Daniela Moris | Universidade Estadual Paulista "Júlio de Mesquita Filho" (original) (raw)
Papers by Daniela Moris
The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the id... more The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the identification of specific antibodies. The present study aimed to evaluate accuracy parameters of enzyme-linked immunosorbent assay (ELISA) and of the determination of serum galactomannan level in the diagnosis of patients with CPA, comparing these results with the double agar gel immunodiffusion (DID) test. In addition, the prevalence of cross-reactivity and the serological progression after treatment were evaluated by comparing DID and ELISA. Six study groups were formed: G1: 22 patients with CPA, 17 of whom had Aspergillus fungus ball, one chronic cavitary pulmonary aspergillosis (CCPA) and four chronic fibrosing pulmonary aspergillosis (CFPA); G2: 28 patients with pulmonary tubercu-losis (TB); G3: 23 patients with histoplasmosis (HST); G4: 50 patients with paracoccidioido-mycosis (PCM); G5: 20 patients with cryptococcosis (CRC); and G6: 200 healthy controls. Serum antibodies were measu...
The Brazilian Journal of Infectious Diseases, 2021
Journal of Pharmacy and Pharmacology, 2017
The genus Cryptococcus is composed of encapsulated yeasts that have the ability to infect and cau... more The genus Cryptococcus is composed of encapsulated yeasts that have the ability to infect and cause disease in humans, as Cryptococcus gattii/Cryptococcus neoformans species complex. Facing the current research panel, few studies about cryptococcosis and epidemiological data have been carried out in the western region of Sao Paulo state. This study aimed to verify the occurrence of Cryptococcus spp. In public areas of the city of Presidente Prudente, using pigeon droppings and tree hollows as an environmental source. The samples were identified by conventional mycological methods. Were collected 54 samples from pigeon droppings and 62 samples from tree hollows of the genus Licania. And of these samples, 47 (87.0%) and 14 (22.5%) had positive growth of yeast from pigeon droppings and tree hollows, respectively. Cryptococcus was identified in 11 (20.4%) of the samples from pigeon droppings and 3 (21.4%) of those from tree hollows. The following species were identified: Cryptococcus neoformans (7.15%), Cryptococcus laurentti (14.28%), and Cryptococcus albidus (78.57%). Decreased susceptibility to fluconazole was observed for some isolates. Fluconazole exhibited a limited in vitro activity, particularly against Cryptococcus albidus and Cryptococcus laurentii. Identification and susceptibility testing of Cryptococcus spp. should be performed on a routine basis in view of their unpredictable susceptibility profiles.
Brazilian Journal of Infectious Diseases, 2021
Brazilian Journal of Infectious Diseases, 2021
b r a z j i n f e c t d i s. 2 0 2 1;2 5(S 1):101078 esses antifúngicos. Foram observadas várias ... more b r a z j i n f e c t d i s. 2 0 2 1;2 5(S 1):101078 esses antifúngicos. Foram observadas várias Seç ões de Aspergillus no ar atmosférico, com prevalência de Nigri, e quase total ausência de isolados com potencial de resistência a antifúngicos de amplo uso clínico, incluindo Seç ão Fumigati, responsável por maior parte dos casos de aspergilose. No entanto, ocorrência de isolado da Seç ão Clavati, com provável mecanismo de resistência a posaconazol e alto valor de CIM para fungicida triazólico justifica o monitoramento de resistência ambiental para melhor compreensão dos quadros de aspergilose refratários à terapia azólica.
Revista Latino-americana de Ambiente Construído & Sustentabilidade, 2021
En Brasil, existe preocupación y cuidado por el entorno hospitalario y su planificación para la p... more En Brasil, existe preocupación y cuidado por el entorno hospitalario y su planificación para la preservación y prevención de enfermedades se expresa en los elementos arquitectónicos espaciales y formales del hospital. En este estudio, el objetivo fue comprender y discutir la funcionalidad del entorno hospitalario en el control de infecciones y la propagación de enfermedades. Se trata de un estudio cuantitativo-cualitativo que utiliza el aspecto técnico y funcional de una Evaluación Post-Ocupación (POE), a través de visitas in situ, levantamientos físicos y ambientales y evaluación de los ambientes hospitalarios docentes. La aplicación del instrumento permitió identificar ambientes con alto, medio y bajo potencial de crecimiento de microorganismos. El resultado de la investigación permitió verificar que las características arquitectónicas asociadas a las condiciones ambientales pueden favorecer el crecimiento de microorganismos, a pesar de que la principal forma de transmisión es a t...
Research, Society and Development, 2021
Hospital environment has factors that promote increased infections caused by microorganisms allow... more Hospital environment has factors that promote increased infections caused by microorganisms allowing them to remain viable for long periods on surfaces and fomites. The aim of our study was to identify the viability of Candida albicans in hospital environment common surfaces with biological fluids presence and disinfectants. Four surfaces (cotton fabric, synthetic fabric, egg crate foam mattress and, ceramic floor) were contaminated with a strain of Candida albicans ATCC 26790 and with an association of distilled water, blood, saliva and urine. Also, combinations of surfaces and fluids with addition of 70% alcohol and 2% sodium hypochlorite were tested. Viability was identified at 1, 3, 7, 14 and 21 days by Colony Forming Units counting. C. albicans presented 14 days viability in hospitals fabrics with absence of liquids and maintained this persistence under influence of distilled water and blood. It also showed low growth with urine addition and there was growth inhibition with 2% ...
Staphylococcus aureus is a bacterium that can cause a variety of infections. The great transmissi... more Staphylococcus aureus is a bacterium that can cause a variety of infections. The great transmissibility, its high pathogenic potential and the possibility of resistance to multiple antimicrobial agents, are relevant items that contribute to the staphylococcal infections. The present work aims to document the spread of isolates and to identify the phenotypic and genotypic factors of resistance evaluate the resistance of these strains of S. aureus isolated from nasal mucosa of medical students of an Educational Institution in the State of Sao Paulo, Brazil. Samples were collected from nasal fossae of fresh students of medicine using sterile swabs were submitted for the identification of S. aureus. Phenotypic resistance profile was performed with the technique of disk diffusion method and the genotipical detection of methicillin resistance was performed by the PCR technique to evaluating the presence of mecA gene. 222 nasal swab samples of fresh students of medicine were collected, 43 ...
Research, Society and Development, 2020
As infecções causadas por Cryptococus spp. são oportunistas, urbanas e associadas a imunossupress... more As infecções causadas por Cryptococus spp. são oportunistas, urbanas e associadas a imunossupressão. A mortalidade é estimada em 10% nos países desenvolvidos e 43% nos emergentes. O Objetivo foi avaliar os aspectos clínico-epidemiológicos da criptococose na região do oeste paulista. Trata-se de estudo transversal, baseado nas informações dos prontuários de pacientes em um hospital do interior de São Paulo, de 2006 a 2018. Aplicou-se análise descritiva por meio da frequência absoluta (n) e relativa (%); e estatística através dos testes x2 e Exato de Fisher. Também foi calculado Risco Relativo (RR) e Odds Ratio (OD) para óbito. A neurocriptococose foi a principal manifestação clínica em 64,50%. A pesquisa direta no líquor foi positiva em 85,71% das amostras, o imunodiagnóstico em 93,80% e a cultura, padrão-ouro para fungo, em 55,55% dos casos. O teste x2 e o Exato de Fisher não demonstraram diferença entre sexo, faixa etária, forma clínica ou óbito. RR e OD foram considerados baixos. ...
Memorias do Instituto Oswaldo Cruz, 2017
The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the ... more The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. To evaluate the distribution of whole blood T cell subsets, serum cytokines, and biomarkers of pulmonary fibrosis in PCM patients, according to the clinical form and at different time points, during the antifungal therapy. Eighty-seven PCM patients, from an endemic area in Brazil, were categorised into groups, according to the clinical form (AF or CF) and the moment of treatment. The peripheral blood T lymphocyte subsets of these patients were analysed using fluorescence-activated cell sorting. The serum levels of cytokines, basic fibroblast growth factor and surfactant protein-D (SP-D) were also analysed. In the CF patients, an expansion of the p...
Acta Scientiarum. Health Sciences, 2016
Staphylococcus aureus causes a large variety of infections, where many of them are acquired in th... more Staphylococcus aureus causes a large variety of infections, where many of them are acquired in the hospital environment. A significant part of the population is a nasal carrier of this type of microorganism. The present study evaluated the nasal colonization by S. aureus, identifying its resistance profile in nursing students from a private educational institute of higher education. Nasal swab samples were collected and identified for S. aureus. Moreover, an antibiogram assay was performed, followed by the search for ermA and ermC genes using PCR. Sixty-two students were included and we isolated 20 positive samples (32,5%) for S. aureus. For the phenotypic profile, 30% were found to be resistant to Erythromycin and 10% to Oxacillin and Cefoxitin. For the D-test in the genotypic profile, 25% presented mecA gene (MRSA), 5% of ermA gene, 35% of ermC gene and 10% with ermC and mecA genes. These data reinforce the necessity of monitoring bacterial colonization in hospital environment, which are potentially resistant in health professionals.
Anais da Academia Brasileira de Ciencias, Jan 15, 2016
Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pa... more Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures. From 204 valid samples, 20.6% (42 samples) were positive for S. aureus. For the assessment of phenotypic resistance by disk-diffusion technique, from 42 samples, 95.2% showed resistance to erythromycin, 42.8% to clindamycin, 16.6% to cephoxitin and 9.5% to oxacillin. The D test showed that 26.2% of samples were resistant to macrolides, lincosamides and streptogramin B. A PCR assay allowed for the evaluation of a genotypic resistance ...
COLLOQUIUM VITAE, 2015
A paracoccidioidomicose (PCM), micose sistemica causada pelo fungo termodimorfico Paracoccidioide... more A paracoccidioidomicose (PCM), micose sistemica causada pelo fungo termodimorfico Paracoccidioides brasiliensis afetando principalmente a populacao rural das Americas Latina e do Sul, sendo no Brasil e registrado 80% dos casos. A PCM e observada em pacientes que tiveram ou se encontram em contato direto e prolongado com o solo, como os trabalhadores rurais, sendo que as manifestacoes clinicas apresentam-se por infeccao ou doenca. A caracteristica principal da resposta imunologica da PCM e a formacao dos granulomas. Reacoes de imunodifusao dupla e os testes imunoenzimaticos sao amplamente utilizados e constituem uma alta importância no diagnostico de PCM. Sendo que o tratamento terapeutico deve ir alem da terapia antifungica convencional, e os pacientes devem ter acompanhamento medico constante ate o termino da doenca, cura. E apresentada aqui uma revisao da literatura atual concentrando-se na importância clinica, imunologia, metodos de diagnostico e tratamento da paracoccidioidomicose.
PloS one, 2015
The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the id... more The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the identification of specific antibodies. The present study aimed to evaluate accuracy parameters of enzyme-linked immunosorbent assay (ELISA) and of the determination of serum galactomannan level in the diagnosis of patients with CPA, comparing these results with the double agar gel immunodiffusion (DID) test. In addition, the prevalence of cross-reactivity and the serological progression after treatment were evaluated by comparing DID and ELISA. Six study groups were formed: G1: 22 patients with CPA, 17 of whom had Aspergillus fungus ball, one chronic cavitary pulmonary aspergillosis (CCPA) and four chronic fibrosing pulmonary aspergillosis (CFPA); G2: 28 patients with pulmonary tuberculosis (TB); G3: 23 patients with histoplasmosis (HST); G4: 50 patients with paracoccidioidomycosis (PCM); G5: 20 patients with cryptococcosis (CRC); and G6: 200 healthy controls. Serum antibodies were measure...
Candidiasis or thrush is a fungal infection (mycosis) of any of the Candida species. Also commonl... more Candidiasis or thrush is a fungal infection (mycosis) of any of the Candida species. Also commonly referred to as a yeast infection, candidiasis encompasses infections that range from superficial, such as oral thrush and vaginitis, to systemic and potentially life-threatening diseases. In this book, the authors discuss the epidemiology, symptoms and treatment options of candidiasis. Topics include invasive candidiasis and candida parapsilosis complex diagnoses and treatment; conventional and alternative treatment options for oral candidiasis; candida spp. in the oral cavity of children with immunodeficiencies; oxidative stress and the development of antifungal agents for the treatment of candidiasis; inhalation and topical steroid therapy and oral candidiasis; and fluorescent staining for the diagnosis of oral erythematous candidiasis. (Imprint: Nova Biomedical)
Future Microbiology, 2014
Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides. Materials & me... more Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides. Materials & methods: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination. Results: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity. Conclusion: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins. KEYWORDS • antigens • cryptococcosis • diagnosis • ELISA • synthetic peptides
Colloquium Vitae, 2012
RESUMO Staphylococcus aureus pode causar uma variedade de infecções, principalmente nosocomiais. ... more RESUMO Staphylococcus aureus pode causar uma variedade de infecções, principalmente nosocomiais. A sua importância reside na combinação da virulência, caráter invasivo e resistência aos antibióticos constituindo desafios terapêuticos. Este estudo objetivou avaliar fenotipica e genotipicamente o perfil de resistência de amostras de S. aureus isoladas de pacientes hospitalizados em um hospital de ensino brasileiro. Foram avaliadas 1078 amostras obtidas de culturas de vigilância e de amostras clínicas de S. aureus de pacientes internados. Para avaliação do perfil fenotípico de resistência foi utilizado o método de disco-difusão conforme os critérios do CLSI, 2011. Para a determinação genotípica de resistência foi avaliada a presença do gene mecA pela Reação de Polimerase em Cadeia-PCR. Das 1078 amostras testadas fenotipicamente, foi observado que 75,1% das amostras eram Staphylococcus aureus resistente a Meticilina (MRSA), sendo que destes, 98,4% apresentaram resistência a oxacilina e 100%, a cefoxitina. Para a determinação genotípica foi realizada a técnica de PCR para amplificação do gene mecA. Das 443 amostras testadas para o gene mecA foram positivas 336 amostras (75,8%). Destas amostras, 85,7% apresentaram fenótipo de resistência a oxacilina e 88,4% a cefoxitina. Devido ao alto índice de MRSA, conclui-se a necessidade de investimento em pesquisas, racionalização do uso de antimicrobianos e criação de laboratórios de referência para verificação de resistência aos antimicrobianos.
The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the id... more The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the identification of specific antibodies. The present study aimed to evaluate accuracy parameters of enzyme-linked immunosorbent assay (ELISA) and of the determination of serum galactomannan level in the diagnosis of patients with CPA, comparing these results with the double agar gel immunodiffusion (DID) test. In addition, the prevalence of cross-reactivity and the serological progression after treatment were evaluated by comparing DID and ELISA. Six study groups were formed: G1: 22 patients with CPA, 17 of whom had Aspergillus fungus ball, one chronic cavitary pulmonary aspergillosis (CCPA) and four chronic fibrosing pulmonary aspergillosis (CFPA); G2: 28 patients with pulmonary tubercu-losis (TB); G3: 23 patients with histoplasmosis (HST); G4: 50 patients with paracoccidioido-mycosis (PCM); G5: 20 patients with cryptococcosis (CRC); and G6: 200 healthy controls. Serum antibodies were measu...
The Brazilian Journal of Infectious Diseases, 2021
Journal of Pharmacy and Pharmacology, 2017
The genus Cryptococcus is composed of encapsulated yeasts that have the ability to infect and cau... more The genus Cryptococcus is composed of encapsulated yeasts that have the ability to infect and cause disease in humans, as Cryptococcus gattii/Cryptococcus neoformans species complex. Facing the current research panel, few studies about cryptococcosis and epidemiological data have been carried out in the western region of Sao Paulo state. This study aimed to verify the occurrence of Cryptococcus spp. In public areas of the city of Presidente Prudente, using pigeon droppings and tree hollows as an environmental source. The samples were identified by conventional mycological methods. Were collected 54 samples from pigeon droppings and 62 samples from tree hollows of the genus Licania. And of these samples, 47 (87.0%) and 14 (22.5%) had positive growth of yeast from pigeon droppings and tree hollows, respectively. Cryptococcus was identified in 11 (20.4%) of the samples from pigeon droppings and 3 (21.4%) of those from tree hollows. The following species were identified: Cryptococcus neoformans (7.15%), Cryptococcus laurentti (14.28%), and Cryptococcus albidus (78.57%). Decreased susceptibility to fluconazole was observed for some isolates. Fluconazole exhibited a limited in vitro activity, particularly against Cryptococcus albidus and Cryptococcus laurentii. Identification and susceptibility testing of Cryptococcus spp. should be performed on a routine basis in view of their unpredictable susceptibility profiles.
Brazilian Journal of Infectious Diseases, 2021
Brazilian Journal of Infectious Diseases, 2021
b r a z j i n f e c t d i s. 2 0 2 1;2 5(S 1):101078 esses antifúngicos. Foram observadas várias ... more b r a z j i n f e c t d i s. 2 0 2 1;2 5(S 1):101078 esses antifúngicos. Foram observadas várias Seç ões de Aspergillus no ar atmosférico, com prevalência de Nigri, e quase total ausência de isolados com potencial de resistência a antifúngicos de amplo uso clínico, incluindo Seç ão Fumigati, responsável por maior parte dos casos de aspergilose. No entanto, ocorrência de isolado da Seç ão Clavati, com provável mecanismo de resistência a posaconazol e alto valor de CIM para fungicida triazólico justifica o monitoramento de resistência ambiental para melhor compreensão dos quadros de aspergilose refratários à terapia azólica.
Revista Latino-americana de Ambiente Construído & Sustentabilidade, 2021
En Brasil, existe preocupación y cuidado por el entorno hospitalario y su planificación para la p... more En Brasil, existe preocupación y cuidado por el entorno hospitalario y su planificación para la preservación y prevención de enfermedades se expresa en los elementos arquitectónicos espaciales y formales del hospital. En este estudio, el objetivo fue comprender y discutir la funcionalidad del entorno hospitalario en el control de infecciones y la propagación de enfermedades. Se trata de un estudio cuantitativo-cualitativo que utiliza el aspecto técnico y funcional de una Evaluación Post-Ocupación (POE), a través de visitas in situ, levantamientos físicos y ambientales y evaluación de los ambientes hospitalarios docentes. La aplicación del instrumento permitió identificar ambientes con alto, medio y bajo potencial de crecimiento de microorganismos. El resultado de la investigación permitió verificar que las características arquitectónicas asociadas a las condiciones ambientales pueden favorecer el crecimiento de microorganismos, a pesar de que la principal forma de transmisión es a t...
Research, Society and Development, 2021
Hospital environment has factors that promote increased infections caused by microorganisms allow... more Hospital environment has factors that promote increased infections caused by microorganisms allowing them to remain viable for long periods on surfaces and fomites. The aim of our study was to identify the viability of Candida albicans in hospital environment common surfaces with biological fluids presence and disinfectants. Four surfaces (cotton fabric, synthetic fabric, egg crate foam mattress and, ceramic floor) were contaminated with a strain of Candida albicans ATCC 26790 and with an association of distilled water, blood, saliva and urine. Also, combinations of surfaces and fluids with addition of 70% alcohol and 2% sodium hypochlorite were tested. Viability was identified at 1, 3, 7, 14 and 21 days by Colony Forming Units counting. C. albicans presented 14 days viability in hospitals fabrics with absence of liquids and maintained this persistence under influence of distilled water and blood. It also showed low growth with urine addition and there was growth inhibition with 2% ...
Staphylococcus aureus is a bacterium that can cause a variety of infections. The great transmissi... more Staphylococcus aureus is a bacterium that can cause a variety of infections. The great transmissibility, its high pathogenic potential and the possibility of resistance to multiple antimicrobial agents, are relevant items that contribute to the staphylococcal infections. The present work aims to document the spread of isolates and to identify the phenotypic and genotypic factors of resistance evaluate the resistance of these strains of S. aureus isolated from nasal mucosa of medical students of an Educational Institution in the State of Sao Paulo, Brazil. Samples were collected from nasal fossae of fresh students of medicine using sterile swabs were submitted for the identification of S. aureus. Phenotypic resistance profile was performed with the technique of disk diffusion method and the genotipical detection of methicillin resistance was performed by the PCR technique to evaluating the presence of mecA gene. 222 nasal swab samples of fresh students of medicine were collected, 43 ...
Research, Society and Development, 2020
As infecções causadas por Cryptococus spp. são oportunistas, urbanas e associadas a imunossupress... more As infecções causadas por Cryptococus spp. são oportunistas, urbanas e associadas a imunossupressão. A mortalidade é estimada em 10% nos países desenvolvidos e 43% nos emergentes. O Objetivo foi avaliar os aspectos clínico-epidemiológicos da criptococose na região do oeste paulista. Trata-se de estudo transversal, baseado nas informações dos prontuários de pacientes em um hospital do interior de São Paulo, de 2006 a 2018. Aplicou-se análise descritiva por meio da frequência absoluta (n) e relativa (%); e estatística através dos testes x2 e Exato de Fisher. Também foi calculado Risco Relativo (RR) e Odds Ratio (OD) para óbito. A neurocriptococose foi a principal manifestação clínica em 64,50%. A pesquisa direta no líquor foi positiva em 85,71% das amostras, o imunodiagnóstico em 93,80% e a cultura, padrão-ouro para fungo, em 55,55% dos casos. O teste x2 e o Exato de Fisher não demonstraram diferença entre sexo, faixa etária, forma clínica ou óbito. RR e OD foram considerados baixos. ...
Memorias do Instituto Oswaldo Cruz, 2017
The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the ... more The main clinical forms of paracoccidioidomycosis (PCM) are the acute/subacute form (AF) and the chronic form (CF), and they both display considerable clinical variability. The immune responses of PCM patients, during and after treatment, remain neglected, mainly in the case of CF patients, due to the high prevalence of pulmonary sequelae. To evaluate the distribution of whole blood T cell subsets, serum cytokines, and biomarkers of pulmonary fibrosis in PCM patients, according to the clinical form and at different time points, during the antifungal therapy. Eighty-seven PCM patients, from an endemic area in Brazil, were categorised into groups, according to the clinical form (AF or CF) and the moment of treatment. The peripheral blood T lymphocyte subsets of these patients were analysed using fluorescence-activated cell sorting. The serum levels of cytokines, basic fibroblast growth factor and surfactant protein-D (SP-D) were also analysed. In the CF patients, an expansion of the p...
Acta Scientiarum. Health Sciences, 2016
Staphylococcus aureus causes a large variety of infections, where many of them are acquired in th... more Staphylococcus aureus causes a large variety of infections, where many of them are acquired in the hospital environment. A significant part of the population is a nasal carrier of this type of microorganism. The present study evaluated the nasal colonization by S. aureus, identifying its resistance profile in nursing students from a private educational institute of higher education. Nasal swab samples were collected and identified for S. aureus. Moreover, an antibiogram assay was performed, followed by the search for ermA and ermC genes using PCR. Sixty-two students were included and we isolated 20 positive samples (32,5%) for S. aureus. For the phenotypic profile, 30% were found to be resistant to Erythromycin and 10% to Oxacillin and Cefoxitin. For the D-test in the genotypic profile, 25% presented mecA gene (MRSA), 5% of ermA gene, 35% of ermC gene and 10% with ermC and mecA genes. These data reinforce the necessity of monitoring bacterial colonization in hospital environment, which are potentially resistant in health professionals.
Anais da Academia Brasileira de Ciencias, Jan 15, 2016
Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pa... more Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures. From 204 valid samples, 20.6% (42 samples) were positive for S. aureus. For the assessment of phenotypic resistance by disk-diffusion technique, from 42 samples, 95.2% showed resistance to erythromycin, 42.8% to clindamycin, 16.6% to cephoxitin and 9.5% to oxacillin. The D test showed that 26.2% of samples were resistant to macrolides, lincosamides and streptogramin B. A PCR assay allowed for the evaluation of a genotypic resistance ...
COLLOQUIUM VITAE, 2015
A paracoccidioidomicose (PCM), micose sistemica causada pelo fungo termodimorfico Paracoccidioide... more A paracoccidioidomicose (PCM), micose sistemica causada pelo fungo termodimorfico Paracoccidioides brasiliensis afetando principalmente a populacao rural das Americas Latina e do Sul, sendo no Brasil e registrado 80% dos casos. A PCM e observada em pacientes que tiveram ou se encontram em contato direto e prolongado com o solo, como os trabalhadores rurais, sendo que as manifestacoes clinicas apresentam-se por infeccao ou doenca. A caracteristica principal da resposta imunologica da PCM e a formacao dos granulomas. Reacoes de imunodifusao dupla e os testes imunoenzimaticos sao amplamente utilizados e constituem uma alta importância no diagnostico de PCM. Sendo que o tratamento terapeutico deve ir alem da terapia antifungica convencional, e os pacientes devem ter acompanhamento medico constante ate o termino da doenca, cura. E apresentada aqui uma revisao da literatura atual concentrando-se na importância clinica, imunologia, metodos de diagnostico e tratamento da paracoccidioidomicose.
PloS one, 2015
The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the id... more The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the identification of specific antibodies. The present study aimed to evaluate accuracy parameters of enzyme-linked immunosorbent assay (ELISA) and of the determination of serum galactomannan level in the diagnosis of patients with CPA, comparing these results with the double agar gel immunodiffusion (DID) test. In addition, the prevalence of cross-reactivity and the serological progression after treatment were evaluated by comparing DID and ELISA. Six study groups were formed: G1: 22 patients with CPA, 17 of whom had Aspergillus fungus ball, one chronic cavitary pulmonary aspergillosis (CCPA) and four chronic fibrosing pulmonary aspergillosis (CFPA); G2: 28 patients with pulmonary tuberculosis (TB); G3: 23 patients with histoplasmosis (HST); G4: 50 patients with paracoccidioidomycosis (PCM); G5: 20 patients with cryptococcosis (CRC); and G6: 200 healthy controls. Serum antibodies were measure...
Candidiasis or thrush is a fungal infection (mycosis) of any of the Candida species. Also commonl... more Candidiasis or thrush is a fungal infection (mycosis) of any of the Candida species. Also commonly referred to as a yeast infection, candidiasis encompasses infections that range from superficial, such as oral thrush and vaginitis, to systemic and potentially life-threatening diseases. In this book, the authors discuss the epidemiology, symptoms and treatment options of candidiasis. Topics include invasive candidiasis and candida parapsilosis complex diagnoses and treatment; conventional and alternative treatment options for oral candidiasis; candida spp. in the oral cavity of children with immunodeficiencies; oxidative stress and the development of antifungal agents for the treatment of candidiasis; inhalation and topical steroid therapy and oral candidiasis; and fluorescent staining for the diagnosis of oral erythematous candidiasis. (Imprint: Nova Biomedical)
Future Microbiology, 2014
Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides. Materials & me... more Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides. Materials & methods: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination. Results: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity. Conclusion: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins. KEYWORDS • antigens • cryptococcosis • diagnosis • ELISA • synthetic peptides
Colloquium Vitae, 2012
RESUMO Staphylococcus aureus pode causar uma variedade de infecções, principalmente nosocomiais. ... more RESUMO Staphylococcus aureus pode causar uma variedade de infecções, principalmente nosocomiais. A sua importância reside na combinação da virulência, caráter invasivo e resistência aos antibióticos constituindo desafios terapêuticos. Este estudo objetivou avaliar fenotipica e genotipicamente o perfil de resistência de amostras de S. aureus isoladas de pacientes hospitalizados em um hospital de ensino brasileiro. Foram avaliadas 1078 amostras obtidas de culturas de vigilância e de amostras clínicas de S. aureus de pacientes internados. Para avaliação do perfil fenotípico de resistência foi utilizado o método de disco-difusão conforme os critérios do CLSI, 2011. Para a determinação genotípica de resistência foi avaliada a presença do gene mecA pela Reação de Polimerase em Cadeia-PCR. Das 1078 amostras testadas fenotipicamente, foi observado que 75,1% das amostras eram Staphylococcus aureus resistente a Meticilina (MRSA), sendo que destes, 98,4% apresentaram resistência a oxacilina e 100%, a cefoxitina. Para a determinação genotípica foi realizada a técnica de PCR para amplificação do gene mecA. Das 443 amostras testadas para o gene mecA foram positivas 336 amostras (75,8%). Destas amostras, 85,7% apresentaram fenótipo de resistência a oxacilina e 88,4% a cefoxitina. Devido ao alto índice de MRSA, conclui-se a necessidade de investimento em pesquisas, racionalização do uso de antimicrobianos e criação de laboratórios de referência para verificação de resistência aos antimicrobianos.