LUZ ALCANTARA | UNAM Universidad Nacional Autónoma de México (original) (raw)
Papers by LUZ ALCANTARA
Journal of Visualized Experiments, 2015
Medwave, 2011
TNF-α e IL-1β ¿son los causantes del daño de la cicatriz patológica posquemadura al año de evoluc... more TNF-α e IL-1β ¿son los causantes del daño de la cicatriz patológica posquemadura al año de evolución?
Journal of Nutrition & Food Sciences, 2015
Biochemical and Biophysical Research Communications, 2013
Nukbone showed to be a good scaffold for adhesion, proliferation and differentiation of stem cells.
Oxidative stress has been implicated in various skin diseases through the generation of reacti... more Oxidative stress has been implicated in various skin diseases through the generation of reactive oxygen species and the depletion of endogenous antioxidant systems. Evidence exists indicating that oxidative stress injury of the skin caused by UVB irradiation is mediated predominantly by reactive oxygen species (ROS) immediately after irradiation and by reactive nitrogen species (RNS) at later points. We investigated the protective effect of peptides (albumin and hydrolysates) isolated from Amaranthus (pA) against UVB irradiation, lipid peroxidation (malondialdehyde, MDA) and endogenous antioxidant defense enzymes such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT). Supernatant levels of pro-inflammatory and anti-inflammatory cytokines were also investigated. We used human skin organ culture as an in vitro model via 24, 36 and 48 hrs of culture. The pA has a significant protective effect via the inhibition of damaged caused by UVB irradiation. The treatment of skin with pA inhibited the UVB-induced lipid peroxidation. Additionally, this pA-inhibited UVB-induced depletion of antioxidant defense components, such as, CAT, SOD, and GPX, and the final antioxidant treatment also reduced levels of pro-inflammatory cytokines: IL-1beta, IL-12, TNF and INF-gamma. We conclude that pA could be useful in attenuating UVB-induced oxidative stress and mitigating skin diseases in human skin due to antioxidant properties.
![Research paper thumbnail of [Stem cells biobanks for cell therapy. A reality in Mexico.]](https://attachments.academia-assets.com/41528350/thumbnails/1.jpg)
](Revista medica del Instituto Mexicano del Seguro Social
R ecientemente ha crecido el interés en el estudio de las células troncales como parte de la medi... more R ecientemente ha crecido el interés en el estudio de las células troncales como parte de la medicina basada en evidencias. Este campo es dinámico y se mueve rápidamente de la ciencia básica a la aplicación clínica, por lo que la obtención y el almacenamiento de muestras de tejido humano (práctica presente en la medicina desde hace siglos) constituyen una actividad del biobanco.
Cell and Tissue Research, 2006
The gonadal development of chicken embryo is regulated by hormones and growth factors. Transformi... more The gonadal development of chicken embryo is regulated by hormones and growth factors. Transforming growth factor beta (TGF-beta) isoforms may play a critical role in the regulation of growth in chicken gonads. We have investigated the effect of the TGF-beta isoforms on the number of germ and somatic cells in the ovary of the chicken embryo. Ovaries were obtained from chicken embryos at 9 days of incubation. They were organ-cultured for 72 h in groups treated with TGF-beta1, TGF-beta2, soluble betaglycan, TGF-beta1 plus soluble betaglycan, or TGF-beta2 plus soluble betaglycan, and untreated (control). TGF-beta1 and TGF-beta2 diminished the somatic cell number in the ovary of the chicken embryo at this age by inhibiting the proliferation of the somatic cells without increasing apoptosis. On the other hand, TGF-beta1 and TGF-beta2 did not affect the number of germ cells in the cultured ovary. The capacity of TGF-beta1 and TGF-beta2 to diminish the number of somatic cells in the ovary was blocked with soluble betaglycan, a natural TGF-beta antagonist. However, changes in the location of germ cells within the ovary suggested that TGF-beta promoted the migration of the germ cells from the ovarian cortex to the medulla. Thus, TGF-beta affects germ and somatic cells in the ovary of the 9-day-old chicken embryo and inhibits the proliferation of somatic cells.
Burns, 2012
Post-burn hypertrophic scars are characterized by increased collagen synthesis and hyperplasia, a... more Post-burn hypertrophic scars are characterized by increased collagen synthesis and hyperplasia, and may be associated with erythema, pain, dysesthesia, pruritus, and skin border elevation. Although the etiopathogenesis of hypertrophic scarring remains unclear, proinflammatory and profibrogenic cytokines are known to play an important role in general skin dysfunction. This study assessed mRNA expression, proteins, and type I receptors of tumor necrosis factor-alpha (TNF-α) and interleukin 1-beta (IL-1β) in normal skin, normotrophic and post-burn hypertrophic scars. Skin biopsies were obtained from 10 hypertrophic and 9 normotrophic scars, and 4 normal skin sites. Only post-burn scars covering more than 10% of the body were included. Ex vivo histopathological analysis evaluated scar maturity, in situ hybridization assessed mRNA expression, and cytokine protein and cytokine/cell colocalization were performed using single- and double-label immunohistochemistry, respectively. IL-1β is overexpressed in hypertrophic scars at the post-transcriptional level, associated primarily with keratinocytes and CD1a(+) cells. Type I receptors for TNF-α are overexpressed in blood vessels of hypertrophic scars. The coordinated overexpression of IL-1β and TNF-α type I receptor may maintain the fibrogenic phenotypes of hypertrophic scars, even those in "remission".
Biochemical and Biophysical Research Communications, 2013
Nukbone showed to be a good scaffold for adhesion, proliferation and differentiation of stem cells.
Journal of Visualized Experiments, 2015
Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when ... more Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs bovine bone matrix Nukbone (NKB) as a scaffold. Thus, the application of MSCs in repair and tissue regeneration processes depends principally on the efficient implementation of the techniques for placing these cells in a host tissue. For this reason, the design of biomaterials and cellular scaffolds has gained importance in recent years because the topographical characteristics of the selected scaffold must ensure adhesion, proliferation and differentiation into the desired cell lineage in the microenvironment of the injured tissue. This option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) employs bovine bone matrix as a cellular scaffold and is an efficient culture technique because the cells respond to the topographic characteristics of the bovine bone matrix Nukbone (NKB), i.e., spreading on the surface, macroporous covering and colonizing the depth of the biomaterial, after the cell isolation process. We present the procedure for isolating and culturing MSCs on a bovine matrix.
Journal of Visualized Experiments, 2015
Medwave, 2011
TNF-α e IL-1β ¿son los causantes del daño de la cicatriz patológica posquemadura al año de evoluc... more TNF-α e IL-1β ¿son los causantes del daño de la cicatriz patológica posquemadura al año de evolución?
Journal of Nutrition & Food Sciences, 2015
Biochemical and Biophysical Research Communications, 2013
Nukbone showed to be a good scaffold for adhesion, proliferation and differentiation of stem cells.
Oxidative stress has been implicated in various skin diseases through the generation of reacti... more Oxidative stress has been implicated in various skin diseases through the generation of reactive oxygen species and the depletion of endogenous antioxidant systems. Evidence exists indicating that oxidative stress injury of the skin caused by UVB irradiation is mediated predominantly by reactive oxygen species (ROS) immediately after irradiation and by reactive nitrogen species (RNS) at later points. We investigated the protective effect of peptides (albumin and hydrolysates) isolated from Amaranthus (pA) against UVB irradiation, lipid peroxidation (malondialdehyde, MDA) and endogenous antioxidant defense enzymes such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT). Supernatant levels of pro-inflammatory and anti-inflammatory cytokines were also investigated. We used human skin organ culture as an in vitro model via 24, 36 and 48 hrs of culture. The pA has a significant protective effect via the inhibition of damaged caused by UVB irradiation. The treatment of skin with pA inhibited the UVB-induced lipid peroxidation. Additionally, this pA-inhibited UVB-induced depletion of antioxidant defense components, such as, CAT, SOD, and GPX, and the final antioxidant treatment also reduced levels of pro-inflammatory cytokines: IL-1beta, IL-12, TNF and INF-gamma. We conclude that pA could be useful in attenuating UVB-induced oxidative stress and mitigating skin diseases in human skin due to antioxidant properties.
Revista medica del Instituto Mexicano del Seguro Social
R ecientemente ha crecido el interés en el estudio de las células troncales como parte de la medi... more R ecientemente ha crecido el interés en el estudio de las células troncales como parte de la medicina basada en evidencias. Este campo es dinámico y se mueve rápidamente de la ciencia básica a la aplicación clínica, por lo que la obtención y el almacenamiento de muestras de tejido humano (práctica presente en la medicina desde hace siglos) constituyen una actividad del biobanco.
Cell and Tissue Research, 2006
The gonadal development of chicken embryo is regulated by hormones and growth factors. Transformi... more The gonadal development of chicken embryo is regulated by hormones and growth factors. Transforming growth factor beta (TGF-beta) isoforms may play a critical role in the regulation of growth in chicken gonads. We have investigated the effect of the TGF-beta isoforms on the number of germ and somatic cells in the ovary of the chicken embryo. Ovaries were obtained from chicken embryos at 9 days of incubation. They were organ-cultured for 72 h in groups treated with TGF-beta1, TGF-beta2, soluble betaglycan, TGF-beta1 plus soluble betaglycan, or TGF-beta2 plus soluble betaglycan, and untreated (control). TGF-beta1 and TGF-beta2 diminished the somatic cell number in the ovary of the chicken embryo at this age by inhibiting the proliferation of the somatic cells without increasing apoptosis. On the other hand, TGF-beta1 and TGF-beta2 did not affect the number of germ cells in the cultured ovary. The capacity of TGF-beta1 and TGF-beta2 to diminish the number of somatic cells in the ovary was blocked with soluble betaglycan, a natural TGF-beta antagonist. However, changes in the location of germ cells within the ovary suggested that TGF-beta promoted the migration of the germ cells from the ovarian cortex to the medulla. Thus, TGF-beta affects germ and somatic cells in the ovary of the 9-day-old chicken embryo and inhibits the proliferation of somatic cells.
Burns, 2012
Post-burn hypertrophic scars are characterized by increased collagen synthesis and hyperplasia, a... more Post-burn hypertrophic scars are characterized by increased collagen synthesis and hyperplasia, and may be associated with erythema, pain, dysesthesia, pruritus, and skin border elevation. Although the etiopathogenesis of hypertrophic scarring remains unclear, proinflammatory and profibrogenic cytokines are known to play an important role in general skin dysfunction. This study assessed mRNA expression, proteins, and type I receptors of tumor necrosis factor-alpha (TNF-α) and interleukin 1-beta (IL-1β) in normal skin, normotrophic and post-burn hypertrophic scars. Skin biopsies were obtained from 10 hypertrophic and 9 normotrophic scars, and 4 normal skin sites. Only post-burn scars covering more than 10% of the body were included. Ex vivo histopathological analysis evaluated scar maturity, in situ hybridization assessed mRNA expression, and cytokine protein and cytokine/cell colocalization were performed using single- and double-label immunohistochemistry, respectively. IL-1β is overexpressed in hypertrophic scars at the post-transcriptional level, associated primarily with keratinocytes and CD1a(+) cells. Type I receptors for TNF-α are overexpressed in blood vessels of hypertrophic scars. The coordinated overexpression of IL-1β and TNF-α type I receptor may maintain the fibrogenic phenotypes of hypertrophic scars, even those in "remission".
Biochemical and Biophysical Research Communications, 2013
Nukbone showed to be a good scaffold for adhesion, proliferation and differentiation of stem cells.
Journal of Visualized Experiments, 2015
Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when ... more Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs bovine bone matrix Nukbone (NKB) as a scaffold. Thus, the application of MSCs in repair and tissue regeneration processes depends principally on the efficient implementation of the techniques for placing these cells in a host tissue. For this reason, the design of biomaterials and cellular scaffolds has gained importance in recent years because the topographical characteristics of the selected scaffold must ensure adhesion, proliferation and differentiation into the desired cell lineage in the microenvironment of the injured tissue. This option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) employs bovine bone matrix as a cellular scaffold and is an efficient culture technique because the cells respond to the topographic characteristics of the bovine bone matrix Nukbone (NKB), i.e., spreading on the surface, macroporous covering and colonizing the depth of the biomaterial, after the cell isolation process. We present the procedure for isolating and culturing MSCs on a bovine matrix.