Mihail Buse - Academia.edu (original) (raw)

Papers by Mihail Buse

Additional file 1: Figure S1. The experimental workflow for the multiple dose exposure, at each 4... more Additional file 1: Figure S1. The experimental workflow for the multiple dose exposure, at each 4 days cells were passaged and added fresh medium with 50 nm Dox.

Additional file 9: Table S9. NGS sequencing data for bladder cancer.

Additional file 8: Figure S3. A Network analysis of the mutated genes in relation to their target... more Additional file 8: Figure S3. A Network analysis of the mutated genes in relation to their targeted miRNAs in bladder cancer. Schematic representation was obtained from miRnet ( https://www.mirnet.ca/ ).

Additional file 6: Figure S1. Bladder cancer survival curves showing the overall survival rate fo... more Additional file 6: Figure S1. Bladder cancer survival curves showing the overall survival rate for TCGA bladder cancer patients for the most frequent mutated genes retrieved in our study, bases on mutation status.

Additional file 5: Table S5. miRNA with and altered expression level in high grade versus low gra... more Additional file 5: Table S5. miRNA with and altered expression level in high grade versus low grade bladder cancer- TCGA patient cohort.

Additional file 4: Table S4. miRNA with and altered expression level in high grade versus low gra... more Additional file 4: Table S4. miRNA with and altered expression level in high grade versus low grade bladder cancer- UMPh patient cohort.

Additional file 3: Table S3. The main altered miRNAs 409 tumours and 19 tumour-adjacent normal ti... more Additional file 3: Table S3. The main altered miRNAs 409 tumours and 19 tumour-adjacent normal tissues retrieved from TCGA (fold changeÂą2, p-valued â ¤0.05).

Additional file 2: Table S2. Demographic and histopathological characteristics of TCGA bladder ca... more Additional file 2: Table S2. Demographic and histopathological characteristics of TCGA bladder cancer patient cohort.

Additional file 1: Table S1. The main altered miRNAs in the case of 23 matched paired samples (fo... more Additional file 1: Table S1. The main altered miRNAs in the case of 23 matched paired samples (fold changeÂą2, p-valued â ¤0.05).

Additional file 7: Figure S2. Target gene network generated using String10.5 based on the interac... more Additional file 7: Figure S2. Target gene network generated using String10.5 based on the interactions from the miRTarBase database for (A) miR-23a-3p (B) miR-139-5p, (C) miR-141-3p, (D) miR-143-5p and (F) miR-205-5p.

Journal of Cellular and Molecular Medicine, 2020

This is an open access article under the terms of the Creative Commons Attribution License, which... more This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 2018

Correspondence to: Mihail Buse, PhD. MEDFUTURE Research Center for Advanced Medicine, “Iuliu-Hati... more Correspondence to: Mihail Buse, PhD. MEDFUTURE Research Center for Advanced Medicine, “Iuliu-Hatieganu” University of Medicine and Pharmacy, Gheorghe Marinescu 23/Loius Pasteur 4-6 street (Complex), 400337, Cluj-Napoca, Romania. Tel: +40 752080169, E-mail: da.buse@gmail.com Received: 17/01/2018; Accepted: 25/02/2018 Literature review on the feasibility of laparoscopic partial nephrectomy for renal cancer during pregnancy: a propos of a case

Ovarian cancer is one of the most common cancers worldwide, and is associated with a prior diagno... more Ovarian cancer is one of the most common cancers worldwide, and is associated with a prior diagnosis of endometriosis in several cases. Our aim was to correlate genetic and methylation profile of ovarian endometrioid ovarian cancer and endometriosis patients. We evaluated the genetic profile of 50 ovarian endometriosis and 20 ovarian endometrioid carcinoma samples using next generation sequencing technology. In addition, the DNA methylation profile was evaluated for both cohorts of patients. We observed several mutated genes that were common for both types of patients, but we also identified mutated genes that were characteristic for each group: JAK3, KRAS and RB1 for endometriosis; and ATM, BRAF, CDH1, EGFR, NRAS, RET and SMO for ovarian endometrioid cancer. Also we idenfied genes that are highly methylated only in endometriosis samples (PYCARD, RARB, RB1, IL2, CFTR, CD44 and CDH13) and MLH3 gene was methylated only in endometrioid ovarian carcinoma samples. Also, BRCA1, CADM1, PAX...

Journal of Experimental & Clinical Cancer Research, 2019

Background Bladder cancer (BC) is a common urothelial malignancy, characterized by a high recurre... more Background Bladder cancer (BC) is a common urothelial malignancy, characterized by a high recurrence rate. The biology of bladder cancer is complex and needs to be deciphered. The latest evidence reveals the critical role of the non-coding RNAs, particularly microRNAs (miRNAs), as vital regulatory elements in cancer. Method We performed a miRNAs microarray using paired tissues (tumor and adjacent normal bladder tissue), followed by the validation with qRT-PCR of five selected transcripts. Additional next-generation sequencing investigation established the interconnection among the altered miRNAs and mutated genes. Based on the overlapping between TCGA data and data obtained in the study, we focused on the systematic identification of altered miRNAs and genes mutated involved in bladder cancer tumorigenesis and progression. Results By overlapping the miRNAs expression data, the two patient cohorts, we identified 18 miRNAs downregulated and, 187 miRNAs upregulated. qRT-PCR validation ...

Cancers, 2019

The mitogen-activated protein kinase (MAPK) pathway is an important bridge in the switch from ext... more The mitogen-activated protein kinase (MAPK) pathway is an important bridge in the switch from extracellular signals to intracellular responses. Alterations of signaling cascades are found in various diseases, including cancer, as a result of genetic and epigenetic changes. Numerous studies focused on both the homeostatic and the pathologic conduct of MAPK signaling; however, there is still much to be deciphered in terms of regulation and action models in both preclinical and clinical research. MAPK has implications in the response to cancer therapy, particularly the activation of the compensatory pathways in response to experimental MAPK inhibition. The present paper discusses new insights into MAPK as a complex cell signaling pathway with roles in the sustenance of cellular normal conduit, response to cancer therapy, and activation of compensatory pathways. Unfortunately, most MAPK inhibitors trigger resistance due to the activation of compensatory feed-back loops in tumor cells an...

Medicina, 2019

Background and objectives: Papillary thyroid carcinoma is the most frequent variety of all malign... more Background and objectives: Papillary thyroid carcinoma is the most frequent variety of all malignant endocrine tumors. It represents a heterogeneous malignancy with various clinical outcomes, emphasizing the need to identify powerful biomarkers with clinical relevance. Materials and Methods: Available gene expression data (level 3) for thyroid cancers were downloaded from the Cancer Genome Atlas (TCGA), followed by bioinformatic analyses performed on the data set. Results: Based on gene expression analysis, we were able to identify common and specific gene signatures for the three main types of papillary thyroid carcinoma (classical, follicular variant, and tall-cell). The survival rate was not significantly different among the main subtypes, but we were able to identify a biological adhesion signature with impact in patient prognostic. Conclusions: Taken together, the gene expression signature and particular adhesion signature, along with ITGA10 and MSLN in particular, could be use...

Journal of Clinical Medicine, 2019

During recent decades, understanding of the molecular mechanisms of acute lymphoblastic leukemia ... more During recent decades, understanding of the molecular mechanisms of acute lymphoblastic leukemia (ALL) has improved considerably, resulting in better risk stratification of patients and increased survival rates. Age, white blood cell count (WBC), and specific genetic abnormalities are the most important factors that define risk groups for ALL. State-of-the-art diagnosis of ALL requires cytological and cytogenetical analyses, as well as flow cytometry and high-throughput sequencing assays. An important aspect in the diagnostic characterization of patients with ALL is the identification of the Philadelphia (Ph) chromosome, which warrants the addition of tyrosine kinase inhibitors (TKI) to the chemotherapy backbone. Data that support the benefit of hematopoietic stem cell transplantation (HSCT) in high risk patient subsets or in late relapse patients are still questioned and have yet to be determined conclusive. This article presents the newly published data in ALL workup and treatment...

International Journal of Molecular Sciences, 2019

Mass spectrometry (MS) is an essential analytical technology on which the emerging omics domains;... more Mass spectrometry (MS) is an essential analytical technology on which the emerging omics domains; such as genomics; transcriptomics; proteomics and metabolomics; are based. This quantifiable technique allows for the identification of thousands of proteins from cell culture; bodily fluids or tissue using either global or targeted strategies; or detection of biologically active metabolites in ultra amounts. The routine performance of MS technology in the oncological field provides a better understanding of human diseases in terms of pathophysiology; prevention; diagnosis and treatment; as well as development of new biomarkers; drugs targets and therapies. In this review; we argue that the recent; successful advances in MS technologies towards cancer omics studies provides a strong rationale for its implementation in biomedicine as a whole.

Nanomedicine, 2019

Gold nanoparticles have drawn attention to nanomedicine for many years due to their physicochemic... more Gold nanoparticles have drawn attention to nanomedicine for many years due to their physicochemical properties, which include: good stability; biocompatibility; easy surface chemistry and superior magnetic; and last, electronic properties. All of these properties distinguish gold nanoparticles as advantageous carriers to be exploited. The challenge to develop new gold nanostructures has led to anisotropy, a new property to exploit for various medical applications: diagnostic and imaging strategies as well as therapeutic options. Gold nanorods are the most studied anisotropic gold nanoparticles because of the presence of two absorption peaks according to their longitudinal and transversal plasmon resonances. The longitudinal surface plasmonic resonance can provide the absorption in the near-infrared region and this is an important aspect of using gold nanorods for medical purposes.

Current genomics, 2018

Lung cancer continues to be the leading topic concerning global mortality rate caused by can-cer;... more Lung cancer continues to be the leading topic concerning global mortality rate caused by can-cer; it needs to be further investigated to reduce these dramatic unfavorable statistic data. Non-coding RNAs (ncRNAs) have been shown to be important cellular regulatory factors and the alteration of their expression levels has become correlated to extensive number of pathologies. Specifically, their expres-sion profiles are correlated with development and progression of lung cancer, generating great interest for further investigation. This review focuses on the complex role of non-coding RNAs, namely miR-NAs, piwi-interacting RNAs, small nucleolar RNAs, long non-coding RNAs and circular RNAs in the process of developing novel biomarkers for diagnostic and prognostic factors that can then be utilized for personalized therapies toward this devastating disease. To support the concept of personalized medi-cine, we will focus on the roles of miRNAs in lung cancer tumorigenesis, their use as dia...