Wisnu Nurcahyo | Universitas Gadjah Mada (Yogyakarta) (original) (raw)
Papers by Wisnu Nurcahyo
![Research paper thumbnail of Detection of Toxoplasma gondii in cat's internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin](https://attachments.academia-assets.com/108313126/thumbnails/1.jpg)
](Veterinary World, Sep 1, 2017
Několik roupů z cerstveho trusu dvou dospělých orangutannich samic bylo nalezeno v průběhu parazi... more Několik roupů z cerstveho trusu dvou dospělých orangutannich samic bylo nalezeno v průběhu parazitologicke studie v narodnim parku Gunung Leuser (Severni Sumatra, Indonesie) a okamžitě fixovano ve 40%alkoholu. Světelna a rastrovaci elektronova mikroskopie a molekularni analýzy ukazuji na nový druh a rod.
Communications Medicine
Background A recent dramatic surge in pet ownership has been observed across metropolitan areas i... more Background A recent dramatic surge in pet ownership has been observed across metropolitan areas in Asia. To date, there is a dearth of information on the risk associated with pet ownership for the transmission of parasites on a large scale in Asia, despite this continent giving rise to the largest burden of zoonotic infections worldwide. Methods We explored the nature and extent of zoonotic internal (endo-) and external (ecto-) parasites and arthropod-borne pathogens in 2381 client-owned dogs and cats living in metropolitan areas of eight countries in East and Southeast Asia using reliable diagnostic tests and then undertook extensive statistical analyses to define predictors of exposure to zoonotic pathogens. Results The estimated ORs for overall parasite infections are 1.35 [95% CIs 1.07;1.71] in young animals and 4.10 [1.50;11.2] in the animal group older than 15 years as compared with adult animals, 0.61 [0.48;0.77] in neutered animals as compared to unneutered animals, 0.36 [0....
The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given ... more The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups wit...
Biodiversitas Journal of Biological Diversity, 2020
Orangutans are in an extremely precarious state, with the Sumatran orangutan (Pongo abelii) criti... more Orangutans are in an extremely precarious state, with the Sumatran orangutan (Pongo abelii) critically endangered and the Bornean orangutan (Pongo pygmeaus) endangered with rapidly declining populations. There is only limited information available on orangutan parasites in general. Studies focusing on the parasites of orangutans in their natural habitat are even rarer, making any new information especially valuable. The most extensive record of orangutan parasites comes from a study which summarizes the data based only on standard coprology analyses often with not clear identification to the parasite species level. We would like to present SEM as important tools in parasites identification to the species level which have been used in recently described new pinworm species and identification of Ascaris sp. parasitizing Sumatran orangutan.
This study aims to determine the effective concentration of young fruit skin Calotropis procera (... more This study aims to determine the effective concentration of young fruit skin Calotropis procera (C. procera) in inhibiting the development of Haemonchus contortus worm eggs in vitro. The study was divided into 5 treatment groups consisting of 3 C. procera extracts treatment groups with concentrations: 2.5%, 3.5%, 4.5% of 0.2g / ml of the stock solution, a negative control group (-) using distilled water and a positive control group (+) using albendazole with concentration of 0.055%. The data were analyzed descriptively. The effective concentration of skin extracts from young C. procera (SEYCP) in inhibiting the development of eggs hatching worms Haemonchus contortus in vitro were: treated SEYCP concentration of 4.5% with a 88% inhibitory effect, SEYCP treatment in 2.5% and 3.5 % yielding inhibitory effect of 70.5% and 81% respectively.
Jurnal Veteriner, 2013
Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of t... more Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.
Rusa merupakansatwaliar yangbeberapaspesiesdiantaranyamerupakanrusa asli IndonesiamisalnyaRusaJaw... more Rusa merupakansatwaliar yangbeberapaspesiesdiantaranyamerupakanrusa asli IndonesiamisalnyaRusaJawa(Cervus timorensis),rusaSambar(Cervusunicofot}.rusaBawean (Axiskuhll)danMunljak(Muntiacusmuntjak).Mengingat populasinyadialamyangsemakinlerdesak dan punah.makaperlu diupayakanpenyelamatanmelaluikegiatanpenangkaranyangdapal diharapkankeunlungannyaA.nalisaekonompi elemakanrusadi Indonesiasulnuntukdiperkirdkan, karenamemangbelwnadapetemakanrusadiIndonesiaRusatemyalamemmkniilaitinggidibanding dengantemakumumnyakarenakeunggulanhasilpetemakanrusa.Bitadibandingkan denganhewanhewantemak. rusamempunyaikeunggulandari kualnasdaging.hargadagingrusadan harga velvet(tandukrusa).kemampuanmenkonverspiakan.produkski arkasperhektarlahandantenaga ke~ayangdiperlukanuntukmengelolapetemakan.DaribeberapakeunggulandiatascflSimpulkan bahwarusasebenamyamemilikipotensiyangsanga!besaruntukdi budidayakansebagaisalah satuallematifpilihanyangmendukungprogramagroforestry.
During our parasitological monitoring of wild and semi-wild orang-utans, throughout Sumatra and K... more During our parasitological monitoring of wild and semi-wild orang-utans, throughout Sumatra and Kalimantan, 16 different parasitic species were identified. Balantidium coli that could cause severe morbidity and fatalities had the highest prevalence observed. For molecular characterization, the region of ribosomal DNA gene cluster (ITS1, 5.8S, ITS2) was amplified, sequenced and compared. The NJ/MP/ML phylogenetic analysis included comparison of data for order Vestibuliferida, families Balantidiidae (genera Balantidium, Neobalantidium) and Pycnotrichidae (genus Buxtonella). Data from the relative genus Troglodytella (Troglodytellidae, Entodiniomorphida) were used as an out-group. Our samples clustered in separate clades, corresponding to the different host origins. The progress in the identification of biologically active plant extracts with potential anti-parasitic properties (treating of balantidiasis) and their applications was also discussed.
Jurnal Pengabdian Pada Masyarakat, 2020
INNOVATION OF INSTANT MOCAF PANCAKE PREMIX FLOUR AND PANCAKE OF “GAMA PANTELO” IN GEBANG HAMLET, ... more INNOVATION OF INSTANT MOCAF PANCAKE PREMIX FLOUR AND PANCAKE OF “GAMA PANTELO” IN GEBANG HAMLET, KEMIRI VILLAGE, TANJUNGSARI GUNUNGKIDUL REGENCY. The purpose of this activity is to empower the Gebang hamlet farmer women's group, Kemiri village, Tanjungsari District Gunungkidul Regency in optimizing mocaf products so that they have time keep the old and not undergo physical and chemical changes, practical and easy to process in the form of Instant Mocaf Flour Pancake Mocaf “Gama pantelo” as a snack or dessert that can be eaten at any time. The method of activity is done by demonstration and hands-on practice. “Gama Pantelo” Instant Premix Pancake Flour is made by pouring mocaf flour, refined sugar, baking powder, milk powder, and vanilli in a basin by passing it on a sieve to get a homogeneous powder size, then stirring it evenly and then mixing the instant mocaf pancake flour in aluminum foil zipperlock packaging. Pancakes are made with instant premix mocaf pancakes mixed with 1...
Haemonchosis disebabkan oleh cacing nematoda Haemonchus contortus,dengan karakteristik menghisap ... more Haemonchosis disebabkan oleh cacing nematoda Haemonchus contortus,dengan karakteristik menghisap darah, hidup di abomasum ruminansia dan dapat menyebabkan kerugian ekonomi yang tinggi di seluruh dunia. Parasit ini dapat menyebabkan anemia, edema dan jika tidak ditangani serius dapat menimbulkan kematian. Penanganan dan pengendalian menggunakan anthelmintika kimia yang berspektrum luas dapat menjadi ancaman serius kesehatan konsumen akibat timbulnya resistensi dan residu jika digunakan secara terus menerus dalam waktu yang lama.. Kandungan tannin dalam daun bambu berpotensi sebagai agen anthelmintik. Penelitian ini bertujuan untuk melihat potensi anthelmintic daun bambu terhadap tingkat mortalitas cacing dewasa secara in vitro dan keberadaan profil protein dalam H. contortus. Cacing H. contortus diperoleh dari abomasum kambing bligon terinfeksi H. contortus yang dipotong di RPH Sleman. H. contortus direndam pada ekstrak larutan daun bambu 0,1% dan 1%. Isolasi protein H. Contortus unt...
Emerging Infectious Diseases, 2020
THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity”, 2020
Jurnal Sain Veteriner, Dec 1, 2017
Journal of the Indonesian Tropical Animal Agriculture, 2019
The study aimed at investigating the population, the protein profile and the ultrastructure of ad... more The study aimed at investigating the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract. Fifty domestic female chickens of 6 weeks of age were assigned to 5 groups. Group A (negative control) was not given any treatment and any drug. Groups B, C and D were given the treatment at the doses of 26 mg/mL, 53 mg/mL and 79 mg/mL, respectively. Group E (positive control) was given Pyrantel®. Necropsy was conducted to all of the chickens 14 days after the treatment. Adult worms were collected and counted. The worms used in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Scanning Electron Microscopy (SEM) were those collected from the jejunum of the chickens in the groups A, B and C. The biggest number of the worms was found in the jejunum. The results of electrophoresis showed that the dose 53 mg/mL gave fewer protein bands than the negative control (21:12 ratio...
AIDS Research and Human Retroviruses, 2001
To acquire new knowledge about the genetic diversity and potential impact on vaccine strategies o... more To acquire new knowledge about the genetic diversity and potential impact on vaccine strategies of HIV-1 subtype C in South Africa, we have characterized the vif, vpr, and vpu genes of 15 isolates. Phylogenetic analysis of the genomic fragment encompassing these genes revealed subtype C subclusters, suggesting close relatedness with subtype C strains from other geographic locations and excluded isolation of South African strains. The putative T155 phosphorylation site in the C terminal of Vif was absent in all subtype C sequences. Variation in the predicted amino acid sequences of the three genes further showed strong correlation with other subtype C sequences.
Veterinary World, Sep 1, 2017
Několik roupů z cerstveho trusu dvou dospělých orangutannich samic bylo nalezeno v průběhu parazi... more Několik roupů z cerstveho trusu dvou dospělých orangutannich samic bylo nalezeno v průběhu parazitologicke studie v narodnim parku Gunung Leuser (Severni Sumatra, Indonesie) a okamžitě fixovano ve 40%alkoholu. Světelna a rastrovaci elektronova mikroskopie a molekularni analýzy ukazuji na nový druh a rod.
Communications Medicine
Background A recent dramatic surge in pet ownership has been observed across metropolitan areas i... more Background A recent dramatic surge in pet ownership has been observed across metropolitan areas in Asia. To date, there is a dearth of information on the risk associated with pet ownership for the transmission of parasites on a large scale in Asia, despite this continent giving rise to the largest burden of zoonotic infections worldwide. Methods We explored the nature and extent of zoonotic internal (endo-) and external (ecto-) parasites and arthropod-borne pathogens in 2381 client-owned dogs and cats living in metropolitan areas of eight countries in East and Southeast Asia using reliable diagnostic tests and then undertook extensive statistical analyses to define predictors of exposure to zoonotic pathogens. Results The estimated ORs for overall parasite infections are 1.35 [95% CIs 1.07;1.71] in young animals and 4.10 [1.50;11.2] in the animal group older than 15 years as compared with adult animals, 0.61 [0.48;0.77] in neutered animals as compared to unneutered animals, 0.36 [0....
The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given ... more The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups wit...
Biodiversitas Journal of Biological Diversity, 2020
Orangutans are in an extremely precarious state, with the Sumatran orangutan (Pongo abelii) criti... more Orangutans are in an extremely precarious state, with the Sumatran orangutan (Pongo abelii) critically endangered and the Bornean orangutan (Pongo pygmeaus) endangered with rapidly declining populations. There is only limited information available on orangutan parasites in general. Studies focusing on the parasites of orangutans in their natural habitat are even rarer, making any new information especially valuable. The most extensive record of orangutan parasites comes from a study which summarizes the data based only on standard coprology analyses often with not clear identification to the parasite species level. We would like to present SEM as important tools in parasites identification to the species level which have been used in recently described new pinworm species and identification of Ascaris sp. parasitizing Sumatran orangutan.
This study aims to determine the effective concentration of young fruit skin Calotropis procera (... more This study aims to determine the effective concentration of young fruit skin Calotropis procera (C. procera) in inhibiting the development of Haemonchus contortus worm eggs in vitro. The study was divided into 5 treatment groups consisting of 3 C. procera extracts treatment groups with concentrations: 2.5%, 3.5%, 4.5% of 0.2g / ml of the stock solution, a negative control group (-) using distilled water and a positive control group (+) using albendazole with concentration of 0.055%. The data were analyzed descriptively. The effective concentration of skin extracts from young C. procera (SEYCP) in inhibiting the development of eggs hatching worms Haemonchus contortus in vitro were: treated SEYCP concentration of 4.5% with a 88% inhibitory effect, SEYCP treatment in 2.5% and 3.5 % yielding inhibitory effect of 70.5% and 81% respectively.
Jurnal Veteriner, 2013
Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of t... more Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.
Rusa merupakansatwaliar yangbeberapaspesiesdiantaranyamerupakanrusa asli IndonesiamisalnyaRusaJaw... more Rusa merupakansatwaliar yangbeberapaspesiesdiantaranyamerupakanrusa asli IndonesiamisalnyaRusaJawa(Cervus timorensis),rusaSambar(Cervusunicofot}.rusaBawean (Axiskuhll)danMunljak(Muntiacusmuntjak).Mengingat populasinyadialamyangsemakinlerdesak dan punah.makaperlu diupayakanpenyelamatanmelaluikegiatanpenangkaranyangdapal diharapkankeunlungannyaA.nalisaekonompi elemakanrusadi Indonesiasulnuntukdiperkirdkan, karenamemangbelwnadapetemakanrusadiIndonesiaRusatemyalamemmkniilaitinggidibanding dengantemakumumnyakarenakeunggulanhasilpetemakanrusa.Bitadibandingkan denganhewanhewantemak. rusamempunyaikeunggulandari kualnasdaging.hargadagingrusadan harga velvet(tandukrusa).kemampuanmenkonverspiakan.produkski arkasperhektarlahandantenaga ke~ayangdiperlukanuntukmengelolapetemakan.DaribeberapakeunggulandiatascflSimpulkan bahwarusasebenamyamemilikipotensiyangsanga!besaruntukdi budidayakansebagaisalah satuallematifpilihanyangmendukungprogramagroforestry.
During our parasitological monitoring of wild and semi-wild orang-utans, throughout Sumatra and K... more During our parasitological monitoring of wild and semi-wild orang-utans, throughout Sumatra and Kalimantan, 16 different parasitic species were identified. Balantidium coli that could cause severe morbidity and fatalities had the highest prevalence observed. For molecular characterization, the region of ribosomal DNA gene cluster (ITS1, 5.8S, ITS2) was amplified, sequenced and compared. The NJ/MP/ML phylogenetic analysis included comparison of data for order Vestibuliferida, families Balantidiidae (genera Balantidium, Neobalantidium) and Pycnotrichidae (genus Buxtonella). Data from the relative genus Troglodytella (Troglodytellidae, Entodiniomorphida) were used as an out-group. Our samples clustered in separate clades, corresponding to the different host origins. The progress in the identification of biologically active plant extracts with potential anti-parasitic properties (treating of balantidiasis) and their applications was also discussed.
Jurnal Pengabdian Pada Masyarakat, 2020
INNOVATION OF INSTANT MOCAF PANCAKE PREMIX FLOUR AND PANCAKE OF “GAMA PANTELO” IN GEBANG HAMLET, ... more INNOVATION OF INSTANT MOCAF PANCAKE PREMIX FLOUR AND PANCAKE OF “GAMA PANTELO” IN GEBANG HAMLET, KEMIRI VILLAGE, TANJUNGSARI GUNUNGKIDUL REGENCY. The purpose of this activity is to empower the Gebang hamlet farmer women's group, Kemiri village, Tanjungsari District Gunungkidul Regency in optimizing mocaf products so that they have time keep the old and not undergo physical and chemical changes, practical and easy to process in the form of Instant Mocaf Flour Pancake Mocaf “Gama pantelo” as a snack or dessert that can be eaten at any time. The method of activity is done by demonstration and hands-on practice. “Gama Pantelo” Instant Premix Pancake Flour is made by pouring mocaf flour, refined sugar, baking powder, milk powder, and vanilli in a basin by passing it on a sieve to get a homogeneous powder size, then stirring it evenly and then mixing the instant mocaf pancake flour in aluminum foil zipperlock packaging. Pancakes are made with instant premix mocaf pancakes mixed with 1...
Haemonchosis disebabkan oleh cacing nematoda Haemonchus contortus,dengan karakteristik menghisap ... more Haemonchosis disebabkan oleh cacing nematoda Haemonchus contortus,dengan karakteristik menghisap darah, hidup di abomasum ruminansia dan dapat menyebabkan kerugian ekonomi yang tinggi di seluruh dunia. Parasit ini dapat menyebabkan anemia, edema dan jika tidak ditangani serius dapat menimbulkan kematian. Penanganan dan pengendalian menggunakan anthelmintika kimia yang berspektrum luas dapat menjadi ancaman serius kesehatan konsumen akibat timbulnya resistensi dan residu jika digunakan secara terus menerus dalam waktu yang lama.. Kandungan tannin dalam daun bambu berpotensi sebagai agen anthelmintik. Penelitian ini bertujuan untuk melihat potensi anthelmintic daun bambu terhadap tingkat mortalitas cacing dewasa secara in vitro dan keberadaan profil protein dalam H. contortus. Cacing H. contortus diperoleh dari abomasum kambing bligon terinfeksi H. contortus yang dipotong di RPH Sleman. H. contortus direndam pada ekstrak larutan daun bambu 0,1% dan 1%. Isolasi protein H. Contortus unt...
Emerging Infectious Diseases, 2020
THE 6TH INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCE ICBS 2019: “Biodiversity as a Cornerstone for Embracing Future Humanity”, 2020
Jurnal Sain Veteriner, Dec 1, 2017
Journal of the Indonesian Tropical Animal Agriculture, 2019
The study aimed at investigating the population, the protein profile and the ultrastructure of ad... more The study aimed at investigating the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract. Fifty domestic female chickens of 6 weeks of age were assigned to 5 groups. Group A (negative control) was not given any treatment and any drug. Groups B, C and D were given the treatment at the doses of 26 mg/mL, 53 mg/mL and 79 mg/mL, respectively. Group E (positive control) was given Pyrantel®. Necropsy was conducted to all of the chickens 14 days after the treatment. Adult worms were collected and counted. The worms used in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Scanning Electron Microscopy (SEM) were those collected from the jejunum of the chickens in the groups A, B and C. The biggest number of the worms was found in the jejunum. The results of electrophoresis showed that the dose 53 mg/mL gave fewer protein bands than the negative control (21:12 ratio...
AIDS Research and Human Retroviruses, 2001
To acquire new knowledge about the genetic diversity and potential impact on vaccine strategies o... more To acquire new knowledge about the genetic diversity and potential impact on vaccine strategies of HIV-1 subtype C in South Africa, we have characterized the vif, vpr, and vpu genes of 15 isolates. Phylogenetic analysis of the genomic fragment encompassing these genes revealed subtype C subclusters, suggesting close relatedness with subtype C strains from other geographic locations and excluded isolation of South African strains. The putative T155 phosphorylation site in the C terminal of Vif was absent in all subtype C sequences. Variation in the predicted amino acid sequences of the three genes further showed strong correlation with other subtype C sequences.