Maretaningtias Dwi Ariani | Universitas Airlangga (original) (raw)

Papers by Maretaningtias Dwi Ariani

Legal Medicine, 2022

Age estimation of unidentified bodies is of marked importance in forensic medicine. In previous s... more Age estimation of unidentified bodies is of marked importance in forensic medicine. In previous studies, the analysis of DNA methylation in body fluids led to the identification of several age-related CpG sites in genes such as EDARADD and FHL2. However, limited information is available on whether interethnic differences may affect the age prediction results. In the present study, we examined the effect of ethnicity on the age prediction method based on methylation scores, which were determined via methylation-sensitive high-resolution melting. We found that there was a significant difference in methylation scores between Japanese and Indonesian participants of early 20 s group, and that the nationality coefficient was significant for age estimation when applying the existing method for the analysis of the methylation status of EDARADD and FHL2. This suggests that when using certain biochemical indicators as a predictor of age, the effects of ethnicity on DNA methylation should be considered to improve the accuracy of the estimation.

Proceedings of the 7th International Meeting and the 4th Joint Scientific Meeting in Dentistry, 2017

Background: Scaffold is a medium for extracellular matrix synthesis and Becomes a place for attac... more Background: Scaffold is a medium for extracellular matrix synthesis and Becomes a place for attachment and growth of new cells. Some polymeric materials, the which have been developed in tissue engineering are chitosan. Chitosan is one of the best materials used in tissue engineering due to its biodegradable, biocompability, anti-bacterial properties, for wound healing and bioadded characters. The carbonate apatite material is used as a biocompatible material for medical purposes. Carbonate apatite Increased material the chemical bonds between biomaterials and bone tissue that can Affect bone formation. The nanostructured biomaterials Significantly Enhance cell functioning leading to Increased osteoinductivity and oseointegrativity. So based on the explanation and understanding of the mechanisms of the relationship between the bone regeneration process and the nano chitosancarbonate apatite scaffold, it is important to improve the method for the next generation of scaffolds that can demonstrate bone efficiency and regeneration in vitro. Objective: To Determine the Increased expression of FGF-2 on osteoblast cell planting in nano chitosancarbonate apatite scaffold and the results of this study are expected to be used as a selection method of bone graft using nano-chitosan scaffold. Methods: Laboratory experimental study with post test only group design on seven samples, the osteoblast cells planted on chitosan nano-carbonate apatite scaffold with observation time on day 3, day 5, and day 7 and Increased expression of FGF-2 osteoblast cells were measured by Immunohistochemistry (IHC) method. The results were Analyzed by statistical test of multiple comparison test of Post Hoc and Tukey test. Result: The results Showed a significant difference (P <0.05) between treatment groups in the observation time on day 3, 5 and 7. Conclusion: The experiment has shown that the increase is of FGF-2 expression in osteoblast cells planted in chitosan nanocarbonate apatite scaffold has Increased in observation time on day 3, 5 and 7.

Dentika: Dental Journal, 2014

Untuk meningkatkan adhesi sel dan kemampuan pembentukan tulang dari kitosan, dicoba untuk membuat... more Untuk meningkatkan adhesi sel dan kemampuan pembentukan tulang dari kitosan, dicoba untuk membuat suatuscaffolds yang menggabungkan kitosan dengan carbonate apatite (CA). Tujuan penelitian ini adalah untukmembuat carbonate apatite-chitosan scaffolds (CA-ChSs) serta mengevaluasi CA-ChSs dari sudut pandangproliferasi sel menggunakan MC3T3-E1. Chitosan scaffolds (ChSs) yang berisi 25, 50, 75, 100, 125, 150, 200dan 400 mg bubuk kitosan (100D, YSK, Japan) dibuat dengan prosedur dilarutkan dalam 5 ml asam asetat 2%,dikocok selama 15 menit, kemudian dinetralkan dengan 15 ml 0,1 M larutan NaOH. Setelah disentrifugasi pada1500 rpm selama 10 menit, kelebihan air dibuang, kemudian gel kitosan dikemas ke dalam cetakan teflon(diameter: 5 mm, tinggi: 2 mm). Cetakan dibekukan pada suhu -80°C selama 2 jam dan dikeringkan dalammesin beku kering pada suhu -54°C selama 24 jam. Selanjutnya dilakukan radiasi ultraviolet pada ChSs selama 2jam. Untuk membuat CA-ChSs, dipilih ChSs yang berisi 200 mg bubuk ...

Dental Journal (Majalah Kedokteran Gigi), 2017

Background: Over 500.000 of the 2,3 million surgical treatments requiring bone grafting procedure... more Background: Over 500.000 of the 2,3 million surgical treatments requiring bone grafting procedures that are performed annually are likely to be necessitated by or will result in bone defects that will not regenerate. Treatment to regenerate new tissues is needed, especially for hard tissue repair, which not only relies on a natural osseointegration process, but also requires a physical support to guide the differentiation and proliferation of cells into the targeted functional tissue. Chitosan and collagen extracted from chicken feet combinations are expected to enhance the bioactive surface and provide mechanical strength as a bone graft scaffold. Purpose: The aim of this study was to investigate the role of chitosan and collagen scaffold synthesized from chicken feet applications to increase the expression of Osteoprotegerin (OPG) and osteoblast cells on the fourteenth day of bone healing. Methods: Eighteen three-month old, adult, male, Rattus novergicu strain rodents with a body ...

Latar belakang: Resorpsi tulang alveolar setelah pencabutan gigi merupakan suatu proses fisiologi... more Latar belakang: Resorpsi tulang alveolar setelah pencabutan gigi merupakan suatu proses fisiologis yang tidak dapat dihindari. Kombinasi ekstrak kulit buah manggis dengan DFDBBX diharapkan dapat memberikan sifat osteokonduktifitas dan kekuatan graft yang lebih memuaskan untuk membantu menstimulasi pembentukan tulang baru. Tujuan: Mengevaluasi efek klinis dan mikrobiologis pemberian kombinasi ekstrak kulit buah manggis dan DFDBBX terhadap ekspresi FGF-2, jumlah osteoblas dan osteoklas pada tulang alveolar setelah pencabutan gigi Cavia cobaya. Metode: Cavia cobaya sebanyak 56 ekor dibagi menjadi 8 kelompok, setiap kelompok terdiri dari 7 ekor. Kelompok I & II diisi 24 gram PEG pada soket giginya. Kelompok III & IV diisi 0.5 gram DFDBBX dan 24 gram PEG pada soket giginya. Kelompok V & VI diisi 0.5 gram ekstrak kulit buah manggis dan 24 gram PEG pada soket giginya. Kelompok VII & VIII diisi dengan kombinasi 0.5 gram DFDBBX, 0.5 gram ekstrak kulit buah manggis dan 24 gram PEG pada soket ...

Background: Tooth loss is a common case in dental clinics. Tooth loss can be replaced with fixed ... more Background: Tooth loss is a common case in dental clinics. Tooth loss can be replaced with fixed dentures, removable partial dentures (RPDs), or implants. However, a dental treatment with RPDs is more attractive to public than fixed dentures and implants. RPDs can generally be classified into four types, namely class I, class II, class III, and class IV. Nevertheless, RPDs classified into Kennedy class I and class II have more problems than RPDs class III and class IV. Objective: This study is aimed to determine the satisfaction of users of acrylic removable partial dentures classified as Kennedy class I and II in Prosthodontics clinic of RSGMP, Universitas Airlangga. Methods: Respondents of the study were thirty-eight patients who had been treated at Prosthodontics clinic of RSGMP, Universitas Airlangga in January 2013 - September 2014. Those patients then were interviewed and asked to fill questionnaires related to the satisfaction of RPDs users. Results: Based on the result data,...

Stem cells can give a new hope to accelerate wound healing process and can also be used for the t... more Stem cells can give a new hope to accelerate wound healing process and can also be used for the treatment of various diseases, including diseases on periodontal tissues (periodontitis) since there is still not any materials known to be able to attach wobbly teeth perfectly. Adult stem cells derived from bone marrow have widely been used nowadays. However, the number of stem cells is limited, consequently, growth factors are necessary to increase the proliferation of stem cells. Unfortunately, growth factors that have been used are still expensive and difficult to obtain. Therefore, an alternative to the use of growth factors from natural materials that can potentially accelerate wound healing process is necessary to be developed. Purpose: Golden Sea Cucumbers (Stichopus hermanii) is rich in both growth factors that can repair damaged cells and protein that reaches up to 82% of all components of the golden sea cucumbers, 80% of which is collagen. Therefore, this research aims to prov...

Dental Journal: Majalah Kedokteran Gigi, 2012

Background: The natural biopolymer chitosan (Ch) is currently regarded as a candidate for bone ti... more Background: The natural biopolymer chitosan (Ch) is currently regarded as a candidate for bone tissue engineering. However, Ch is poor for cell adhesion and low bone formation ability. In order to enhance cell adhesion and bone formation ability, combination of Ch with carbonate apatite (CA) was developed. Purpose: The aim of this study was to make carbonate apatite-chitosan scaffolds (CAChSs) and evaluate its osteoconductivity in terms of cell proliferation. Methods: Chitosan scaffolds (ChSs) were made by the following procedure. Twenty-five, 50, 100, 200 and 400 mg Ch was dissolved into 5 ml of 2% acetic acid (CH 3 COOH), shaked for 15 min and neutralized with 15 ml of 0.1 M sodium hydroxide (NaOH) solution. After centrifugation, Ch gel was packed into the molds then frozen at -80°C for 2h and dried in a freeze dry machine for 24h. The sponges were subjected to UV radiation for 2h. To make CA-ChSs, 200 mg Ch was selected. After neutralization, 50 mg of 0.06 M CA were added into th...

European Journal of Dentistry, 2020

Objective This study aims to combine natural propolis with bovine bone graft (BBG) as a means of ... more Objective This study aims to combine natural propolis with bovine bone graft (BBG) as a means of extraction socket preservation after 3 and 7 days toward expression of heat shock protein (HSP) 70 and osteocalcin to regenerate bone. Materials and Methods The Cavia cobaya were divided into eight groups, each consisting of seven samples. Their lower left incisors were extracted and induced with PEG, propolis extract, BBG, and a combination of propolis extract BBG. The research subjects were terminated on days 3 and 7 postextraction. Immunohistochemical and histopathological examinations were subsequently performed to observe HSP 70 expression, osteocalcin expression, osteoblasts, and osteoclasts. Statistical Analysis Data obtained were then analyzed with one-way analysis of variance (ANOVA) and Tukey’s honestly significant difference (HSD) tests. Results Both the groups with the combination of propolis extract and BBG on days 3 and 7 were found to present the highest number of HSP70 ex...

Cumhuriyet Dental Journal, 2019

Objectives: Chitosan is a popular bone graft material. However, chitosan also has a weakness for ... more Objectives: Chitosan is a popular bone graft material. However, chitosan also has a weakness for cell adhesion and the lack of sufficient bone formation capabilities. To improve it, we tried to develop a chitosan scaffolds that combine with carbonate apatite (CA), which has excellent biocompatibility properties of the tissues of the human body. We tried to find the most appropriate amount of Carbon Apatite (CA) to be combined with Chitosan scaffold (ChSs) to produce a good scaffolds structure, as well as to evaluate CA-ChSs from the standpoint of cell proliferation using mesenchymal stem cells (MSCs). Materials and Methods: Porous chitosan matrix was made by using the lyophilization technique. ChSs containing chitosan powder was made by the following procedure. 100, 150, 200, and 400 mg of chitosan powder was dissolved in 5 ml of 2% acetic acid at room temperature. ChSs containing 200 mg of chitosan powder was chosen to make CA-ChSs. Ultraviolet radiation was then performed for 2 hours. CA-ChSs structure was observed by a scanning electron microscope (SEM). Proliferation of MSCs in CA-ChSs evaluated at days 1, 3, 5 and 7. Results: This study demonstrated that CA-ChSs containing 200 mg of chitosan powder and 50 mg CA has a three-dimensional structure that is porous and attachment powder CA in the pores and absorbance values were increased from the examination day 1 until day 7. Cell proliferation using MSCs in CA-ChSs are better, as the absorbance value of CA-Chss with 50 mg CA content was significantly higher than ChSs. These findings also confirm that MSCs has good viability and biocompatibility in bone tissue engineering techniques. Conclusions: Based on these results, it is expected CA-ChSs may be candidates for bone graft material in tissue engineering techniques.

Clinical, Cosmetic and Investigational Dentistry, 2019

The aim of this study is to asses the relationship between the level of sIgA and dental caries ex... more The aim of this study is to asses the relationship between the level of sIgA and dental caries experience in healthy children who are 6-to 9-years-old from Indonesia. The case-control study is conducted to determine the protective role of salivary secretory immunoglobulin A (sIgA) levels in the stimulated whole saliva of dental caries-active and caries-free children. Methods: This research was done by stimulating the whole saliva which had been collected from 6-to 9-years-old children with the index def-t≥3 of 30 children as the caries-active children group and 30 children with def-t<3 as the low caries-active children group. Saliva samples were collected in sterile vials between 10 am-12 pm due to the circadian rhythm, which is at least one hour after last meal. 1,5 ml of collected salivary sample was centrifuged, then the supernatants was transferred to other tube and stored immediately to the laboratory at a temperature of-20°C. The estimation of sIgA concentration was done by using ELISA. The differences in the level of sIgA between the two groups with caries were analyzed using the t-test afterward. Results: The total salivary concentration of sIgA was statistically significantly higher in the low caries-active children group than in the caries-active children group. Conclusion: The total salivary concentration of sIgA was statistically and significantly higher in the low caries-active children group than caries-active children Group. There is a negative correlation between sIgA level and dental caries activity of 6 to 9-years-old children.

Dental Research Journal, 2019

Background: Low levels of estrogen can cause osteoporosis and usually occur during a woman's meno... more Background: Low levels of estrogen can cause osteoporosis and usually occur during a woman's menopausal phase. Osteoporosis can lead to bone resorption, the absence of osseointegration, and implant failure. The aim of this study is to determine the expression of transforming growth factor-beta 1 (TGF-β1), runt-related transcription factor (RUNX2), and osteoblasts in mandibular rats with low levels of estrogen. Materials and Methods: This study is an in vivo experimental research. Female Wistar rats (n = 18) were divided into two groups: (1) Postsham surgery and (2) ovariectomy group. After 12 weeks, the rats were sacrificed to identify the level of estrogen, while histological analysis was conducted to determine the level of osteoblast and the expression of TGF-β1 and RUNX2. The data were analyzed using t-test (P < 0.05). Results: There were significant lower levels of estrogen and osteoblast among the ovariectomy group compared to the postsham group (P < 0.05). RUNX2 levels were found to be significantly higher in the ovariectomy group than that in the postsham group (P < 0.05). However, there were no significant differences between TGF-β1 levels within the ovariectomy and postsham groups (P > 0.05). Conclusion: Ovariectomy can lead to decreased osteoblastogenesis in mandibular bone by the reduced level of osteoblast and the increased expression of TGF-β1 and RUNX2.

The Journal of Indian Prosthodontic Society, 2017

Background: Following the extraction of a tooth, bone resorption can cause significant problems f... more Background: Following the extraction of a tooth, bone resorption can cause significant problems for a subsequent denture implant and restorative dentistry. Thus, the tooth extraction socket needs to be maintained to reduce the chance of any alveolar ridge bone resorption. Objective: The objective of this study is to determine whether the administration of mangosteen peel extracts (MPEs), combined with demineralized freeze-dried bovine bone xenograft (DFBBX) materials for tooth extraction socket preservation, could potentially reduce inflammation by decreased the expression of nuclear factor κβ (NfKb) and receptor activator of nuclear factor-κβ ligand (RANKL), to inhibit alveolar bone resorption, and increased of bone morphogenetic protein-2 (BMP2) expressions to accelerate alveolar bone regeneration. Materials and Methods: This study consists of several stages. First, a dosage of MPE combined with graft materials was applied to a preserved tooth extraction socket of a Cavia cobaya. Second, the C. cobaya was examined using immune histochemical expression of NfKb, RANKL, BMP2, as well as histology of osteoblasts and osteoclasts. The research was statistically analyzed, using an analysis of variance test and Tukey honest significant difference test. Results: The results of this research were that it was determined that MPEs combined with graft materials on a preserved tooth extraction socket can reduce NfKb, RANK, and osteoclasts also increase of BMP2 and osteoblast. Conclusion: The induction of MPEs and DFBBX is effective in reducing inflammation, lowering osteoclasts, decreasing alveolar bone resorption, and also increasing BMP2 expression and alveolar bone regeneration.

The Journal of Indian Prosthodontic Society, 2015

Tissue engineering is required as an emerging technology to reconstruct damaged bone because of t... more Tissue engineering is required as an emerging technology to reconstruct damaged bone because of tumors, trauma or pathologic disease. The bone formation using bone graft material is a widely accepted technique in the surgical field. In order to repair damaged bone, autogenous bone has been the ideal choice to fill the defect site. However, autografts sometimes have significant limitations coming from a donor site morbidity, a limited donor bone supply, and an inadequate resorption rate during the healing process. [1,2] These limitations have prompted Background: Collagen is regarded as one of the most useful biomaterials. We tried to combine collagen and carbonate apatite (CA) with some cytokines in order to enhance bone formation ability. In this study, we found that CA-collagen sponge (CA-CS) was a possible candidate of newly graft material for bone formation. Materials and Methods: CA-CS was fabricated by the following procedure. One wt% of pig hide collagen solution (Nippon Meat Packers. Inc., Tokyo, Japan) was neutralized with 0.1 N NaOH, and then mixed immediately 243 mg apatite powder with 0.06 M carbonate contents. After centrifugation at 1500 rpm for 10 min, excess water was removed, and the mixture was packed into Teflon molds (5.0 mm × 2.0 mm). Each 10 µg of basic fibroblast growth factor (bFGF) and recombinant human bone morphogenetic protein-2 (rh-BMP2) were involved in these sponges. Then these scaffolds frozen at −80°C for 2 h and dried in a freeze dry machine for 24 h. CA-CS without cytokines were also prepared as a control. Mouse osteoblast-like cell (MC3T3-E1) proliferations in these scaffolds were investigated by 3-day in vitro cell culture using MTT assay examination. Ten New Zealand rabbits (weight: 3-3.5 kg) were used in this in vivo study. After 3 weeks of placement, the scaffolds, rabbits were sacrificed, and bone formation in the sockets was evaluated histologically and histomorphometrically. Results and Conclusion: By histological observation and measurement of bone area ratio, CA-CS with cytokines showed higher bone formation ability (bFGF/CA-CS: 50.7 ± 7.3%, rh-BMP2/CA-CS: 54.2 ± 5.0%) than other groups. From the limited results of this study, it is suggested that CA collagen scaffolds with some cytokines may become an attractive scaffold for bone regeneration.

Dental Journal (Majalah Kedokteran Gigi), 2009

Background: A biomaterial used in oral cavity should not become toxic, irritant, carcinogenic, an... more Background: A biomaterial used in oral cavity should not become toxic, irritant, carcinogenic, and allergenic. Chitosan represents a new biomaterial in dentistry. Purpose: To examine the toxicity of chitosan from tiger prawn shell waste on cell culture with MTT assay. Methods: Chitosan with concentration of 0.25%, 0.5%, 0.75% and 1% was used in this experiment. Each sample was immersed on eppendorf microtubes containing media culture. After 24 hours, the immersion of media culture was used to examine the toxicity effects on BHK-21 cell based on MTT assay method. The density of optic formazan indicates the number of living cells. All data were then statistically analyzed by one-way Anava. results: The number of living cells in chitosan from tiger prawn shell waste was 93.16%; 85.07%; 78.48%; 75.66%. Thus, there was no significant difference among groups. Conclusion: Chitosan with 0.25%, 0.5%, 0.75% and 1% concentrations from tiger prawn shell waste were not toxic for BHK-21 cell culture when using parameter CD 50 .

Dental Journal (Majalah Kedokteran Gigi), 2015

Background: Tissue engineering has given satisfactory results as biological tissue substitutes to... more Background: Tissue engineering has given satisfactory results as biological tissue substitutes to restore, replace, or regenerate tissues that have a defect. Chitosan is an organic biomaterial often used in the biomedical field. Chitosan has biocompatible, antifungal, and antibacterial properties. Chitosan is osteoconductive, suitable for bone regeneration applications. Bone defect healing begins with inflammatory phase as a response to the presence of vascular injury, so new vascularization is required. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor-2 (FGF2) are indicators of the beginning of bone regeneration process, playing an important role in angiogenesis. Purpose: This research was aimed to determine the effects of chitosan scaffold application on the expressions of VEGF and FGF2 in tissue engineering principles. Method: Chitosan was dissolved in CH 3 COOH and NaOH to form a gel. Chitosan gel was then printed in mould to freeze dry for 24 hours. Those rats with defected bones were divided into two groups. Group 1 was the control group which defected bones were not administrated with chitosan scaffolds. Group 2 was the treatment group which defected bones were administrated with chitosan scaffolds. Those rats were sacrificed on day 14. Tissue preparations were made, and then immunohistochemical staining was conducted. Finally, a statistical analysis was conducted using Kruskal Wallis test. Result: There was no significant difference in the expressions of VEGF and FGF2 between the control group and the treatment group (p>0.05). Conclusion: Chitosan scaffolds do not affect the expressions of VEGF and FGF2 during bone regeneration process on day 14 in tissue engineering principles

Dental Materials Journal, 2013

Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique... more Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique. It was found that ChSs prepared with 200 mg chitosan powder (ChSs200) had well-structured three-dimensional architecture with high porosity and good retentive form without brittleness. In addition, it was shown that the number of osteoblast-like cells MC3T3-E1 proliferated on desalinated ChSs200 was larger than that on the non-desalinated ChSs200. CA-ChSs were fabricated by adding 100 mg carbonate apatite (CA) to 200 mg chitosan gels followed by freeze-drying (CA100ChSs200). SEM observation revealed that CA100ChSs200 had favorable threedimensional porous structures. The number of living cells increased more rapidly on CA100ChSs200 prepared with different amounts of CA than on ChSs. ALP activity significantly increased after day 14 and reached a plateau after day 21 in ChSs200 and CA100ChSs200. It was concluded that newly developed CA100ChSs200 may be a possible scaffold material for tissue engineering.