Carlos Camargo | Universidade Estadual Paulista "Júlio de Mesquita Filho" (original) (raw)
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Objectives Besides possessing good mechanical properties, dental materials should present a good ... more Objectives Besides possessing good mechanical properties, dental materials should present a good biological behavior and should not injure the involved tissues. Bond strength and biocompatibility are both highly significant properties of dentin adhesives. For that matter, these properties of four generations of adhesive systems (Multi-Purpose/Single Bond/SE Plus/Easy Bond) were evaluated. Materials and methods Eighty bovine teeth had their dentin exposed (500-and 200-μm thickness). Adhesive was applied on the dentin layer of each specimen. Following that, the microshearing test was performed for all samples. A dentin barrier test was used for the cytotoxicity evaluation. Cell cultures (SV3NeoB) were collected from testing materials by means of 200-or 500-μm-thick dentin slices and placed in a cell culture perfusion chamber. Cell viability was measured 24 h post-exposition by means of a photometrical test (MTT test). Results The best bonding performance was shown by the single-step adhesive Easy Bond 21 MPa, 200 μm; 27 MPa, 500 μm) followed by Single Bond (15.6 MPa, 200 μm; 23.4 MPa, 500 μm), SE Plus (18.2 MPa, 200 μm; 20 MPa, 500 μm), and Multi-Purpose (15.2 MPa, 200 μm; 17.9 MPa, 500 μm). Regarding the cytotoxicity, Multi-Purpose slightly reduced the cell viability to 92 % (200 μm)/93 % (500 μm). Single Bond was reasonably cytotoxic, reducing cell viability to 71 % (200 μm)/64 % (500 μm). The self-etching adhesive Scotchbond SE decreased cell viability to 85 % (200 μm)/71 % (500 μm).
Objectives Besides possessing good mechanical properties, dental materials should present a good ... more Objectives Besides possessing good mechanical properties, dental materials should present a good biological behavior and should not injure the involved tissues. Bond strength and biocompatibility are both highly significant properties of dentin adhesives. For that matter, these properties of four generations of adhesive systems (Multi-Purpose/Single Bond/SE Plus/Easy Bond) were evaluated. Materials and methods Eighty bovine teeth had their dentin exposed (500-and 200-μm thickness). Adhesive was applied on the dentin layer of each specimen. Following that, the microshearing test was performed for all samples. A dentin barrier test was used for the cytotoxicity evaluation. Cell cultures (SV3NeoB) were collected from testing materials by means of 200-or 500-μm-thick dentin slices and placed in a cell culture perfusion chamber. Cell viability was measured 24 h post-exposition by means of a photometrical test (MTT test). Results The best bonding performance was shown by the single-step adhesive Easy Bond 21 MPa, 200 μm; 27 MPa, 500 μm) followed by Single Bond (15.6 MPa, 200 μm; 23.4 MPa, 500 μm), SE Plus (18.2 MPa, 200 μm; 20 MPa, 500 μm), and Multi-Purpose (15.2 MPa, 200 μm; 17.9 MPa, 500 μm). Regarding the cytotoxicity, Multi-Purpose slightly reduced the cell viability to 92 % (200 μm)/93 % (500 μm). Single Bond was reasonably cytotoxic, reducing cell viability to 71 % (200 μm)/64 % (500 μm). The self-etching adhesive Scotchbond SE decreased cell viability to 85 % (200 μm)/71 % (500 μm).