Gabriela Kania | Jagiellonian University in Krakow (original) (raw)
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Papers by Gabriela Kania
J. Mater. Chem. B, 2015
ABSTRACT Stable polymersomes with semipermeable membranes were prepared by simple mixing two oppo... more ABSTRACT Stable polymersomes with semipermeable membranes were prepared by simple mixing two oppositely charged diblock copolymers containing zwitterionic and cationic (PMPC20-b-PMAPTAC190) or anionic (PMPC20-b-PAMPS196) blocks. The formation of vesicular structures in the mixed solution of the block copolymers was confirmed by direct observation with cryo-TEM technique. Superparamagnetic iron oxide nanoparticles coated with cationic chitosan derivative (SPION/CCh) and these decorated with a fluorescent probe molecule were next incorporated into the polymersome structure. The average diameter of SPION/CCh-polymersomes estimated with cryo-TEM was about 250 nm. Surface topography of the SPION/CCh-loaded vesicles was imaged using AFM and magnetic properties of these objects were confirmed by MFM and MRI measurements. The ability of the SPION/CCh-polymersomes to affect T2 relaxation time in MRI was evaluated based on the measurements of r2 relaxivity. The obtained value of r2 (573 ± 10 mM-1s-1) was quite high. The cytotoxicity and intracellular uptake of the SPION/CCh-loaded vesicles into EA.hy926 cells were studied. The results indicate that the SPION/CCh-polymersomes seem to be internalized by vascular endothelium and are not cytotoxic to endothelial cells up to 1 μg Fe/ml. Therefore, it can be suggested that the SPION/CCh-polymersomes could prove useful as T2 contrast agents in MRI of endothelium.
Journal of Alloys and Compounds, 2014
We have synthesized the maghemite (g-Fe 2 O 3 ) nanoparticles by a chemical coprecipitation techn... more We have synthesized the maghemite (g-Fe 2 O 3 ) nanoparticles by a chemical coprecipitation technique of ferric and ferrous ions in alkali solution through the pipette drop method (pipette diameter: 2000 mm) and the piezoelectric nozzle method (nozzle size: 50 mm). The microstructures of nanoparticles were characterized by X-ray diffractometry (XRD) and transmission electron microscopy (TEM). The size distribution of the maghemite nanoparticles prepared by typical pipette drop method is from 5 to 8 nm. However, the nanoparticles made by piezoelectric nozzle method shows a small size and a very narrow size distribution from 3 to 5 nm. Zero-field-cooling (ZFC) and field-cooling (FC) magnetization measurements were performed using a superconducting quantum interference device magnetometer from 2 to 300 K to investigate the magnetic properties of nanoparticles. The FC/ZFC magnetization measurements showed a typical superparamagnetic behavior with very narrow size distribution. r
Journal of Nanoparticle Research, 2013
Journal of Nanoparticle Research, 2012
J. Mater. Chem. B, 2015
ABSTRACT Stable polymersomes with semipermeable membranes were prepared by simple mixing two oppo... more ABSTRACT Stable polymersomes with semipermeable membranes were prepared by simple mixing two oppositely charged diblock copolymers containing zwitterionic and cationic (PMPC20-b-PMAPTAC190) or anionic (PMPC20-b-PAMPS196) blocks. The formation of vesicular structures in the mixed solution of the block copolymers was confirmed by direct observation with cryo-TEM technique. Superparamagnetic iron oxide nanoparticles coated with cationic chitosan derivative (SPION/CCh) and these decorated with a fluorescent probe molecule were next incorporated into the polymersome structure. The average diameter of SPION/CCh-polymersomes estimated with cryo-TEM was about 250 nm. Surface topography of the SPION/CCh-loaded vesicles was imaged using AFM and magnetic properties of these objects were confirmed by MFM and MRI measurements. The ability of the SPION/CCh-polymersomes to affect T2 relaxation time in MRI was evaluated based on the measurements of r2 relaxivity. The obtained value of r2 (573 ± 10 mM-1s-1) was quite high. The cytotoxicity and intracellular uptake of the SPION/CCh-loaded vesicles into EA.hy926 cells were studied. The results indicate that the SPION/CCh-polymersomes seem to be internalized by vascular endothelium and are not cytotoxic to endothelial cells up to 1 μg Fe/ml. Therefore, it can be suggested that the SPION/CCh-polymersomes could prove useful as T2 contrast agents in MRI of endothelium.
Journal of Alloys and Compounds, 2014
We have synthesized the maghemite (g-Fe 2 O 3 ) nanoparticles by a chemical coprecipitation techn... more We have synthesized the maghemite (g-Fe 2 O 3 ) nanoparticles by a chemical coprecipitation technique of ferric and ferrous ions in alkali solution through the pipette drop method (pipette diameter: 2000 mm) and the piezoelectric nozzle method (nozzle size: 50 mm). The microstructures of nanoparticles were characterized by X-ray diffractometry (XRD) and transmission electron microscopy (TEM). The size distribution of the maghemite nanoparticles prepared by typical pipette drop method is from 5 to 8 nm. However, the nanoparticles made by piezoelectric nozzle method shows a small size and a very narrow size distribution from 3 to 5 nm. Zero-field-cooling (ZFC) and field-cooling (FC) magnetization measurements were performed using a superconducting quantum interference device magnetometer from 2 to 300 K to investigate the magnetic properties of nanoparticles. The FC/ZFC magnetization measurements showed a typical superparamagnetic behavior with very narrow size distribution. r
Journal of Nanoparticle Research, 2013
Journal of Nanoparticle Research, 2012