with minimum inhibitory concentrations (MIC) below 400 mg L −1 for most oral microorganisms tested. Meanwhile, the antifungal activity proved to be very promising for the ethanolic extract and partitions (31–375 μg mL −1 ) against all yeasts tested. The antimicrobial activities results are very promising since the ethanol extract and the more active n -buthanol partition showed great selectivity (0.9–1.2; 0.1–0.7, respectively) against microorganisms and relatively low toxicity to Vero cells. Analysis by UHPLC-ESI–MS n from the most bioactive fractions (ethyl acetate and n -buthanol) permitted to identify ten phenolic compounds reported in the Banisteriopsis family that exert recognized antioxidant, antimicrobial and antifungal activity. Main secondary metabolites found were phenolic acids and flavonoid glycosides, mainly derivatives from quercetin and rutin. The biological activity results and MS analysis for the B.laevifolia leaves revealed that they have efficient antimicrobial agents, and contributed to knowledge of the genus.

Research paper thumbnail of Chemical Analysis and Study of Phenolics, Antioxidant Activity, and Antibacterial Effect of the Wood and Bark ofMaclura tinctoria(L.) D. Don ex Steud

Evidence-Based Complementary and Alternative Medicine, 2012

Maclura tinctoria(L.) D. Don ex Steud. has one of the highest qualities among the coefficients fo... more Maclura tinctoria(L.) D. Don ex Steud. has one of the highest qualities among the coefficients for Brazilian woods (up to 9.6) and resistance rates equivalent to Indian teak (Tectona grandis). In this study, the macromolecular constituents and total phenols compounds as well as the antioxidant and antibacterial activities of this wood were evaluated. Total phenols and proanthocyanidin levels were higher in wood when compared with bark levels. The antioxidant activity of wood extracts (IC50= 18.7 μg/mL) was more effective than that of bark extracts (IC50= 20.9 μg/mL). Wood and bark extracts revealed a high potential for inhibition of aerobic and anaerobic bacteria. The bark extracts were the most active (MIC from 20 to 60 μg/mL). Both antioxidant activity and high potential for bacteria inhibition turn these extracts promising for drug formulations, especially as antibacterial agent.

Research paper thumbnail of Looking for Unknown Molecules in Arabica and Robusta Coffee Brews by High Performance Liquid Chromatography Coupled to High Resolution Mass Spectrometry and Differentiating both Coffee Species by this Technique

The chemical composition of coffee brews originating from four varieties of Coffeaarabica cv. (Ye... more The chemical composition of coffee brews originating from four varieties of Coffeaarabica cv. (Yellow Catuai, Mundo Novo, Topazio and Acaia) and one variety of Coffeacanephora cv. (robusta) was evaluated by high performance liquid chromatography coupled to mass Spectrometry with high resolution ((HPLC/HRMS) at different roasting degrees. The results obtained in the present study demonstrate that there are still many unknown components that can influence the coffee flavor, in addition to those reported as such. They deserve been investigated. Further, more than different constituents, the different concentrations of the same constituents are crucial for distinguishing the flavor of arabica and robusta coffee brews.

Research paper thumbnail of Chemical Composition of the Essential Oil ofHyptis glomerataMart, ex Schrank (Lamiaceae)

Journal of Essential Oil Research, Nov 1, 2000

The chemical composition of Hyptis suaveolens was investigated and twenty three compounds were ab... more The chemical composition of Hyptis suaveolens was investigated and twenty three compounds were abundant enough to be identified by GC-MS.

Research paper thumbnail of Direct infusion electrospray ionization mass spectrometry applied to the detection of forgeries: Roasted coffees adulterated with their husks

Microchemical Journal, Nov 1, 2014

Recognition of samples of coffee adulterated with their own husks has been a challenging task. Th... more Recognition of samples of coffee adulterated with their own husks has been a challenging task. The quite similar physical aspect of roasted grinded coffee husk when compared to ground coffee hampers a prompt visual distinction among both types of products. Several methodologies that make use of distinct analytical techniques have been developed for the authentication of coffee quality. In the present work, we demonstrate that direct infusion electrospray ionization mass spectrometry (ESI-MS) can be applied to detect counterfeit samples of roasted coffee adulterated by the addition of coffee husks (at a level of 10% w/w) in a quick and reliable way. The ESI-MS fingerprints (in both the negative and positive modes) revealed the presence of diagnostic markers, such as carbohydrates (for instance, saccharose), chlorogenic acids, caffeine, and other components related to the coffee flavor, that characterize each type of sample (coffee and rusk). Furthermore, the PCA (principal component analysis) methodology, applied to the whole set of the ESI-MS data (in the negative mode), grouped the samples into three clearly distinct categories: coffees, husks and blends. The results presented herein describe therefore an innovative and rapid methodology potentially useful in the diagnosis of such hardly-detectable type of adulteration.

Research paper thumbnail of Bioactive compounds and antioxidant activity of Brazilian roasted coffees from the south of the Minas Gerais State, Brazil

22nd International Conference on Coffee Science, ASIC 2008, Campinas, SP, Brazil, 14-19 September, 2008, 2009

Research paper thumbnail of Composition of the essential oil of leaves Qualea grandiflora and Qualea multiflora Mart. and antileishmanial activities

Pesquisas Agrárias e Ambientais: Volume III, 2021

Research paper thumbnail of Volatile Compounds Obtained by the Hydrodistillation of Sugarcane Vinasse, a Residue from Ethanol Production

Revista Virtual de Química, 2017

http://rvq.sbq.org.br Compostos voláteis obtidos por hidrodestilação da vinhaça de cana-de-açúcar... more http://rvq.sbq.org.br Compostos voláteis obtidos por hidrodestilação da vinhaça de cana-de-açúcar, um resíduo da produção de etanol Resumo: A vinhaça é o principal resíduo das indústrias de produção do etanol. Gerada em grande quantidade, ela é constituída por água, compostos orgânicos e inorgânicos e é usada extensivamente como fertilizante na agricultura, promovendo melhora na qualidade do solo e no rendimento de culturas. Entretanto, possui elevado potencial poluidor, elevada taxa de matéria orgânica, podendo levar a contaminação do solo e de águas, além do risco de lixiviação por componentes minerais. Neste estudo, os componentes voláteis da vinhaça foram obtidos por hidrodestilação em aparelho Clevenger e analisados por cromatografia a gás acoplada à espectrometria de massa (CG-EM). A mistura obtida apresentou aspecto de cera e sua análise por CG-EM indicou que é rica em ácidos graxos, álcoois e ésteres. Os componentes majoritários foram álcool feniletílico (22,28%), ácido mirístico (17,45%), ácido palmítico (15,81%), palmitato de etila (8,99%) e hexadecanol (6,06%).

Research paper thumbnail of ESTUDO DOS EXTRATIVOS DA POLPA KRAFT DE Eucalyptus. PARTE 2. BRANQUEAMENTO DOS EXTRATIVOS E DA POLPA KRAFT

Research paper thumbnail of Allelopathic potential of Pequi (Caryocar brasiliense) on Panicum maximum seeds

Research paper thumbnail of Chemical Composition of Seasonal Essential Oils from Psidium myrtoides O. Berg Leaves with Antimicrobial, Antiprotozoal, Antioxidant and Anti-inflammatory Potential Activities

Revista Virtual de Química

Psidium species are traditionally used to treat bacterial agents and as an antiproliferative subs... more Psidium species are traditionally used to treat bacterial agents and as an antiproliferative substance in human tumor cells. The current study investigated the seasonal essential oil composition of Psidium myrtoides O. Berg (Myrtaceae) leaves from the rainy and dry periods as well as some potential bioactivities. The essential oils were obtained by hydrodistillation using a Clevenger-type apparatus, with 2.0 L round-bottomed flask, with 100 g and 1.0 L of distilled water, at 97 °C, for 4 h. The obtained essential oils (EOs) were extracted with dichloromethane. The oil extraction procedure was performed in triplicate for each batch of leaves. The highest yield (1.4 ± 0.08%) was obtained in the rainy season oils. Twenty-six constituents were identified by gas-chromatography coupled to mass spectrometry (GC-MS) in the essential oils from the dry period and 22 constituents in the essential oils from the rainy season, all of them being hydrocarbons and oxygenated sesquiterpenes. The major compounds found in both periods, respectively, were β-caryophyllene (26.23%; 27.38%), α-humulene (17.40%; 16.18%), β-caryophyllene oxide (13.59%; 20.66%), humulene epoxide-II (6.09%; 10.21%), and α-copaene (8.92%; 7.11%). In general, no significant differences were observed in the chemical composition of essential oils, considering the seasonal effects and/or the status of the leaves. The antimicrobial and antiprotozoal activities were evaluated by the microdilution broth method and cytotoxic activity was carried out with Vero cells. The essential oils presented antibacterial activity against Streptococcus sanguinis (200 μg.mL-1), and antifungal activity against Candida glabrata (46.87 μg.mL-1) in Minimal Inhibitory Concentration (MIC) assays. The essential oil obtained from fresh leaves collected in the rainy season (FLRS) presented the highest Selectivity Index (0.23) against the studied promastigote forms. Therefore, the rainy volatile oils were more effective against promastigote forms of Leishmania amazonensis (31.8 ± 5.74 μg.mL-1), while the dry volatile oils were more effective against Trypanosoma cruzi (54.0 ± 5.25 μg.mL-1). Furthermore, this study investigated the antioxidant (ORAC method) capacity, using Trolox as the standard, and anti-inflammatory potential (Drosophila melanogaster of GMR-GAL4 and UAS-eiger lineage) of these oils. Thus, dry season contributed to a volatile oil (DLDS) with higher ORAC antioxidant capacity (2066.20 ± 168.20 µmol Trolox eq/g; p < 0.001) and showed no toxicity, as verified by the lineage survival test, being able to recover the eye area of the flies at 0.025-1.60 mg.mL-1 (essential oils from the dry season) and at 0.2-1.6 mg.mL-1 (essential oils from the rainy season) (p < 0.001). Therefore, the essential oils of P. myrtoides seems to be a potential therapeutical strategy against pathogens. In addition, our data suggest that the antioxidant and anti-inflammatory activities of the studied essential oils may be, at least in part, due to donation of hydrogen atoms and suppression of the Eiger pro-inflammatory cytokine, respectively.

Research paper thumbnail of Análise química de café arábica (Coffea arabica L.) e grãos pretos, verdes e ardidos (PVA) submetidos a diferentes graus de torraçao

Research paper thumbnail of TEOR DE FENÓIS TOTAIS DE PRÓPOLIS VERDES, MARROM E VER- MELHA DE REGIÕES RICAS EM ALECRIM-DO-CAMPO (Baccharis dra- cunculifolia)

Research paper thumbnail of Potencial alelopático do pequi (" Caryocar brasiliense") em sementes de" Panicum maximum

… : Lisboa, 10 a 13 de …, 2009

Información del artículo Potencial alelopático do pequi ("Caryocar brasiliense") em sem... more Información del artículo Potencial alelopático do pequi ("Caryocar brasiliense") em sementes de "Panicum maximum".

Research paper thumbnail of Atividade alelopática de extratos de" Cenchrus echinatus"(Timbete)

… : Lisboa, 10 a 13 de …, 2009

Información del artículo Atividade alelopática de extratos de "Cenchrus echinatus" (Tim... more Información del artículo Atividade alelopática de extratos de "Cenchrus echinatus" (Timbete).

Research paper thumbnail of A Porosidade do Coque da Lignina Hidrolítica de Eucalipto em Função da Pressão de Briquetagem

Research paper thumbnail of Constituintes Voláteis e Odorantes Potentes Do Café Conilon Em Diferentes Graus De Torração

Research paper thumbnail of Chemical composition and antimicrobial activity of essential oil of flowers from Banisteriopsis campestris (A. Juss.) Little

Revista Virtual de Química, 2018

The essential oil (EO) of the flowers from Banisteriopsis campestris was analysed by GC and GC-MS... more The essential oil (EO) of the flowers from Banisteriopsis campestris was analysed by GC and GC-MS. Fatty acids were the most abundant class of compounds in the EO, followed by long-chain alkanes and oxygenated sesquiterpenes and monoterpenes. The main constituents were hexadecanoic acid (39.43 %), (E)-nerolidol (10.51 %), triacontane (9.08 %), heptacosane (5.49 %) and linalool (3.23 %). The antimicrobial activity of the EO was evaluated against aerobic and anaerobic oral bacteria and some yeasts by broth microdilution method. The EO inhibited the growth of all tested oral bacteria, showing strong activity against S. sanguinis with minimum inhibitory concentration (MIC) of 25 μg mL-1. Considerable antibacterial activity was also observed for the anaerobes Porphyromonas gingivalis and Actinomyces naeslundii with MIC of 50 μg mL-1. The EO of B. campestris flower showed moderate activity for S. mutans, S. mitis, A. actinomycetemcomitans and Fusobacterium nucleatum with MICs ranging between 200 and 400 μg mL-1. The antifungal activity was evaluated against C. albicans, C. tropicalis and C. glabrata. The MIC results were above 3000 μg mL-1 , indicating inactivity against these yeasts. This study revealed that the OE of B. campestris flowers has promising antibacterial activity and this is the first report on its chemical composition and antimicrobial activity

Research paper thumbnail of Antifungal and cytotoxicity activities and new proanthocyanidins isolated from the barks of Inga laurina (Sw.) Willd

Phytochemistry Letters

Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to nort... more Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to northern Argentina. In folk medicine, Inga species are used to treat various diseases. The species Inga laurina is widely found in the Brazilian flora; however, there are few studies about its biological activity and chemical composition. The main purpose of this study was to identify and isolate the chemical constituents of Inga laurina barks and to evaluate the antifungal and cytotoxic activities. The total content of phenolics, proanthocyanidins, and flavonoids from the barks of Inga laurina were performed by spectrophotometric methods and the ethyl acetate (EAF) and n-butanol (BF) fractions showed the best results. Eleven compounds were identified in EAF by HPLC-ESI(−)-MS/MS, which showed good antifungal activity with MIC values of 23.4 and 46.8 μg mL−1, evaluated by broth microdilution method. Five new compounds of the genus Inga were isolated for the first time. Three of these compounds were isolated and reported on the literature for the first time: a proanthocyanidin B-type, gallocatechin-(4α→8)-4’-O-methylgallocatechin (XI) and two proanthocyanidins A-type, gallocatechin-(2→O→7,4→8)-4’-O-methylgallocatechin (XII) and gallocatechin-3-O-galloyl-(2→O→7,4→8)-4’-O-methylgallocatechin (XIII). The chemical study of the plant bark showed that this species is rich in phenolic compounds and it has great potential for the discovery of new bioactive compounds.

Research paper thumbnail of Antifungal and cytotoxicity activities and new proanthocyanidins isolated from the barks of Inga laurina (Sw.) Willd

Phytochemistry Letters

Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to nort... more Abstract The Inga genus comprises approximately 300 species that can be found from Mexico to northern Argentina. In folk medicine, Inga species are used to treat various diseases. The species Inga laurina is widely found in the Brazilian flora; however, there are few studies about its biological activity and chemical composition. The main purpose of this study was to identify and isolate the chemical constituents of Inga laurina barks and to evaluate the antifungal and cytotoxic activities. The total content of phenolics, proanthocyanidins, and flavonoids from the barks of Inga laurina were performed by spectrophotometric methods and the ethyl acetate (EAF) and n-butanol (BF) fractions showed the best results. Eleven compounds were identified in EAF by HPLC-ESI(−)-MS/MS, which showed good antifungal activity with MIC values of 23.4 and 46.8 μg mL−1, evaluated by broth microdilution method. Five new compounds of the genus Inga were isolated for the first time. Three of these compounds were isolated and reported on the literature for the first time: a proanthocyanidin B-type, gallocatechin-(4α→8)-4’-O-methylgallocatechin (XI) and two proanthocyanidins A-type, gallocatechin-(2→O→7,4→8)-4’-O-methylgallocatechin (XII) and gallocatechin-3-O-galloyl-(2→O→7,4→8)-4’-O-methylgallocatechin (XIII). The chemical study of the plant bark showed that this species is rich in phenolic compounds and it has great potential for the discovery of new bioactive compounds.

Research paper thumbnail of Antimicrobial activity, cytotoxicity and selectivity index of Banisteriopsis laevifolia (A. Juss.) B. Gates leaves

Industrial Crops and Products, 2016

Abstract Banisteriopsis laevifolia (A. Juss.) B. Gates (Malpighiaceae) is largely distributed in ... more Abstract Banisteriopsis laevifolia (A. Juss.) B. Gates (Malpighiaceae) is largely distributed in the cerrado Brazilian biome. In this study, phytochemical screening, antimicrobial and antifungal activities of leaves extract and partitions were evaluated. Phytochemical screening tests indicated the presence of flavonoids, terpenoids, phenols, sugar, steroids, triterpenoids, and tannins compounds. No alkaloids or nitrogenated compounds were found. Antioxidant, antimicrobial and antifungal activities were tested. The extract and partitions from the B. laevifolia leaves demonstrated relevant scavenging free radical DPPH effect. The crude extract and partitions inhibit bacteria growth with minimum inhibitory concentrations (MIC) below 400 mg L −1 for most oral microorganisms tested. Meanwhile, the antifungal activity proved to be very promising for the ethanolic extract and partitions (31–375 μg mL −1 ) against all yeasts tested. The antimicrobial activities results are very promising since the ethanol extract and the more active n -buthanol partition showed great selectivity (0.9–1.2; 0.1–0.7, respectively) against microorganisms and relatively low toxicity to Vero cells. Analysis by UHPLC-ESI–MS n from the most bioactive fractions (ethyl acetate and n -buthanol) permitted to identify ten phenolic compounds reported in the Banisteriopsis family that exert recognized antioxidant, antimicrobial and antifungal activity. Main secondary metabolites found were phenolic acids and flavonoid glycosides, mainly derivatives from quercetin and rutin. The biological activity results and MS analysis for the B.laevifolia leaves revealed that they have efficient antimicrobial agents, and contributed to knowledge of the genus.

Research paper thumbnail of Chemical Analysis and Study of Phenolics, Antioxidant Activity, and Antibacterial Effect of the Wood and Bark ofMaclura tinctoria(L.) D. Don ex Steud

Evidence-Based Complementary and Alternative Medicine, 2012

Maclura tinctoria(L.) D. Don ex Steud. has one of the highest qualities among the coefficients fo... more Maclura tinctoria(L.) D. Don ex Steud. has one of the highest qualities among the coefficients for Brazilian woods (up to 9.6) and resistance rates equivalent to Indian teak (Tectona grandis). In this study, the macromolecular constituents and total phenols compounds as well as the antioxidant and antibacterial activities of this wood were evaluated. Total phenols and proanthocyanidin levels were higher in wood when compared with bark levels. The antioxidant activity of wood extracts (IC50= 18.7 μg/mL) was more effective than that of bark extracts (IC50= 20.9 μg/mL). Wood and bark extracts revealed a high potential for inhibition of aerobic and anaerobic bacteria. The bark extracts were the most active (MIC from 20 to 60 μg/mL). Both antioxidant activity and high potential for bacteria inhibition turn these extracts promising for drug formulations, especially as antibacterial agent.

Research paper thumbnail of Looking for Unknown Molecules in Arabica and Robusta Coffee Brews by High Performance Liquid Chromatography Coupled to High Resolution Mass Spectrometry and Differentiating both Coffee Species by this Technique

The chemical composition of coffee brews originating from four varieties of Coffeaarabica cv. (Ye... more The chemical composition of coffee brews originating from four varieties of Coffeaarabica cv. (Yellow Catuai, Mundo Novo, Topazio and Acaia) and one variety of Coffeacanephora cv. (robusta) was evaluated by high performance liquid chromatography coupled to mass Spectrometry with high resolution ((HPLC/HRMS) at different roasting degrees. The results obtained in the present study demonstrate that there are still many unknown components that can influence the coffee flavor, in addition to those reported as such. They deserve been investigated. Further, more than different constituents, the different concentrations of the same constituents are crucial for distinguishing the flavor of arabica and robusta coffee brews.