Antonio Castilho | Universidade Estadual Paulista "Júlio de Mesquita Filho" (original) (raw)

Papers by Antonio Castilho

Nauplius

The aim of this study was to evaluate the gonadosomatic index (GSI) and the weight/length ratio i... more The aim of this study was to evaluate the gonadosomatic index (GSI) and the weight/length ratio in important shrimp populations (Xiphopenaeus kroyeri (Heller, 1862), Litopenaeus schmitti (Burkenroad, 1936), Rimapenaeus constrictus (Stimpson, 1874)) from the northern littoral of São Paulo State. The samples were collected between 2015 and 2016, with 212 females that were identified, classified according to their gonadal development stage, and measured (CL). The gonads were macroscopically classified into immature, spent and developed, and were then removed. Both body (BodW) and gonad (GonW) dry weights were obtained in order to calculate the gonadosomatic short communicAtion Nauplius, 28: e2020045

Zootaxa

This checklist is the third part of a series derived from a long-term multidisciplinary project o... more This checklist is the third part of a series derived from a long-term multidisciplinary project on the biodiversity of decapod crustaceans from marine and coastal environments of São Paulo state (Brazil). We integrated molecular techniques (DNA markers) and morphological analyses of adult specimens for accurate identifications. We compilated 185 species from the literature, but we confirmed the presence of 168 species: 130 of which we sampled, analyzed and obtained sequences (COI and/or 16S totalizing 113 sequences) and 38 that were not directly collected but were confirmed by analyses. In addition, 27 had their distribution removed from São Paulo due to uncertainties, and absence of material as voucher. Five species were reported for the first time on the coast of São Paulo (Calappa ocellata, Neohelice granulata, Teleophrys pococki, Teramnonotus monodi, Tetraxanthus rathbunae) and one on the Brazilian coast (Pseudomedaeus agassizi). Most of the non-sampled species previously report...

Nauplius

While previous studies on Alpheus formosus Gibbes, 1850 focused on taxonomic and phylogenetic iss... more While previous studies on Alpheus formosus Gibbes, 1850 focused on taxonomic and phylogenetic issues, the present study provides information on the biology and ecology of this snapping shrimp, a representative coral reef species widely distributed in the Atlantic Ocean. We studied A. formosus between 2015 and 2016 on two islands in São Paulo state, Brazil: Laje de Santos and Vitoria Island. We analyzed 47 specimens from Laje de Santos and 44 from Vitoria Island. Individuals ranged in size from 2.0 mm to 13.99 mm CL. The mean number of embryos attached to the pleopods of females was 211.28 ± 312.8 in Laje de Santos and 125.2 ± 177.8 in Vitoria Island. This species should be included in monitoring programs of coral ecosystems they inhabit as A. formosus represents a key element in trophic dynamics of these ecosystems.

Zootaxa

Among the Caridea Infraorder, the palaemonid shrimp from the genus Typton Costa, 1844 are commonl... more Among the Caridea Infraorder, the palaemonid shrimp from the genus Typton Costa, 1844 are commonly found in association with sponges, frequently feeding on the tissues of their hosts ((Ďuriš et al. 2011; Almeida et al. 2014; Pachelle et al. 2015; Soledade et al. 2017). Typton is mostly characterized by morphological features related to their sponge-dwelling lifestyle, as a simple and compressed rostrum, carapace smooth and antennal spines present, antennae extremely reduced and scaphocerite rudimentary. Mandible without palp, incisor process normal, reduced or absent. Second legs unequal, asymmetrical, without molar process on major chela (Bruce, 1972)

Nauplius

This study investigated the population dynamics of Persephona punctata with emphasis on populatio... more This study investigated the population dynamics of Persephona punctata with emphasis on population structure, sex-ratio, spatial distribution, maturity and reproductive period. Crabs and environmental factors (i.e., water temperature and salinity, and sediment texture and organic matter content) were collected monthly, from July 2012 to June 2014 in four sites within the Federal Environmental Protection Area of Cananéia-Iguape-Peruíbe: located in the oceanic area adjacent to the Cananéia region. The population had a female-biased sex-ratio. Persephona punctata had a seasonal reproduction and the higher percentage of ovigerous females (OF) occurred in spring and summer. There was a positive correlation ("lag 0") between temperature and number of OF (cross-correlation, p < 0.05) and a negative correlation between salinity and OF ("lag-2"), suggesting that periods of higher salinity may be favorable to larval hatching. The estimated size (carapace width-CW 50) at the onset of gonadal maturity of males (35.0 mm) was larger than that of females (30.1 mm), which is a common feature of Brachyura. Our results extend the knowledge on the life history of P. punctata and may be useful for the development of future mitigation measures aimed at its conservation.

Frontiers in Marine Science

EntomoBrasilis

Resumo. Besouros são insetos pertencentes à ordem Coleoptera, e compõem o grupo de animais mais d... more Resumo. Besouros são insetos pertencentes à ordem Coleoptera, e compõem o grupo de animais mais diverso do mundo. Esta ordem inclui a família Cerambycidae, composta pelos besouros serra-pau, com algumas espécies consideradas pragas para as monoculturas arbóreas. Nessa família é encontrado o besouro-escorpião Onychocerus albitarsis Pascoe, que possui um par de ferrões na extremidade das antenas, e que pode causar acidentes em humanos. A ocorrência desta espécie é pouco conhecida e registros no estado de São Paulo, Brasil, são pobremente documentados. Dois espécimes foram encontrados nesta região após causarem lesões em humanos, sendo coletados para identificação. Registramos a ocorrência de O. albitarsis no estado de São Paulo, e estas informações serão úteis para futuros estudos entomológicos, ecológicos e médicos sobre esse animal.Occurrence record of scorpion-beetle Onychocerus albitarsis Pascoe (Coleoptera: Cerambycidae) in São Paulo State, Brazil. Beetles are insects belonging t...

Invertebrate Reproduction & Development

We studied the shrimp Exhippolysmata oplophoroides in southeastern Brazil (Macaé, 22º37″S; Ubatub... more We studied the shrimp Exhippolysmata oplophoroides in southeastern Brazil (Macaé, 22º37″S; Ubatuba, 23º55″S; and Cananéia, 25º53″S) to test the hypotheses that (1) females should have the same reproductive output and fecundity at the regional scale, and (2) these reproductive parameters can vary among regions. Maximum carapace length (Macaé = 13.1 mm, Ubatuba = 12.8, Cananéia = 16), fecundity, and reproductive output differed among regions (Ancova, p < 0.05). Females in Cananéia exhibited the highest reproductive output/fecundity (18.6%/5230 embryos), followed by those in Macaé (16.0%/4365 embryos) and Ubatuba (12.6%/1206 embryos). We found that fecundity/reproductive output varies inversely with the length of the reproductive season, i.e. a considerable amount of energy is allocated during seasonal spawning periods in subtropical regions such as Cananéia. Previous studies have proved that E. oplophoroides is able to reproduce continuously in Ubatuba, with probably minor but continuous energy investment in reproduction occurring in this tropical region. In contrast, although Macaé is located at tropical latitude, it is under the influence of Cabo Frio upwelling that alters the water's physical properties via cold water intrusion. This process may be a determining factor in the spawning seasonality of Macaé's shrimp population, justifying the higher reproductive output recorded here than in Ubatuba.

Zootaxa, Jan 9, 2018

The current checklist is the result of a long-term multidisciplinary project which combined molec... more The current checklist is the result of a long-term multidisciplinary project which combined molecular techniques (mitochondrial DNA markers) and morphological analyses of adult specimens for an accurate and detailed identification of the total biodiversity of decapod crustaceans from marine and coastal (including estuaries) environments of São Paulo State (Brazil). This is the first of a series of reports and providing a checklist of caridean shrimps of the families Hippolytidae (5 spp.), Lysmatidae (6 spp.), Ogyrididae (2 spp.), Processidae (5 spp.) and Thoridae (1 sp.). We collected material of 13 species out of 19 recorded, with sequences of cytochrome oxidase subunit I - barcode region and 16S generated from 10 species. The previous record of Lysmata cf. intermedia for São Paulo is actually L. jundalini, as the first record in São Paulo/South Atlantic waters. The molecular data were helpful to confirm the identification of some species, as the occurrence of L. wurdemanni which i...

Nauplius

Th e goal of this study was to identify patt erns of shell occupation by diff erent species of he... more Th e goal of this study was to identify patt erns of shell occupation by diff erent species of hermit crabs from the southern Brazilian coast. In total, 644 individuals were collected, represented by six hermit species. Isocheles sawayai

Arthropod structure & development, Jan 27, 2017

We describe the sperm ultrastructure of six penaeid species, including at least one member of eac... more We describe the sperm ultrastructure of six penaeid species, including at least one member of each tribe (Penaeini, Parapenaeini and Trachypenaeini). Fragments of the vas deferens of the Penaeidae Farfantepenaeus brasiliensis, Farfantepenaeus paulensis, Litopenaeus schmitti, Parapenaeus americanus, Rimapenaeus constrictus and Xiphopenaeus kroyeri were fixed and processed according to the routine for transmission electron microscopy. The morphological results were contextualized in an evolutionary perspective using molecular markers for the phylogenetic reconstruction of this group. A phylogram was proposed by Bayesian inference based on 1007 bp of 33 sequences of the combined genes (16S rDNA and COI mtDNA) from 27 dendrobranchiate specimens. Our findings show that morphological differences in the sperm ultrastructures of members among the tribes of Penaeidae can be used as a baseline to understand their evolutionary relationships. Individuals from the Penaeini tribe show plesiomorph...

Invertebrate Reproduction & Development, 2017

Periclimenes paivai is notable for its symbiotic lifestyle with jellyfish. The objective of this ... more Periclimenes paivai is notable for its symbiotic lifestyle with jellyfish. The objective of this study was to investigate the fecundity and reproductive output of females, and specifically to determine whether females protected in their host have a greater reproductive success than other shrimp species. All anatomical parts of the medusae were analyzed and the P. paivai individuals were separated. Among 83 females analyzed, 65 presented embryos in the first developmental stage, and 18 were in the final stage of development. The mean fecundity was 229.08 ± 120.04 in initial-stage embryos and 191 ± 114.76 in final-stage embryos. We observed a positive relationship between the embryo number and the female size; however, the embryo number was not related to the embryonic stage, which indicates that females did not lose their embryos during the incubation period. The weight of brood mass during production is closely related to the female weight according to the results of linear regression, as fecundity increased with female size. In its symbiosis, P. paivai can increase the amount of energy used to produce embryos (mean reproductive output = 10.38%) and can elevate the reproductive success.

Invertebr Reprod Dev, 2011

The penaeidean Litopenaeus schmitti, popularly known as white shrimp, is a species of great econo... more The penaeidean Litopenaeus schmitti, popularly known as white shrimp, is a species of great economic importance, being a target of fishing fleets in the southeast region of Brazil. It is distributed through the western Atlantic, from Cuba to Brazil, until Rio Grande do Sul. Adults are found from shallow depths up to 30 m and have been found to depths of 47 m in the state of Rio de Janeiro, while juveniles are located in bays and estuaries. The studied species is seasonally distributed in the region of Ubatuba. The objectives of this study are to analyze the abundance and ecological distribution of L. schmitti and to assess if and when juveniles use the Indaia´estuary during their life cycle. Furthermore, the hypothesis was tested that the main period of recruitment in the bay coincides with the period of closure of fisheries defined by the Instruction of IBAMA. To that end, samples were taken monthly from July 2005 through June 2007, both in Ubatuba Bay and in the estuary formed by the Rio Indaia´. At each sampling station, salinity, temperature (bottom), depth, organic matter content (%), and grain size of sediments were measured. We found that the largest catches in the estuary were in late spring and early summer. In Ubatuba Bay, peak catches occurred during winter and early spring, whereas in the second year, already in May, there was a high peak capture. The variation in the number of individuals was correlated with some environmental factors both in the estuary and in the inlet (p 5 0.05). In the estuary, abundance was positively correlated with temperature (p ¼ 0.008) and organic matter (p ¼ 0.025) and negatively with depth (p ¼ 0.009). Regarding the Ubatuba Bay, only temperature (p ¼ 0.034) and depth (p ¼ 0.013) were significantly associated with the distribution of the shrimp, both being negative relations. The shrimp L. schmitti uses both the estuarine as well the shore environment, particularly the Ubatuba estuary and its adjacent bay, to complete its life cycle. The proposed period of fisheries closure (between March to May in the state of Sa˜o Paulo) for this and other shrimps coincides with individuals capable of reproduction entering the inlet and thus are being protected.

Zoomorphology, 2015

We describe the sperm ultrastructure of two sympatric rock shrimps, Sicyonia dorsalis and Sicyoni... more We describe the sperm ultrastructure of two sympatric rock shrimps, Sicyonia dorsalis and Sicyonia typica, and compare them in a molecular context to provide new insight into the sperm morphology of the Sicyoniidae. To accomplish this, the vasa deferentia of males of both species were fixed and prepared for transmission electron microscopy and light microscopy cytochemistry. Sperm of S. typica exhibit an oval main body and a short subacrosomal complex region with convoluted membrane pouches, a crystalline lattice and a well-developed large granule anterior to the spike, as previously described for Sicyonia carinata. In contrast, the ultrastructure of S. dorsalis sperm features an elongated complex subacrosomal region above the main body and a flat nucleus, and small cytoplasmic membrane vesicles are not present. Although S. dorsalis sperm are similar to those of Sicyonia ingentis, the spike of S. dorsalis is shorter than S. ingentis. A phylogenetic tree was built by Bayesian inference based on 19 sequences of 16S rDNA of some representatives of the genus. Our findings show that sperm analysis of these shrimps reflects their phylogenetic history and that such analysis is very useful for taxonomic studies. Species with corresponding sperm ultrastructure are in the same clade of the proposed phylogeny. Moreover, it is possible to detect different groups within the genus based on the presence of at least two distinct morphological patterns of ultrastructure in the spermatozoa of Sicyonia.

Anais da Academia Brasileira de Ciencias, Jan 27, 2015

The present study investigated the growth, longevity and reproductive dynamics ofArtemesia longin... more The present study investigated the growth, longevity and reproductive dynamics ofArtemesia longinaris in the southeastern coast of Brazil over a two-year period. Monthly collections were conducted in Ubatuba and Caraguatatuba using a shrimp fishing boat equipped with "double-rig" nets. Each region was divided into 7 sampling stations up to 35 m deep. Size frequency distributions, growth, longevity, sex ratio, and abundance of individuals in each demographic class, were compared. The relationship between abiotic factors and abundance of each demographic class was assessed using a Canonical Correlation Analysis. A total of 64,641 individuals were collected (6,928 measured) with an estimated longevity of 1.30 (Ubatuba) and 1.14 (Caraguatatuba) years for females and 1.03 years for males in both regions. There was a statistically significant bias in sex ratio toward females (Chi-squared test, p < 0.05) in both regions. The Canonical Correlation Analysis resulted in a canonic...

Gulf and Caribbean Research, 2007

The influence of environmental factors on the abundance and spatial-temporal distribution of the ... more The influence of environmental factors on the abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri was investigated in southeastern Brazil over 2 years. Monthly collections were conducted in Mar Virado, Ubatuba and Ubatumirim Bays using a commercial shrimp fishing boat equipped with 2 "double-rig" nets. Each bay was divided into 6 sampling stations, all of which were less than 25 m deep. The spatial distribution of X. kroyeri differed among Bays. Highest abundance values were recorded in areas where silt and clay comprised more than 70% of the bottom sediment. Abundance of X. kroyeri followed a seasonal trend, being higher during fall and winter, when intrusions of tropical waters are frequent, causing an increase in salinity (> 35‰) and temperature (> 21º C). A clear decrease in shrimp abundance followed a decrease in bottom temperature (< 20º C) during spring and s ummer due to the influence of cold water currents, particularly the South Atlantic Central Water. These results suggest that sediment type, salinity, and temperature are among the most important variables affecting the spatial and seasonal distribution of this species.

The influence of environmental factors on the abundance and spatial-temporal distribution of the ... more The influence of environmental factors on the abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri was investigated in southeastern Brazil over 2 years. Monthly collections were conducted in Mar Virado, Ubatuba and Ubatumirim Bays using a commercial shrimp fishing boat equipped with 2 "double-rig" nets. Each bay was divided into 6 sampling stations, all of which were less than 25 m deep. The spatial distribution of X. kroyeri differed among Bays. Highest abundance values were recorded in areas where silt and clay comprised more than 70% of the bottom sediment. Abundance of X. kroyeri followed a seasonal trend, being higher during fall and winter, when intrusions of tropical waters are frequent, causing an increase in salinity (> 35‰) and temperature (> 21º C). A clear decrease in shrimp abundance followed a decrease in bottom temperature (< 20º C) during spring and s ummer due to the influence of cold water currents, particularly the South Atlantic Central Water. These results suggest that sediment type, salinity, and temperature are among the most important variables affecting the spatial and seasonal distribution of this species.

Chinese Journal of Oceanology and Limnology, 2015

The predictability of certain environmental factors that affect the life cycle of the seabob shri... more The predictability of certain environmental factors that affect the life cycle of the seabob shrimp Xiphopenaeus kroyeri (Heller, 1862) was evaluated in a study of its reproductive biology in an area adjacent to Babitonga Bay, State of Santa Catarina, Brazil. Monthly sampling was conducted from July 2010 through June 2011 at depths of 5, 8, 11, 14, and 17 m. 76 004 individuals were obtained, with a pronounced peak in absolute abundance in austral autumn (34 208), coinciding with the annual closed season from March to May. Grain size composition of the sediment showed the closest relationship to the distribution of individuals (multiple linear regression, P <0.05), related to their burying habit. The observed correlations between the abundance of reproductive males (bearing spermatophores) and females with spent gonads (cross-correlation, P <0.05), and between reproductive males and reproductive females (with a 1-month lag) suggest that the peak of reproductive males preceded the peak of female ones. This result agrees with the pattern expected for females, which copulate in post-ecdysis (spent gonads). Spawning seemed to take place at greater depths, as evidenced by the concentration of reproductive females in these areas. The reproductive activities observed here confi rm that this species follows a tropical/subtropical reproductive pattern, spawning continuously throughout the year, with the highest peaks in spring and autumn. The data indicate that the juvenile recruitment period observed in August-September resulted from the reproductive output noted in April-May. Additionally, the reproductive period recorded in November led to the juvenile peak observed in March-May.

Aquatic Biology, 2012

The potential of a recently established marine protected area (MPA) in the Western Atlantic, Braz... more The potential of a recently established marine protected area (MPA) in the Western Atlantic, Brazil, as a 'seed production' and nursery ground for Xiphopenaeus kroyeri, an intensively exploited penaeid shrimp, was investigated in an attempt to reveal any future benefit of this new MPA to adjacent populations experiencing heavy exploitation. Overall, we observed that males and females >12 and 20 mm carapace length, respectively, contributed the most to reproduction in the studied population. Reproductive activity of X. kroyeri was continuous at the MPA; 2 annual reproductive peaks were recorded from March to April and from November to December, which were followed by recruitment events occurring from March to April 2009 and November 2009. Sediment, temperature, and algae and plant biomass floating near the bottom were relevant in driving reproductive activity and recruitment in X. kroyeri. The high reproductive potential of the studied population and the occurrence of abundant juveniles throughout the sampling area, indicating the existence of a nursery ground within the region, suggest that this MPA might provide important benefits in the near future. We argue in favor of future long-term studies on the larval dispersion, reproductive biology and ecology of X. kroyeri in MPAs and non-MPAs to construct a base for future management of this species and to aid stock recovery in fishing areas that are heavily exploited.

Nauplius

The aim of this study was to evaluate the gonadosomatic index (GSI) and the weight/length ratio i... more The aim of this study was to evaluate the gonadosomatic index (GSI) and the weight/length ratio in important shrimp populations (Xiphopenaeus kroyeri (Heller, 1862), Litopenaeus schmitti (Burkenroad, 1936), Rimapenaeus constrictus (Stimpson, 1874)) from the northern littoral of São Paulo State. The samples were collected between 2015 and 2016, with 212 females that were identified, classified according to their gonadal development stage, and measured (CL). The gonads were macroscopically classified into immature, spent and developed, and were then removed. Both body (BodW) and gonad (GonW) dry weights were obtained in order to calculate the gonadosomatic short communicAtion Nauplius, 28: e2020045

Zootaxa

This checklist is the third part of a series derived from a long-term multidisciplinary project o... more This checklist is the third part of a series derived from a long-term multidisciplinary project on the biodiversity of decapod crustaceans from marine and coastal environments of São Paulo state (Brazil). We integrated molecular techniques (DNA markers) and morphological analyses of adult specimens for accurate identifications. We compilated 185 species from the literature, but we confirmed the presence of 168 species: 130 of which we sampled, analyzed and obtained sequences (COI and/or 16S totalizing 113 sequences) and 38 that were not directly collected but were confirmed by analyses. In addition, 27 had their distribution removed from São Paulo due to uncertainties, and absence of material as voucher. Five species were reported for the first time on the coast of São Paulo (Calappa ocellata, Neohelice granulata, Teleophrys pococki, Teramnonotus monodi, Tetraxanthus rathbunae) and one on the Brazilian coast (Pseudomedaeus agassizi). Most of the non-sampled species previously report...

Nauplius

While previous studies on Alpheus formosus Gibbes, 1850 focused on taxonomic and phylogenetic iss... more While previous studies on Alpheus formosus Gibbes, 1850 focused on taxonomic and phylogenetic issues, the present study provides information on the biology and ecology of this snapping shrimp, a representative coral reef species widely distributed in the Atlantic Ocean. We studied A. formosus between 2015 and 2016 on two islands in São Paulo state, Brazil: Laje de Santos and Vitoria Island. We analyzed 47 specimens from Laje de Santos and 44 from Vitoria Island. Individuals ranged in size from 2.0 mm to 13.99 mm CL. The mean number of embryos attached to the pleopods of females was 211.28 ± 312.8 in Laje de Santos and 125.2 ± 177.8 in Vitoria Island. This species should be included in monitoring programs of coral ecosystems they inhabit as A. formosus represents a key element in trophic dynamics of these ecosystems.

Zootaxa

Among the Caridea Infraorder, the palaemonid shrimp from the genus Typton Costa, 1844 are commonl... more Among the Caridea Infraorder, the palaemonid shrimp from the genus Typton Costa, 1844 are commonly found in association with sponges, frequently feeding on the tissues of their hosts ((Ďuriš et al. 2011; Almeida et al. 2014; Pachelle et al. 2015; Soledade et al. 2017). Typton is mostly characterized by morphological features related to their sponge-dwelling lifestyle, as a simple and compressed rostrum, carapace smooth and antennal spines present, antennae extremely reduced and scaphocerite rudimentary. Mandible without palp, incisor process normal, reduced or absent. Second legs unequal, asymmetrical, without molar process on major chela (Bruce, 1972)

Nauplius

This study investigated the population dynamics of Persephona punctata with emphasis on populatio... more This study investigated the population dynamics of Persephona punctata with emphasis on population structure, sex-ratio, spatial distribution, maturity and reproductive period. Crabs and environmental factors (i.e., water temperature and salinity, and sediment texture and organic matter content) were collected monthly, from July 2012 to June 2014 in four sites within the Federal Environmental Protection Area of Cananéia-Iguape-Peruíbe: located in the oceanic area adjacent to the Cananéia region. The population had a female-biased sex-ratio. Persephona punctata had a seasonal reproduction and the higher percentage of ovigerous females (OF) occurred in spring and summer. There was a positive correlation ("lag 0") between temperature and number of OF (cross-correlation, p < 0.05) and a negative correlation between salinity and OF ("lag-2"), suggesting that periods of higher salinity may be favorable to larval hatching. The estimated size (carapace width-CW 50) at the onset of gonadal maturity of males (35.0 mm) was larger than that of females (30.1 mm), which is a common feature of Brachyura. Our results extend the knowledge on the life history of P. punctata and may be useful for the development of future mitigation measures aimed at its conservation.

Frontiers in Marine Science

EntomoBrasilis

Resumo. Besouros são insetos pertencentes à ordem Coleoptera, e compõem o grupo de animais mais d... more Resumo. Besouros são insetos pertencentes à ordem Coleoptera, e compõem o grupo de animais mais diverso do mundo. Esta ordem inclui a família Cerambycidae, composta pelos besouros serra-pau, com algumas espécies consideradas pragas para as monoculturas arbóreas. Nessa família é encontrado o besouro-escorpião Onychocerus albitarsis Pascoe, que possui um par de ferrões na extremidade das antenas, e que pode causar acidentes em humanos. A ocorrência desta espécie é pouco conhecida e registros no estado de São Paulo, Brasil, são pobremente documentados. Dois espécimes foram encontrados nesta região após causarem lesões em humanos, sendo coletados para identificação. Registramos a ocorrência de O. albitarsis no estado de São Paulo, e estas informações serão úteis para futuros estudos entomológicos, ecológicos e médicos sobre esse animal.Occurrence record of scorpion-beetle Onychocerus albitarsis Pascoe (Coleoptera: Cerambycidae) in São Paulo State, Brazil. Beetles are insects belonging t...

Invertebrate Reproduction & Development

We studied the shrimp Exhippolysmata oplophoroides in southeastern Brazil (Macaé, 22º37″S; Ubatub... more We studied the shrimp Exhippolysmata oplophoroides in southeastern Brazil (Macaé, 22º37″S; Ubatuba, 23º55″S; and Cananéia, 25º53″S) to test the hypotheses that (1) females should have the same reproductive output and fecundity at the regional scale, and (2) these reproductive parameters can vary among regions. Maximum carapace length (Macaé = 13.1 mm, Ubatuba = 12.8, Cananéia = 16), fecundity, and reproductive output differed among regions (Ancova, p < 0.05). Females in Cananéia exhibited the highest reproductive output/fecundity (18.6%/5230 embryos), followed by those in Macaé (16.0%/4365 embryos) and Ubatuba (12.6%/1206 embryos). We found that fecundity/reproductive output varies inversely with the length of the reproductive season, i.e. a considerable amount of energy is allocated during seasonal spawning periods in subtropical regions such as Cananéia. Previous studies have proved that E. oplophoroides is able to reproduce continuously in Ubatuba, with probably minor but continuous energy investment in reproduction occurring in this tropical region. In contrast, although Macaé is located at tropical latitude, it is under the influence of Cabo Frio upwelling that alters the water's physical properties via cold water intrusion. This process may be a determining factor in the spawning seasonality of Macaé's shrimp population, justifying the higher reproductive output recorded here than in Ubatuba.

Zootaxa, Jan 9, 2018

The current checklist is the result of a long-term multidisciplinary project which combined molec... more The current checklist is the result of a long-term multidisciplinary project which combined molecular techniques (mitochondrial DNA markers) and morphological analyses of adult specimens for an accurate and detailed identification of the total biodiversity of decapod crustaceans from marine and coastal (including estuaries) environments of São Paulo State (Brazil). This is the first of a series of reports and providing a checklist of caridean shrimps of the families Hippolytidae (5 spp.), Lysmatidae (6 spp.), Ogyrididae (2 spp.), Processidae (5 spp.) and Thoridae (1 sp.). We collected material of 13 species out of 19 recorded, with sequences of cytochrome oxidase subunit I - barcode region and 16S generated from 10 species. The previous record of Lysmata cf. intermedia for São Paulo is actually L. jundalini, as the first record in São Paulo/South Atlantic waters. The molecular data were helpful to confirm the identification of some species, as the occurrence of L. wurdemanni which i...

Nauplius

Th e goal of this study was to identify patt erns of shell occupation by diff erent species of he... more Th e goal of this study was to identify patt erns of shell occupation by diff erent species of hermit crabs from the southern Brazilian coast. In total, 644 individuals were collected, represented by six hermit species. Isocheles sawayai

Arthropod structure & development, Jan 27, 2017

We describe the sperm ultrastructure of six penaeid species, including at least one member of eac... more We describe the sperm ultrastructure of six penaeid species, including at least one member of each tribe (Penaeini, Parapenaeini and Trachypenaeini). Fragments of the vas deferens of the Penaeidae Farfantepenaeus brasiliensis, Farfantepenaeus paulensis, Litopenaeus schmitti, Parapenaeus americanus, Rimapenaeus constrictus and Xiphopenaeus kroyeri were fixed and processed according to the routine for transmission electron microscopy. The morphological results were contextualized in an evolutionary perspective using molecular markers for the phylogenetic reconstruction of this group. A phylogram was proposed by Bayesian inference based on 1007 bp of 33 sequences of the combined genes (16S rDNA and COI mtDNA) from 27 dendrobranchiate specimens. Our findings show that morphological differences in the sperm ultrastructures of members among the tribes of Penaeidae can be used as a baseline to understand their evolutionary relationships. Individuals from the Penaeini tribe show plesiomorph...

Invertebrate Reproduction & Development, 2017

Periclimenes paivai is notable for its symbiotic lifestyle with jellyfish. The objective of this ... more Periclimenes paivai is notable for its symbiotic lifestyle with jellyfish. The objective of this study was to investigate the fecundity and reproductive output of females, and specifically to determine whether females protected in their host have a greater reproductive success than other shrimp species. All anatomical parts of the medusae were analyzed and the P. paivai individuals were separated. Among 83 females analyzed, 65 presented embryos in the first developmental stage, and 18 were in the final stage of development. The mean fecundity was 229.08 ± 120.04 in initial-stage embryos and 191 ± 114.76 in final-stage embryos. We observed a positive relationship between the embryo number and the female size; however, the embryo number was not related to the embryonic stage, which indicates that females did not lose their embryos during the incubation period. The weight of brood mass during production is closely related to the female weight according to the results of linear regression, as fecundity increased with female size. In its symbiosis, P. paivai can increase the amount of energy used to produce embryos (mean reproductive output = 10.38%) and can elevate the reproductive success.

Invertebr Reprod Dev, 2011

The penaeidean Litopenaeus schmitti, popularly known as white shrimp, is a species of great econo... more The penaeidean Litopenaeus schmitti, popularly known as white shrimp, is a species of great economic importance, being a target of fishing fleets in the southeast region of Brazil. It is distributed through the western Atlantic, from Cuba to Brazil, until Rio Grande do Sul. Adults are found from shallow depths up to 30 m and have been found to depths of 47 m in the state of Rio de Janeiro, while juveniles are located in bays and estuaries. The studied species is seasonally distributed in the region of Ubatuba. The objectives of this study are to analyze the abundance and ecological distribution of L. schmitti and to assess if and when juveniles use the Indaia´estuary during their life cycle. Furthermore, the hypothesis was tested that the main period of recruitment in the bay coincides with the period of closure of fisheries defined by the Instruction of IBAMA. To that end, samples were taken monthly from July 2005 through June 2007, both in Ubatuba Bay and in the estuary formed by the Rio Indaia´. At each sampling station, salinity, temperature (bottom), depth, organic matter content (%), and grain size of sediments were measured. We found that the largest catches in the estuary were in late spring and early summer. In Ubatuba Bay, peak catches occurred during winter and early spring, whereas in the second year, already in May, there was a high peak capture. The variation in the number of individuals was correlated with some environmental factors both in the estuary and in the inlet (p 5 0.05). In the estuary, abundance was positively correlated with temperature (p ¼ 0.008) and organic matter (p ¼ 0.025) and negatively with depth (p ¼ 0.009). Regarding the Ubatuba Bay, only temperature (p ¼ 0.034) and depth (p ¼ 0.013) were significantly associated with the distribution of the shrimp, both being negative relations. The shrimp L. schmitti uses both the estuarine as well the shore environment, particularly the Ubatuba estuary and its adjacent bay, to complete its life cycle. The proposed period of fisheries closure (between March to May in the state of Sa˜o Paulo) for this and other shrimps coincides with individuals capable of reproduction entering the inlet and thus are being protected.

Zoomorphology, 2015

We describe the sperm ultrastructure of two sympatric rock shrimps, Sicyonia dorsalis and Sicyoni... more We describe the sperm ultrastructure of two sympatric rock shrimps, Sicyonia dorsalis and Sicyonia typica, and compare them in a molecular context to provide new insight into the sperm morphology of the Sicyoniidae. To accomplish this, the vasa deferentia of males of both species were fixed and prepared for transmission electron microscopy and light microscopy cytochemistry. Sperm of S. typica exhibit an oval main body and a short subacrosomal complex region with convoluted membrane pouches, a crystalline lattice and a well-developed large granule anterior to the spike, as previously described for Sicyonia carinata. In contrast, the ultrastructure of S. dorsalis sperm features an elongated complex subacrosomal region above the main body and a flat nucleus, and small cytoplasmic membrane vesicles are not present. Although S. dorsalis sperm are similar to those of Sicyonia ingentis, the spike of S. dorsalis is shorter than S. ingentis. A phylogenetic tree was built by Bayesian inference based on 19 sequences of 16S rDNA of some representatives of the genus. Our findings show that sperm analysis of these shrimps reflects their phylogenetic history and that such analysis is very useful for taxonomic studies. Species with corresponding sperm ultrastructure are in the same clade of the proposed phylogeny. Moreover, it is possible to detect different groups within the genus based on the presence of at least two distinct morphological patterns of ultrastructure in the spermatozoa of Sicyonia.

Anais da Academia Brasileira de Ciencias, Jan 27, 2015

The present study investigated the growth, longevity and reproductive dynamics ofArtemesia longin... more The present study investigated the growth, longevity and reproductive dynamics ofArtemesia longinaris in the southeastern coast of Brazil over a two-year period. Monthly collections were conducted in Ubatuba and Caraguatatuba using a shrimp fishing boat equipped with "double-rig" nets. Each region was divided into 7 sampling stations up to 35 m deep. Size frequency distributions, growth, longevity, sex ratio, and abundance of individuals in each demographic class, were compared. The relationship between abiotic factors and abundance of each demographic class was assessed using a Canonical Correlation Analysis. A total of 64,641 individuals were collected (6,928 measured) with an estimated longevity of 1.30 (Ubatuba) and 1.14 (Caraguatatuba) years for females and 1.03 years for males in both regions. There was a statistically significant bias in sex ratio toward females (Chi-squared test, p < 0.05) in both regions. The Canonical Correlation Analysis resulted in a canonic...

Gulf and Caribbean Research, 2007

The influence of environmental factors on the abundance and spatial-temporal distribution of the ... more The influence of environmental factors on the abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri was investigated in southeastern Brazil over 2 years. Monthly collections were conducted in Mar Virado, Ubatuba and Ubatumirim Bays using a commercial shrimp fishing boat equipped with 2 "double-rig" nets. Each bay was divided into 6 sampling stations, all of which were less than 25 m deep. The spatial distribution of X. kroyeri differed among Bays. Highest abundance values were recorded in areas where silt and clay comprised more than 70% of the bottom sediment. Abundance of X. kroyeri followed a seasonal trend, being higher during fall and winter, when intrusions of tropical waters are frequent, causing an increase in salinity (> 35‰) and temperature (> 21º C). A clear decrease in shrimp abundance followed a decrease in bottom temperature (< 20º C) during spring and s ummer due to the influence of cold water currents, particularly the South Atlantic Central Water. These results suggest that sediment type, salinity, and temperature are among the most important variables affecting the spatial and seasonal distribution of this species.

The influence of environmental factors on the abundance and spatial-temporal distribution of the ... more The influence of environmental factors on the abundance and spatial-temporal distribution of the shrimp Xiphopenaeus kroyeri was investigated in southeastern Brazil over 2 years. Monthly collections were conducted in Mar Virado, Ubatuba and Ubatumirim Bays using a commercial shrimp fishing boat equipped with 2 "double-rig" nets. Each bay was divided into 6 sampling stations, all of which were less than 25 m deep. The spatial distribution of X. kroyeri differed among Bays. Highest abundance values were recorded in areas where silt and clay comprised more than 70% of the bottom sediment. Abundance of X. kroyeri followed a seasonal trend, being higher during fall and winter, when intrusions of tropical waters are frequent, causing an increase in salinity (> 35‰) and temperature (> 21º C). A clear decrease in shrimp abundance followed a decrease in bottom temperature (< 20º C) during spring and s ummer due to the influence of cold water currents, particularly the South Atlantic Central Water. These results suggest that sediment type, salinity, and temperature are among the most important variables affecting the spatial and seasonal distribution of this species.

Chinese Journal of Oceanology and Limnology, 2015

The predictability of certain environmental factors that affect the life cycle of the seabob shri... more The predictability of certain environmental factors that affect the life cycle of the seabob shrimp Xiphopenaeus kroyeri (Heller, 1862) was evaluated in a study of its reproductive biology in an area adjacent to Babitonga Bay, State of Santa Catarina, Brazil. Monthly sampling was conducted from July 2010 through June 2011 at depths of 5, 8, 11, 14, and 17 m. 76 004 individuals were obtained, with a pronounced peak in absolute abundance in austral autumn (34 208), coinciding with the annual closed season from March to May. Grain size composition of the sediment showed the closest relationship to the distribution of individuals (multiple linear regression, P <0.05), related to their burying habit. The observed correlations between the abundance of reproductive males (bearing spermatophores) and females with spent gonads (cross-correlation, P <0.05), and between reproductive males and reproductive females (with a 1-month lag) suggest that the peak of reproductive males preceded the peak of female ones. This result agrees with the pattern expected for females, which copulate in post-ecdysis (spent gonads). Spawning seemed to take place at greater depths, as evidenced by the concentration of reproductive females in these areas. The reproductive activities observed here confi rm that this species follows a tropical/subtropical reproductive pattern, spawning continuously throughout the year, with the highest peaks in spring and autumn. The data indicate that the juvenile recruitment period observed in August-September resulted from the reproductive output noted in April-May. Additionally, the reproductive period recorded in November led to the juvenile peak observed in March-May.

Aquatic Biology, 2012

The potential of a recently established marine protected area (MPA) in the Western Atlantic, Braz... more The potential of a recently established marine protected area (MPA) in the Western Atlantic, Brazil, as a 'seed production' and nursery ground for Xiphopenaeus kroyeri, an intensively exploited penaeid shrimp, was investigated in an attempt to reveal any future benefit of this new MPA to adjacent populations experiencing heavy exploitation. Overall, we observed that males and females >12 and 20 mm carapace length, respectively, contributed the most to reproduction in the studied population. Reproductive activity of X. kroyeri was continuous at the MPA; 2 annual reproductive peaks were recorded from March to April and from November to December, which were followed by recruitment events occurring from March to April 2009 and November 2009. Sediment, temperature, and algae and plant biomass floating near the bottom were relevant in driving reproductive activity and recruitment in X. kroyeri. The high reproductive potential of the studied population and the occurrence of abundant juveniles throughout the sampling area, indicating the existence of a nursery ground within the region, suggest that this MPA might provide important benefits in the near future. We argue in favor of future long-term studies on the larval dispersion, reproductive biology and ecology of X. kroyeri in MPAs and non-MPAs to construct a base for future management of this species and to aid stock recovery in fishing areas that are heavily exploited.