Intercellular signaling via cyclic GMP diffusion through gap junctions restarts meiosis in mouse ovarian follicles (original) (raw)
ADS
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- Egbert, Jeremy R. ;
- Norris, Rachael P. ;
- Lampe, Paul D. ;
- Nikolaev, Viacheslav O. ;
- Thunemann, Martin ;
- Wen, Lai ;
- Feil, Robert ;
- Jaffe, Laurinda A.
Abstract
By imaging cyclic GMP (cGMP) in live ovarian follicles from mice, we show how luteinizing hormone signaling in the follicle periphery results in a rapid decrease in cGMP in the oocyte, thus reinitiating meiosis. Luteinizing hormone signaling lowers cGMP in the outer cells of the follicle, then cGMP in the oocyte decreases as a consequence of diffusion through gap junctions. These findings demonstrate directly that a physiological signal initiated by a stimulus in one region of an intact tissue can travel across many layers of cells via cyclic nucleotide diffusion through gap junctions.
Publication:
Proceedings of the National Academy of Science
Pub Date:
April 2015
DOI:
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