Hafifi Hashim - Profile on Academia.edu (original) (raw)
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The population structure of Hemibagrus nemurus in Tasik Chini Biosphere Reserve was investigated ... more The population structure of Hemibagrus nemurus in Tasik Chini Biosphere Reserve was investigated using D-loop DNA sequences. A total of 35 H. nemurus samples were collected from several locations in Tasik Chini and Sg. Pahang for this study. The neighbour-joining and maximum-parsimony analyses based on D-loop sequence data revealed that the H. nemurus samples were clustered into two major clades. The populations from Tasik Chini were grouped together with Sg. Pahang population with 98% bootstrap support. High gene flow (Nm: 2.16-23.3) was recorded among the populations and the pairwise subdivision (Fst) showed no significant value among the populations, indicating no genetic differentiation among the populations. The physical barrier viz. a dam in Sg. Chini does not seem to pose a threat to the genetic diversity of H. nemurus in the Tasik Chini Biosphere.
A phytotoxicity study was conducted to determine the maximum accumulation of Fe and Al in Scirpus... more A phytotoxicity study was conducted to determine the maximum accumulation of Fe and Al in Scirpus grossus and to isolate and identify the Fe-Al-resistant rhizobacteria from S. grossus roots. Plants were grown in artificial bauxite mining wastewater containing iron (Fe) and aluminum (Al) (at a mass ratio of 3:1) with five different Fe-Al mixture concentrations (ranging from 90 mg/L Fe + 30 mg/L Al to 450 mg/L Fe + 150 mg/L Al) together with a plant control (without the addition of Fe and Al) for 102 days. Maximum metal accumulation in plants was 53,187 mg Fe/kg and 8,864 mg Al/kg (dry basis), which occurred on day 42 in the 450 mg/L Fe + 150 mg/L Al treatment. Approximately 27 rhizobacteria were isolated from roots of all the treatments on day 102. Among these, three isolates, identified as Bacillus cereus strain NII, Bacillus pumilus strain NII, and Brevibacterium sp. strain NII, showed high resistance and tolerance to the highest Fe-Al concentrations (i.e., 450 mg/L Fe + 150 mg/L Al) in the medium. Thus, they can be considered Fe-Al-resistant rhizobacteria, since they survived and tolerated the most in Fe-and Al-rich medium during screening.
Fish community plays an important role in balancing the ecosystem in the lake. A study of species... more Fish community plays an important role in balancing the ecosystem in the lake. A study of species diversity of fish community was carried out in the lake of Tasik Chini Biosphere Reserve, Pekan, Pahang. Fish sampling at seven stations was conducted monthly from September 2013 to Februray 2014 using gill nets and fish traps. A total of 493 fishes consists 21 species were collected in this study. The most abundant fish species collected were Notopterus notopterus (20.28 %), followed by Labiobarbus festiva (16.43 %) and Osteochilus hasselti (16.22 %). The other species identified in this study were Pristolepsis fasciatus (10.75 %), Labiobarbus ocellatus (9.73%), Thynnichthys thynnoides (5.07%), Hampala macrolepidota (3.85%), Hemibagrus nemurus (3.24%). Most of the species found in this study were similarly reported in other studies in Tasik Chini. The Shannon index showing the fish diversity are 2.34, Pielou's Evennes index are 0.76 and Margalef's species Richness index are 3.38. The results of this study will be useful in contributing towards the management and conservation of Tasik Chini for sustainable manner for future generations.
The population structure of Hemibagrus nemurus in Tasik Chini Biosphere Reserve was investigated ... more The population structure of Hemibagrus nemurus in Tasik Chini Biosphere Reserve was investigated using D-loop DNA sequences. A total of 35 H. nemurus samples were collected from several locations in Tasik Chini and Sg. Pahang for this study. The neighbour-joining and maximum-parsimony analyses based on D-loop sequence data revealed that the H. nemurus samples were clustered into two major clades. The populations from Tasik Chini were grouped together with Sg. Pahang population with 98% bootstrap support. High gene flow (Nm: 2.16-23.3) was recorded among the populations and the pairwise subdivision (Fst) showed no significant value among the populations, indicating no genetic differentiation among the populations. The physical barrier viz. a dam in Sg. Chini does not seem to pose a threat to the genetic diversity of H. nemurus in the Tasik Chini Biosphere.
A phytotoxicity study was conducted to determine the maximum accumulation of Fe and Al in Scirpus... more A phytotoxicity study was conducted to determine the maximum accumulation of Fe and Al in Scirpus grossus and to isolate and identify the Fe-Al-resistant rhizobacteria from S. grossus roots. Plants were grown in artificial bauxite mining wastewater containing iron (Fe) and aluminum (Al) (at a mass ratio of 3:1) with five different Fe-Al mixture concentrations (ranging from 90 mg/L Fe + 30 mg/L Al to 450 mg/L Fe + 150 mg/L Al) together with a plant control (without the addition of Fe and Al) for 102 days. Maximum metal accumulation in plants was 53,187 mg Fe/kg and 8,864 mg Al/kg (dry basis), which occurred on day 42 in the 450 mg/L Fe + 150 mg/L Al treatment. Approximately 27 rhizobacteria were isolated from roots of all the treatments on day 102. Among these, three isolates, identified as Bacillus cereus strain NII, Bacillus pumilus strain NII, and Brevibacterium sp. strain NII, showed high resistance and tolerance to the highest Fe-Al concentrations (i.e., 450 mg/L Fe + 150 mg/L Al) in the medium. Thus, they can be considered Fe-Al-resistant rhizobacteria, since they survived and tolerated the most in Fe-and Al-rich medium during screening.
Fish community plays an important role in balancing the ecosystem in the lake. A study of species... more Fish community plays an important role in balancing the ecosystem in the lake. A study of species diversity of fish community was carried out in the lake of Tasik Chini Biosphere Reserve, Pekan, Pahang. Fish sampling at seven stations was conducted monthly from September 2013 to Februray 2014 using gill nets and fish traps. A total of 493 fishes consists 21 species were collected in this study. The most abundant fish species collected were Notopterus notopterus (20.28 %), followed by Labiobarbus festiva (16.43 %) and Osteochilus hasselti (16.22 %). The other species identified in this study were Pristolepsis fasciatus (10.75 %), Labiobarbus ocellatus (9.73%), Thynnichthys thynnoides (5.07%), Hampala macrolepidota (3.85%), Hemibagrus nemurus (3.24%). Most of the species found in this study were similarly reported in other studies in Tasik Chini. The Shannon index showing the fish diversity are 2.34, Pielou's Evennes index are 0.76 and Margalef's species Richness index are 3.38. The results of this study will be useful in contributing towards the management and conservation of Tasik Chini for sustainable manner for future generations.