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Research paper thumbnail of The Effect of Erythropoetin Administration in Experimental Burns Wound Healing : An Animal Study

The Effect of Erythropoietin Administration in Experimental Burns Wound Healing: An Animal Stud... more The Effect of Erythropoietin Administration in Experimental Burns Wound Healing:
An Animal Study

ABSTRACT

BACKGROUNDS: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its all-tissue-protective pleiotropic properties. EPO interacts with vascular endothelial growth factor (VEGF) and stimulates endothelial cell mitosis and motility in neo-angiogenesis; thus it may of importance in the complex phenomenon of wound healing. The purpose of this study is to investigate the effect of EPO in experimental burn wounds healing. METHODS: Fifteen healthy Sprague-Dawley, strain of Rattus Novergicus weighing 300-350 grams, were prepared to achieve deep dermal burns. Animals were randomized to receive either low-dose EPO injection (600 IU/mL), high-dose EPO injection (3000 IU/mL) or nothing (control group). After 14 days of observations, a quantitative assessment of wound healing was determined by percentage of wound closure and epithelialization using Analyzing Digital Images® Software. And qualitative assessment was done to evaluate the score of histopathological changes in wound healing. RESULTS: The size of the wound area and re-epithelialization rate percentage was determined on Day-0, Day-5, Day-10 and Day-14. The average of raw surface areas measurement (p value: 0.012 in day-5; 0.009 in day-10 and 0.000 in day-14) and healing percentage of the lesions (p value: 0.011 in day-5; 0.016 in day-10 and 0.010 in day-14) were significantly best in the low-dose EPO group compared to the control group and high-dose EPO group. The histopathology evaluation revealed that the highest score for re-epithelialization, granulation tissue and neo-angiogenesis were achieved by the low-dose EPO injection group than in both control and high-dose EPO injection groups. CONCLUSIONS: In this animal study using Sprague-Dawley rats, Recombinant Human EPO (rHuEPO) injection administration prompted the evidences of improved re-epithelialization and wound healing process of the skin caused by deep dermal burns. These findings may lead to a new therapeutic approach to improve the clinical outcomes for the management of burns wound healing.

KEYWORDS: burns, erythropoietin, wound healing

Papers by Afriyanti Sandhi

Research paper thumbnail of Microsurgery Technique in the Foot Reconstruction of Soft Tissue Defects: A Review of 4 Cases

Jurnal Plastik Rekonstruksi, 1970

The restoration of an intact covering is the primary surgical requisite following soft tissue def... more The restoration of an intact covering is the primary surgical requisite following soft tissue defects in the foot because deep healing can be no better than the surface covering. Soft tissue defects that expose underlying bones, joints, and tendons pose challenging problems and require a free tissue transfer for a successful reconstruction.Total of 4 flaps in the foot was performed between February 2009 to February 2010. We reconstructed soft tissue defects in the foot in 4 patients using 3 free anterolateral thigh (ALT) flaps and 1 free radial forearm (RF) flap. Trauma was the commonest cause in our patients.Free ALT and RF flaps provided stable and durable long-term wound cover in all patients. Complications were few and manageable.Free tissue transfer has become commonplace in many centers around the world. The numerous advantages include stable wound coverage; improve aesthetic and functional outcomes, and minimal donor site morbidity. In our experience, we found that the using ...

Research paper thumbnail of Sole Reconstruction Using Anterolateral Thigh Free Flap: A Review of 4 Cases

Jurnal Plastik Rekonstruksi, 1970

Background: The ideal technique for sole reconstruction should meet such requisite as a durable a... more Background: The ideal technique for sole reconstruction should meet such requisite as a durable and comfortable weight-bearing surface, solid anchoring to deep tissue for resistance to shear force and adequate protective sensation. The anterolateral thigh (ALT) perforator free flap provides a large, pliable skin island and sufficient bulk, allowing three-dimensional tailoring to complex defects. The present article is about our experience of sole reconstruction ALT perforator free flap.Patient and Method: Total of 4 flaps in the foot defects was performed in February 2009 to December 2012. We reconstructed soft tissue defects in the sole using ALT free flaps, the complications, aesthetic and function results were monitored.Result: ALT perforator free flap provided stable and durable long-term wound cover in all patients, resulting in early rehabilitation and function outcomes were completely achieved. Complications were few and manageable in all cases.Summary: ALT perforator free fl...

Research paper thumbnail of Acromiocervical Flap As One of the Modality To Reconstruct Post-Burn Neck Contracture: A Case Report

Jurnal Plastik Rekonstruksi, 1970

Luka bakar yang mengenai daerah leher merupakan kasus yang sulit dan sering dijumpai. Luka bakar ... more Luka bakar yang mengenai daerah leher merupakan kasus yang sulit dan sering dijumpai. Luka bakar leher mengganggu fungsi gerak dan penampilan akibat kontraktur dan deformitas yang serius pada wajah. Hasil rekonstruksi yang memuaskan untuk kasus kontraktur leher merupakan tantangan bagi seorang spesialis bedah plastik. Beberapa teknik rekonstruksi kontraktur leher telah banyak dipubilkasikan, skin graft, ap local, ap regional maupun "free ap". Pasien dan Metode: Kontraktur akibat luka bakar leher pada kasus ini telah ditangani oleh dua orang spesialis bedah plastik di dua rumah sakit yang berbeda dengan menggunakan modalitas skin graft. Namun di kemudian hari timbul masalah kontraktur leher ulang yang mengganggu fungsi bicara, makan, minum, dan penampilan. Kami melaporkan penggunaan acromiocervical ap pada kasus tersebut dengan harapan dapat mencegah timbulnya kontraktur ulang. Hasil: Rekonstruksi kontraktur pada luka bakar terutama daerah leher untuk mencegah terjadinya kontraktur ulang dengan menggunakan acromiocervical ap merupakan salah satu pilihan karena mudah dikerjakan, reliable dan memberikan hasil secara kosmetik lebih baik. Ringkasan: Skin graft untuk rekonstruksi kontraktur akibat luka bakar leher sebaiknya dipertimbangkan betul manfaatnya karena pengerjaannya tidak mudah, perawatan pasca operasinya tidak sepenuhnya dapat dikuasai karena memerlukan program rehabilitasi yang komprehensif walaupun telah menggunakan bidai leher sekalipun.

Research paper thumbnail of Latar Belakang : Pemulihan penutup yang menempel pada kulit adalah syarat utama dari penu

tupan defek pada jaringan lunak pada kaki, sebab proses penyembuhan luka tidak lebih baik dari pe... more tupan defek pada jaringan lunak pada kaki, sebab proses penyembuhan luka tidak lebih baik dari penutupan permukaan luka. Jaringan lunak yang terekpos dasarnya tulang, persendian, atau tendon menimbulkan masalah baru dan memerlukan transfer jaringan lepas untuk rekonstruksi yang maksimal. Metode: Terdapat 4 total operasi flap kaki dalam selama Februari 2009-Februari 2010. Rekonstruksi defek pada jaringan lunak kaki pada 3 pasien menggunakan tiga flap bebas bagian anterolateral paha dan flap bebas lengan bawah radialis. Hasil: Flap bebas anterolateral paha dan lengan bawah radialis memberikan hasil yang stabil dan menutup luka berdurasi jangka lama pada semua pasien. Komplikasi yang ditemukan sedikit. Kesimpulan: Transfer jaringan bebas menjadi pilihan utama pada pusat-pusat kesehatan di dunia. Banyaknya keuntungan seperti penutupan luka yang lebih stabil, memperbaiki hasil akhir secara fungsional dan estetik, dan menekan morbiditas dari lokasi donor.Berdasarkan pelaopran kasus ini, p...

Research paper thumbnail of The Effect of Erythropoietin Administration in Experimental Burns Wound Healing: An Animal Study

Background: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its al... more Background: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its all-tissue-protective pleiotropic properties. The purpose of this study is to investigate the effect of EPO in experimental burn wounds healing. Methods: Fifteen healthy Sprague-Dawley, strain of Rattus Novergicus weighing 300-350 grams, were prepared to achieve deep dermal burns. Animals were randomized to receive either low-dose EPO injection (600 IU/mL), highdose EPO injection (3000 IU/mL) or nothing (control group). After 14 days of observations, quantitative and qualitative assessments of wound healing was determined. Results: The size of the wound area and re-epithelialization rate percentage was determined on Day-0, Day-5, Day-10, and Day-14. The average of raw surface areas measurement (p value: 0.012 in day-5; 0.009 in day-10 and 0.000 in day-14) and healing percentage of the lesions (p value: 0.011 in day-5; 0.016 in day-10 and 0.010 in day-14) were significantly best in the low-dose EPO grup compared to the control group and high-dose EPO grup. The histopathology evaluation revealed that the highest score for for re-epithelialization, granulation tissue and neo-angiogenesis were achieved by the low-dose EPO injection group than in both control and high-dose EPO injection groups. Conclusion: In this animal study using Sprague-Dawley rats, Recombinant Human EPO (rHuEPO) injection administration prompted the evidences of improved re-epithelialization and wound healing process of the skin caused by deep dermal burns. These findings may lead to a new therapeutic approach to improve the clinical outcomes for the management of burns wound healing.

Research paper thumbnail of The Effect of Erythropoetin Administration in Experimental Burns Wound Healing : An Animal Study

The Effect of Erythropoietin Administration in Experimental Burns Wound Healing: An Animal Stud... more The Effect of Erythropoietin Administration in Experimental Burns Wound Healing:
An Animal Study

ABSTRACT

BACKGROUNDS: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its all-tissue-protective pleiotropic properties. EPO interacts with vascular endothelial growth factor (VEGF) and stimulates endothelial cell mitosis and motility in neo-angiogenesis; thus it may of importance in the complex phenomenon of wound healing. The purpose of this study is to investigate the effect of EPO in experimental burn wounds healing. METHODS: Fifteen healthy Sprague-Dawley, strain of Rattus Novergicus weighing 300-350 grams, were prepared to achieve deep dermal burns. Animals were randomized to receive either low-dose EPO injection (600 IU/mL), high-dose EPO injection (3000 IU/mL) or nothing (control group). After 14 days of observations, a quantitative assessment of wound healing was determined by percentage of wound closure and epithelialization using Analyzing Digital Images® Software. And qualitative assessment was done to evaluate the score of histopathological changes in wound healing. RESULTS: The size of the wound area and re-epithelialization rate percentage was determined on Day-0, Day-5, Day-10 and Day-14. The average of raw surface areas measurement (p value: 0.012 in day-5; 0.009 in day-10 and 0.000 in day-14) and healing percentage of the lesions (p value: 0.011 in day-5; 0.016 in day-10 and 0.010 in day-14) were significantly best in the low-dose EPO group compared to the control group and high-dose EPO group. The histopathology evaluation revealed that the highest score for re-epithelialization, granulation tissue and neo-angiogenesis were achieved by the low-dose EPO injection group than in both control and high-dose EPO injection groups. CONCLUSIONS: In this animal study using Sprague-Dawley rats, Recombinant Human EPO (rHuEPO) injection administration prompted the evidences of improved re-epithelialization and wound healing process of the skin caused by deep dermal burns. These findings may lead to a new therapeutic approach to improve the clinical outcomes for the management of burns wound healing.

KEYWORDS: burns, erythropoietin, wound healing

Research paper thumbnail of Microsurgery Technique in the Foot Reconstruction of Soft Tissue Defects: A Review of 4 Cases

Jurnal Plastik Rekonstruksi, 1970

The restoration of an intact covering is the primary surgical requisite following soft tissue def... more The restoration of an intact covering is the primary surgical requisite following soft tissue defects in the foot because deep healing can be no better than the surface covering. Soft tissue defects that expose underlying bones, joints, and tendons pose challenging problems and require a free tissue transfer for a successful reconstruction.Total of 4 flaps in the foot was performed between February 2009 to February 2010. We reconstructed soft tissue defects in the foot in 4 patients using 3 free anterolateral thigh (ALT) flaps and 1 free radial forearm (RF) flap. Trauma was the commonest cause in our patients.Free ALT and RF flaps provided stable and durable long-term wound cover in all patients. Complications were few and manageable.Free tissue transfer has become commonplace in many centers around the world. The numerous advantages include stable wound coverage; improve aesthetic and functional outcomes, and minimal donor site morbidity. In our experience, we found that the using ...

Research paper thumbnail of Sole Reconstruction Using Anterolateral Thigh Free Flap: A Review of 4 Cases

Jurnal Plastik Rekonstruksi, 1970

Background: The ideal technique for sole reconstruction should meet such requisite as a durable a... more Background: The ideal technique for sole reconstruction should meet such requisite as a durable and comfortable weight-bearing surface, solid anchoring to deep tissue for resistance to shear force and adequate protective sensation. The anterolateral thigh (ALT) perforator free flap provides a large, pliable skin island and sufficient bulk, allowing three-dimensional tailoring to complex defects. The present article is about our experience of sole reconstruction ALT perforator free flap.Patient and Method: Total of 4 flaps in the foot defects was performed in February 2009 to December 2012. We reconstructed soft tissue defects in the sole using ALT free flaps, the complications, aesthetic and function results were monitored.Result: ALT perforator free flap provided stable and durable long-term wound cover in all patients, resulting in early rehabilitation and function outcomes were completely achieved. Complications were few and manageable in all cases.Summary: ALT perforator free fl...

Research paper thumbnail of Acromiocervical Flap As One of the Modality To Reconstruct Post-Burn Neck Contracture: A Case Report

Jurnal Plastik Rekonstruksi, 1970

Luka bakar yang mengenai daerah leher merupakan kasus yang sulit dan sering dijumpai. Luka bakar ... more Luka bakar yang mengenai daerah leher merupakan kasus yang sulit dan sering dijumpai. Luka bakar leher mengganggu fungsi gerak dan penampilan akibat kontraktur dan deformitas yang serius pada wajah. Hasil rekonstruksi yang memuaskan untuk kasus kontraktur leher merupakan tantangan bagi seorang spesialis bedah plastik. Beberapa teknik rekonstruksi kontraktur leher telah banyak dipubilkasikan, skin graft, ap local, ap regional maupun "free ap". Pasien dan Metode: Kontraktur akibat luka bakar leher pada kasus ini telah ditangani oleh dua orang spesialis bedah plastik di dua rumah sakit yang berbeda dengan menggunakan modalitas skin graft. Namun di kemudian hari timbul masalah kontraktur leher ulang yang mengganggu fungsi bicara, makan, minum, dan penampilan. Kami melaporkan penggunaan acromiocervical ap pada kasus tersebut dengan harapan dapat mencegah timbulnya kontraktur ulang. Hasil: Rekonstruksi kontraktur pada luka bakar terutama daerah leher untuk mencegah terjadinya kontraktur ulang dengan menggunakan acromiocervical ap merupakan salah satu pilihan karena mudah dikerjakan, reliable dan memberikan hasil secara kosmetik lebih baik. Ringkasan: Skin graft untuk rekonstruksi kontraktur akibat luka bakar leher sebaiknya dipertimbangkan betul manfaatnya karena pengerjaannya tidak mudah, perawatan pasca operasinya tidak sepenuhnya dapat dikuasai karena memerlukan program rehabilitasi yang komprehensif walaupun telah menggunakan bidai leher sekalipun.

Research paper thumbnail of Latar Belakang : Pemulihan penutup yang menempel pada kulit adalah syarat utama dari penu

tupan defek pada jaringan lunak pada kaki, sebab proses penyembuhan luka tidak lebih baik dari pe... more tupan defek pada jaringan lunak pada kaki, sebab proses penyembuhan luka tidak lebih baik dari penutupan permukaan luka. Jaringan lunak yang terekpos dasarnya tulang, persendian, atau tendon menimbulkan masalah baru dan memerlukan transfer jaringan lepas untuk rekonstruksi yang maksimal. Metode: Terdapat 4 total operasi flap kaki dalam selama Februari 2009-Februari 2010. Rekonstruksi defek pada jaringan lunak kaki pada 3 pasien menggunakan tiga flap bebas bagian anterolateral paha dan flap bebas lengan bawah radialis. Hasil: Flap bebas anterolateral paha dan lengan bawah radialis memberikan hasil yang stabil dan menutup luka berdurasi jangka lama pada semua pasien. Komplikasi yang ditemukan sedikit. Kesimpulan: Transfer jaringan bebas menjadi pilihan utama pada pusat-pusat kesehatan di dunia. Banyaknya keuntungan seperti penutupan luka yang lebih stabil, memperbaiki hasil akhir secara fungsional dan estetik, dan menekan morbiditas dari lokasi donor.Berdasarkan pelaopran kasus ini, p...

Research paper thumbnail of The Effect of Erythropoietin Administration in Experimental Burns Wound Healing: An Animal Study

Background: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its al... more Background: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its all-tissue-protective pleiotropic properties. The purpose of this study is to investigate the effect of EPO in experimental burn wounds healing. Methods: Fifteen healthy Sprague-Dawley, strain of Rattus Novergicus weighing 300-350 grams, were prepared to achieve deep dermal burns. Animals were randomized to receive either low-dose EPO injection (600 IU/mL), highdose EPO injection (3000 IU/mL) or nothing (control group). After 14 days of observations, quantitative and qualitative assessments of wound healing was determined. Results: The size of the wound area and re-epithelialization rate percentage was determined on Day-0, Day-5, Day-10, and Day-14. The average of raw surface areas measurement (p value: 0.012 in day-5; 0.009 in day-10 and 0.000 in day-14) and healing percentage of the lesions (p value: 0.011 in day-5; 0.016 in day-10 and 0.010 in day-14) were significantly best in the low-dose EPO grup compared to the control group and high-dose EPO grup. The histopathology evaluation revealed that the highest score for for re-epithelialization, granulation tissue and neo-angiogenesis were achieved by the low-dose EPO injection group than in both control and high-dose EPO injection groups. Conclusion: In this animal study using Sprague-Dawley rats, Recombinant Human EPO (rHuEPO) injection administration prompted the evidences of improved re-epithelialization and wound healing process of the skin caused by deep dermal burns. These findings may lead to a new therapeutic approach to improve the clinical outcomes for the management of burns wound healing.