Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork (original) (raw)

ADS

;

• Higgins, Jenny L. ;
• Mannion, Patrick C. ;
• Tamkun, Michael M. ;
• Krapf, Diego

Abstract

A broad range of membrane proteins display anomalous diffusion on the cell surface. Different methods provide evidence for obstructed subdiffusion and diffusion on a fractal space, but the underlying structure inducing anomalous diffusion has never been visualized because of experimental challenges. We addressed this problem by imaging the cortical actin at high resolution while simultaneously tracking individual membrane proteins in live mammalian cells. Our data confirm that actin introduces barriers leading to compartmentalization of the plasma membrane and that membrane proteins are transiently confined within actin fences. Furthermore, superresolution imaging shows that the cortical actin is organized into a self-similar meshwork. These results present a hierarchical nanoscale picture of the plasma membrane.

Publication:

Physical Review X

Pub Date:

January 2017

DOI:

10.1103/PhysRevX.7.011031

10.48550/arXiv.1702.03997

arXiv:

arXiv:1702.03997

Bibcode:

2017PhRvX...7a1031S

Keywords:

• Physics - Biological Physics;
• Condensed Matter - Statistical Mechanics

E-Print:

Phys. Rev. X 7, 011031 (2017)