Benjamin Bythell | University of Missouri - St. Louis (original) (raw)
Papers by Benjamin Bythell
The journal of physical chemistry. A, Jan 13, 2014
Recently, I explored structurally straightforward pathways to Cα hydrogen atom, H(•), transfer re... more Recently, I explored structurally straightforward pathways to Cα hydrogen atom, H(•), transfer reactions in the radical cation complex following electron capture/transfer of a series of polyprotonated peptides (J. Phys. Chem. A 2013, 117, 1189-1196). Here, I extend my analysis to incorporate detailed rearrangement processes potentially occurring prior to H(•) transfer. This comprises intracomplex isomerization of the initial iminol-terminated (-C(OH)═NH) form of the cn' species to the energetically more favorable, amide-terminated form (-C(O)-NH2) prior to Cα H(•) abstraction by the zm(•) species. The data indicate that the previously published H(•) transfer barriers are more energetically demanding than those of this multistep alternative. The rate-determining step is typically the intracomplex iminol isomerization, consistent with the substantial energetic favorability of the amide form of the cn species. The barriers to H(•) transfer still rise steeply as a function of the ch...
Bioorganic & Medicinal Chemistry Letters, 2014
A number of delivery agents, such as proteins, liposomes, micelles, and nanoparticles, are utiliz... more A number of delivery agents, such as proteins, liposomes, micelles, and nanoparticles, are utilized for transporting pharmaceutical agents in a physiological environment. This Letter focuses on the use of the copper(II) ion and its potential role as a delivery agent for the taxanes and taxol couple to a malaria drug. Nuclear magnetic resonance (NMR, (1)H, (13)C, (15)N), Mass Spectrometry (LC-MS, MALDI-TOF, FT-ICR) and computational methods are used to examine the structure of the complex. The National Cancer Institute's benchmark 60 cell line panel is used to compare the efficacy of the copper-taxol and copper-taxol-hydroxychloroquin complexes to that of iron-taxol and pure taxol.
Bioorganic & Medicinal Chemistry Letters, 2014
In recent years, the bacterium responsible for tuberculosis has been increasing its resistance to... more In recent years, the bacterium responsible for tuberculosis has been increasing its resistance to antibiotics resulting in new multidrug resistant Mycobacterium tuberculosis (MR-TB) and extensively drug-resistant tuberculosis (XDR-TB). In this study we use several analytical techniques including NMR, FT-ICR, TOF-MS, LC-MS and UV/Vis to study the copper-capreomycin complex. The copper (II) cation is used as a carrier for the antibiotic capreomycin. Once this structure was studied using NMR, FT-ICR, and MALDI-TOF-MS, the NIH-NIAID tuberculosis cell line for several Tb strains (including antibiotic resistant strains) were tested against up to seven variations of the copper-capreomycin complex. Different variations of copper improved the efficacy of capreomycin against Tb up to 250 fold against drug resistant strains of Tb.
International Journal of Mass Spectrometry, 2012
a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions ... more a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions was used to probe the structures of the YG a 2 ions generated from protonated YGGFL and doubly protonated YGGFLR. Our experiments indicate a mixture of cyclic and rearranged 'imine-amide' structures. The cyclic isomer is generated from the initially formed protonated imine terminated linear structure by head-to-tail cyclization. Proton transfer between the secondary amine of the ring and the amide nitrogen followed by ring opening leads to the rearranged 'imine-amide' isomer. Quantum chemical calculations demonstrate that this proton transfer is catalyzed by the tyrosine side chain ring for the YG a 2 ion. Isomer specific IRMPD bands observed in the two spectral regions clearly show the presence of the cyclic and rearranged 'imine-amide' isomers, the latter being characterized by an IR signature at ∼3545 cm −1 associated with the C-terminal amide NH 2 asymmetric stretch.
Journal of The American Society for Mass Spectrometry, 2012
The structure of the a 4 ion from protonated YGGFL was studied in a quadrupole ion trap mass spec... more The structure of the a 4 ion from protonated YGGFL was studied in a quadrupole ion trap mass spectrometer by 'action' infrared spectroscopy in the 1000-2000 cm -1 ('fingerprint') range using the CLIO Free Electron Laser. The potential energy surface (PES) of this ion was characterized by detailed molecular dynamics scans and density functional theory calculations exploring a large number of isomers and protonation sites. IR and theory indicate the a 4 ion population is primarily populated by the rearranged, linear structure proposed recently (Bythell et al., J. Am. Chem. Soc. 2010, 132, 14766). This structure contains an imine group at the N-terminus and an amide group -CO-NH 2 at the C-terminus. Our data also indicate that the originally proposed N-terminally protonated linear structure and macrocyclic structures (Polfer et al., J. Am. Chem. Soc. 2007, 129, 5887) are also present as minor populations. The clear differences between the present and previous IR spectra are discussed in detail. This mixture of gas-phase structures is also in agreement with the ion mobility spectrum published by Clemmer and co-workers recently (J. Phys. Chem. A 2008, 112, 1286. Additionally, the calculated cross-sections for the rearranged structures indicate these correspond to the most abundant (and previously unassigned) feature in Clemmer's work.
International Journal of Mass Spectrometry, 2012
a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions ... more a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions was used to probe the structures of the YG a 2 ions generated from protonated YGGFL and doubly protonated YGGFLR. Our experiments indicate a mixture of cyclic and rearranged 'imine-amide' structures. The cyclic isomer is generated from the initially formed protonated imine terminated linear structure by head-to-tail cyclization. Proton transfer between the secondary amine of the ring and the amide nitrogen followed by ring opening leads to the rearranged 'imine-amide' isomer. Quantum chemical calculations demonstrate that this proton transfer is catalyzed by the tyrosine side chain ring for the YG a 2 ion. Isomer specific IRMPD bands observed in the two spectral regions clearly show the presence of the cyclic and rearranged 'imine-amide' isomers, the latter being characterized by an IR signature at ∼3545 cm −1 associated with the C-terminal amide NH 2 asymmetric stretch.
Journal of the American Society for Mass Spectrometry, 2007
The fragmentation characteristics of protonated alanylglycylglycine, [AGG + H](+), were investiga... more The fragmentation characteristics of protonated alanylglycylglycine, [AGG + H](+), were investigated by tandem mass spectrometry in MALDI-TOF/TOF, ion trap, and hybrid sector instruments. b(2) is the most abundant fragment ion in MALDI-TOF/TOF, ion trap, and hybrid sector metastable ion (MI) experiments, while y(2) is slightly more abundant than b(2) in collision activated dissociation (CAD) performed in the sector instrument. The A-G amide bond is cleaved on the a(1)-y(2) pathway resulting in a proton-bound dimer of GG and MeCH=NH. Depending on the fragmentation conditions employed, this dimer can then (1) be detected as [AGG + H - CO](+), (2) dissociate to produce y(2) ions, [GG + H](+), (3) dissociate to produce a(1) ions, [MeCH=NH + H](+), or (4) rearrange to expel NH(3) forming a [AGG + H - CO - NH(3)](+) ion. The activation method and the experimental timescale employed largely dictate which of, and to what extent, these processes occur. These effects are qualitatively rationa...
The journal of physical chemistry. B, Jan 25, 2010
The gas-phase structures and fragmentation pathways of the singly protonated peptide arginylglycy... more The gas-phase structures and fragmentation pathways of the singly protonated peptide arginylglycylaspartic acid (RGD) are investigated by means of collision-induced-dissociation (CID) and detailed molecular mechanics and density functional theory (DFT) calculations. It is demonstrated that despite the ionizing proton being strongly sequestered at the guanidine group, protonated RGD can easily be fragmented on charge directed fragmentation pathways. This is due to facile mobilization of the C-terminal or aspartic acid COOH protons thereby generating salt-bridge (SB) stabilized structures. These SB intermediates can directly fragment to generate b(2) ions or facilely rearrange to form anhydrides from which both b(2) and b(2)+H(2)O fragments can be formed. The salt-bridge stabilized and anhydride transition structures (TSs) necessary to form b(2) and b(2)+H(2)O are much lower in energy than their traditional charge solvated counterparts. These mechanisms provide compelling evidence of ...
Journal of the American Society for Mass Spectrometry, 2012
We report the use of unimolecular dissociation by infrared radiation for gaseous multiphoton ener... more We report the use of unimolecular dissociation by infrared radiation for gaseous multiphoton energy transfer to determine relative activation energy (E(a,laser)) for dissociation of peptide sequence ions. The sequence ions of interest are mass-isolated; the entire ion cloud is then irradiated with a continuous wave CO(2) laser, and the first order rate constant, k(d), is determined for each of a series of laser powers. Provided these conditions are met, a plot of the natural logarithm of k(d) versus the natural logarithm of laser power yields a straight line, whose slope provides a measure of E(a,laser). This method reproduces the E(a) values from blackbody radiative dissociation (BIRD) for the comparatively large, singly and doubly protonated bradykinin ions (nominally y ( 9 ) and y ( 9 ) ( 2+ )). The comparatively small sequence ion systems produce E(a,laser) values that are systematic underestimates of theoretical barriers calculated with density functional theory (DFT). However,...
The journal of physical chemistry. C, Nanomaterials and interfaces, Jan 15, 2014
Graphene represents an attractive two-dimensional carbon-based nanomaterial that holds great prom... more Graphene represents an attractive two-dimensional carbon-based nanomaterial that holds great promise for applications such as electronics, batteries, sensors, and composite materials. Recent work has demonstrated that carbon-based nanomaterials are degradable/biodegradable, but little work has been expended to identify products formed during the degradation process. As these products may have toxicological implications that could leach into the environment or the human body, insight into the mechanism and structural elucidation remain important as carbon-based nanomaterials become commercialized. We provide insight into a potential mechanism of graphene oxide degradation via the photo-Fenton reaction. We have determined that after 1 day of treatment intermediate oxidation products (with MW 150-1000 Da) were generated. Upon longer reaction times (i.e., days 2 and 3), these products were no longer present in high abundance, and the system was dominated by graphene quantum dots (GQDs)....
The Journal of Physical Chemistry B, 2012
Infrared multiphoton dissociation (IRMPD) spectroscopy, using a free-electron laser, and ion mobi... more Infrared multiphoton dissociation (IRMPD) spectroscopy, using a free-electron laser, and ion mobility measurements, using both drift-cell and traveling-wave instruments, were used to investigate the structure of gas-phase peptide (AAHAL + 2H)(2+) ions produced by electrospray ionization. The experimental data from the IRMPD spectra and collisional cross section (Ω) measurements were consistent with the respective infrared spectra and Ω calculated for the lowest-energy peptide ion conformer obtained by extensive molecular dynamics searches and combined density functional theory and ab initio geometry optimizations and energy calculations. Traveling-wave ion mobility measurements were employed to obtain the Ω of charge-reduced peptide cation-radicals, (AAHAL + 2H)(+●), and the c(3), c(4), z(3), and z(4) fragments from electron-transfer dissociation (ETD) of (AAHAL + 2H)(2+). The experimental Ω for the ETD charge-reduced and fragment ions were consistent with the values calculated for fully optimized ion structures and indicated that the ions retained specific hydrogen bonding motifs from the precursor ion. In particular, the Ω for the doubly protonated ions and charge-reduced cation-radicals were nearly identical, indicating negligible unfolding and small secondary structure changes upon electron transfer. The experimental Ω for the (AAHAL + 2H)(+●) cation-radicals were compatible with both zwitterionic and histidine radical structures formed by electron attachment to different sites in the precursor ion, but did not allow their distinction. The best agreement with the experimental Ω was found for ion structures fully optimized with M06-2X/6-31+G(d,p) and using both projection approximation and trajectory methods to calculate the theoretical Ω values.
The Journal of Physical Chemistry A, 2010
Multiple-stage tandem mass spectrometry and collision-induced dissociation were used to investiga... more Multiple-stage tandem mass spectrometry and collision-induced dissociation were used to investigate loss of H 2 O or CH 3 OH from protonated versions of GGGX (where X ) G, A, and V), GGGGG, and the methyl esters of these peptides. In addition, wavelength-selective infrared multiple photon dissociation was used to characterize the [M + H -H 2 O] + product derived from protonated GGGG and the major MS 3 fragment, [M + H -H 2 O -29] + of this peak. Consistent with the earlier work [Ballard, K. D.; Gaskell, S. J. J. Am. Soc. Mass Spectrom. 1993, 4, 477-481; Reid, G. E.; Simpson, R. J.; O'Hair, R. A. J. Int. J. Mass Spectrom. 1999, 190/191, 209-230000], CID experiments show that [M + H -H 2 O] + is the dominant peak generated from both protonated GGGG and protonated GGGG-OMe. This strongly suggests that the loss of the H 2 O molecule occurs from a position other than the C-terminal free acid and that the product does not correspond to formation of the b 4 ion. Subsequent CID of [M + H -H 2 O] + supports this proposal by resulting in a major product that is 29 mass units less than the precursor ion. This is consistent with loss of HNdCH 2 rather than loss of carbon monoxide (28 mass units), which is characteristic of oxazolone-type b n ions. Comparison between experimental and theoretical infrared spectra for a group of possible structures confirms that the [M + H -H 2 O] + peak is not a substituted oxazolone but instead suggests formation of an ion that features a five-membered ring along the peptide backbone, close to the amino terminus. Additionally, transition structure calculations and comparison of theoretical and experimental spectra of the [M + H -H 2 O -29] + peak also support this proposal.
Journal of The American Society for Mass Spectrometry, 2011
Charge-directed fragmentation has been shown to be the prevalent dissociation step for protonated... more Charge-directed fragmentation has been shown to be the prevalent dissociation step for protonated peptides under the low-energy activation (eV) regime. Thus, the determination of the ion structure and, in particular, the characterization of the protonation site(s) of peptides and their fragments is a key approach to substantiate and refine peptide fragmentation mechanisms. Here we report on the characterization of the protonation site of oxazolone b 2 ions formed in collision-induced dissociation (CID) of the doubly protonated tryptic model-peptide YIGSR. In support of earlier work, here we provide complementary IR spectra in the 2800-3800 cm -1 range acquired on a table-top laser system. Combining this tunable laser with a high power CO 2 laser to improve spectroscopic sensitivity, well resolved bands are observed, with an excellent correspondence to the IR absorption bands of the ring-protonated oxazolone isomer as predicted by quantum chemical calculations. In particular, it is shown that a band at 3445 cm -1 , corresponding to the asymmetric N-H stretch of the (nonprotonated) N-terminal NH 2 group, is a distinct vibrational signature of the ring-protonated oxazolone structure.
Journal of the American Society for Mass Spectrometry, 2009
H/D exchange and theoretical calculations elucidate the structure of peptide fragments from doubl... more H/D exchange and theoretical calculations elucidate the structure of peptide fragments from doubly protonated tryptic peptides.
Journal of the American Society for Mass Spectrometry, 2008
It has been determined experimentally that a 3 ions are generally not observed in the tandem mass... more It has been determined experimentally that a 3 ions are generally not observed in the tandem mass spectroscopic (MS/MS) spectra of b 3 ions. This is in contrast to other b n ions, which often have the corresponding a n ion as the base peak in their MS/MS spectra. Although this might suggest a different structure for b 3 ions compared to that of other b n ions, theoretical calculations indicate the conventional oxazolone structure to be the lowest energy structure for the b 3 ion of AAAAR, as it is for other b n ions of this peptide. However, it has been determined theoretically that the a 3 ion is lower in energy than other a n ions, relative to the corresponding b ions. Furthermore, the a 3 ¡ b 2 transition structure (TS) is lower in energy than other a n ¡ b nϪ1 TSs of AAAAR, compared with the corresponding b ions. Consequently, it is suggested that the b 3 ion does fragment to the a 3 ion, but that the a 3 ion then immediately fragments (to b 2 and a 3 *) because of the excess internal energy arising from its relatively low energy and the facile a 3 ¡ b 2 reaction. That is why a 3 ions are not observed in the MS/MS spectra of b 3 ions. (J Am T andem mass spectrometry (MS/MS) is by far the most common method for identifying peptides and proteins. Characteristic fragmentations occur along the polypeptide backbone, depending on the ion activation method used. "Slow heating" techniques, such as low-energy collision-induced-dissociation (CID) and infrared multiphoton dissociation (IRMPD), typically cause cleavage at the peptide bond forming b, a, and y ions [1, 2]. Conversely, electron capture dissociation (ECD) cleaves the NOC ␣ bond to form c and z ions . There is a long history of studying the mechanisms of dissociation of peptide ions, originally concentrating on formation of b-, a-, and y-type ions, [2, 5-17] but more recently the formation of c and z ions [18 -22].
Journal of the American Society for Mass Spectrometry, 2007
The fragmentation characteristics of protonated alanylglycylglycine, [AGG ϩ H] ϩ , were investiga... more The fragmentation characteristics of protonated alanylglycylglycine, [AGG ϩ H] ϩ , were investigated by tandem mass spectrometry in MALDI-TOF/TOF, ion trap, and hybrid sector instruments. b 2 is the most abundant fragment ion in MALDI-TOF/TOF, ion trap, and hybrid sector metastable ion (MI) experiments, while y 2 is slightly more abundant than b 2 in collision activated dissociation (CAD) performed in the sector instrument. The A-G amide bond is cleaved on the a 1 -y 2 pathway resulting in a proton-bound dimer of GG and MeCHϭNH. Depending on the fragmentation conditions employed, this dimer can then (1) be detected as [AGG ϩ H Ϫ CO] ϩ , (2) dissociate to produce y 2 ions, [GG ϩ H] ϩ , (3) dissociate to produce a 1 ions, [MeCHϭNH ϩ H] ϩ , or (4) rearrange to expel NH 3 forming a [AGG ϩ H Ϫ CO Ϫ NH 3 ] ϩ ion. The activation method and the experimental timescale employed largely dictate which of, and to what extent, these processes occur. These effects are qualitatively rationalized with the help of quantum chemical and RRKM calculations. Two mechanisms for formation of the [AGG ϩ H Ϫ CO Ϫ NH 3 ] ϩ ion were evaluated through nitrogen-15 labeling experiments and quantum chemical calculations. A mechanism involving intermolecular nucleophilic attack and association of the GG and imine fragments followed by ammonia loss was found to be more energetically favorable than expulsion of ammonia in an S N 2-type reaction. (J Am Soc
Journal of the American Society for Mass Spectrometry, 2008
Extensive 15 N labeling and multiple-stage tandem mass spectrometry were used to investigate the ... more Extensive 15 N labeling and multiple-stage tandem mass spectrometry were used to investigate the fragmentation pathways of the model peptide FGGFL during low-energy collisioninduced-dissociation (CID) in an ion-trap mass spectrometer. Of particular interest was formation of a 4 from b 4 and a 4 * (a 4 -NH 3 ) from a 4 ions correspondingly, and apparent rearrangement and scrambling of peptide sequence during CID. It is suggested that the original FGGF oxa b 4 structure undergoes b-type scrambling to form GGFF oxa . These two isomers fragment further by elimination of CO and 14 NH 3 or 15 NH 3 to form the corresponding a 4 and a 4 * isomers, respectively. For ( 15 N-F)GGFL and FGG( 15 N-F)L the a 4 * ion population appears as two distinct peaks separated by 1 mass unit. These two peaks could be separated and fragmented individually in subsequent CID stages to provide a useful tool for exploration of potential mechanisms along the a 4 ¡ a 4 * pathway reported previously in the literature (Vachet et al.
Journal of the American Chemical Society, 2009
The mobile proton model (Dongre, A. R., Jones, J. L., Somogyi, A. and Wysocki, V. H. J. Am. Chem.... more The mobile proton model (Dongre, A. R., Jones, J. L., Somogyi, A. and Wysocki, V. H. J. Am. Chem. Soc. 1996, 118 , 8365-8374) of peptide fragmentation states that the ionizing protons play a critical role in the gas-phase fragmentation of protonated peptides upon collision-induced dissociation (CID). The model distinguishes two classes of peptide ions, those with or without easily mobilizable protons. For the former class mild excitation leads to proton transfer reactions which populate amide nitrogen protonation sites. This enables facile amide bond cleavage and thus the formation of b and y sequence ions. In contrast, the latter class of peptide ions contains strongly basic functionalities which sequester the ionizing protons, thereby often hindering formation of sequence ions. Here we describe the proton-driven amide bond cleavages necessary to produce b and y ions from peptide ions lacking easily mobilizable protons. We show that this important class of peptide ions fragments by different means from those with easily mobilizable protons. We present three new amide bond cleavage mechanisms which involve salt-bridge, anhydride, and imine enol intermediates, respectively. All three new mechanisms are less energetically demanding than the classical oxazolone b(n)-y(m) pathway. These mechanisms offer an explanation for the formation of b and y ions from peptide ions with sequestered ionizing protons which are routinely fragmented in large-scale proteomics experiments.
Journal of the American Chemical Society, 2009
b ions are of fundamental importance in peptide sequencing using tandem mass spectrometry. These ... more b ions are of fundamental importance in peptide sequencing using tandem mass spectrometry. These ions have generally been assumed to exist as protonated oxazolone derivatives. Recent work indicates that medium-sized b ions can rearrange by head-to-tail cyclization of the oxazolone structures generating macrocyclic protonated peptides as intermediates. Here, we show using infrared spectroscopy and density functional theory calculations that the b 5 ion of protonated G 5 R exists in the mass spectrometer as an amide oxygen protonated cyclic peptide rather than fleetingly as a transient intermediate. This assignment is supported by our DFT calculations which show this macrocyclic isomer to be energetically preferred over the open oxazolone form despite the entropic constraints the cyclic form introduces.
Journal of the American Chemical Society, 2010
Electron-transfer and -capture dissociations of doubly protonated peptides gave dramatically diff... more Electron-transfer and -capture dissociations of doubly protonated peptides gave dramatically different product ions for a series of histidine-containing pentapeptides of both non-tryptic (AAHAL, AHAAL, AHADL, AHDAL) and tryptic (AAAHK, AAHAK, AHAAK, HAAAK, AAAHR, AAHAR, AHAAR, HAAAR) type. Electron transfer from gaseous Cs atoms and fluoranthene anions triggered backbone dissociations of all four N-C(alpha) bonds in the peptide ions in addition to loss of H and NH(3). Substantial fractions of charge-reduced cation-radicals did not dissociate on an experimental time scale ranging from 10(-6) to 10(-1) s. Multistage tandem mass spectrometric (MS(n)) experiments indicated that the non-dissociating cation-radicals had undergone rearrangements. These were explained as being due to proton migrations from N-terminal ammonium and COOH groups to the C-2' position of the reduced His ring, resulting in substantial radical stabilization. Ab initio calculations revealed that the charge-reduced cation-radicals can exist as low-energy zwitterionic amide pi* states which were local energy minima. These states underwent facile exothermic proton migrations to form aminoketyl radical intermediates, whereas direct N-C(alpha) bond cleavage in zwitterions was disfavored. RRKM analysis indicated that backbone N-C(alpha) bond cleavages did not occur competitively from a single charge-reduced precursor. Rather, these bond cleavages proceeded from distinct intermediates which originated from different electronic states accessed by electron transfer. In stark contrast to electron transfer, capture of a free electron by the peptide ions mainly induced radical dissociations of the charge-carrying side chains and loss of a hydrogen atom followed by standard backbone dissociations of even-electron ions. The differences in dissociation are explained by different electronic states being accessed upon electron transfer and capture.
The journal of physical chemistry. A, Jan 13, 2014
Recently, I explored structurally straightforward pathways to Cα hydrogen atom, H(•), transfer re... more Recently, I explored structurally straightforward pathways to Cα hydrogen atom, H(•), transfer reactions in the radical cation complex following electron capture/transfer of a series of polyprotonated peptides (J. Phys. Chem. A 2013, 117, 1189-1196). Here, I extend my analysis to incorporate detailed rearrangement processes potentially occurring prior to H(•) transfer. This comprises intracomplex isomerization of the initial iminol-terminated (-C(OH)═NH) form of the cn' species to the energetically more favorable, amide-terminated form (-C(O)-NH2) prior to Cα H(•) abstraction by the zm(•) species. The data indicate that the previously published H(•) transfer barriers are more energetically demanding than those of this multistep alternative. The rate-determining step is typically the intracomplex iminol isomerization, consistent with the substantial energetic favorability of the amide form of the cn species. The barriers to H(•) transfer still rise steeply as a function of the ch...
Bioorganic & Medicinal Chemistry Letters, 2014
A number of delivery agents, such as proteins, liposomes, micelles, and nanoparticles, are utiliz... more A number of delivery agents, such as proteins, liposomes, micelles, and nanoparticles, are utilized for transporting pharmaceutical agents in a physiological environment. This Letter focuses on the use of the copper(II) ion and its potential role as a delivery agent for the taxanes and taxol couple to a malaria drug. Nuclear magnetic resonance (NMR, (1)H, (13)C, (15)N), Mass Spectrometry (LC-MS, MALDI-TOF, FT-ICR) and computational methods are used to examine the structure of the complex. The National Cancer Institute's benchmark 60 cell line panel is used to compare the efficacy of the copper-taxol and copper-taxol-hydroxychloroquin complexes to that of iron-taxol and pure taxol.
Bioorganic & Medicinal Chemistry Letters, 2014
In recent years, the bacterium responsible for tuberculosis has been increasing its resistance to... more In recent years, the bacterium responsible for tuberculosis has been increasing its resistance to antibiotics resulting in new multidrug resistant Mycobacterium tuberculosis (MR-TB) and extensively drug-resistant tuberculosis (XDR-TB). In this study we use several analytical techniques including NMR, FT-ICR, TOF-MS, LC-MS and UV/Vis to study the copper-capreomycin complex. The copper (II) cation is used as a carrier for the antibiotic capreomycin. Once this structure was studied using NMR, FT-ICR, and MALDI-TOF-MS, the NIH-NIAID tuberculosis cell line for several Tb strains (including antibiotic resistant strains) were tested against up to seven variations of the copper-capreomycin complex. Different variations of copper improved the efficacy of capreomycin against Tb up to 250 fold against drug resistant strains of Tb.
International Journal of Mass Spectrometry, 2012
a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions ... more a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions was used to probe the structures of the YG a 2 ions generated from protonated YGGFL and doubly protonated YGGFLR. Our experiments indicate a mixture of cyclic and rearranged 'imine-amide' structures. The cyclic isomer is generated from the initially formed protonated imine terminated linear structure by head-to-tail cyclization. Proton transfer between the secondary amine of the ring and the amide nitrogen followed by ring opening leads to the rearranged 'imine-amide' isomer. Quantum chemical calculations demonstrate that this proton transfer is catalyzed by the tyrosine side chain ring for the YG a 2 ion. Isomer specific IRMPD bands observed in the two spectral regions clearly show the presence of the cyclic and rearranged 'imine-amide' isomers, the latter being characterized by an IR signature at ∼3545 cm −1 associated with the C-terminal amide NH 2 asymmetric stretch.
Journal of The American Society for Mass Spectrometry, 2012
The structure of the a 4 ion from protonated YGGFL was studied in a quadrupole ion trap mass spec... more The structure of the a 4 ion from protonated YGGFL was studied in a quadrupole ion trap mass spectrometer by 'action' infrared spectroscopy in the 1000-2000 cm -1 ('fingerprint') range using the CLIO Free Electron Laser. The potential energy surface (PES) of this ion was characterized by detailed molecular dynamics scans and density functional theory calculations exploring a large number of isomers and protonation sites. IR and theory indicate the a 4 ion population is primarily populated by the rearranged, linear structure proposed recently (Bythell et al., J. Am. Chem. Soc. 2010, 132, 14766). This structure contains an imine group at the N-terminus and an amide group -CO-NH 2 at the C-terminus. Our data also indicate that the originally proposed N-terminally protonated linear structure and macrocyclic structures (Polfer et al., J. Am. Chem. Soc. 2007, 129, 5887) are also present as minor populations. The clear differences between the present and previous IR spectra are discussed in detail. This mixture of gas-phase structures is also in agreement with the ion mobility spectrum published by Clemmer and co-workers recently (J. Phys. Chem. A 2008, 112, 1286. Additionally, the calculated cross-sections for the rearranged structures indicate these correspond to the most abundant (and previously unassigned) feature in Clemmer's work.
International Journal of Mass Spectrometry, 2012
a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions ... more a b s t r a c t IRMPD spectroscopy in the 'fingerprint' and X H (X = C, N, O) stretching regions was used to probe the structures of the YG a 2 ions generated from protonated YGGFL and doubly protonated YGGFLR. Our experiments indicate a mixture of cyclic and rearranged 'imine-amide' structures. The cyclic isomer is generated from the initially formed protonated imine terminated linear structure by head-to-tail cyclization. Proton transfer between the secondary amine of the ring and the amide nitrogen followed by ring opening leads to the rearranged 'imine-amide' isomer. Quantum chemical calculations demonstrate that this proton transfer is catalyzed by the tyrosine side chain ring for the YG a 2 ion. Isomer specific IRMPD bands observed in the two spectral regions clearly show the presence of the cyclic and rearranged 'imine-amide' isomers, the latter being characterized by an IR signature at ∼3545 cm −1 associated with the C-terminal amide NH 2 asymmetric stretch.
Journal of the American Society for Mass Spectrometry, 2007
The fragmentation characteristics of protonated alanylglycylglycine, [AGG + H](+), were investiga... more The fragmentation characteristics of protonated alanylglycylglycine, [AGG + H](+), were investigated by tandem mass spectrometry in MALDI-TOF/TOF, ion trap, and hybrid sector instruments. b(2) is the most abundant fragment ion in MALDI-TOF/TOF, ion trap, and hybrid sector metastable ion (MI) experiments, while y(2) is slightly more abundant than b(2) in collision activated dissociation (CAD) performed in the sector instrument. The A-G amide bond is cleaved on the a(1)-y(2) pathway resulting in a proton-bound dimer of GG and MeCH=NH. Depending on the fragmentation conditions employed, this dimer can then (1) be detected as [AGG + H - CO](+), (2) dissociate to produce y(2) ions, [GG + H](+), (3) dissociate to produce a(1) ions, [MeCH=NH + H](+), or (4) rearrange to expel NH(3) forming a [AGG + H - CO - NH(3)](+) ion. The activation method and the experimental timescale employed largely dictate which of, and to what extent, these processes occur. These effects are qualitatively rationa...
The journal of physical chemistry. B, Jan 25, 2010
The gas-phase structures and fragmentation pathways of the singly protonated peptide arginylglycy... more The gas-phase structures and fragmentation pathways of the singly protonated peptide arginylglycylaspartic acid (RGD) are investigated by means of collision-induced-dissociation (CID) and detailed molecular mechanics and density functional theory (DFT) calculations. It is demonstrated that despite the ionizing proton being strongly sequestered at the guanidine group, protonated RGD can easily be fragmented on charge directed fragmentation pathways. This is due to facile mobilization of the C-terminal or aspartic acid COOH protons thereby generating salt-bridge (SB) stabilized structures. These SB intermediates can directly fragment to generate b(2) ions or facilely rearrange to form anhydrides from which both b(2) and b(2)+H(2)O fragments can be formed. The salt-bridge stabilized and anhydride transition structures (TSs) necessary to form b(2) and b(2)+H(2)O are much lower in energy than their traditional charge solvated counterparts. These mechanisms provide compelling evidence of ...
Journal of the American Society for Mass Spectrometry, 2012
We report the use of unimolecular dissociation by infrared radiation for gaseous multiphoton ener... more We report the use of unimolecular dissociation by infrared radiation for gaseous multiphoton energy transfer to determine relative activation energy (E(a,laser)) for dissociation of peptide sequence ions. The sequence ions of interest are mass-isolated; the entire ion cloud is then irradiated with a continuous wave CO(2) laser, and the first order rate constant, k(d), is determined for each of a series of laser powers. Provided these conditions are met, a plot of the natural logarithm of k(d) versus the natural logarithm of laser power yields a straight line, whose slope provides a measure of E(a,laser). This method reproduces the E(a) values from blackbody radiative dissociation (BIRD) for the comparatively large, singly and doubly protonated bradykinin ions (nominally y ( 9 ) and y ( 9 ) ( 2+ )). The comparatively small sequence ion systems produce E(a,laser) values that are systematic underestimates of theoretical barriers calculated with density functional theory (DFT). However,...
The journal of physical chemistry. C, Nanomaterials and interfaces, Jan 15, 2014
Graphene represents an attractive two-dimensional carbon-based nanomaterial that holds great prom... more Graphene represents an attractive two-dimensional carbon-based nanomaterial that holds great promise for applications such as electronics, batteries, sensors, and composite materials. Recent work has demonstrated that carbon-based nanomaterials are degradable/biodegradable, but little work has been expended to identify products formed during the degradation process. As these products may have toxicological implications that could leach into the environment or the human body, insight into the mechanism and structural elucidation remain important as carbon-based nanomaterials become commercialized. We provide insight into a potential mechanism of graphene oxide degradation via the photo-Fenton reaction. We have determined that after 1 day of treatment intermediate oxidation products (with MW 150-1000 Da) were generated. Upon longer reaction times (i.e., days 2 and 3), these products were no longer present in high abundance, and the system was dominated by graphene quantum dots (GQDs)....
The Journal of Physical Chemistry B, 2012
Infrared multiphoton dissociation (IRMPD) spectroscopy, using a free-electron laser, and ion mobi... more Infrared multiphoton dissociation (IRMPD) spectroscopy, using a free-electron laser, and ion mobility measurements, using both drift-cell and traveling-wave instruments, were used to investigate the structure of gas-phase peptide (AAHAL + 2H)(2+) ions produced by electrospray ionization. The experimental data from the IRMPD spectra and collisional cross section (Ω) measurements were consistent with the respective infrared spectra and Ω calculated for the lowest-energy peptide ion conformer obtained by extensive molecular dynamics searches and combined density functional theory and ab initio geometry optimizations and energy calculations. Traveling-wave ion mobility measurements were employed to obtain the Ω of charge-reduced peptide cation-radicals, (AAHAL + 2H)(+●), and the c(3), c(4), z(3), and z(4) fragments from electron-transfer dissociation (ETD) of (AAHAL + 2H)(2+). The experimental Ω for the ETD charge-reduced and fragment ions were consistent with the values calculated for fully optimized ion structures and indicated that the ions retained specific hydrogen bonding motifs from the precursor ion. In particular, the Ω for the doubly protonated ions and charge-reduced cation-radicals were nearly identical, indicating negligible unfolding and small secondary structure changes upon electron transfer. The experimental Ω for the (AAHAL + 2H)(+●) cation-radicals were compatible with both zwitterionic and histidine radical structures formed by electron attachment to different sites in the precursor ion, but did not allow their distinction. The best agreement with the experimental Ω was found for ion structures fully optimized with M06-2X/6-31+G(d,p) and using both projection approximation and trajectory methods to calculate the theoretical Ω values.
The Journal of Physical Chemistry A, 2010
Multiple-stage tandem mass spectrometry and collision-induced dissociation were used to investiga... more Multiple-stage tandem mass spectrometry and collision-induced dissociation were used to investigate loss of H 2 O or CH 3 OH from protonated versions of GGGX (where X ) G, A, and V), GGGGG, and the methyl esters of these peptides. In addition, wavelength-selective infrared multiple photon dissociation was used to characterize the [M + H -H 2 O] + product derived from protonated GGGG and the major MS 3 fragment, [M + H -H 2 O -29] + of this peak. Consistent with the earlier work [Ballard, K. D.; Gaskell, S. J. J. Am. Soc. Mass Spectrom. 1993, 4, 477-481; Reid, G. E.; Simpson, R. J.; O'Hair, R. A. J. Int. J. Mass Spectrom. 1999, 190/191, 209-230000], CID experiments show that [M + H -H 2 O] + is the dominant peak generated from both protonated GGGG and protonated GGGG-OMe. This strongly suggests that the loss of the H 2 O molecule occurs from a position other than the C-terminal free acid and that the product does not correspond to formation of the b 4 ion. Subsequent CID of [M + H -H 2 O] + supports this proposal by resulting in a major product that is 29 mass units less than the precursor ion. This is consistent with loss of HNdCH 2 rather than loss of carbon monoxide (28 mass units), which is characteristic of oxazolone-type b n ions. Comparison between experimental and theoretical infrared spectra for a group of possible structures confirms that the [M + H -H 2 O] + peak is not a substituted oxazolone but instead suggests formation of an ion that features a five-membered ring along the peptide backbone, close to the amino terminus. Additionally, transition structure calculations and comparison of theoretical and experimental spectra of the [M + H -H 2 O -29] + peak also support this proposal.
Journal of The American Society for Mass Spectrometry, 2011
Charge-directed fragmentation has been shown to be the prevalent dissociation step for protonated... more Charge-directed fragmentation has been shown to be the prevalent dissociation step for protonated peptides under the low-energy activation (eV) regime. Thus, the determination of the ion structure and, in particular, the characterization of the protonation site(s) of peptides and their fragments is a key approach to substantiate and refine peptide fragmentation mechanisms. Here we report on the characterization of the protonation site of oxazolone b 2 ions formed in collision-induced dissociation (CID) of the doubly protonated tryptic model-peptide YIGSR. In support of earlier work, here we provide complementary IR spectra in the 2800-3800 cm -1 range acquired on a table-top laser system. Combining this tunable laser with a high power CO 2 laser to improve spectroscopic sensitivity, well resolved bands are observed, with an excellent correspondence to the IR absorption bands of the ring-protonated oxazolone isomer as predicted by quantum chemical calculations. In particular, it is shown that a band at 3445 cm -1 , corresponding to the asymmetric N-H stretch of the (nonprotonated) N-terminal NH 2 group, is a distinct vibrational signature of the ring-protonated oxazolone structure.
Journal of the American Society for Mass Spectrometry, 2009
H/D exchange and theoretical calculations elucidate the structure of peptide fragments from doubl... more H/D exchange and theoretical calculations elucidate the structure of peptide fragments from doubly protonated tryptic peptides.
Journal of the American Society for Mass Spectrometry, 2008
It has been determined experimentally that a 3 ions are generally not observed in the tandem mass... more It has been determined experimentally that a 3 ions are generally not observed in the tandem mass spectroscopic (MS/MS) spectra of b 3 ions. This is in contrast to other b n ions, which often have the corresponding a n ion as the base peak in their MS/MS spectra. Although this might suggest a different structure for b 3 ions compared to that of other b n ions, theoretical calculations indicate the conventional oxazolone structure to be the lowest energy structure for the b 3 ion of AAAAR, as it is for other b n ions of this peptide. However, it has been determined theoretically that the a 3 ion is lower in energy than other a n ions, relative to the corresponding b ions. Furthermore, the a 3 ¡ b 2 transition structure (TS) is lower in energy than other a n ¡ b nϪ1 TSs of AAAAR, compared with the corresponding b ions. Consequently, it is suggested that the b 3 ion does fragment to the a 3 ion, but that the a 3 ion then immediately fragments (to b 2 and a 3 *) because of the excess internal energy arising from its relatively low energy and the facile a 3 ¡ b 2 reaction. That is why a 3 ions are not observed in the MS/MS spectra of b 3 ions. (J Am T andem mass spectrometry (MS/MS) is by far the most common method for identifying peptides and proteins. Characteristic fragmentations occur along the polypeptide backbone, depending on the ion activation method used. "Slow heating" techniques, such as low-energy collision-induced-dissociation (CID) and infrared multiphoton dissociation (IRMPD), typically cause cleavage at the peptide bond forming b, a, and y ions [1, 2]. Conversely, electron capture dissociation (ECD) cleaves the NOC ␣ bond to form c and z ions . There is a long history of studying the mechanisms of dissociation of peptide ions, originally concentrating on formation of b-, a-, and y-type ions, [2, 5-17] but more recently the formation of c and z ions [18 -22].
Journal of the American Society for Mass Spectrometry, 2007
The fragmentation characteristics of protonated alanylglycylglycine, [AGG ϩ H] ϩ , were investiga... more The fragmentation characteristics of protonated alanylglycylglycine, [AGG ϩ H] ϩ , were investigated by tandem mass spectrometry in MALDI-TOF/TOF, ion trap, and hybrid sector instruments. b 2 is the most abundant fragment ion in MALDI-TOF/TOF, ion trap, and hybrid sector metastable ion (MI) experiments, while y 2 is slightly more abundant than b 2 in collision activated dissociation (CAD) performed in the sector instrument. The A-G amide bond is cleaved on the a 1 -y 2 pathway resulting in a proton-bound dimer of GG and MeCHϭNH. Depending on the fragmentation conditions employed, this dimer can then (1) be detected as [AGG ϩ H Ϫ CO] ϩ , (2) dissociate to produce y 2 ions, [GG ϩ H] ϩ , (3) dissociate to produce a 1 ions, [MeCHϭNH ϩ H] ϩ , or (4) rearrange to expel NH 3 forming a [AGG ϩ H Ϫ CO Ϫ NH 3 ] ϩ ion. The activation method and the experimental timescale employed largely dictate which of, and to what extent, these processes occur. These effects are qualitatively rationalized with the help of quantum chemical and RRKM calculations. Two mechanisms for formation of the [AGG ϩ H Ϫ CO Ϫ NH 3 ] ϩ ion were evaluated through nitrogen-15 labeling experiments and quantum chemical calculations. A mechanism involving intermolecular nucleophilic attack and association of the GG and imine fragments followed by ammonia loss was found to be more energetically favorable than expulsion of ammonia in an S N 2-type reaction. (J Am Soc
Journal of the American Society for Mass Spectrometry, 2008
Extensive 15 N labeling and multiple-stage tandem mass spectrometry were used to investigate the ... more Extensive 15 N labeling and multiple-stage tandem mass spectrometry were used to investigate the fragmentation pathways of the model peptide FGGFL during low-energy collisioninduced-dissociation (CID) in an ion-trap mass spectrometer. Of particular interest was formation of a 4 from b 4 and a 4 * (a 4 -NH 3 ) from a 4 ions correspondingly, and apparent rearrangement and scrambling of peptide sequence during CID. It is suggested that the original FGGF oxa b 4 structure undergoes b-type scrambling to form GGFF oxa . These two isomers fragment further by elimination of CO and 14 NH 3 or 15 NH 3 to form the corresponding a 4 and a 4 * isomers, respectively. For ( 15 N-F)GGFL and FGG( 15 N-F)L the a 4 * ion population appears as two distinct peaks separated by 1 mass unit. These two peaks could be separated and fragmented individually in subsequent CID stages to provide a useful tool for exploration of potential mechanisms along the a 4 ¡ a 4 * pathway reported previously in the literature (Vachet et al.
Journal of the American Chemical Society, 2009
The mobile proton model (Dongre, A. R., Jones, J. L., Somogyi, A. and Wysocki, V. H. J. Am. Chem.... more The mobile proton model (Dongre, A. R., Jones, J. L., Somogyi, A. and Wysocki, V. H. J. Am. Chem. Soc. 1996, 118 , 8365-8374) of peptide fragmentation states that the ionizing protons play a critical role in the gas-phase fragmentation of protonated peptides upon collision-induced dissociation (CID). The model distinguishes two classes of peptide ions, those with or without easily mobilizable protons. For the former class mild excitation leads to proton transfer reactions which populate amide nitrogen protonation sites. This enables facile amide bond cleavage and thus the formation of b and y sequence ions. In contrast, the latter class of peptide ions contains strongly basic functionalities which sequester the ionizing protons, thereby often hindering formation of sequence ions. Here we describe the proton-driven amide bond cleavages necessary to produce b and y ions from peptide ions lacking easily mobilizable protons. We show that this important class of peptide ions fragments by different means from those with easily mobilizable protons. We present three new amide bond cleavage mechanisms which involve salt-bridge, anhydride, and imine enol intermediates, respectively. All three new mechanisms are less energetically demanding than the classical oxazolone b(n)-y(m) pathway. These mechanisms offer an explanation for the formation of b and y ions from peptide ions with sequestered ionizing protons which are routinely fragmented in large-scale proteomics experiments.
Journal of the American Chemical Society, 2009
b ions are of fundamental importance in peptide sequencing using tandem mass spectrometry. These ... more b ions are of fundamental importance in peptide sequencing using tandem mass spectrometry. These ions have generally been assumed to exist as protonated oxazolone derivatives. Recent work indicates that medium-sized b ions can rearrange by head-to-tail cyclization of the oxazolone structures generating macrocyclic protonated peptides as intermediates. Here, we show using infrared spectroscopy and density functional theory calculations that the b 5 ion of protonated G 5 R exists in the mass spectrometer as an amide oxygen protonated cyclic peptide rather than fleetingly as a transient intermediate. This assignment is supported by our DFT calculations which show this macrocyclic isomer to be energetically preferred over the open oxazolone form despite the entropic constraints the cyclic form introduces.
Journal of the American Chemical Society, 2010
Electron-transfer and -capture dissociations of doubly protonated peptides gave dramatically diff... more Electron-transfer and -capture dissociations of doubly protonated peptides gave dramatically different product ions for a series of histidine-containing pentapeptides of both non-tryptic (AAHAL, AHAAL, AHADL, AHDAL) and tryptic (AAAHK, AAHAK, AHAAK, HAAAK, AAAHR, AAHAR, AHAAR, HAAAR) type. Electron transfer from gaseous Cs atoms and fluoranthene anions triggered backbone dissociations of all four N-C(alpha) bonds in the peptide ions in addition to loss of H and NH(3). Substantial fractions of charge-reduced cation-radicals did not dissociate on an experimental time scale ranging from 10(-6) to 10(-1) s. Multistage tandem mass spectrometric (MS(n)) experiments indicated that the non-dissociating cation-radicals had undergone rearrangements. These were explained as being due to proton migrations from N-terminal ammonium and COOH groups to the C-2' position of the reduced His ring, resulting in substantial radical stabilization. Ab initio calculations revealed that the charge-reduced cation-radicals can exist as low-energy zwitterionic amide pi* states which were local energy minima. These states underwent facile exothermic proton migrations to form aminoketyl radical intermediates, whereas direct N-C(alpha) bond cleavage in zwitterions was disfavored. RRKM analysis indicated that backbone N-C(alpha) bond cleavages did not occur competitively from a single charge-reduced precursor. Rather, these bond cleavages proceeded from distinct intermediates which originated from different electronic states accessed by electron transfer. In stark contrast to electron transfer, capture of a free electron by the peptide ions mainly induced radical dissociations of the charge-carrying side chains and loss of a hydrogen atom followed by standard backbone dissociations of even-electron ions. The differences in dissociation are explained by different electronic states being accessed upon electron transfer and capture.