Cristina Antoniali | Universidade Estadual Paulista "Júlio de Mesquita Filho" (original) (raw)
Papers by Cristina Antoniali
Research Square (Research Square), Jul 7, 2023
Objective Isotretinoin, also known as 13-cis-retinoic acid, is an isomer of tretinoin, the oxidiz... more Objective Isotretinoin, also known as 13-cis-retinoic acid, is an isomer of tretinoin, the oxidized form of Vitamin A. Orthodontic tooth movement (OTM) is the result of a cascade of in ammatory responses stimulated by a physical element that is the force generated by orthodontic appliances. Considering that there are individuals undergoing orthodontic treatment while also using isotretinoin, and that there is limited information on the effects of this combination in the literature, we are proposing this study to evaluate the in uence of isotretinoin on OTM. Materials and Methods Thirty-ve animals were used, and they were randomly divided into 7 groups, containing 5 animals in each group. Group 1: Control; Group 2: OTM for 7 days; Group 3: OTM for 14 days; Group 4: Treated with isotretinoin for 14 days; Group 5: Treated with isotretinoin for 21 days; Group 6: Treated with isotretinoin for 14 days and undergoing OTM for 7 days; Group 7: Treated with isotretinoin for 21 days and undergoing OTM for 14 days. In Groups 6 and 7, the animals were treated with isotretinoin at a dosage of 1.0mg/kg/day for 7 days before OTM and maintained during the movement period in the respective groups. Results There was no signi cant difference on microtomographic parameters [Trabecular Volume (BV/TV), Trabecular Thickness (Tb.Th), Number of Trabeculae (Tb.N) and Trabecular Separation (Tb.Sp)] and tooth displacement between groups. Conclusion Isotretinoin did not cause a reduction in tooth displacement during OTM when administered at a dose of 1.0mg/kg/day and isotretinoin did not change the microtomographic parameters of animals treated with isotretinoin + OTM.
Europe PMC (PubMed Central), Jun 1, 2011
The FASEB Journal, Apr 1, 2019
Dental Traumatology, Aug 31, 2015
Systemic antibiotic therapy (SAT) has usually been recommended after tooth replantation, but its ... more Systemic antibiotic therapy (SAT) has usually been recommended after tooth replantation, but its actual value has been questioned. As there are no reports in the literature about its influence on tooth replantation, the aim of this study was to evaluate the influence of systemic administration of antibiotics (amoxicillin and tetracycline) at the different phases of the repair process (7, 15, 30 days) in delayed rat tooth replantation. Ninety Wistar rats (Rattus norvegicus albinus) had their maxillary right incisors extracted and bench-dried for 60 min. The dental papilla, enamel organ, pulp tissue, and root surface-adhered periodontal ligament were removed, and the teeth were replanted. The animals received no antibiotics (n = 30) or were medicated systemically with amoxicillin (n = 30) and tetracycline (n = 30), and were euthanized after 7, 15, and 30 days. Regardless of the evaluation period, the acute inflammatory infiltrate was less intense and root resorption presented smaller extent and depth in the group treated with amoxicillin. The results suggest that SAT has a positive influence on the repair process in delayed tooth replantation and that amoxicillin is an excellent treatment option.
Clinical Oral Investigations, Jul 24, 2015
The aim of this study was to evaluate the influence of systemic administration of antibiotics (am... more The aim of this study was to evaluate the influence of systemic administration of antibiotics (amoxicillin and tetracycline) at the different phases of the repair process (7, 15, 30 days) in immediate rat tooth replantation. Ninety rats had their incisors extracted and stored in saline for 5 min. Next, the teeth were replanted, and the animals were assigned to three groups according to the antibiotic administered by oral gavage: control group, amoxycillin group, and tetracycline group. Euthanasia was performed at 7, 15, and 30 days after replantation. Regardless of the evaluation period, the connective tissue underlying the epithelial attachment and the periodontal ligament showed statistically significant difference relative to the acute inflammatory infiltrate, which was more intense in the control group followed by the tetracycline group. These results point to the fact that systemic antibiotic therapy (SAT) in immediate tooth replantation is beneficial to pulpal and periodontal ligament repair and that amoxycillin is an excellent option. There is a lack of randomized studies assessing how the use of systemic antibiotics could influence tooth healing after immediate replantation.
Journal of Functional Biomaterials
Hypertension and estrogen deficiency can affect bone metabolism and therefore increase the risk o... more Hypertension and estrogen deficiency can affect bone metabolism and therefore increase the risk of osseointegration. Antihypertensive drugs such as losartan not only control blood pressure but also enhance bone healing. In addition, alendronate sodium is widely used to treat postmenopausal osteoporosis. Hence, we evaluated the effect of systemic antihypertensive and local alendronate coted on implants on osseointegration under hypertensive and estrogen-deficiency conditions. A total of 64 spontaneously hypertensive rats (SHRs) treated with losartan were randomly divided according to the estrogen-deficiency induction by ovariectomy (OVX) or not (SHAM), and whether the implant surface was coated with sodium alendronate (ALE) or not, resulting in four groups: SHR SHAM, SHR SHAM ALE, SHR OVX, and SHR OVX ALE. The removal torque, microcomputed tomography, and epifluorescence microscopy were the adopted analyses. The hypertensive and estrogen-deficiency animals presented a lower removal t...
Data in Brief, 2022
Oxidative stress is a key feature in hypertension, since reactive oxygen species are involved in ... more Oxidative stress is a key feature in hypertension, since reactive oxygen species are involved in all stages of cardiovascular diseases. Saliva is a body fluid that can be used to investigate alterations in the oxidative system with several specific advantages over blood. Nebivolol is a third-generation selective β1-adrenergic receptor antagonist that promotes vasodilation and has been shown to reduce oxidative stress in pre-clinical and clinical studies. The use of Nebivolol in different periods of treatment demonstrated that it is an efficient anti-hypertensive drug. We evaluated the oxidative stress biomarkers and the enzymatic and non-enzymatic antioxidant systems in saliva of hypertensive patients before and after the use of anti-hypertensive therapeutic doses of Nebivolol, since saliva can be used as an auxiliary tool to analyze parameters of oxidative stress.
Avaliamos o tratamento cronico com apocinina na Hipertrofia Ventricular de SHR. Wistar e SHR fora... more Avaliamos o tratamento cronico com apocinina na Hipertrofia Ventricular de SHR. Wistar e SHR foram tratados ou nao com apocinina (30 mg/Kg/dia, v.o., da 4a-10a semana de vida). Os animais foram sacrificados, coracao foi removido, depois o ventriculo esquerdo foi isolado e pesado, esses valores foram corrigidos em funcao do comprimento da tibia esquerda do animal, obtendo-se o Indice de Massa Ventricular Esquerda (PVE/comprimento da tibia esquerda, g/cm). Os ventriculos foram fixados em formalina tamp. 10% e preparados para realizacao dos cortes histologicos com coloracao Hematoxilina-Eosina ou Picrosirius Red. Avaliamos o diâmetro dos cardiomiocitos e a quantidade de colageno com o software Image J e os resultados expressos como media ± EPM dos valores obtidos, ANOVA (p<0.05). O IMVE foi semelhante entre SHR Tratados (0,13±0,07 g/cm, n=9) e Nao-Tratados (0,13±0,04 g/cm, n=11) e entre Wistar Tratados (0,13±0,06 g/cm, n=7) e Nao-Tratados (0,12±0,04 g/cm, n=12). A medida dos diâmetr...
Archives of Health Investigation, 2019
O principal fator de risco na sepse e a hipotensao causada por vasoplegia a vasoconstritores endo... more O principal fator de risco na sepse e a hipotensao causada por vasoplegia a vasoconstritores endogenos ou exogenos, que pode ser induzida por endotoxemia. Pesquisas recentes mostraram que a doenca periodontal (DP) promove alteracoes cardiovasculares prejudiciais, devido a liberacao de mediadores inflamatorios sistemicamente. A hipotese e que a DP promove vasoplegia a fenilefrina (PE) por aumentar a atividade da via PI3K-Akt-eNOS. O objetivo deste estudo foi avaliar se a DP altera a contracao a PE em aortas de ratos normotensos (Wistar) e espontaneamente hipertensos (SHR) e os mecanismos envolvidos nesta alteracao. Quatorze dias apos a inducao de DP em ratos Wistar e SHR, os animais foram eutanasiados e a aorta toracica foi removida e cortada em aneis. Os aneis com endotelio preservado ou removido foram conectados a um transdutor de tensao isometrica, mantidos em cuba para orgao isolado com solucao fisiologica e suprimento gasoso e estimulados com PE ou acetilcolina (ACh). Alguns ane...
Epidemiologia, diagnóstico e intervenções em odontologia, 2021
Research, Society and Development, 2021
Microcephaly is described as a reduction of the head circumference, due to the premature fusion o... more Microcephaly is described as a reduction of the head circumference, due to the premature fusion of the bones of the skull, preventing the brain from growing normally and reaching its maximum development. This condition may result in neurological disorders, phonation and chewing dysfunction, dysphagia and risk of malnutrition. This alteration contributes to oral hygiene impairment, and continuous uses of the antipsychotic and anticonvulsant medication. Thus, the purpose of this study was to evaluate if microcephaly modified redox balance in saliva. Our hypothesis is that in the microcephalic patient's salivary oxidative stress is lower because of the increase in antioxidant defenses. The study included 13 patients with microcephaly (microcephalic group – MC) and 12 patients without neurological disorders (normocephalic group – NC), from zero to ten years old, no edentulous. Saliva was collected using a cotton wool swab, placing it on the child's mouth floor. After centrifugat...
ARCHIVES OF HEALTH INVESTIGATION, 2018
8º Congresso de Odontologia de Araçatuba, 38º Jornada Acadêmica: Prof. Dr. Mauro Airton Rulli, 14... more 8º Congresso de Odontologia de Araçatuba, 38º Jornada Acadêmica: Prof. Dr. Mauro Airton Rulli, 14º Simpósio Pós-Graduação Profa. Dra. Kikue Takebayashi Sassaki, 10º Encontro do CAOE, Encontro de 40 anos de Egressos de Pós-Graduação em Odontologia (Área de C.T.B-M-F)
Biological trace element research, Jan 15, 2016
Osteoporosis is a systemic disease characterized by bone degradation and decreased bone mass that... more Osteoporosis is a systemic disease characterized by bone degradation and decreased bone mass that promotes increased bone fragility and eventual fracture risk. Studies have investigated the use of sodium fluoride (NaF) for the treatment of osteoporosis. However, fluoride can alter glucose homeostasis. The aim of this study was to evaluate the effects of NaF intake (50 mg/L) from water on the following parameters of ovariectomized (OVX) rats: (1) tyrosine phosphorylation status of insulin receptor substrate (pp185 (IRS-1/IRS-2)) in white adipose tissue; (2) insulin sensitivity; (3) plasma concentrations of glucose, insulin, total cholesterol, triglyceride, TNF-α, IL-6, osteocalcin, calcium, and fluoride; (4) bone density and biomechanical properties in the tibia; and (5) tibia histomorphometric analysis. Fifty-two Wistar rats (2 months old) were ovariectomized and distributed into two groups: control group (OVX-C) and NaF group (OVX-F), which was subjected to treatment with NaF (50 m...
Life sciences, Jan 5, 2016
The fetal programming hypothesis suggests that intrauterine stimuli can induce metabolic changes ... more The fetal programming hypothesis suggests that intrauterine stimuli can induce metabolic changes in offspring, increasing the disease risk in adulthood. Periodontal disease may enhance serum cytokine levels. Cytokines such as tumor necrosis factor-alpha (TNF-α) have been associated with reduced glucose transporter type 4 (GLUT4) expression, decreased protein kinase B (Akt) phosphorylation, and insulin resistance. This study aimed to evaluate GLUT4 content, and Akt serine phosphorylation status in the gastrocnemius skeletal muscle (GSM), glycemia, insulinemia and change in body weight in offspring of rats with periodontal disease. Female Wistar rats were distributed into a control group (CN) and an experimental periodontal disease group (PD), in which a ligature was placed around the mandibular first molars. Seven days after ligature placement, both groups were mated with normal male rats. The ligatures remained throughout pregnancy until weaning, after which the male offspring were ...
Research Square (Research Square), Jul 7, 2023
Objective Isotretinoin, also known as 13-cis-retinoic acid, is an isomer of tretinoin, the oxidiz... more Objective Isotretinoin, also known as 13-cis-retinoic acid, is an isomer of tretinoin, the oxidized form of Vitamin A. Orthodontic tooth movement (OTM) is the result of a cascade of in ammatory responses stimulated by a physical element that is the force generated by orthodontic appliances. Considering that there are individuals undergoing orthodontic treatment while also using isotretinoin, and that there is limited information on the effects of this combination in the literature, we are proposing this study to evaluate the in uence of isotretinoin on OTM. Materials and Methods Thirty-ve animals were used, and they were randomly divided into 7 groups, containing 5 animals in each group. Group 1: Control; Group 2: OTM for 7 days; Group 3: OTM for 14 days; Group 4: Treated with isotretinoin for 14 days; Group 5: Treated with isotretinoin for 21 days; Group 6: Treated with isotretinoin for 14 days and undergoing OTM for 7 days; Group 7: Treated with isotretinoin for 21 days and undergoing OTM for 14 days. In Groups 6 and 7, the animals were treated with isotretinoin at a dosage of 1.0mg/kg/day for 7 days before OTM and maintained during the movement period in the respective groups. Results There was no signi cant difference on microtomographic parameters [Trabecular Volume (BV/TV), Trabecular Thickness (Tb.Th), Number of Trabeculae (Tb.N) and Trabecular Separation (Tb.Sp)] and tooth displacement between groups. Conclusion Isotretinoin did not cause a reduction in tooth displacement during OTM when administered at a dose of 1.0mg/kg/day and isotretinoin did not change the microtomographic parameters of animals treated with isotretinoin + OTM.
Europe PMC (PubMed Central), Jun 1, 2011
The FASEB Journal, Apr 1, 2019
Dental Traumatology, Aug 31, 2015
Systemic antibiotic therapy (SAT) has usually been recommended after tooth replantation, but its ... more Systemic antibiotic therapy (SAT) has usually been recommended after tooth replantation, but its actual value has been questioned. As there are no reports in the literature about its influence on tooth replantation, the aim of this study was to evaluate the influence of systemic administration of antibiotics (amoxicillin and tetracycline) at the different phases of the repair process (7, 15, 30 days) in delayed rat tooth replantation. Ninety Wistar rats (Rattus norvegicus albinus) had their maxillary right incisors extracted and bench-dried for 60 min. The dental papilla, enamel organ, pulp tissue, and root surface-adhered periodontal ligament were removed, and the teeth were replanted. The animals received no antibiotics (n = 30) or were medicated systemically with amoxicillin (n = 30) and tetracycline (n = 30), and were euthanized after 7, 15, and 30 days. Regardless of the evaluation period, the acute inflammatory infiltrate was less intense and root resorption presented smaller extent and depth in the group treated with amoxicillin. The results suggest that SAT has a positive influence on the repair process in delayed tooth replantation and that amoxicillin is an excellent treatment option.
Clinical Oral Investigations, Jul 24, 2015
The aim of this study was to evaluate the influence of systemic administration of antibiotics (am... more The aim of this study was to evaluate the influence of systemic administration of antibiotics (amoxicillin and tetracycline) at the different phases of the repair process (7, 15, 30 days) in immediate rat tooth replantation. Ninety rats had their incisors extracted and stored in saline for 5 min. Next, the teeth were replanted, and the animals were assigned to three groups according to the antibiotic administered by oral gavage: control group, amoxycillin group, and tetracycline group. Euthanasia was performed at 7, 15, and 30 days after replantation. Regardless of the evaluation period, the connective tissue underlying the epithelial attachment and the periodontal ligament showed statistically significant difference relative to the acute inflammatory infiltrate, which was more intense in the control group followed by the tetracycline group. These results point to the fact that systemic antibiotic therapy (SAT) in immediate tooth replantation is beneficial to pulpal and periodontal ligament repair and that amoxycillin is an excellent option. There is a lack of randomized studies assessing how the use of systemic antibiotics could influence tooth healing after immediate replantation.
Journal of Functional Biomaterials
Hypertension and estrogen deficiency can affect bone metabolism and therefore increase the risk o... more Hypertension and estrogen deficiency can affect bone metabolism and therefore increase the risk of osseointegration. Antihypertensive drugs such as losartan not only control blood pressure but also enhance bone healing. In addition, alendronate sodium is widely used to treat postmenopausal osteoporosis. Hence, we evaluated the effect of systemic antihypertensive and local alendronate coted on implants on osseointegration under hypertensive and estrogen-deficiency conditions. A total of 64 spontaneously hypertensive rats (SHRs) treated with losartan were randomly divided according to the estrogen-deficiency induction by ovariectomy (OVX) or not (SHAM), and whether the implant surface was coated with sodium alendronate (ALE) or not, resulting in four groups: SHR SHAM, SHR SHAM ALE, SHR OVX, and SHR OVX ALE. The removal torque, microcomputed tomography, and epifluorescence microscopy were the adopted analyses. The hypertensive and estrogen-deficiency animals presented a lower removal t...
Data in Brief, 2022
Oxidative stress is a key feature in hypertension, since reactive oxygen species are involved in ... more Oxidative stress is a key feature in hypertension, since reactive oxygen species are involved in all stages of cardiovascular diseases. Saliva is a body fluid that can be used to investigate alterations in the oxidative system with several specific advantages over blood. Nebivolol is a third-generation selective β1-adrenergic receptor antagonist that promotes vasodilation and has been shown to reduce oxidative stress in pre-clinical and clinical studies. The use of Nebivolol in different periods of treatment demonstrated that it is an efficient anti-hypertensive drug. We evaluated the oxidative stress biomarkers and the enzymatic and non-enzymatic antioxidant systems in saliva of hypertensive patients before and after the use of anti-hypertensive therapeutic doses of Nebivolol, since saliva can be used as an auxiliary tool to analyze parameters of oxidative stress.
Avaliamos o tratamento cronico com apocinina na Hipertrofia Ventricular de SHR. Wistar e SHR fora... more Avaliamos o tratamento cronico com apocinina na Hipertrofia Ventricular de SHR. Wistar e SHR foram tratados ou nao com apocinina (30 mg/Kg/dia, v.o., da 4a-10a semana de vida). Os animais foram sacrificados, coracao foi removido, depois o ventriculo esquerdo foi isolado e pesado, esses valores foram corrigidos em funcao do comprimento da tibia esquerda do animal, obtendo-se o Indice de Massa Ventricular Esquerda (PVE/comprimento da tibia esquerda, g/cm). Os ventriculos foram fixados em formalina tamp. 10% e preparados para realizacao dos cortes histologicos com coloracao Hematoxilina-Eosina ou Picrosirius Red. Avaliamos o diâmetro dos cardiomiocitos e a quantidade de colageno com o software Image J e os resultados expressos como media ± EPM dos valores obtidos, ANOVA (p<0.05). O IMVE foi semelhante entre SHR Tratados (0,13±0,07 g/cm, n=9) e Nao-Tratados (0,13±0,04 g/cm, n=11) e entre Wistar Tratados (0,13±0,06 g/cm, n=7) e Nao-Tratados (0,12±0,04 g/cm, n=12). A medida dos diâmetr...
Archives of Health Investigation, 2019
O principal fator de risco na sepse e a hipotensao causada por vasoplegia a vasoconstritores endo... more O principal fator de risco na sepse e a hipotensao causada por vasoplegia a vasoconstritores endogenos ou exogenos, que pode ser induzida por endotoxemia. Pesquisas recentes mostraram que a doenca periodontal (DP) promove alteracoes cardiovasculares prejudiciais, devido a liberacao de mediadores inflamatorios sistemicamente. A hipotese e que a DP promove vasoplegia a fenilefrina (PE) por aumentar a atividade da via PI3K-Akt-eNOS. O objetivo deste estudo foi avaliar se a DP altera a contracao a PE em aortas de ratos normotensos (Wistar) e espontaneamente hipertensos (SHR) e os mecanismos envolvidos nesta alteracao. Quatorze dias apos a inducao de DP em ratos Wistar e SHR, os animais foram eutanasiados e a aorta toracica foi removida e cortada em aneis. Os aneis com endotelio preservado ou removido foram conectados a um transdutor de tensao isometrica, mantidos em cuba para orgao isolado com solucao fisiologica e suprimento gasoso e estimulados com PE ou acetilcolina (ACh). Alguns ane...
Epidemiologia, diagnóstico e intervenções em odontologia, 2021
Research, Society and Development, 2021
Microcephaly is described as a reduction of the head circumference, due to the premature fusion o... more Microcephaly is described as a reduction of the head circumference, due to the premature fusion of the bones of the skull, preventing the brain from growing normally and reaching its maximum development. This condition may result in neurological disorders, phonation and chewing dysfunction, dysphagia and risk of malnutrition. This alteration contributes to oral hygiene impairment, and continuous uses of the antipsychotic and anticonvulsant medication. Thus, the purpose of this study was to evaluate if microcephaly modified redox balance in saliva. Our hypothesis is that in the microcephalic patient's salivary oxidative stress is lower because of the increase in antioxidant defenses. The study included 13 patients with microcephaly (microcephalic group – MC) and 12 patients without neurological disorders (normocephalic group – NC), from zero to ten years old, no edentulous. Saliva was collected using a cotton wool swab, placing it on the child's mouth floor. After centrifugat...
ARCHIVES OF HEALTH INVESTIGATION, 2018
8º Congresso de Odontologia de Araçatuba, 38º Jornada Acadêmica: Prof. Dr. Mauro Airton Rulli, 14... more 8º Congresso de Odontologia de Araçatuba, 38º Jornada Acadêmica: Prof. Dr. Mauro Airton Rulli, 14º Simpósio Pós-Graduação Profa. Dra. Kikue Takebayashi Sassaki, 10º Encontro do CAOE, Encontro de 40 anos de Egressos de Pós-Graduação em Odontologia (Área de C.T.B-M-F)
Biological trace element research, Jan 15, 2016
Osteoporosis is a systemic disease characterized by bone degradation and decreased bone mass that... more Osteoporosis is a systemic disease characterized by bone degradation and decreased bone mass that promotes increased bone fragility and eventual fracture risk. Studies have investigated the use of sodium fluoride (NaF) for the treatment of osteoporosis. However, fluoride can alter glucose homeostasis. The aim of this study was to evaluate the effects of NaF intake (50 mg/L) from water on the following parameters of ovariectomized (OVX) rats: (1) tyrosine phosphorylation status of insulin receptor substrate (pp185 (IRS-1/IRS-2)) in white adipose tissue; (2) insulin sensitivity; (3) plasma concentrations of glucose, insulin, total cholesterol, triglyceride, TNF-α, IL-6, osteocalcin, calcium, and fluoride; (4) bone density and biomechanical properties in the tibia; and (5) tibia histomorphometric analysis. Fifty-two Wistar rats (2 months old) were ovariectomized and distributed into two groups: control group (OVX-C) and NaF group (OVX-F), which was subjected to treatment with NaF (50 m...
Life sciences, Jan 5, 2016
The fetal programming hypothesis suggests that intrauterine stimuli can induce metabolic changes ... more The fetal programming hypothesis suggests that intrauterine stimuli can induce metabolic changes in offspring, increasing the disease risk in adulthood. Periodontal disease may enhance serum cytokine levels. Cytokines such as tumor necrosis factor-alpha (TNF-α) have been associated with reduced glucose transporter type 4 (GLUT4) expression, decreased protein kinase B (Akt) phosphorylation, and insulin resistance. This study aimed to evaluate GLUT4 content, and Akt serine phosphorylation status in the gastrocnemius skeletal muscle (GSM), glycemia, insulinemia and change in body weight in offspring of rats with periodontal disease. Female Wistar rats were distributed into a control group (CN) and an experimental periodontal disease group (PD), in which a ligature was placed around the mandibular first molars. Seven days after ligature placement, both groups were mated with normal male rats. The ligatures remained throughout pregnancy until weaning, after which the male offspring were ...