Aneta Ptaszyńska | Maria Curie - Skłodowska University (original) (raw)
Papers by Aneta Ptaszyńska
Fems Microbiology Letters, Jul 17, 2014
Nosemosis is a contagious disease of honeybees (Apis mellifera) manifested by increased winter mo... more Nosemosis is a contagious disease of honeybees (Apis mellifera) manifested by increased winter mortality, poor spring build-up and even the total extinction of infected bee colonies. In this paper, loop-mediated isothermal amplifications (LAMP) were used for the first time to identify and differentiate N. apis and N. ceranae, the causative agents of nosemosis. LAMP assays were performed at a constant temperature of 60°C using two sets of six species-specific primers, recognising eight distinct fragments of 16S rDNA gene and GspSSD polymerase with strand displacement activity. The optimal time for LAMP and its Nosema species sensitivity and specificity were assessed. LAMP only required 30 min for robust identification of the amplicons. Tenfold serial dilutions of total DNA isolated from bees infected with microsporidia were used to determine the detection limit of N. apis and N. ceranae DNAs by LAMP and standard PCR assays. LAMP appeared to be 10 3-fold more sensitive than a standard PCR in detecting N. apis and N. ceranae. LAMP methods developed by us are highly Nosema species specific and allow to identify and differentiate N. apis and N. ceranae.
Wiadomości Entomologiczne, 2005
Applied sciences, Apr 14, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Aneta Ptaszynska 2018. Changes in bioelement contents of summer and winter western honeybees (Api... more Aneta Ptaszynska 2018. Changes in bioelement contents of summer and winter western honeybees (Apis mellifera) induced by Nosema ceranae infection. protocols.io protocols.io
Molecules, Dec 3, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Medycyna Weterynaryjna, 2013
GA – 70th Annual Meeting 2022
Molecules
Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. is well-known for its adaptogenic properties ... more Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. is well-known for its adaptogenic properties in traditional Eastern medicine. It has been categorized as an endangered species due to the over-exploitation of the roots. As a result, alternatives must be found, including the usage of renewable aerial parts such as fruits. The goal of this research was to determine the phenolic compounds and the enzymatic, antioxidant, and cytotoxic activities of the intractum gained from the E. senticosus fruits and the mixture of chloroform-methanol roots extract with naringenin (3:7:5). The obtained results showed, that the intractum contained 1.02 mg/g ext. of polyphenols, 0.30 mg/g ext. of flavonoids, and 0.19 mg/g ext. of phenolic acids. In turn, the mixture of chloroform-methanol roots extract with naringenin (3:7:5) contained 159.27 mg/g ext. of polyphenols, 137.47 mg/g ext. of flavonoids, and 79.99 mg/g ext. of phenolic acids. Regarding the anti-enzymatic assay, the IC50 values for tyrosina...
Planta Medica, Dec 1, 2022
International Journal of Environmental Research and Public Health
Due to a widespread occurrence of multidrug-resistant pathogenic strains of bacteria, there is an... more Due to a widespread occurrence of multidrug-resistant pathogenic strains of bacteria, there is an urgent need to look for antimicrobial substances, and honey with its antimicrobial properties is a very promising substance. In this study, we examined for the first time antimicrobial properties of novel varietal honeys, i.e., plum, rapeseed, Lime, Phacelia, honeydew, sunflower, willow, and multifloral-P (Prunus spinosa L.), multifloral-AP (Acer negundo L., Prunus spinosa L.), multifloral-Sa (Salix sp.), multifloral-Br (Brassica napus L.). Their antimicrobial activity was tested against bacteria (such as Escherichia coli, Bacillus circulans, Staphylococcus aureus, Pseudomonas aeruginosa), yeasts (such as Saccharomyces cerevisiae and Candida albicans) and mold fungi (such as Aspergillus niger). In tested honeys, phenolic acids constituted one of the most important groups of compounds with antimicrobial properties. Our study found phenolic acids to occur in greatest amount in honeydew ho...
Micron, 2014
The extract from Pelargonium zonale stalks exhibits activity against Candida albicans and exerts ... more The extract from Pelargonium zonale stalks exhibits activity against Candida albicans and exerts an effect on the HeLa cell line. The action against C. albicans cells was analysed using light, CLSM, SEM, and TEM microscopes. The observations indicate that the extract influenced fungal cell morphology and cell metabolic activity. The morphological changes include cell wall damage, deformations of cell surfaces, and abnormalities in fungal cell shape and size. Cells of C. albicans treated with the extract exhibited disturbances in the budding pattern and a tendency to form agglomerates and multicellular chains. The P. zonale extract caused a significant decrease in the metabolic activity of C. albicans cells. Cells died via both apoptosis and necrosis. The antitumor activity of the extract was analysed using the MTT assay. The P. zonale extract exhibited minor cytotoxicity against the HeLa cell line but a dose-dependent cytopathic effect was noticed. The P. zonale extract is a promising source for the isolation of antifungal and anticancer compounds.
Pathogens
Honeybees are important pollinators, but they are continuously exposed to a variety of fungal and... more Honeybees are important pollinators, but they are continuously exposed to a variety of fungal and bacterial diseases. One of the various diseases affecting honeybees is nosemosis caused by microsporidia from the Nosema genus. Honeybees are mainly infected through consumption of infected food or faeces containing Nosema spp. spores. Nosemosis causes damage to the middle intestine epithelium, which leads to food absorption disorders and honeybee malnutrition. Fumagillin, i.e., the antibiotic used to treat nosemosis, was withdrawn in 2016 from EU countries. Therefore, researchers have been looking for compounds of both natural and synthetic origin to fight nosemosis. Such compounds should not have a negative impact on bees but is expected to inhibit the disease. Natural compounds tested against nosemosis include, e.g., essential oils (EOs), plant extracts, propolis, and bacterial metabolites, while synthetic substances tested as anti-nosemosis agents are represented by porphyrins, vita...
Journal of Raman Spectroscopy, 2015
Dietary fibres are regarded as the source of polysaccharides and antioxidants such as polyphenols... more Dietary fibres are regarded as the source of polysaccharides and antioxidants such as polyphenols. However, addition of dietary fibre to bread causes significant reduction in its quality. The bread quality is connected with the structure of gluten proteins. For this reason, Fourier transform Raman spectroscopy was applied to determine changes in structure of gluten proteins modified by seven dietary fibres. The fibres were added to model flour reconstituted with wheat gluten and wheat starch. The model flour was used to provide gluten proteins of definite structure. The obtained results showed that six out of seven fibres caused similar changes in β-turn structures. The appearance of the band at 1642 cm À1 and the shift toward lower wavenumbers of the band at 1670 cm À1 in the difference spectra indicated hydrogen bonding of carbonyl groups in β-turns leading to protein folding/aggregation. Addition of fibre preparations caused also changes in conformation of disulfide bridges (S-S), corresponding to transformation to trans-gauche-gauche and trans-gauche-trans conformations at the expense of the stable gauche-gauchegauche conformation. The S-S bonds in less stable conformations were formed inside the protein complex as well as between protein complexes in the form of β-structures. Generally, the observed changes in gluten proteins after addition of dietary fibres were results of interactions between fibre polysaccharides and gluten proteins rather than between polyphenols and gluten proteins.
Nowadays, there is an urgent need to look for antimicrobial substances due to a widespread occurr... more Nowadays, there is an urgent need to look for antimicrobial substances due to a widespread occurrence of multidrug resistant bacterial and fungal strains. Honey with its antimicrobial properties is a very promising substance with many valuable properties. In this study we examined for the first time antimicrobial properties of novel varietal honeys. The experiments were carried out with 11 honey samples, i.e.: Plum, Rapeseed, Lime, Phacelia, Honeydew, Sunflower, Willow, and Multifloral-P (Prunus spinosa L.), Multifloral-AP (Acer negundo L., Prunus spinosa L.), Multifloral-Sa (Salix sp.), Multifloral-Br (Brassica napus L.). Their antimicrobial activity was tested against bacteria (such as Escherichia coli, Bacillus circulans, Staphylococcus aureus, Pseudomonas aeruginosa), yeasts (such as Saccharomyces cerevisiae and Candida albicans) and mold fungi (such as Aspergillus niger). In tested honeys, phenolic acids were one of the most important group of compounds with antimicrobial activ...
Journal of Experimental Biology and Agricultural Sciences, 2021
Buckwheat honey is widely consumed by consumers due to its numerous health-promoting properties. ... more Buckwheat honey is widely consumed by consumers due to its numerous health-promoting properties. Characteristically it is dark, tea-like in colour, sharp, tickly, and sweet in flavour, and has smelled of buckwheat flowers. In the current study, various commercial honey samples were examined to test the quality of buckwheat honey samples available in the market. The research materials were comprised of 15 samples of honeys from 4 voivodships, among these, 5 samples were collected from the Świętokrzyskie Voivodship, 4 from the Lesser Poland Voivodeship, 4 from the Lubelskie Voivodship, and reset 2 samples from the Podkarpackie Voivodship. Melissopalynological analyses of investigated honeys’ samples revealed that all samples had at least 45% of Fagopyrym pollen content, which means that researched honeys complied with the standards of the International Commission for Bee Botany (ICBB) for buckwheat honey. Honeys’ samples had an average water content of 15.3% (σ= 1.24), and electrical ...
Journal of Fungi, 2021
Fall armyworm, Spodoptera frugiperda, entered Thailand in late 2018 and has now spread in several... more Fall armyworm, Spodoptera frugiperda, entered Thailand in late 2018 and has now spread in several regions, with devastating effects in maize and rice production, which are some of the most important cereals in the world. Since then, farmers have utilized the available chemical insecticides to try to control it, but their efforts have been futile. Instead, they have ended up using extraordinary dosages, hence threatening non-target species and other fauna and flora, as well as being costly. In this regard, research has been ongoing, aiming to come up with eco-friendly solutions for this insect. We surveyed and collected various isolates of native entomopathogenic fungi intending to test their efficacy against fall armyworm. Six isolates of entomopathogenic fungi were obtained and identified to Beauveria bassiana based on morphological characteristics and multi-gene phylogenetic analyses. Thereafter, the six isolates of B. bassiana were used to perform efficacy experiments against fal...
Biochemistry (Moscow), 2019
The lifespan of an organism depends on ATP pro duction, among other factors [1]. The initial subs... more The lifespan of an organism depends on ATP pro duction, among other factors [1]. The initial substrate in ATP synthesis is glucose. The levels and activities of glu coneogenesis and glycolysis enzymes are controlled so that both pathways are not activated at the same time. The rate of glycolysis is regulated by the concentration of glu cose, whereas the rate of gluconeogenesis-by the con centrations of lactate and other glucose precursors [2]. In
Scientific reports, Jan 3, 2018
The study of organic/inorganic molecules with activity against intracellular fungi of the phylum ... more The study of organic/inorganic molecules with activity against intracellular fungi of the phylum Microsporidia is of critical importance. Here, for the first time, the inactivation of these parasitic fungi by porphyrins is reported. The biological effects of porphyrins (10 µM and 100 µM) on the microsporidian Nosema ceranae was investigated in honeybee hosts using cage experiments. A significant reduction in the number of spores (from 2.6 to 5 fold) was observed in Nosema-infected honeybees with a sucrose-protoporphyrin amide [PP(Asp)] syrup diet compared to the control honeybees. PP(Asp) and the other porphyrin examined in vitro, TMePyP, had a direct impact on the microsporidia. Notably, neither porphyrin requires light excitation to be active against microsporidia. Moreover, microsporidia preincubated with these porphyrins exhibited decreased ability to infect honeybees. In particular, PP(Asp), possessing amphiphilic characteristics, exhibited significant inactivation of microspor...
Fems Microbiology Letters, Jul 17, 2014
Nosemosis is a contagious disease of honeybees (Apis mellifera) manifested by increased winter mo... more Nosemosis is a contagious disease of honeybees (Apis mellifera) manifested by increased winter mortality, poor spring build-up and even the total extinction of infected bee colonies. In this paper, loop-mediated isothermal amplifications (LAMP) were used for the first time to identify and differentiate N. apis and N. ceranae, the causative agents of nosemosis. LAMP assays were performed at a constant temperature of 60°C using two sets of six species-specific primers, recognising eight distinct fragments of 16S rDNA gene and GspSSD polymerase with strand displacement activity. The optimal time for LAMP and its Nosema species sensitivity and specificity were assessed. LAMP only required 30 min for robust identification of the amplicons. Tenfold serial dilutions of total DNA isolated from bees infected with microsporidia were used to determine the detection limit of N. apis and N. ceranae DNAs by LAMP and standard PCR assays. LAMP appeared to be 10 3-fold more sensitive than a standard PCR in detecting N. apis and N. ceranae. LAMP methods developed by us are highly Nosema species specific and allow to identify and differentiate N. apis and N. ceranae.
Wiadomości Entomologiczne, 2005
Applied sciences, Apr 14, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Aneta Ptaszynska 2018. Changes in bioelement contents of summer and winter western honeybees (Api... more Aneta Ptaszynska 2018. Changes in bioelement contents of summer and winter western honeybees (Apis mellifera) induced by Nosema ceranae infection. protocols.io protocols.io
Molecules, Dec 3, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Medycyna Weterynaryjna, 2013
GA – 70th Annual Meeting 2022
Molecules
Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. is well-known for its adaptogenic properties ... more Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. is well-known for its adaptogenic properties in traditional Eastern medicine. It has been categorized as an endangered species due to the over-exploitation of the roots. As a result, alternatives must be found, including the usage of renewable aerial parts such as fruits. The goal of this research was to determine the phenolic compounds and the enzymatic, antioxidant, and cytotoxic activities of the intractum gained from the E. senticosus fruits and the mixture of chloroform-methanol roots extract with naringenin (3:7:5). The obtained results showed, that the intractum contained 1.02 mg/g ext. of polyphenols, 0.30 mg/g ext. of flavonoids, and 0.19 mg/g ext. of phenolic acids. In turn, the mixture of chloroform-methanol roots extract with naringenin (3:7:5) contained 159.27 mg/g ext. of polyphenols, 137.47 mg/g ext. of flavonoids, and 79.99 mg/g ext. of phenolic acids. Regarding the anti-enzymatic assay, the IC50 values for tyrosina...
Planta Medica, Dec 1, 2022
International Journal of Environmental Research and Public Health
Due to a widespread occurrence of multidrug-resistant pathogenic strains of bacteria, there is an... more Due to a widespread occurrence of multidrug-resistant pathogenic strains of bacteria, there is an urgent need to look for antimicrobial substances, and honey with its antimicrobial properties is a very promising substance. In this study, we examined for the first time antimicrobial properties of novel varietal honeys, i.e., plum, rapeseed, Lime, Phacelia, honeydew, sunflower, willow, and multifloral-P (Prunus spinosa L.), multifloral-AP (Acer negundo L., Prunus spinosa L.), multifloral-Sa (Salix sp.), multifloral-Br (Brassica napus L.). Their antimicrobial activity was tested against bacteria (such as Escherichia coli, Bacillus circulans, Staphylococcus aureus, Pseudomonas aeruginosa), yeasts (such as Saccharomyces cerevisiae and Candida albicans) and mold fungi (such as Aspergillus niger). In tested honeys, phenolic acids constituted one of the most important groups of compounds with antimicrobial properties. Our study found phenolic acids to occur in greatest amount in honeydew ho...
Micron, 2014
The extract from Pelargonium zonale stalks exhibits activity against Candida albicans and exerts ... more The extract from Pelargonium zonale stalks exhibits activity against Candida albicans and exerts an effect on the HeLa cell line. The action against C. albicans cells was analysed using light, CLSM, SEM, and TEM microscopes. The observations indicate that the extract influenced fungal cell morphology and cell metabolic activity. The morphological changes include cell wall damage, deformations of cell surfaces, and abnormalities in fungal cell shape and size. Cells of C. albicans treated with the extract exhibited disturbances in the budding pattern and a tendency to form agglomerates and multicellular chains. The P. zonale extract caused a significant decrease in the metabolic activity of C. albicans cells. Cells died via both apoptosis and necrosis. The antitumor activity of the extract was analysed using the MTT assay. The P. zonale extract exhibited minor cytotoxicity against the HeLa cell line but a dose-dependent cytopathic effect was noticed. The P. zonale extract is a promising source for the isolation of antifungal and anticancer compounds.
Pathogens
Honeybees are important pollinators, but they are continuously exposed to a variety of fungal and... more Honeybees are important pollinators, but they are continuously exposed to a variety of fungal and bacterial diseases. One of the various diseases affecting honeybees is nosemosis caused by microsporidia from the Nosema genus. Honeybees are mainly infected through consumption of infected food or faeces containing Nosema spp. spores. Nosemosis causes damage to the middle intestine epithelium, which leads to food absorption disorders and honeybee malnutrition. Fumagillin, i.e., the antibiotic used to treat nosemosis, was withdrawn in 2016 from EU countries. Therefore, researchers have been looking for compounds of both natural and synthetic origin to fight nosemosis. Such compounds should not have a negative impact on bees but is expected to inhibit the disease. Natural compounds tested against nosemosis include, e.g., essential oils (EOs), plant extracts, propolis, and bacterial metabolites, while synthetic substances tested as anti-nosemosis agents are represented by porphyrins, vita...
Journal of Raman Spectroscopy, 2015
Dietary fibres are regarded as the source of polysaccharides and antioxidants such as polyphenols... more Dietary fibres are regarded as the source of polysaccharides and antioxidants such as polyphenols. However, addition of dietary fibre to bread causes significant reduction in its quality. The bread quality is connected with the structure of gluten proteins. For this reason, Fourier transform Raman spectroscopy was applied to determine changes in structure of gluten proteins modified by seven dietary fibres. The fibres were added to model flour reconstituted with wheat gluten and wheat starch. The model flour was used to provide gluten proteins of definite structure. The obtained results showed that six out of seven fibres caused similar changes in β-turn structures. The appearance of the band at 1642 cm À1 and the shift toward lower wavenumbers of the band at 1670 cm À1 in the difference spectra indicated hydrogen bonding of carbonyl groups in β-turns leading to protein folding/aggregation. Addition of fibre preparations caused also changes in conformation of disulfide bridges (S-S), corresponding to transformation to trans-gauche-gauche and trans-gauche-trans conformations at the expense of the stable gauche-gauchegauche conformation. The S-S bonds in less stable conformations were formed inside the protein complex as well as between protein complexes in the form of β-structures. Generally, the observed changes in gluten proteins after addition of dietary fibres were results of interactions between fibre polysaccharides and gluten proteins rather than between polyphenols and gluten proteins.
Nowadays, there is an urgent need to look for antimicrobial substances due to a widespread occurr... more Nowadays, there is an urgent need to look for antimicrobial substances due to a widespread occurrence of multidrug resistant bacterial and fungal strains. Honey with its antimicrobial properties is a very promising substance with many valuable properties. In this study we examined for the first time antimicrobial properties of novel varietal honeys. The experiments were carried out with 11 honey samples, i.e.: Plum, Rapeseed, Lime, Phacelia, Honeydew, Sunflower, Willow, and Multifloral-P (Prunus spinosa L.), Multifloral-AP (Acer negundo L., Prunus spinosa L.), Multifloral-Sa (Salix sp.), Multifloral-Br (Brassica napus L.). Their antimicrobial activity was tested against bacteria (such as Escherichia coli, Bacillus circulans, Staphylococcus aureus, Pseudomonas aeruginosa), yeasts (such as Saccharomyces cerevisiae and Candida albicans) and mold fungi (such as Aspergillus niger). In tested honeys, phenolic acids were one of the most important group of compounds with antimicrobial activ...
Journal of Experimental Biology and Agricultural Sciences, 2021
Buckwheat honey is widely consumed by consumers due to its numerous health-promoting properties. ... more Buckwheat honey is widely consumed by consumers due to its numerous health-promoting properties. Characteristically it is dark, tea-like in colour, sharp, tickly, and sweet in flavour, and has smelled of buckwheat flowers. In the current study, various commercial honey samples were examined to test the quality of buckwheat honey samples available in the market. The research materials were comprised of 15 samples of honeys from 4 voivodships, among these, 5 samples were collected from the Świętokrzyskie Voivodship, 4 from the Lesser Poland Voivodeship, 4 from the Lubelskie Voivodship, and reset 2 samples from the Podkarpackie Voivodship. Melissopalynological analyses of investigated honeys’ samples revealed that all samples had at least 45% of Fagopyrym pollen content, which means that researched honeys complied with the standards of the International Commission for Bee Botany (ICBB) for buckwheat honey. Honeys’ samples had an average water content of 15.3% (σ= 1.24), and electrical ...
Journal of Fungi, 2021
Fall armyworm, Spodoptera frugiperda, entered Thailand in late 2018 and has now spread in several... more Fall armyworm, Spodoptera frugiperda, entered Thailand in late 2018 and has now spread in several regions, with devastating effects in maize and rice production, which are some of the most important cereals in the world. Since then, farmers have utilized the available chemical insecticides to try to control it, but their efforts have been futile. Instead, they have ended up using extraordinary dosages, hence threatening non-target species and other fauna and flora, as well as being costly. In this regard, research has been ongoing, aiming to come up with eco-friendly solutions for this insect. We surveyed and collected various isolates of native entomopathogenic fungi intending to test their efficacy against fall armyworm. Six isolates of entomopathogenic fungi were obtained and identified to Beauveria bassiana based on morphological characteristics and multi-gene phylogenetic analyses. Thereafter, the six isolates of B. bassiana were used to perform efficacy experiments against fal...
Biochemistry (Moscow), 2019
The lifespan of an organism depends on ATP pro duction, among other factors [1]. The initial subs... more The lifespan of an organism depends on ATP pro duction, among other factors [1]. The initial substrate in ATP synthesis is glucose. The levels and activities of glu coneogenesis and glycolysis enzymes are controlled so that both pathways are not activated at the same time. The rate of glycolysis is regulated by the concentration of glu cose, whereas the rate of gluconeogenesis-by the con centrations of lactate and other glucose precursors [2]. In
Scientific reports, Jan 3, 2018
The study of organic/inorganic molecules with activity against intracellular fungi of the phylum ... more The study of organic/inorganic molecules with activity against intracellular fungi of the phylum Microsporidia is of critical importance. Here, for the first time, the inactivation of these parasitic fungi by porphyrins is reported. The biological effects of porphyrins (10 µM and 100 µM) on the microsporidian Nosema ceranae was investigated in honeybee hosts using cage experiments. A significant reduction in the number of spores (from 2.6 to 5 fold) was observed in Nosema-infected honeybees with a sucrose-protoporphyrin amide [PP(Asp)] syrup diet compared to the control honeybees. PP(Asp) and the other porphyrin examined in vitro, TMePyP, had a direct impact on the microsporidia. Notably, neither porphyrin requires light excitation to be active against microsporidia. Moreover, microsporidia preincubated with these porphyrins exhibited decreased ability to infect honeybees. In particular, PP(Asp), possessing amphiphilic characteristics, exhibited significant inactivation of microspor...