Chemical synthesis rewriting of a bacterial genome to achieve design flexibility and biological functionality (original) (raw)
ADS
;
- Del Medico, Luca ;
- Wölfle, Alexander ;
- Schächle, Philipp ;
- Bucher, Yves ;
- Appert, Donat ;
- Tschan, Flavia ;
- Flores-Tinoco, Carlos E. ;
- van Kooten, Mariëlle ;
- Guennoun, Rym ;
- Deutsch, Samuel ;
- Christen, Matthias ;
- Christen, Beat
Abstract
The fundamental biological functions of a living cell are stored within the DNA sequence of its genome. Classical genetic approaches dissect the functioning of biological systems by analyzing individual genes, yet uncovering the essential gene set of an organism has remained very challenging. It is argued that the rewriting of entire genomes through the process of chemical synthesis provides a powerful and complementary research concept to understand how essential functions are programed into genomes.
Publication:
Proceedings of the National Academy of Science
Pub Date:
April 2019
DOI:
Bibcode: