Adeniyi Ohunayo | Ekiti State University, Ado-Ekiti, Nigeria (original) (raw)
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INDIAN AGRICULTURAL RESEARCH INSTITUTE, NEW DELHI, INDIA
University Of Veterinary And Animal Sciences Lahore
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Papers by Adeniyi Ohunayo
CRC Press eBooks, Jan 16, 2024
OA Molecular and Cell Biology, 2013
Therapeutic Proteins Against Human Diseases, 2022
Synbiotics for the Management of Cancer, 2023
Journal of Advances in Biology & Biotechnology, Nov 17, 2022
Klebsiella species are one of the major causes of systematic infections, theses gram negative org... more Klebsiella species are one of the major causes of systematic infections, theses gram negative organisms are also capable of housing various resistant genes to resist the potency of conventional antibiotics. These observations account for the need to assess the antibacterial activity of Gentamicin and natural products against Klebsiella. A total of fifty (50) clinical isolates of Klebsiella species of different pathological sources were collected from four different Teaching Hospitals in Southwest, Nigeria. The identification of all the isolates was done using conventional biochemical tests. Antibiogram was carried out on all 50 Klebsiella clinical isolates using multiple antibiotic discs and the sensitivity of honey was done using the agar diffusion method. In the antimicrobial susceptibility test on honey, two undiluted different samples of honey (Honey A Refined and Honey B Natural) showed high activity and 1:2 to 1:6 aq. dilutions showed less activity against the Klebsiella isolates. Gentamicin used at the concentration of 4.0µg/ml has great activity against the isolates but was basically lower than the antibacterial activity of each undiluted honey. In the occasion of therapeutic disaster with gentamicin or any other associated antibiotics, honey offers an appropriate and improved alternative in dealing with infected burn wounds and other infections like urinary tract infections, nosocomial infections, etc.
Therapeutic Proteins Against Human Diseases, 2022
Handbook of Animal Models and its Uses in Cancer Research, 2022
Microbiome in Inflammatory Lung Diseases, 2022
Plasmid curing of microbes and physicochemical analysis of water samples obtained from Ebira comm... more Plasmid curing of microbes and physicochemical analysis of water samples obtained from Ebira communities in six local governments in Ekiti South Senatorial District were analyzed. Antibiotic sensitivity and profile of bacterial isolates were analyzed using pour plating, disk diffusion method and gel electrophoresis techniques respectively while the plasmid were cured using acridine orange. The mean total bacterial count of the water samples collected from these six different local governments at different time ranged from 2.08 x 10 5 to 6.0 x 10 6 CFU/ml; the mean total coliform count ranged from 2.41 x 10 5 to 3.75 x 10 6 CFU/ml and the mean total Escherichia coli count (TEC) ranged from 1.53 x 10 5 to 3.45 x 10 5 CFU/ml. Total of 152 bacteria were recovered with E.coli having the highest distribution of 35% while Serratia marcensens had the least distribution of 0.7%. The highest antibiotic resistance of 100% was recorded against ceftazidine but only 17% of the isolates were resistant to gentamicin. About 56% of 34 selected MAR isolates carried plasmid(s) with high molecular weight ranging from 5.64Kbp to 23.13Kbp. Antibiotic resistance pattern and plasmids profile of selected MAR E.coli, Pseudomonas aeruginosa and Staphylococcus aureus prior to and after curing showed that Pseudomonas aeruginosa became susceptible to augmentin and Staphylococcus aureus also became susceptible to ceftriazole while E. coli still maintained the earlier resistant pattern. The plasmid profiling of these isolates after curing indicated the lost of plasmids in each of the isolates. Present study however implicated the incidence of MAR bacteria in the sources of water in Ekiti-South Senatorial district as a serious health challenge, and confirmed the potential of acridine orange for plasmid curing.
CRC Press eBooks, Jan 16, 2024
OA Molecular and Cell Biology, 2013
Therapeutic Proteins Against Human Diseases, 2022
Synbiotics for the Management of Cancer, 2023
Journal of Advances in Biology & Biotechnology, Nov 17, 2022
Klebsiella species are one of the major causes of systematic infections, theses gram negative org... more Klebsiella species are one of the major causes of systematic infections, theses gram negative organisms are also capable of housing various resistant genes to resist the potency of conventional antibiotics. These observations account for the need to assess the antibacterial activity of Gentamicin and natural products against Klebsiella. A total of fifty (50) clinical isolates of Klebsiella species of different pathological sources were collected from four different Teaching Hospitals in Southwest, Nigeria. The identification of all the isolates was done using conventional biochemical tests. Antibiogram was carried out on all 50 Klebsiella clinical isolates using multiple antibiotic discs and the sensitivity of honey was done using the agar diffusion method. In the antimicrobial susceptibility test on honey, two undiluted different samples of honey (Honey A Refined and Honey B Natural) showed high activity and 1:2 to 1:6 aq. dilutions showed less activity against the Klebsiella isolates. Gentamicin used at the concentration of 4.0µg/ml has great activity against the isolates but was basically lower than the antibacterial activity of each undiluted honey. In the occasion of therapeutic disaster with gentamicin or any other associated antibiotics, honey offers an appropriate and improved alternative in dealing with infected burn wounds and other infections like urinary tract infections, nosocomial infections, etc.
Therapeutic Proteins Against Human Diseases, 2022
Handbook of Animal Models and its Uses in Cancer Research, 2022
Microbiome in Inflammatory Lung Diseases, 2022
Plasmid curing of microbes and physicochemical analysis of water samples obtained from Ebira comm... more Plasmid curing of microbes and physicochemical analysis of water samples obtained from Ebira communities in six local governments in Ekiti South Senatorial District were analyzed. Antibiotic sensitivity and profile of bacterial isolates were analyzed using pour plating, disk diffusion method and gel electrophoresis techniques respectively while the plasmid were cured using acridine orange. The mean total bacterial count of the water samples collected from these six different local governments at different time ranged from 2.08 x 10 5 to 6.0 x 10 6 CFU/ml; the mean total coliform count ranged from 2.41 x 10 5 to 3.75 x 10 6 CFU/ml and the mean total Escherichia coli count (TEC) ranged from 1.53 x 10 5 to 3.45 x 10 5 CFU/ml. Total of 152 bacteria were recovered with E.coli having the highest distribution of 35% while Serratia marcensens had the least distribution of 0.7%. The highest antibiotic resistance of 100% was recorded against ceftazidine but only 17% of the isolates were resistant to gentamicin. About 56% of 34 selected MAR isolates carried plasmid(s) with high molecular weight ranging from 5.64Kbp to 23.13Kbp. Antibiotic resistance pattern and plasmids profile of selected MAR E.coli, Pseudomonas aeruginosa and Staphylococcus aureus prior to and after curing showed that Pseudomonas aeruginosa became susceptible to augmentin and Staphylococcus aureus also became susceptible to ceftriazole while E. coli still maintained the earlier resistant pattern. The plasmid profiling of these isolates after curing indicated the lost of plasmids in each of the isolates. Present study however implicated the incidence of MAR bacteria in the sources of water in Ekiti-South Senatorial district as a serious health challenge, and confirmed the potential of acridine orange for plasmid curing.