Sueli Gardim | Universidade Estadual Paulista "Júlio de Mesquita Filho" (original) (raw)
Papers by Sueli Gardim
<i>Rhodnius montenegrensis</i> n. sp. <b>Holotype.</b> Ƥ, BRAZIL: Triatom... more <i>Rhodnius montenegrensis</i> n. sp. <b>Holotype.</b> Ƥ, BRAZIL: Triatominae Insectarium of the Faculty of Pharmaceutical Sciences, Unesp, Araraquara, SP, Rodovia Araraquara-Jaú km 1. 2012. <b>Paratypes.</b> BRAZIL: 8 Ƥ, 83, Oswaldo Cruz Institute, Rio de Janeiro, RJ, Avenida Brasil, 4365. 2012. <b>Etymology.</b> The name <i>Rhodnius montenegrensis</i> was chosen because this species was found in the municipality of Monte Negro, state of Rondônia, Brazil. <b>Description.</b> Length of male 18.49 ± 0.82 mm, of female 21.54 ± 0.75 mm, width of pronotum of male 3.99 ± 0.32 mm, of female 4.54 ± 0.26 mm; width of abdomen of male 6.16 ± 0.39 mm, of female 6.95 ± 0.39 mm (Table 1). <b>Female Male</b> <b>HL</b>, head length; <b>IE</b>, inner distance between eyes; <b>AO</b>, anteocular distance; <b>PO</b>, postocular distance (excluding neck); <b>DE</b>, diameter of the eye; <b>R 1</b>, <b>R 2,</b> and <b>R 3</b>, lengths of first, second, and third rostral segments, respectively; <b>TL</b>, Total length of the Triatominae; <b>MWT</b>, maximum width of the thorax; <b>MWA</b>, maximum width of the abdomen; <b>A 1</b>, <b>A 2</b>, <b>A 3</b> and <b>A4</b>, 1st, 2 nd, 3 rd, and 4 th antennal segments, respectively. The values in bold were significant at α = 0.05, using unpaired t-test. General color yellow with longitudinal dorsal black stripes on pronotum, wings, and connexivum (Fig. 2 A). Head of male and female with no spots, from clypeus to neck, with a central yellow stripe located between two (1 + 1) continuous brown stripes. Neck with a brown mark and a yellow central circular mark (Fig. 2 A). Head length, inner distance between eyes, anteocular distance, postocular distance (excluding neck), diameter of the eye, lengths of 1 st, 2 nd, and 3 rd -rostral segments, respectively, and 1 st, 2 nd, 3 rd, and 4 th -antennal segments, respectively, are shown in Table 1. Mesothoracic wings with well-demarcated veins, notably subcostal (Sc) vein with its yellow color (Fig. 4). Legs: yellow, except for a black stripe posterior [...]
FIGURE 16. Fingerprint of the triatominae species produced by PCR-RFLP with BstUI enzyme on 2% ag... more FIGURE 16. Fingerprint of the triatominae species produced by PCR-RFLP with BstUI enzyme on 2% agarose gel. M, 100bp DNA ladder marker; 1, R. montenegrensis n. sp.; 2, R. robustus.
FIGURE 15. Bayesian analyze based on Likelihood parameter among seven Rhodnius species and R. mon... more FIGURE 15. Bayesian analyze based on Likelihood parameter among seven Rhodnius species and R. montenegrensis n. sp. (CTA 088), based on Cyt b gene of the mtDNA sequences. Triatoma infestans and P. megistus were using by outgroup.
FIGURE 14. The factorial map of the wing shape for the specimens R. robustus and R. montenegrensi... more FIGURE 14. The factorial map of the wing shape for the specimens R. robustus and R. montenegrensis n. sp.
FIGURE 12. Egg general vision by scanning electron microscopy. A, R. montenegrensis n. sp.; B, R.... more FIGURE 12. Egg general vision by scanning electron microscopy. A, R. montenegrensis n. sp.; B, R. robustus. cl, collar; cr, chorial rim; ex, exochorion; nk, neck; op, operculum (Barata 1981).
FIGURE 11. Female external genitalia by SEM, posterior side. A, R. montenegrensis n. sp.; B, R. r... more FIGURE 11. Female external genitalia by SEM, posterior side. A, R. montenegrensis n. sp.; B, R. robustus. Ap, appendices; Gc 8, gonocoxite VIII; Gp 8, gonapophyse VIII; VII, VIII, IX, tergites; X, segment (Rosa et al. 2010).
FIGURE 10. Female external genitalia by SEM, dorsal side. A, R. montenegrensis n. sp.; B, R. robu... more FIGURE 10. Female external genitalia by SEM, dorsal side. A, R. montenegrensis n. sp.; B, R. robustus. VI, VII, VIII, IX, tergites (Rosa et al. 2010).
FIGURE 9. Female external genitalia by SEM, ventral side. A, R. montenegrensis n. sp., B, R. robu... more FIGURE 9. Female external genitalia by SEM, ventral side. A, R. montenegrensis n. sp., B, R. robustus. Gc 8, gonocoxite VIII; Gc 9, gonocoxite IX; Gp 8, gonapophyse VIII; VII, IX, esternites; X, segment (Rosa et al. 2010).
FIGURE 8. Median process of the pygophore by SEM. A, R. montenegrensis n. sp.; B, R. robustus. gc... more FIGURE 8. Median process of the pygophore by SEM. A, R. montenegrensis n. sp.; B, R. robustus. gc, groove cuticle; gp, gross point; nb, narrow triangular base; sc, smooth cuticle; sp, slender point; wb, wide triangular base.
FIGURE 7. Phallus of R. montenegrensis n. sp. (A, dorsal view; B, ventral view; C, lateral view) ... more FIGURE 7. Phallus of R. montenegrensis n. sp. (A, dorsal view; B, ventral view; C, lateral view) and R. robustus (D, dorsal view; E, ventral view; F, lateral view). Cj, conjunctive; En, endosome; EPlb, median extension of basal plate; P, phallus; Plb, basal plate; PrG, gonopore process; PrPh, phallosoma process; Ph, phallosoma; PrCj, conjunctive process.
FIGURE 6. Scutellum by SEM. A, R. montenegrensis n. sp.; B, R. robustus. pr, pronotum; sc, scutel... more FIGURE 6. Scutellum by SEM. A, R. montenegrensis n. sp.; B, R. robustus. pr, pronotum; sc, scutellum; sb, semi-circular base; sg, glabrous space; cd, central depression; le, lateral edge; ap, apex of scutellum; pu, process of the I urotergite; tg, transverse groove.
FIGURE 5. Process of the I urotergite. A, R. montenegrensis n. sp.; B, R. robustus. ap, apex of s... more FIGURE 5. Process of the I urotergite. A, R. montenegrensis n. sp.; B, R. robustus. ap, apex of scutellum; pu, process of the I urotergite; tg, transverse groove.
FIGURE 4. Wing of R. montenegrensis n. sp. with the seven landmarks used in morphometric analysis... more FIGURE 4. Wing of R. montenegrensis n. sp. with the seven landmarks used in morphometric analysis. According to Gurgel- Gonçalves et al. (2008). Sc, subcosta vein.
Revista de Ensino de Bioquímica, 2009
Abstract. The phylogenetic relationships among species of Triatoma matogrossensis subcomplex (T. ... more Abstract. The phylogenetic relationships among species of Triatoma matogrossensis subcomplex (T. baratai, T. guazu, T. matogrossensis, T. sordida, T. vandae, and T. williami) was addressed by using fragments of cytochrome oxidase I (COI), 16S rDNA (16S), and cytochrome b (Cytb) through Bayesian and parsimony analyses. We did not recover a monophyletic T. matogrossensis subcomplex, and their members were found clustered in
<i>Rhodnius montenegrensis</i> n. sp. <b>Holotype.</b> Ƥ, BRAZIL: Triatom... more <i>Rhodnius montenegrensis</i> n. sp. <b>Holotype.</b> Ƥ, BRAZIL: Triatominae Insectarium of the Faculty of Pharmaceutical Sciences, Unesp, Araraquara, SP, Rodovia Araraquara-Jaú km 1. 2012. <b>Paratypes.</b> BRAZIL: 8 Ƥ, 83, Oswaldo Cruz Institute, Rio de Janeiro, RJ, Avenida Brasil, 4365. 2012. <b>Etymology.</b> The name <i>Rhodnius montenegrensis</i> was chosen because this species was found in the municipality of Monte Negro, state of Rondônia, Brazil. <b>Description.</b> Length of male 18.49 ± 0.82 mm, of female 21.54 ± 0.75 mm, width of pronotum of male 3.99 ± 0.32 mm, of female 4.54 ± 0.26 mm; width of abdomen of male 6.16 ± 0.39 mm, of female 6.95 ± 0.39 mm (Table 1). <b>Female Male</b> <b>HL</b>, head length; <b>IE</b>, inner distance between eyes; <b>AO</b>, anteocular distance; <b>PO</b>, postocular distance (excluding neck); <b>DE</b>, diameter of the eye; <b>R 1</b>, <b>R 2,</b> and <b>R 3</b>, lengths of first, second, and third rostral segments, respectively; <b>TL</b>, Total length of the Triatominae; <b>MWT</b>, maximum width of the thorax; <b>MWA</b>, maximum width of the abdomen; <b>A 1</b>, <b>A 2</b>, <b>A 3</b> and <b>A4</b>, 1st, 2 nd, 3 rd, and 4 th antennal segments, respectively. The values in bold were significant at α = 0.05, using unpaired t-test. General color yellow with longitudinal dorsal black stripes on pronotum, wings, and connexivum (Fig. 2 A). Head of male and female with no spots, from clypeus to neck, with a central yellow stripe located between two (1 + 1) continuous brown stripes. Neck with a brown mark and a yellow central circular mark (Fig. 2 A). Head length, inner distance between eyes, anteocular distance, postocular distance (excluding neck), diameter of the eye, lengths of 1 st, 2 nd, and 3 rd -rostral segments, respectively, and 1 st, 2 nd, 3 rd, and 4 th -antennal segments, respectively, are shown in Table 1. Mesothoracic wings with well-demarcated veins, notably subcostal (Sc) vein with its yellow color (Fig. 4). Legs: yellow, except for a black stripe posterior [...]
FIGURE 16. Fingerprint of the triatominae species produced by PCR-RFLP with BstUI enzyme on 2% ag... more FIGURE 16. Fingerprint of the triatominae species produced by PCR-RFLP with BstUI enzyme on 2% agarose gel. M, 100bp DNA ladder marker; 1, R. montenegrensis n. sp.; 2, R. robustus.
FIGURE 15. Bayesian analyze based on Likelihood parameter among seven Rhodnius species and R. mon... more FIGURE 15. Bayesian analyze based on Likelihood parameter among seven Rhodnius species and R. montenegrensis n. sp. (CTA 088), based on Cyt b gene of the mtDNA sequences. Triatoma infestans and P. megistus were using by outgroup.
FIGURE 14. The factorial map of the wing shape for the specimens R. robustus and R. montenegrensi... more FIGURE 14. The factorial map of the wing shape for the specimens R. robustus and R. montenegrensis n. sp.
FIGURE 12. Egg general vision by scanning electron microscopy. A, R. montenegrensis n. sp.; B, R.... more FIGURE 12. Egg general vision by scanning electron microscopy. A, R. montenegrensis n. sp.; B, R. robustus. cl, collar; cr, chorial rim; ex, exochorion; nk, neck; op, operculum (Barata 1981).
FIGURE 11. Female external genitalia by SEM, posterior side. A, R. montenegrensis n. sp.; B, R. r... more FIGURE 11. Female external genitalia by SEM, posterior side. A, R. montenegrensis n. sp.; B, R. robustus. Ap, appendices; Gc 8, gonocoxite VIII; Gp 8, gonapophyse VIII; VII, VIII, IX, tergites; X, segment (Rosa et al. 2010).
FIGURE 10. Female external genitalia by SEM, dorsal side. A, R. montenegrensis n. sp.; B, R. robu... more FIGURE 10. Female external genitalia by SEM, dorsal side. A, R. montenegrensis n. sp.; B, R. robustus. VI, VII, VIII, IX, tergites (Rosa et al. 2010).
FIGURE 9. Female external genitalia by SEM, ventral side. A, R. montenegrensis n. sp., B, R. robu... more FIGURE 9. Female external genitalia by SEM, ventral side. A, R. montenegrensis n. sp., B, R. robustus. Gc 8, gonocoxite VIII; Gc 9, gonocoxite IX; Gp 8, gonapophyse VIII; VII, IX, esternites; X, segment (Rosa et al. 2010).
FIGURE 8. Median process of the pygophore by SEM. A, R. montenegrensis n. sp.; B, R. robustus. gc... more FIGURE 8. Median process of the pygophore by SEM. A, R. montenegrensis n. sp.; B, R. robustus. gc, groove cuticle; gp, gross point; nb, narrow triangular base; sc, smooth cuticle; sp, slender point; wb, wide triangular base.
FIGURE 7. Phallus of R. montenegrensis n. sp. (A, dorsal view; B, ventral view; C, lateral view) ... more FIGURE 7. Phallus of R. montenegrensis n. sp. (A, dorsal view; B, ventral view; C, lateral view) and R. robustus (D, dorsal view; E, ventral view; F, lateral view). Cj, conjunctive; En, endosome; EPlb, median extension of basal plate; P, phallus; Plb, basal plate; PrG, gonopore process; PrPh, phallosoma process; Ph, phallosoma; PrCj, conjunctive process.
FIGURE 6. Scutellum by SEM. A, R. montenegrensis n. sp.; B, R. robustus. pr, pronotum; sc, scutel... more FIGURE 6. Scutellum by SEM. A, R. montenegrensis n. sp.; B, R. robustus. pr, pronotum; sc, scutellum; sb, semi-circular base; sg, glabrous space; cd, central depression; le, lateral edge; ap, apex of scutellum; pu, process of the I urotergite; tg, transverse groove.
FIGURE 5. Process of the I urotergite. A, R. montenegrensis n. sp.; B, R. robustus. ap, apex of s... more FIGURE 5. Process of the I urotergite. A, R. montenegrensis n. sp.; B, R. robustus. ap, apex of scutellum; pu, process of the I urotergite; tg, transverse groove.
FIGURE 4. Wing of R. montenegrensis n. sp. with the seven landmarks used in morphometric analysis... more FIGURE 4. Wing of R. montenegrensis n. sp. with the seven landmarks used in morphometric analysis. According to Gurgel- Gonçalves et al. (2008). Sc, subcosta vein.
Revista de Ensino de Bioquímica, 2009
Abstract. The phylogenetic relationships among species of Triatoma matogrossensis subcomplex (T. ... more Abstract. The phylogenetic relationships among species of Triatoma matogrossensis subcomplex (T. baratai, T. guazu, T. matogrossensis, T. sordida, T. vandae, and T. williami) was addressed by using fragments of cytochrome oxidase I (COI), 16S rDNA (16S), and cytochrome b (Cytb) through Bayesian and parsimony analyses. We did not recover a monophyletic T. matogrossensis subcomplex, and their members were found clustered in