Puspa Wardhani | Universitas Airlangga (original) (raw)

Papers by Puspa Wardhani

Research paper thumbnail of Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia

Journal of Tropical Biodiversity and Biotechnology

An urban park is potentially a source of vector-borne disease transmission due to it being a natu... more An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F15...

Research paper thumbnail of Profil Virus Dengue DI Surabaya Tahun 2008–2009

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Four serotypes of dengue viruses (DENV) 1–4 are mosquito-borne human pathogens that cause widespr... more Four serotypes of dengue viruses (DENV) 1–4 are mosquito-borne human pathogens that cause widespread epidemics withconsiderable morbidity and mortality. The aim of this study was to evaluate the dengue serotypes profile, which were circulating inSurabaya. This research has been carried out consisting of 360 samples from patients with dengue virus infections, according the WorldHealth Organization (WHO) criteria. These sera were collected from patients Dr. Soetomo Hospital and private laboratory in Surabayafrom 2008–2009. From 360 samples, 68 samples (18.9%) were undifferentiated fever, 53 samples (14.7%) were dengue fever, 239samples (66.4%) were dengue hemorrhagic fever. From 58 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) samples, 25samples (43%) were positive, consisting of 52% DEN-2, 20% DEN-1, 16% DEN-3 and 12% DEN-4. These results showed that fourserotypes are circulating in Indonesia, dominated by DEN-2, followed by DEN-1, DEN-3 and DEN-4.

Research paper thumbnail of Inhibitory Activity of Cobalt(II)–Morin Complex Against the Replication of Dengue Virus Type 2

Indonesian Journal of Tropical and Infectious Disease

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease... more Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. Antidengue treatments are urgently required to control the emergence of dengue. DENV is a mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in the tropical and sub-tropical areas and transmitted to humans by Aedes agypty and Aedes albopictus. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is an important alternative for the characterization and development of drugs. Metal–organic compounds were reported to exhibit fungicidal, bactericidal, and antiviral activities its inhibitory activity was not significant, at high concentration it was more toxic to replicating cells than to stationary cell monolayers of Vero cells. The aim of this study is to investigate the antiviral effects of Cobalt(II)–Morin com...

Research paper thumbnail of Suppressive effect of goat bile in Plasmodium berghei ANKA infection in mice

Veterinary World

Background and Aim: Some individuals in Indonesia consume intact goat gallbladder to prevent and ... more Background and Aim: Some individuals in Indonesia consume intact goat gallbladder to prevent and treat malaria. The acute and subacute toxicity tests of goat bile (GB) have shown mild diarrhea in mice. Therefore, this study aimed to evaluate the suppressive effect of GB on parasitemia, splenomegaly, hepatomegaly, and blood biochemistry to assess liver and kidney function in BALB/c mice infected with Plasmodium berghei ANKA. Materials and Methods: Fifty healthy mice were infected with P. berghei ANKA and divided into five groups. Mice in three groups were administered 0.5 mL of 25%, 50%, or 100% of GB by gavage. Animals in Group 4 were administered 187.2 mg/kg BW of dihydroartemisinin-piperaquine phosphate as a positive control (POS Group). Mice in fifth group were administered sterile water as negative (NEG) controls. Further, 30 uninfected mice were divided into groups 6-8 and administered GB as were mice in the first three groups. Group 9 included 10 uninfected and untreated anima...

Research paper thumbnail of The Association of Blood Sugar Nuchter and Total Cholesterol Serum Levels with Platelet Hyperaggregation in Patients with Peripheral Diabetic Neuropathy

Research paper thumbnail of Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF)

Majalah Obstetri & Ginekologi

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer... more Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia.

Research paper thumbnail of Immature granulocyte and mean platelet volume as a predictor of 30-day postoperative mortality in patients with sepsis caused by peritonitis

Bali Journal of Anesthesiology

Research paper thumbnail of Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

Indonesian Journal of Tropical and Infectious Disease

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease... more Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many efforts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitor...

Research paper thumbnail of Correlation of Neutrophils/Lymphoctes Ratio and C-Reactive Protein in Sepsis Patients

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Sepsis merupakan penyebab umum dari kesakitan dan kematian di seluruh dunia. Diagnosis yang cepat... more Sepsis merupakan penyebab umum dari kesakitan dan kematian di seluruh dunia. Diagnosis yang cepat dan tepat sangatdiperlukan. Salah satu respons fisiologis pada sistem imunitas terhadap inflamasi sistemik adalah peningkatan jumlah neutrofil danpenurunan jumlah limfosit. Rasio neutrofil/limfosit telah diketahui sebagai petanda inflamasi yang cepat, sederhana dan murah. Tujuanpenelitian ini adalah membuktikan adanya kenasaban antara rasio neutrofil/limfosit dan c-reactive protein di pasien sepsis. Penelitianbersifat ptong lintang observasional, dilakukan masa waktu Januari hingga Juni 2015. Sampel penelitian terdiri dari 42 pasien sepsisyang dirawat di Instalasi Rawat Darurat RSUD Dr. Soetomo Surabaya. Semua sampel dilakukan pemeriksaan jumlah limfosit, neutrofil,CRP. Hasil dianalisis menggunakan uji kenasaban Spearman’s. Rasio neutrofil/limfosit berkisar antara 3,42–57,47 (21,74±14,1). KadarCRP antara 1,22 mg/L–361,86 mg/L (158 mg/L±97,4 mg/L). Hasil penelitian tidak terdapat kenasab...

Research paper thumbnail of Comparison Results of Analytical Profile Index and Disc Diffusion Antimicrobial Susceptibility Test to Technical Dedicated Reasonable 300B Method

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Angka kematian infeksi aliran darah cukup tinggi, berkisar 20–50%. Patogen penyebab dapat dibukti... more Angka kematian infeksi aliran darah cukup tinggi, berkisar 20–50%. Patogen penyebab dapat dibuktikan dengan pemeriksaan kulturdarah yang dilanjutkan dengan uji kepekaan antibiotika. Metode pemeriksaan dapat dilakukan secara manual atau automatis baiksemiautomatis ataupun automatis penuh. Metode manual relatif tidak memerlukan biaya yang besar dibandingkan metode automatisasi.Penelitian ini merupakan analisis observasional dengan desain potong lintang. Metode identifikasi manual memakai metode API danuji kepekaan antibiotika metode difusi cakram antibiotika Kirby Bauer. Kedua metode ini dibandingkan dengan metode semiautomatisTDR-300B. Metode automatis penuh VITEK 2 digunakan sebagai metode rujukan untuk menilai kinerja metode konvensional dansemiautomatis. Bakteri penyebab infeksi aliran darah didominasi Gram negatif kebanyakan Eschericia coli dan Klebsiella pneumonia.Ketepatan metode identifikasi API terhadap VITEK 2 sebesar 87,87%, ketepatan identifikasi metode TDR-300B terhadap m...

Research paper thumbnail of Kemampuan Uji Tabung Widal Menggunakan Antigen Import Dan Antigen Lokal

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Despite of its higher specificity than Widal-slide test, Widal-tube test is not widely used by me... more Despite of its higher specificity than Widal-slide test, Widal-tube test is not widely used by medical laboratories because it is not practical and each laboratory has to produce their own antigens. The Laboratory must have sufficient microbiology equipments and reagents to produce antigens. REMEL® provides ‘ready for use’ antigens to perform Widal-tube test. To Compare and correlate Widal-tube test using REMEL® imported antigens and local antigens (produced by lab. Clinical pathology Dr. Soetomo hospital). The samples were tested by Widal-tube test, using REMEL® and local antigens, comparing Salmonella typhi (ST) O antigen, ST H antigen, S. paratyphi (SP) A H antigen, and SP B H antigen for each method, with twofold dilution from 1/40 until 1/1280. The number of samples was 55. The results are defined as pathologic (above cut-off value) and non pathologic (below cut-off value). REMEL® ST O antigen had a significant correlation to local antigens (r = 0.665, p < 0.01). REMEL® ST H...

Research paper thumbnail of AST, ALT and Albumin Level in Chronic Hepatitis B Patients with and without Complications of Cirrhosis and Hepatocellular Carcinoma

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carci... more Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carcinoma (HCC). Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and albumin may be used as indicators of hepatocyte damage. This study aimed to determine differences between AST, ALT, and albumin in CHB patients without complications with CHB patients with cirrhosis and HCC complications. An analytical cross-sectional study was conducted in March-May 2019 on 62 CHB patients with or without cirrhosis or HCC complications using the total sampling method. AST and ALT were calculated using the Siemens Dimension device and IFCC method. The data were analyzed using independent samples T-test Albumin in CHB patients without complications was higher than CHB patients who had complications of liver cirrhosis (p=0.002). The AST and ALT were not significantly different. Aspartate aminotransferase in CHB with cirrhosis complications differed from CHB patients who had HCC complications ...

Research paper thumbnail of Dengue Virus Serotype 4 Is Responsible for the Outbreak of Dengue in East Java City of Jember, Indonesia

Viruses

Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2;... more Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was the least-reported serotype in Indonesia during the last five decades. We recently conducted a molecular epidemiology study of dengue in the Jember regency, East Java province, Indonesia. Dengue is endemic in the region and outbreaks occur annually. We investigated the clinical characteristics and etiology of dengue-like febrile illness in this regency to understand the disease dynamics. A total of 191 patients with clinical symptoms similar to dengue were recruited during an 11-month study in 2019–2020. Children accounted for the majority of cases and dengue burden was estimated in 41.4% of the cases based on NS1 antigen, viral RNA, and IgG/IgM antibody detection with the majority (73.4%) being primary infections. Secondary infection was significantly associated with a higher risk of severe dengue manifestation. All four DENV serotypes were detected in Jember. Str...

Research paper thumbnail of Pengaruh Rendaman Air Terhadap Kualitas DNA pada Sperma dengan STR-CODIS D13S317 dan D21S1

Jurnal Biosains Pascasarjana

AbstrakKriminalitas yang terjadi akibat kejahatan seksual banyak terjadi di mana-mana. Kejahatan ... more AbstrakKriminalitas yang terjadi akibat kejahatan seksual banyak terjadi di mana-mana. Kejahatan ini bisa menimpa beberapa orang dan dari berbagai umur. Dalam kasus ini pasti akan di temukan barang bukti di tempat kejadian perkara. Salah satu bukti akibat kejahatanseksual adalah bercak sperma. Bercak sperma dapat di temukan seperti pada pakaian yang digunakan korban atau pelaku. Dengan keterbatasan waktu yang dimiliki oleh polisi tentunyaperlu cara untuk pengidentifikasi siapa pelaku dari kejahatan tersebut. Sampel bercak sperma akan di isolasi DNA. Isolasi DNA adalah memisahkan DNA yang ada pada sel sperma dengan komponen yang lainnya. Setelah DNA di dapatkan akan di ketahui kadar dankemurniannya. Hasil tersebut bisa di pengaruhi oleh media bercak sperma berada. Faktor- faktor tersebut salah satunya media air dan kain katun. Sifat kain katun adalah mempunyaikemampuan menyerap yang tinggi walaupun dalam keadaan basah sekalipun. Setelah isolasi DNA, tahapan selanjutnya ...

Research paper thumbnail of The incidence of Clostridium Difficile infection in diarrhea patients after receiving antibiotics at Dr. Soetomo Hospital Surabaya

Research paper thumbnail of The Correlation between leucocyte CD64, Immature Granulocyte and Presepsin with Procalcitonin in Bacterial Sepsis Patient

Research paper thumbnail of Acute and subacute toxicity tests of goat bile in BALB/c mice

Veterinary World

Aim: The aim of this study was to investigate the toxicity of goat bile in BALB/c mice since some... more Aim: The aim of this study was to investigate the toxicity of goat bile in BALB/c mice since some Indonesian people consume raw goat gallbladder to treat malaria and increase stamina. Materials and Methods: Acute toxicity test was done in six groups of BALB/c mice using 100%, 50%, 25%, 12.5%, and 6.75% of goat bile and negative control. The death of mice was observed within 14 days. In the subacute toxicity test, the body weight and hematology parameters on day 0 and day 4 post-treatment were evaluated. The mice were closely observed for 28 days before plasma collection for the blood biochemistry evaluation. Results: Mild diarrhea was observed in acute and subacute toxicity tests. No death of mice was observed in acute test. Goat bile did not inhibit the increase of the body weight of mice. A slight reduction in hemoglobin and hematocrit levels in mice treated with 25% and 50% goat bile, however, remained normal in mice treated with 100% goat bile. The red and white blood cell count...

Research paper thumbnail of Contamination of water and soil of rice fields with soil transmitted helminths as source of transmission to farmers in Grogol sub-district, Kediri district

Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya

ABSTRACTSoil-transmitted helminths (STH) are the worms which transmitted through the soil. The pe... more ABSTRACTSoil-transmitted helminths (STH) are the worms which transmitted through the soil. The people of Dusun Semen, Grogol, Kediri have a habit of defecating in the river. Farmers who work in direct contact with water and soil is possible to be infected with STH. The purpose of this study was to determine STH species in water, soils, and farmer's fecal samples. Water samples were collected from three spots of river A and B, soil samples from 43 rice fields, and fecal samples from 50 farmers. Water samples were examined by sedimentation, soil samples by floatation, and fecal samples by Kato-Katz method. The data were then analyzed by Chi square test. Out of 18 water samples, 12 samples (66.7%) were positive, consisted of 4 samples (22.2%) contained of Ascaris lumbricoides and 8 samples (44.4%) contained Trichuris trichiura. Total soil samples were 129, where 8 samples (6.2%) were positive for A. lumbricoides, and 10 samples (7.7%) for T. trichiura. Out of 50 villagers, only 39 ...

Research paper thumbnail of Description of Faecal Culture Results in Diarrhea Patient Due to Antibiotic Use

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

DESCRIPTION OF FAECAL CULTURE RESULTS IN DIARRHEA PATIENT DUE TO ANTIBIOTIC USE 1Suci Tresna, IG... more DESCRIPTION OF FAECAL CULTURE RESULTS IN DIARRHEA PATIENT DUE TO ANTIBIOTIC USE 1Suci Tresna, IGAA Putri Sri Rejeki1 Puspa Wardhani1 1Clinical Pathology Spesialization Programme, Department of Clinical Pathology Faculty of Medicine Airlangga University-DR Soetomo Hospital Surabaya 2Department of Clinical Pathology, Faculty of Medicine Airlangga UniversityDr. Soetomo Hospital, Surabaya ABSTRACT Background : Diarrhea infection is common in developing countries and causes death of around 3 million people every year. Diarrhea is also the second leading cause of death in infants. Riskesdas in 2013 showed 30,775 cases of diarrhea. Causes such as bacterial infections Salmonella, shigella, Vibrio, Entamoeba and Yersinia. Other influences can occur due to viral and fungal infections. Diarrhea is a nosocomial infection that is common in hospitalized patients due to long-term use of antibiotics caused by Clostridium difficile. This study was a follow-up study of diarrhea patients who rec...

Research paper thumbnail of Detection of Hearing Impairment Associated with Positive Rubella Antibody Test in Neonatal Intensive Care Unit Dr. Soetomo Hopsital

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Hearing loss in newborns or congenital deafness can be caused by the development of parts of the ... more Hearing loss in newborns or congenital deafness can be caused by the development of parts of the auditory system. Congenital deafness is often associated with infections such as toxoplasmosis, rubella, cytomegalovirus (CMV), and herpes (TORCH). Deafness is very difficult to find early. Examination of inspection methods that are easy and fast. Efforts to conduct early detection are determined through the Newborn Hearing Screening (NHS) program. Otoacoustic Emission (OAE) and Automated Auditory Brainstem Response (AABR) checks are raw materials for early detection. Congenital deafness often occurs with pregnancy infections with viruses such as rubella. Rubella infection during pregnancy, especially during the first trimester often causes Congenital Rubella Syndrome (CRS). Rubella infection is often double with other causes, namely Toxoplasama, CMV and Herpes. Serological examination can be done by examining Rubella IgG and IgM antibodies. Examination of rubella infection with serologi...

Research paper thumbnail of Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia

Journal of Tropical Biodiversity and Biotechnology

An urban park is potentially a source of vector-borne disease transmission due to it being a natu... more An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F15...

Research paper thumbnail of Profil Virus Dengue DI Surabaya Tahun 2008–2009

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Four serotypes of dengue viruses (DENV) 1–4 are mosquito-borne human pathogens that cause widespr... more Four serotypes of dengue viruses (DENV) 1–4 are mosquito-borne human pathogens that cause widespread epidemics withconsiderable morbidity and mortality. The aim of this study was to evaluate the dengue serotypes profile, which were circulating inSurabaya. This research has been carried out consisting of 360 samples from patients with dengue virus infections, according the WorldHealth Organization (WHO) criteria. These sera were collected from patients Dr. Soetomo Hospital and private laboratory in Surabayafrom 2008–2009. From 360 samples, 68 samples (18.9%) were undifferentiated fever, 53 samples (14.7%) were dengue fever, 239samples (66.4%) were dengue hemorrhagic fever. From 58 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) samples, 25samples (43%) were positive, consisting of 52% DEN-2, 20% DEN-1, 16% DEN-3 and 12% DEN-4. These results showed that fourserotypes are circulating in Indonesia, dominated by DEN-2, followed by DEN-1, DEN-3 and DEN-4.

Research paper thumbnail of Inhibitory Activity of Cobalt(II)–Morin Complex Against the Replication of Dengue Virus Type 2

Indonesian Journal of Tropical and Infectious Disease

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease... more Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. Antidengue treatments are urgently required to control the emergence of dengue. DENV is a mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in the tropical and sub-tropical areas and transmitted to humans by Aedes agypty and Aedes albopictus. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is an important alternative for the characterization and development of drugs. Metal–organic compounds were reported to exhibit fungicidal, bactericidal, and antiviral activities its inhibitory activity was not significant, at high concentration it was more toxic to replicating cells than to stationary cell monolayers of Vero cells. The aim of this study is to investigate the antiviral effects of Cobalt(II)–Morin com...

Research paper thumbnail of Suppressive effect of goat bile in Plasmodium berghei ANKA infection in mice

Veterinary World

Background and Aim: Some individuals in Indonesia consume intact goat gallbladder to prevent and ... more Background and Aim: Some individuals in Indonesia consume intact goat gallbladder to prevent and treat malaria. The acute and subacute toxicity tests of goat bile (GB) have shown mild diarrhea in mice. Therefore, this study aimed to evaluate the suppressive effect of GB on parasitemia, splenomegaly, hepatomegaly, and blood biochemistry to assess liver and kidney function in BALB/c mice infected with Plasmodium berghei ANKA. Materials and Methods: Fifty healthy mice were infected with P. berghei ANKA and divided into five groups. Mice in three groups were administered 0.5 mL of 25%, 50%, or 100% of GB by gavage. Animals in Group 4 were administered 187.2 mg/kg BW of dihydroartemisinin-piperaquine phosphate as a positive control (POS Group). Mice in fifth group were administered sterile water as negative (NEG) controls. Further, 30 uninfected mice were divided into groups 6-8 and administered GB as were mice in the first three groups. Group 9 included 10 uninfected and untreated anima...

Research paper thumbnail of The Association of Blood Sugar Nuchter and Total Cholesterol Serum Levels with Platelet Hyperaggregation in Patients with Peripheral Diabetic Neuropathy

Research paper thumbnail of Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF)

Majalah Obstetri & Ginekologi

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer... more Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia.

Research paper thumbnail of Immature granulocyte and mean platelet volume as a predictor of 30-day postoperative mortality in patients with sepsis caused by peritonitis

Bali Journal of Anesthesiology

Research paper thumbnail of Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

Indonesian Journal of Tropical and Infectious Disease

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease... more Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many efforts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitor...

Research paper thumbnail of Correlation of Neutrophils/Lymphoctes Ratio and C-Reactive Protein in Sepsis Patients

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Sepsis merupakan penyebab umum dari kesakitan dan kematian di seluruh dunia. Diagnosis yang cepat... more Sepsis merupakan penyebab umum dari kesakitan dan kematian di seluruh dunia. Diagnosis yang cepat dan tepat sangatdiperlukan. Salah satu respons fisiologis pada sistem imunitas terhadap inflamasi sistemik adalah peningkatan jumlah neutrofil danpenurunan jumlah limfosit. Rasio neutrofil/limfosit telah diketahui sebagai petanda inflamasi yang cepat, sederhana dan murah. Tujuanpenelitian ini adalah membuktikan adanya kenasaban antara rasio neutrofil/limfosit dan c-reactive protein di pasien sepsis. Penelitianbersifat ptong lintang observasional, dilakukan masa waktu Januari hingga Juni 2015. Sampel penelitian terdiri dari 42 pasien sepsisyang dirawat di Instalasi Rawat Darurat RSUD Dr. Soetomo Surabaya. Semua sampel dilakukan pemeriksaan jumlah limfosit, neutrofil,CRP. Hasil dianalisis menggunakan uji kenasaban Spearman’s. Rasio neutrofil/limfosit berkisar antara 3,42–57,47 (21,74±14,1). KadarCRP antara 1,22 mg/L–361,86 mg/L (158 mg/L±97,4 mg/L). Hasil penelitian tidak terdapat kenasab...

Research paper thumbnail of Comparison Results of Analytical Profile Index and Disc Diffusion Antimicrobial Susceptibility Test to Technical Dedicated Reasonable 300B Method

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Angka kematian infeksi aliran darah cukup tinggi, berkisar 20–50%. Patogen penyebab dapat dibukti... more Angka kematian infeksi aliran darah cukup tinggi, berkisar 20–50%. Patogen penyebab dapat dibuktikan dengan pemeriksaan kulturdarah yang dilanjutkan dengan uji kepekaan antibiotika. Metode pemeriksaan dapat dilakukan secara manual atau automatis baiksemiautomatis ataupun automatis penuh. Metode manual relatif tidak memerlukan biaya yang besar dibandingkan metode automatisasi.Penelitian ini merupakan analisis observasional dengan desain potong lintang. Metode identifikasi manual memakai metode API danuji kepekaan antibiotika metode difusi cakram antibiotika Kirby Bauer. Kedua metode ini dibandingkan dengan metode semiautomatisTDR-300B. Metode automatis penuh VITEK 2 digunakan sebagai metode rujukan untuk menilai kinerja metode konvensional dansemiautomatis. Bakteri penyebab infeksi aliran darah didominasi Gram negatif kebanyakan Eschericia coli dan Klebsiella pneumonia.Ketepatan metode identifikasi API terhadap VITEK 2 sebesar 87,87%, ketepatan identifikasi metode TDR-300B terhadap m...

Research paper thumbnail of Kemampuan Uji Tabung Widal Menggunakan Antigen Import Dan Antigen Lokal

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Despite of its higher specificity than Widal-slide test, Widal-tube test is not widely used by me... more Despite of its higher specificity than Widal-slide test, Widal-tube test is not widely used by medical laboratories because it is not practical and each laboratory has to produce their own antigens. The Laboratory must have sufficient microbiology equipments and reagents to produce antigens. REMEL® provides ‘ready for use’ antigens to perform Widal-tube test. To Compare and correlate Widal-tube test using REMEL® imported antigens and local antigens (produced by lab. Clinical pathology Dr. Soetomo hospital). The samples were tested by Widal-tube test, using REMEL® and local antigens, comparing Salmonella typhi (ST) O antigen, ST H antigen, S. paratyphi (SP) A H antigen, and SP B H antigen for each method, with twofold dilution from 1/40 until 1/1280. The number of samples was 55. The results are defined as pathologic (above cut-off value) and non pathologic (below cut-off value). REMEL® ST O antigen had a significant correlation to local antigens (r = 0.665, p < 0.01). REMEL® ST H...

Research paper thumbnail of AST, ALT and Albumin Level in Chronic Hepatitis B Patients with and without Complications of Cirrhosis and Hepatocellular Carcinoma

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carci... more Complications of Chronic Hepatitis B (CHB) infection are liver cirrhosis and Hepatocellular Carcinoma (HCC). Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and albumin may be used as indicators of hepatocyte damage. This study aimed to determine differences between AST, ALT, and albumin in CHB patients without complications with CHB patients with cirrhosis and HCC complications. An analytical cross-sectional study was conducted in March-May 2019 on 62 CHB patients with or without cirrhosis or HCC complications using the total sampling method. AST and ALT were calculated using the Siemens Dimension device and IFCC method. The data were analyzed using independent samples T-test Albumin in CHB patients without complications was higher than CHB patients who had complications of liver cirrhosis (p=0.002). The AST and ALT were not significantly different. Aspartate aminotransferase in CHB with cirrhosis complications differed from CHB patients who had HCC complications ...

Research paper thumbnail of Dengue Virus Serotype 4 Is Responsible for the Outbreak of Dengue in East Java City of Jember, Indonesia

Viruses

Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2;... more Outbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was the least-reported serotype in Indonesia during the last five decades. We recently conducted a molecular epidemiology study of dengue in the Jember regency, East Java province, Indonesia. Dengue is endemic in the region and outbreaks occur annually. We investigated the clinical characteristics and etiology of dengue-like febrile illness in this regency to understand the disease dynamics. A total of 191 patients with clinical symptoms similar to dengue were recruited during an 11-month study in 2019–2020. Children accounted for the majority of cases and dengue burden was estimated in 41.4% of the cases based on NS1 antigen, viral RNA, and IgG/IgM antibody detection with the majority (73.4%) being primary infections. Secondary infection was significantly associated with a higher risk of severe dengue manifestation. All four DENV serotypes were detected in Jember. Str...

Research paper thumbnail of Pengaruh Rendaman Air Terhadap Kualitas DNA pada Sperma dengan STR-CODIS D13S317 dan D21S1

Jurnal Biosains Pascasarjana

AbstrakKriminalitas yang terjadi akibat kejahatan seksual banyak terjadi di mana-mana. Kejahatan ... more AbstrakKriminalitas yang terjadi akibat kejahatan seksual banyak terjadi di mana-mana. Kejahatan ini bisa menimpa beberapa orang dan dari berbagai umur. Dalam kasus ini pasti akan di temukan barang bukti di tempat kejadian perkara. Salah satu bukti akibat kejahatanseksual adalah bercak sperma. Bercak sperma dapat di temukan seperti pada pakaian yang digunakan korban atau pelaku. Dengan keterbatasan waktu yang dimiliki oleh polisi tentunyaperlu cara untuk pengidentifikasi siapa pelaku dari kejahatan tersebut. Sampel bercak sperma akan di isolasi DNA. Isolasi DNA adalah memisahkan DNA yang ada pada sel sperma dengan komponen yang lainnya. Setelah DNA di dapatkan akan di ketahui kadar dankemurniannya. Hasil tersebut bisa di pengaruhi oleh media bercak sperma berada. Faktor- faktor tersebut salah satunya media air dan kain katun. Sifat kain katun adalah mempunyaikemampuan menyerap yang tinggi walaupun dalam keadaan basah sekalipun. Setelah isolasi DNA, tahapan selanjutnya ...

Research paper thumbnail of The incidence of Clostridium Difficile infection in diarrhea patients after receiving antibiotics at Dr. Soetomo Hospital Surabaya

Research paper thumbnail of The Correlation between leucocyte CD64, Immature Granulocyte and Presepsin with Procalcitonin in Bacterial Sepsis Patient

Research paper thumbnail of Acute and subacute toxicity tests of goat bile in BALB/c mice

Veterinary World

Aim: The aim of this study was to investigate the toxicity of goat bile in BALB/c mice since some... more Aim: The aim of this study was to investigate the toxicity of goat bile in BALB/c mice since some Indonesian people consume raw goat gallbladder to treat malaria and increase stamina. Materials and Methods: Acute toxicity test was done in six groups of BALB/c mice using 100%, 50%, 25%, 12.5%, and 6.75% of goat bile and negative control. The death of mice was observed within 14 days. In the subacute toxicity test, the body weight and hematology parameters on day 0 and day 4 post-treatment were evaluated. The mice were closely observed for 28 days before plasma collection for the blood biochemistry evaluation. Results: Mild diarrhea was observed in acute and subacute toxicity tests. No death of mice was observed in acute test. Goat bile did not inhibit the increase of the body weight of mice. A slight reduction in hemoglobin and hematocrit levels in mice treated with 25% and 50% goat bile, however, remained normal in mice treated with 100% goat bile. The red and white blood cell count...

Research paper thumbnail of Contamination of water and soil of rice fields with soil transmitted helminths as source of transmission to farmers in Grogol sub-district, Kediri district

Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya

ABSTRACTSoil-transmitted helminths (STH) are the worms which transmitted through the soil. The pe... more ABSTRACTSoil-transmitted helminths (STH) are the worms which transmitted through the soil. The people of Dusun Semen, Grogol, Kediri have a habit of defecating in the river. Farmers who work in direct contact with water and soil is possible to be infected with STH. The purpose of this study was to determine STH species in water, soils, and farmer's fecal samples. Water samples were collected from three spots of river A and B, soil samples from 43 rice fields, and fecal samples from 50 farmers. Water samples were examined by sedimentation, soil samples by floatation, and fecal samples by Kato-Katz method. The data were then analyzed by Chi square test. Out of 18 water samples, 12 samples (66.7%) were positive, consisted of 4 samples (22.2%) contained of Ascaris lumbricoides and 8 samples (44.4%) contained Trichuris trichiura. Total soil samples were 129, where 8 samples (6.2%) were positive for A. lumbricoides, and 10 samples (7.7%) for T. trichiura. Out of 50 villagers, only 39 ...

Research paper thumbnail of Description of Faecal Culture Results in Diarrhea Patient Due to Antibiotic Use

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

DESCRIPTION OF FAECAL CULTURE RESULTS IN DIARRHEA PATIENT DUE TO ANTIBIOTIC USE 1Suci Tresna, IG... more DESCRIPTION OF FAECAL CULTURE RESULTS IN DIARRHEA PATIENT DUE TO ANTIBIOTIC USE 1Suci Tresna, IGAA Putri Sri Rejeki1 Puspa Wardhani1 1Clinical Pathology Spesialization Programme, Department of Clinical Pathology Faculty of Medicine Airlangga University-DR Soetomo Hospital Surabaya 2Department of Clinical Pathology, Faculty of Medicine Airlangga UniversityDr. Soetomo Hospital, Surabaya ABSTRACT Background : Diarrhea infection is common in developing countries and causes death of around 3 million people every year. Diarrhea is also the second leading cause of death in infants. Riskesdas in 2013 showed 30,775 cases of diarrhea. Causes such as bacterial infections Salmonella, shigella, Vibrio, Entamoeba and Yersinia. Other influences can occur due to viral and fungal infections. Diarrhea is a nosocomial infection that is common in hospitalized patients due to long-term use of antibiotics caused by Clostridium difficile. This study was a follow-up study of diarrhea patients who rec...

Research paper thumbnail of Detection of Hearing Impairment Associated with Positive Rubella Antibody Test in Neonatal Intensive Care Unit Dr. Soetomo Hopsital

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Hearing loss in newborns or congenital deafness can be caused by the development of parts of the ... more Hearing loss in newborns or congenital deafness can be caused by the development of parts of the auditory system. Congenital deafness is often associated with infections such as toxoplasmosis, rubella, cytomegalovirus (CMV), and herpes (TORCH). Deafness is very difficult to find early. Examination of inspection methods that are easy and fast. Efforts to conduct early detection are determined through the Newborn Hearing Screening (NHS) program. Otoacoustic Emission (OAE) and Automated Auditory Brainstem Response (AABR) checks are raw materials for early detection. Congenital deafness often occurs with pregnancy infections with viruses such as rubella. Rubella infection during pregnancy, especially during the first trimester often causes Congenital Rubella Syndrome (CRS). Rubella infection is often double with other causes, namely Toxoplasama, CMV and Herpes. Serological examination can be done by examining Rubella IgG and IgM antibodies. Examination of rubella infection with serologi...