Alejandra Roque | Universidad Nacional Autónoma de México (original) (raw)

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Papers by Alejandra Roque

International Journal of Systematic and Evolutionary Microbiology, 2004

Three Gram-negative, small, motile, rod-shaped bacteria were isolated from Artemia sp. and sea wa... more Three Gram-negative, small, motile, rod-shaped bacteria were isolated from Artemia sp. and sea water in Barcelona, Spain, during 1990 and. They were fermentative, oxidase-positive, sensitive to vibriostatic agent O/129, arginine dihydrolase-positive, lysine and ornithine decarboxylase-negative and grew in the absence of NaCl. They differed from phenotypically related species by their ability to grow at 4 6C and utilize L-rhamnose. Cloning of the 16S rRNA gene of the type strain produced two different 16S rRNA gene sequences, which differed by 15 bases (0?99 %); comparison of these sequences with those deposited in GenBank showed close relationships with Vibrio proteolyticus (97?6 % similarity), Vibrio diazotrophicus (97?9 %), Vibrio campbellii (96?8 %) and Vibrio alginolyticus (96?8 %), among others. DNA-DNA hybridization levels with the closest phylogenetically related Vibrio species were <26?4 %. Sufficient evidence is provided to support the identity of the three strains analysed as members of a novel species of the genus Vibrio, for which the name Vibrio hispanicus sp. nov. is proposed, with the type strain LMG 13240 T (=CAIM 525 T =VIB 213 T ).

International Journal of Systematic and Evolutionary Microbiology, 2010

Six Gram-negative coccobacilli were isolated from Pacific oysters (Crassostrea gigas) from Mexico... more Six Gram-negative coccobacilli were isolated from Pacific oysters (Crassostrea gigas) from Mexico and haemolymph of spider crabs (Maja brachydactyla) from Spain. All of the isolates grew as small green colonies on thiosulphate-citrate-bile salts-sucrose (TCBS) agar and were facultatively anaerobic, oxidase-positive and sensitive to the vibriostatic agent O/129. Repetitive palindromic PCR analysis revealed a high degree of genomic homogeneity among the isolates. Several phenotypic traits differentiated the isolates from the type strains of species of the genus Photobacterium. DNA-DNA relatedness between two representative isolates and their closest phylogenetic neighbours by 16S rRNA gene sequence similarity, Photobacterium aplysiae CAIM 14 T and Photobacterium frigidiphilum CAIM 20 T , was 44.01-53.85 %. We propose a novel species of the genus Photobacterium to accommodate the six isolates, with the name Photobacterium swingsii sp. nov. The type strain is CAIM 1393 T (5CECT 7576 T ).

incluyendo la exploración geotécnica, el diseño por capacidad de carga, así como el cálculo de as... more incluyendo la exploración geotécnica, el diseño por capacidad de carga, así como el cálculo de asentamientos. Se incluye el diseño y ejecución de una prueba de carga de compresión y una de tensión, en una pila construida para este propósito, así como los resultados obtenidos, comparándolos con los del diseño original.

International Journal of Systematic and Evolutionary Microbiology, 2004

Three Gram-negative, small, motile, rod-shaped bacteria were isolated from Artemia sp. and sea wa... more Three Gram-negative, small, motile, rod-shaped bacteria were isolated from Artemia sp. and sea water in Barcelona, Spain, during 1990 and. They were fermentative, oxidase-positive, sensitive to vibriostatic agent O/129, arginine dihydrolase-positive, lysine and ornithine decarboxylase-negative and grew in the absence of NaCl. They differed from phenotypically related species by their ability to grow at 4 6C and utilize L-rhamnose. Cloning of the 16S rRNA gene of the type strain produced two different 16S rRNA gene sequences, which differed by 15 bases (0?99 %); comparison of these sequences with those deposited in GenBank showed close relationships with Vibrio proteolyticus (97?6 % similarity), Vibrio diazotrophicus (97?9 %), Vibrio campbellii (96?8 %) and Vibrio alginolyticus (96?8 %), among others. DNA-DNA hybridization levels with the closest phylogenetically related Vibrio species were <26?4 %. Sufficient evidence is provided to support the identity of the three strains analysed as members of a novel species of the genus Vibrio, for which the name Vibrio hispanicus sp. nov. is proposed, with the type strain LMG 13240 T (=CAIM 525 T =VIB 213 T ).

International Journal of Systematic and Evolutionary Microbiology, 2010

Six Gram-negative coccobacilli were isolated from Pacific oysters (Crassostrea gigas) from Mexico... more Six Gram-negative coccobacilli were isolated from Pacific oysters (Crassostrea gigas) from Mexico and haemolymph of spider crabs (Maja brachydactyla) from Spain. All of the isolates grew as small green colonies on thiosulphate-citrate-bile salts-sucrose (TCBS) agar and were facultatively anaerobic, oxidase-positive and sensitive to the vibriostatic agent O/129. Repetitive palindromic PCR analysis revealed a high degree of genomic homogeneity among the isolates. Several phenotypic traits differentiated the isolates from the type strains of species of the genus Photobacterium. DNA-DNA relatedness between two representative isolates and their closest phylogenetic neighbours by 16S rRNA gene sequence similarity, Photobacterium aplysiae CAIM 14 T and Photobacterium frigidiphilum CAIM 20 T , was 44.01-53.85 %. We propose a novel species of the genus Photobacterium to accommodate the six isolates, with the name Photobacterium swingsii sp. nov. The type strain is CAIM 1393 T (5CECT 7576 T ).

incluyendo la exploración geotécnica, el diseño por capacidad de carga, así como el cálculo de as... more incluyendo la exploración geotécnica, el diseño por capacidad de carga, así como el cálculo de asentamientos. Se incluye el diseño y ejecución de una prueba de carga de compresión y una de tensión, en una pila construida para este propósito, así como los resultados obtenidos, comparándolos con los del diseño original.