marys Booh | Universiti Teknologi Mara (original) (raw)

Papers by marys Booh

Article history: Received 23 July 2015 Accepted 25 August 2015 Available online 5 September 2015

This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus m... more This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus musculus. The daily intake of Cosmos caudatus was evaluated in determining its effects on male fertility. Four different concentrations of the crude ethanolic extracts which were 50 mg/kg, 125 mg/kg, 500 mg/kg and 1000 mg/kg, respectively were administered for 28 consecutive days. Sperm analyses includes sperm count, total sperm motility, sperm viability and sperm morphology were carried out using standard procedures outlined by WHO 2010. G3 group gives the highest mean percentage for change in body weight (9.60 gm), total sperm motility (56%) and normal sperm morphology count (62.8 sperm) as compared to the rest the group treatments. For the sperm count and sperm viability, G4 group showed the highest data; 56.5 million sperm/ml and 59.53%, respectively. The lowest data of the change in body weight and normal sperm morphology count of mice from G1 group which are 6.43 gm and 58.6 sperm, ...

Advances in Environmental Biology, 2015

Averrhoa bilimbi (AB), Cosmos caudatus (CC) and Pereskia bleo (PB) are among the local plants in ... more Averrhoa bilimbi (AB), Cosmos caudatus (CC) and Pereskia bleo (PB) are among the local plants in Malaysia enriched with plant secondary compounds scientifically proven to contain high value in medical pharmacology. This study involving these three selected plants was conducted to analyses the plasma testosterone concentration and testis histological features of mice supplemented with four different concentrations (50, 125, 500 and 1000 mg/kg b. wt) of AB, CC and PB ethanolic extract. There was a significant reduction in plasma testosterone level across all AB treatment groups (177.83±0.03pg/ml, 266.07±0.05pg/ml, 281.84±0.03pg/ml and 277.07±0.00 pg/ml, respectively) compared to control group (515.82±0.01pg/ml), in both 50 and 500mg/kg b. wt of CC (546.39±0.03pg/ml and 728.62±0.04, respectively), and in 50, 125 and 1000mg/kg b. wt of PB ethanolic extracts (375.84±0.05pg/ml, 865.96±0.03pg/ml and 473.15±0.01pg/ml, respectively). All concentrations of AB extract (206.77±5.51μm, 194.29±4.25μm, 202.92±3.34μm and 207.34±6.34μm, respectively) and 50 and 500 mg/kg b. wt of PB extract (212.30±5.57μm and 214.70±4.31μm, respectively) seem to reduce the diameter of seminiferous tubules, while comparing to the control (236.81±7.07μm). The lumen diameter in the seminiferous tubules also decreases in 125mg/kg b. wt of AB (70.14±3.09μm), 1000mg/kg b. wt of CC (73.47±2.80μm) and in the 50 and 125 mg/kg b. wt of PB extracts (72.65±3.99μm and 78.54±3.85μm, respectively). The spermatogenic cells in Figure1: C1, B2, C2, D2, E2, B3, C3 and E3 were packed and improved as compared to Figure 1A. The degeneration of spermatocyte and spermatogonia layer was also observed (Figure 1: B1, C1, D1, E1, D2, B3, C3, D3 and E3).

Science Letters, 2014

This article aims at reviewing the health benefits and potential effects of selected herbal plant... more This article aims at reviewing the health benefits and potential effects of selected herbal plants which included Averrhoa bilimbi, Cosmos caudatus and Pereskia bleo on mammalian reproductive systems. All these three herbal plants under study are enriching with potent anti-oxidant, anti-microbial and anti-carcinogen properties that are crucial in maintaining well-being. Averrhoa bilimbi has shown to increase infertility rate in adult female mice by using 14.4 ml/kg body weight of extracted Averrhoa bilimbi juice. Cosmos caudatus able to take action as therapeutic agent and repair bone damage in elderly women which phytoestrogens contains in it can mimic the effects of estrogen. Cosmos caudatus is reported to exhibit radical-scavenging activity which important to maintaining the production of quality sperm. Recognized for its anti-proliferation and cytotoxic activity that attacks a few human cancer cell lines, Pereskia bleo showed the ability to protect the normal mouse fibroblast cells from the natural or oxidant-induced cell death caused by programmed cell death or apoptosis.

Malaysian Applied Biology, 2015

This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus m... more This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus musculus. The daily intake of Cosmos caudatus was evaluated in determining its effects on male fertility. Four different concentrations of the crude ethanolic extracts which were 50 mg/kg, 125 mg/kg, 500 mg/kg and 1000 mg/kg, respectively were administered for 28 consecutive days. Sperm analyses includes sperm count, total sperm motility, sperm viability and sperm morphology were carried out using standard procedures outlined by WHO 2010. G3 group gives the highest mean percentage for change in body weight (9.60 gm), total sperm motility (56%) and normal sperm morphology count (62.8 sperm) as compared to the rest the group treatments. For the sperm count and sperm viability, G4 group showed the highest data; 56.5 million sperm/ml and 59.53%, respectively. The lowest data of the change in body weight and normal sperm morphology count of mice from G1 group which are 6.43 gm and 58.6 sperm, correspondingly. As for the sperm count, total sperm motility and sperm viability; the lowest data shown by G2 group; 39.4 million sperm/ml, 48% and 43.98%, respectively. The sperm morphology of all the treated male mice was normal except a few detected with abnormalities. The findings showed no negative effect of Cosmos caudatus on sperm quality of mice.

Article history: Received 23 July 2015 Accepted 25 August 2015 Available online 5 September 2015

This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus m... more This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus musculus. The daily intake of Cosmos caudatus was evaluated in determining its effects on male fertility. Four different concentrations of the crude ethanolic extracts which were 50 mg/kg, 125 mg/kg, 500 mg/kg and 1000 mg/kg, respectively were administered for 28 consecutive days. Sperm analyses includes sperm count, total sperm motility, sperm viability and sperm morphology were carried out using standard procedures outlined by WHO 2010. G3 group gives the highest mean percentage for change in body weight (9.60 gm), total sperm motility (56%) and normal sperm morphology count (62.8 sperm) as compared to the rest the group treatments. For the sperm count and sperm viability, G4 group showed the highest data; 56.5 million sperm/ml and 59.53%, respectively. The lowest data of the change in body weight and normal sperm morphology count of mice from G1 group which are 6.43 gm and 58.6 sperm, ...

Advances in Environmental Biology, 2015

Averrhoa bilimbi (AB), Cosmos caudatus (CC) and Pereskia bleo (PB) are among the local plants in ... more Averrhoa bilimbi (AB), Cosmos caudatus (CC) and Pereskia bleo (PB) are among the local plants in Malaysia enriched with plant secondary compounds scientifically proven to contain high value in medical pharmacology. This study involving these three selected plants was conducted to analyses the plasma testosterone concentration and testis histological features of mice supplemented with four different concentrations (50, 125, 500 and 1000 mg/kg b. wt) of AB, CC and PB ethanolic extract. There was a significant reduction in plasma testosterone level across all AB treatment groups (177.83±0.03pg/ml, 266.07±0.05pg/ml, 281.84±0.03pg/ml and 277.07±0.00 pg/ml, respectively) compared to control group (515.82±0.01pg/ml), in both 50 and 500mg/kg b. wt of CC (546.39±0.03pg/ml and 728.62±0.04, respectively), and in 50, 125 and 1000mg/kg b. wt of PB ethanolic extracts (375.84±0.05pg/ml, 865.96±0.03pg/ml and 473.15±0.01pg/ml, respectively). All concentrations of AB extract (206.77±5.51μm, 194.29±4.25μm, 202.92±3.34μm and 207.34±6.34μm, respectively) and 50 and 500 mg/kg b. wt of PB extract (212.30±5.57μm and 214.70±4.31μm, respectively) seem to reduce the diameter of seminiferous tubules, while comparing to the control (236.81±7.07μm). The lumen diameter in the seminiferous tubules also decreases in 125mg/kg b. wt of AB (70.14±3.09μm), 1000mg/kg b. wt of CC (73.47±2.80μm) and in the 50 and 125 mg/kg b. wt of PB extracts (72.65±3.99μm and 78.54±3.85μm, respectively). The spermatogenic cells in Figure1: C1, B2, C2, D2, E2, B3, C3 and E3 were packed and improved as compared to Figure 1A. The degeneration of spermatocyte and spermatogonia layer was also observed (Figure 1: B1, C1, D1, E1, D2, B3, C3, D3 and E3).

Science Letters, 2014

This article aims at reviewing the health benefits and potential effects of selected herbal plant... more This article aims at reviewing the health benefits and potential effects of selected herbal plants which included Averrhoa bilimbi, Cosmos caudatus and Pereskia bleo on mammalian reproductive systems. All these three herbal plants under study are enriching with potent anti-oxidant, anti-microbial and anti-carcinogen properties that are crucial in maintaining well-being. Averrhoa bilimbi has shown to increase infertility rate in adult female mice by using 14.4 ml/kg body weight of extracted Averrhoa bilimbi juice. Cosmos caudatus able to take action as therapeutic agent and repair bone damage in elderly women which phytoestrogens contains in it can mimic the effects of estrogen. Cosmos caudatus is reported to exhibit radical-scavenging activity which important to maintaining the production of quality sperm. Recognized for its anti-proliferation and cytotoxic activity that attacks a few human cancer cell lines, Pereskia bleo showed the ability to protect the normal mouse fibroblast cells from the natural or oxidant-induced cell death caused by programmed cell death or apoptosis.

Malaysian Applied Biology, 2015

This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus m... more This study was designed to analyse the effects of Cosmos caudatus on sperm quality of mice, Mus musculus. The daily intake of Cosmos caudatus was evaluated in determining its effects on male fertility. Four different concentrations of the crude ethanolic extracts which were 50 mg/kg, 125 mg/kg, 500 mg/kg and 1000 mg/kg, respectively were administered for 28 consecutive days. Sperm analyses includes sperm count, total sperm motility, sperm viability and sperm morphology were carried out using standard procedures outlined by WHO 2010. G3 group gives the highest mean percentage for change in body weight (9.60 gm), total sperm motility (56%) and normal sperm morphology count (62.8 sperm) as compared to the rest the group treatments. For the sperm count and sperm viability, G4 group showed the highest data; 56.5 million sperm/ml and 59.53%, respectively. The lowest data of the change in body weight and normal sperm morphology count of mice from G1 group which are 6.43 gm and 58.6 sperm, correspondingly. As for the sperm count, total sperm motility and sperm viability; the lowest data shown by G2 group; 39.4 million sperm/ml, 48% and 43.98%, respectively. The sperm morphology of all the treated male mice was normal except a few detected with abnormalities. The findings showed no negative effect of Cosmos caudatus on sperm quality of mice.