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Papers by Oselys Rodriguez Justo

The Journal of pharmacy and pharmacology, 2005

The primary goal of this study was the production of liposomes encapsulating kanamycin for drug a... more The primary goal of this study was the production of liposomes encapsulating kanamycin for drug administration by inhalation. The selected drug is indicated for multiresistant tuberculosis, and administration through inhalation allows both local delivery of the drug to the lungs and systemic therapy. The ethanol injection method used for the liposome production is easily scaled up and is characterized by simplicity and low cost. Vesicles were prepared using different lipid compositions, including hydrogenated soybean phosphatidylcholine and cholesterol (SPC/Chol), egg phosphatidylcholine and cholesterol (EPC/Chol), distearoyl phosphatidylcholine and cholesterol (DSPC/Chol), distearoyl phosphatidylcholine, dimyristoyl phosphatidylethanolamine and cholesterol (DSPC/DMPE/Chol), dipalmitoyl phosphatidylcholine and cholesterol (DPPC/Chol) and dipalmitoyl phosphatidylcholine, dipalmitoyl phosphatidylglycerol and cholesterol (DPPC/DPPG/Chol). The effects of different operational conditions for vesicle production and drug encapsulation were evaluated, aiming at a compromise between final process cost and suitable vesicle characteristics. The best performance concerning drug incorporation was achieved with the DSPC/Chol system, although its production cost was considerably larger than that of the natural lipids formulations. Encapsulation efficiencies up to 63% and final drug to lipid molar ratios up to 0.1 were obtained for SPC/Chol vesicles presenting mean diameters of 132 nm incubated at 60 degrees C with the drug for 60 min at an initial drug-to-lipid molar ratio of 0.16.

Química Nova, 2008

Recebido em 23/10/07; aceito em 30/4/08; publicado na web em 19/9/08 EVALUATION OF THE ANTIOXIDAN... more Recebido em 23/10/07; aceito em 30/4/08; publicado na web em 19/9/08 EVALUATION OF THE ANTIOXIDANT POTENTIAL OF PLANT EXTRACTS OBTAINED BY SUPERCRITICAL FLUID EXTRACTION. The aim of this work was to evaluate the antioxidant properties of ginger and rosemary extracts, obtained by supercritical extraction. The extracts were characterized by HPLC, GC-MS, phenolic compounds content and antioxidant activity. The main active compounds were identified and high content of phenolic compounds was observed. The extracts presented high antioxidant activity against the free radicals ABTS •+ (350 and 200 mM Trolox/g, for ginger and rosemary, respectively) and DPPH • (145 and 80 mM Trolox/g, for ginger and rosemary, respectively). These results suggested that the attained extracts are potential substitutes of synthetic antioxidants used in chemical, food and pharmaceutical industries.

Liposomal encapsulation of tuberculostatic drugs can potentially increase their therapeutic index... more Liposomal encapsulation of tuberculostatic drugs can potentially increase their therapeutic index. The incorporation of isoniazid, pyrazinamide, rifampicin, ethionamide, and streptomycin in extruded distearoylphosphatidylcholine/cholesterol liposomes designed for administration through inhalation was evaluated. Ethionamide and rifampicin were incorporated during lipid film formation, whereas solutions of the remaining drugs were used to hydrate preformed lipid bilayers. Final drug to lipid ratios around 0.3 were achieved for isoniazid and pyrazinamide, and mean vesicle diameters varied from 286 to 329 nm. No expressive drug leakage or mean vesicle diameter changes occurred during 3 weeks. No significant incorporation was achieved for streptomycin, ethionamide, and rifampicin.

Six fungi strains (M. anisopliae 3935, 4516, 4819, PL57, PL43 and M. flavoviride CG291) were stud... more Six fungi strains (M. anisopliae 3935, 4516, 4819, PL57, PL43 and M. flavoviride CG291) were studied regarding their ability to produce an anticancer indolizidine alkaloid. The culture process was carried out in Shaken flask at 26ºC and 200 rpm using three different culture medium containing oat meal extract supplemented with glucose and DL-lysine or Czapek culture medium. The mycelial extracts

BACKGROUND: In this work recombinant gp-41 protein production by E. coli in an unconventional bio... more BACKGROUND: In this work recombinant gp-41 protein production by E. coli in an unconventional bioreactor coupled with a magnetic field generator was studied. The submerged fermentation process was carried out by fed-batch operation, recycling the culture medium externally through a stainless steel spiral U-shape tube inserted on the inside of a magnetic field generator. The exposure time and magnetic field induction were varied in a range from 1 to 12 h and 10 to 100 mT, respectively, according to a central composite design with centered face. RESULTS: It was found that the inhibitory or stimulatory effect of magnetic field on cell growth and protein production depended on exposure time and magnetic field strength. The production of recombinant gp41 protein was 20% higher than the control. CONCLUSION: The procedure here presented could be an easy-to-use approach to improve the efficiency of recombinant protein production by E. coli.

Liposomes have been extensively used as carriers of several compounds, due to their structural ve... more Liposomes have been extensively used as carriers of several compounds, due to their structural versatility in terms of size, composition, surface charge, lamellarity, and facility to incorporate hydrophilic and hydrophobic substances.

Biofuels in Brazil, 2014

ABSTRACT The use of renewable fuels, an alternative that reduces the generation of greenhouse gas... more ABSTRACT The use of renewable fuels, an alternative that reduces the generation of greenhouse gases, is one proposal to mitigate the effects that contribute to global warming. Brazil is the fourth largest producer of biodiesel in the world, and growth expectations of the productive capacities have generated huge technological and environmental challenges. In this context, this chapter discusses some of the relevant aspects of biodiesel production in Brazil, including sustainability of raw materials, conventional technology limitations, and further presents technological alternatives as strategies that will guide the future directions which can result in processes with greater environmental and economic returns.

Química Nova, 2009

Recebido em 28/4/08; aceito em 19/1/09; publicado na web em 28/7/09 EFFECT OF THE PRECURSOR ADDIT... more Recebido em 28/4/08; aceito em 19/1/09; publicado na web em 28/7/09 EFFECT OF THE PRECURSOR ADDITION ON THE ANTICANCER ALKALOID PRODUCTION USING EXPERIMENTAL DESIGN METHODOLOGY. The effect of precursors on the anticancer alkaloid production by submerged fermentation using M. anisopliae 3935 was studied, according to complete experimental design 2 2 with three central points. The results showed that lysine was the most important variable, however, when both lysine and glucose were added to the fermentation medium, the alkaloid production reached, approximately, 17 mg L -1 after 120 hours of fermentation. Then, the scale-up of the process was carried out and these results were confirmed. Finally, 35 mg L -1 of alkaloid at 192 h were attained after increment of added aminoacid lysine.

Process Biochemistry, 2006

The present study was designed to investigate the effect of extremely low frequency (ELF) magneti... more The present study was designed to investigate the effect of extremely low frequency (ELF) magnetic field (MF) on spinal cord injury (SCI)-induced osteoporosis in rats. Adult male Wistar rats (n = 24) were equally divided into sham, SCI, and SCI+MF groups. Complete transection of spinal cord (thoracic 11 vertebra) was surgically performed under anesthesia, whereas in the sham group only laminectomy was done. Post-SCI day 1, rats were either exposed (2 h/d × 8 wk) to ELF-MF (17.96 micro-Tesla, 50 Hz; SCI+MF group) or sham exposed (SCI group). Basso, Beattie, and Bresnahan (BBB) score was recorded weekly. All the rats were sacrificed 8 wk post-SCI; tibia and femur bones were isolated for the analysis of bone mineral content (BMC; total calcium [Ca], phosphorus [P], carbon [C]), bone mineral density (BMD), and biochemical status (osteocalcin, collagen I, alkaline phosphatase). The BBB score decreased post-SCI, which partially recovered after ELF-MF. In SCI rats, there was a statistically significant decrease in BMC, Ca, P, C, BMD, and biochemical level in both the bones as compared with the sham group, which was attenuated in SCI+MF rats except the C content. Electron microscopic study revealed the enhancement of microstructural composition and compactness in cortical and trabecular parts of treated bones. The results suggest that the chronic (2 h/d × 8 wk) ELF-MF exposure (17.96 micro-Tesla, 50 Hz) to SCI rats is effective in attenuating SCI-induced osteoporosis.

Applied Biochemistry and Biotechnology, 2006

The influence of extremely low-frequency (ELF) electromagnetic fields on Escherichia coli culture... more The influence of extremely low-frequency (ELF) electromagnetic fields on Escherichia coli cultures in submerse fermentation was studied. The fermentation processes were carried out recycling the culture medium externally through a stainless steel tube inserted in a magnetic field generator (solenoid). The exposure time and electromagnetic induction were varied in a range of 1 to 12 h and 0.010 to 0.10 T, respectively, according to a Box-Wilson Central Composite Designs of face centered with five central points. Growth of E. coli could be altered (stimulated or inhibited) under magnetic fieldinduced effects. E. coli cultures exposed at 0.1 T during 6.5 h exhibited changes in its viability compared to unexposed cells, which was 100 times higher than the control. The magnetic field generator associated with the cellular suspension recycle is a new way of magnetic treatment in fermentation processes and could be appropriate to industrial scale up.

The Journal of pharmacy and pharmacology, 2005

The primary goal of this study was the production of liposomes encapsulating kanamycin for drug a... more The primary goal of this study was the production of liposomes encapsulating kanamycin for drug administration by inhalation. The selected drug is indicated for multiresistant tuberculosis, and administration through inhalation allows both local delivery of the drug to the lungs and systemic therapy. The ethanol injection method used for the liposome production is easily scaled up and is characterized by simplicity and low cost. Vesicles were prepared using different lipid compositions, including hydrogenated soybean phosphatidylcholine and cholesterol (SPC/Chol), egg phosphatidylcholine and cholesterol (EPC/Chol), distearoyl phosphatidylcholine and cholesterol (DSPC/Chol), distearoyl phosphatidylcholine, dimyristoyl phosphatidylethanolamine and cholesterol (DSPC/DMPE/Chol), dipalmitoyl phosphatidylcholine and cholesterol (DPPC/Chol) and dipalmitoyl phosphatidylcholine, dipalmitoyl phosphatidylglycerol and cholesterol (DPPC/DPPG/Chol). The effects of different operational conditions for vesicle production and drug encapsulation were evaluated, aiming at a compromise between final process cost and suitable vesicle characteristics. The best performance concerning drug incorporation was achieved with the DSPC/Chol system, although its production cost was considerably larger than that of the natural lipids formulations. Encapsulation efficiencies up to 63% and final drug to lipid molar ratios up to 0.1 were obtained for SPC/Chol vesicles presenting mean diameters of 132 nm incubated at 60 degrees C with the drug for 60 min at an initial drug-to-lipid molar ratio of 0.16.

Química Nova, 2008

Recebido em 23/10/07; aceito em 30/4/08; publicado na web em 19/9/08 EVALUATION OF THE ANTIOXIDAN... more Recebido em 23/10/07; aceito em 30/4/08; publicado na web em 19/9/08 EVALUATION OF THE ANTIOXIDANT POTENTIAL OF PLANT EXTRACTS OBTAINED BY SUPERCRITICAL FLUID EXTRACTION. The aim of this work was to evaluate the antioxidant properties of ginger and rosemary extracts, obtained by supercritical extraction. The extracts were characterized by HPLC, GC-MS, phenolic compounds content and antioxidant activity. The main active compounds were identified and high content of phenolic compounds was observed. The extracts presented high antioxidant activity against the free radicals ABTS •+ (350 and 200 mM Trolox/g, for ginger and rosemary, respectively) and DPPH • (145 and 80 mM Trolox/g, for ginger and rosemary, respectively). These results suggested that the attained extracts are potential substitutes of synthetic antioxidants used in chemical, food and pharmaceutical industries.

Liposomal encapsulation of tuberculostatic drugs can potentially increase their therapeutic index... more Liposomal encapsulation of tuberculostatic drugs can potentially increase their therapeutic index. The incorporation of isoniazid, pyrazinamide, rifampicin, ethionamide, and streptomycin in extruded distearoylphosphatidylcholine/cholesterol liposomes designed for administration through inhalation was evaluated. Ethionamide and rifampicin were incorporated during lipid film formation, whereas solutions of the remaining drugs were used to hydrate preformed lipid bilayers. Final drug to lipid ratios around 0.3 were achieved for isoniazid and pyrazinamide, and mean vesicle diameters varied from 286 to 329 nm. No expressive drug leakage or mean vesicle diameter changes occurred during 3 weeks. No significant incorporation was achieved for streptomycin, ethionamide, and rifampicin.

Six fungi strains (M. anisopliae 3935, 4516, 4819, PL57, PL43 and M. flavoviride CG291) were stud... more Six fungi strains (M. anisopliae 3935, 4516, 4819, PL57, PL43 and M. flavoviride CG291) were studied regarding their ability to produce an anticancer indolizidine alkaloid. The culture process was carried out in Shaken flask at 26ºC and 200 rpm using three different culture medium containing oat meal extract supplemented with glucose and DL-lysine or Czapek culture medium. The mycelial extracts

BACKGROUND: In this work recombinant gp-41 protein production by E. coli in an unconventional bio... more BACKGROUND: In this work recombinant gp-41 protein production by E. coli in an unconventional bioreactor coupled with a magnetic field generator was studied. The submerged fermentation process was carried out by fed-batch operation, recycling the culture medium externally through a stainless steel spiral U-shape tube inserted on the inside of a magnetic field generator. The exposure time and magnetic field induction were varied in a range from 1 to 12 h and 10 to 100 mT, respectively, according to a central composite design with centered face. RESULTS: It was found that the inhibitory or stimulatory effect of magnetic field on cell growth and protein production depended on exposure time and magnetic field strength. The production of recombinant gp41 protein was 20% higher than the control. CONCLUSION: The procedure here presented could be an easy-to-use approach to improve the efficiency of recombinant protein production by E. coli.

Liposomes have been extensively used as carriers of several compounds, due to their structural ve... more Liposomes have been extensively used as carriers of several compounds, due to their structural versatility in terms of size, composition, surface charge, lamellarity, and facility to incorporate hydrophilic and hydrophobic substances.

Biofuels in Brazil, 2014

ABSTRACT The use of renewable fuels, an alternative that reduces the generation of greenhouse gas... more ABSTRACT The use of renewable fuels, an alternative that reduces the generation of greenhouse gases, is one proposal to mitigate the effects that contribute to global warming. Brazil is the fourth largest producer of biodiesel in the world, and growth expectations of the productive capacities have generated huge technological and environmental challenges. In this context, this chapter discusses some of the relevant aspects of biodiesel production in Brazil, including sustainability of raw materials, conventional technology limitations, and further presents technological alternatives as strategies that will guide the future directions which can result in processes with greater environmental and economic returns.

Química Nova, 2009

Recebido em 28/4/08; aceito em 19/1/09; publicado na web em 28/7/09 EFFECT OF THE PRECURSOR ADDIT... more Recebido em 28/4/08; aceito em 19/1/09; publicado na web em 28/7/09 EFFECT OF THE PRECURSOR ADDITION ON THE ANTICANCER ALKALOID PRODUCTION USING EXPERIMENTAL DESIGN METHODOLOGY. The effect of precursors on the anticancer alkaloid production by submerged fermentation using M. anisopliae 3935 was studied, according to complete experimental design 2 2 with three central points. The results showed that lysine was the most important variable, however, when both lysine and glucose were added to the fermentation medium, the alkaloid production reached, approximately, 17 mg L -1 after 120 hours of fermentation. Then, the scale-up of the process was carried out and these results were confirmed. Finally, 35 mg L -1 of alkaloid at 192 h were attained after increment of added aminoacid lysine.

Process Biochemistry, 2006

The present study was designed to investigate the effect of extremely low frequency (ELF) magneti... more The present study was designed to investigate the effect of extremely low frequency (ELF) magnetic field (MF) on spinal cord injury (SCI)-induced osteoporosis in rats. Adult male Wistar rats (n = 24) were equally divided into sham, SCI, and SCI+MF groups. Complete transection of spinal cord (thoracic 11 vertebra) was surgically performed under anesthesia, whereas in the sham group only laminectomy was done. Post-SCI day 1, rats were either exposed (2 h/d × 8 wk) to ELF-MF (17.96 micro-Tesla, 50 Hz; SCI+MF group) or sham exposed (SCI group). Basso, Beattie, and Bresnahan (BBB) score was recorded weekly. All the rats were sacrificed 8 wk post-SCI; tibia and femur bones were isolated for the analysis of bone mineral content (BMC; total calcium [Ca], phosphorus [P], carbon [C]), bone mineral density (BMD), and biochemical status (osteocalcin, collagen I, alkaline phosphatase). The BBB score decreased post-SCI, which partially recovered after ELF-MF. In SCI rats, there was a statistically significant decrease in BMC, Ca, P, C, BMD, and biochemical level in both the bones as compared with the sham group, which was attenuated in SCI+MF rats except the C content. Electron microscopic study revealed the enhancement of microstructural composition and compactness in cortical and trabecular parts of treated bones. The results suggest that the chronic (2 h/d × 8 wk) ELF-MF exposure (17.96 micro-Tesla, 50 Hz) to SCI rats is effective in attenuating SCI-induced osteoporosis.

Applied Biochemistry and Biotechnology, 2006

The influence of extremely low-frequency (ELF) electromagnetic fields on Escherichia coli culture... more The influence of extremely low-frequency (ELF) electromagnetic fields on Escherichia coli cultures in submerse fermentation was studied. The fermentation processes were carried out recycling the culture medium externally through a stainless steel tube inserted in a magnetic field generator (solenoid). The exposure time and electromagnetic induction were varied in a range of 1 to 12 h and 0.010 to 0.10 T, respectively, according to a Box-Wilson Central Composite Designs of face centered with five central points. Growth of E. coli could be altered (stimulated or inhibited) under magnetic fieldinduced effects. E. coli cultures exposed at 0.1 T during 6.5 h exhibited changes in its viability compared to unexposed cells, which was 100 times higher than the control. The magnetic field generator associated with the cellular suspension recycle is a new way of magnetic treatment in fermentation processes and could be appropriate to industrial scale up.