Bruno Streit | Goethe-Universität Frankfurt am Main (original) (raw)

Papers by Bruno Streit

BMC evolutionary biology, 2006

Reliable taxonomic identification at the species level is the basis for many biological disciplin... more Reliable taxonomic identification at the species level is the basis for many biological disciplines. In order to distinguish species, it is necessary that taxonomic characters allow for the separation of individuals into recognisable, homogeneous groups that differ from other such groups in a consistent way. We compared here the suitability and efficacy of traditionally used shell morphology and DNA-based methods to distinguish among species of the freshwater snail genus Radix (Basommatophora, Pulmonata). Morphometric analysis showed that shell shape was unsuitable to define homogeneous, recognisable entities, because the variation was continuous. On the other hand, the Molecularly defined Operational Taxonomic Units (MOTU), inferred from mitochondrial COI sequence variation, proved to be congruent with biological species, inferred from geographic distribution patterns, congruence with nuclear markers and crossing experiments. Moreover, it could be shown that the phenotypically plas...

EXS, 1994

In order to generate genetic markers from both nuclear and mitochondrial DNA, we used three PCR-b... more In order to generate genetic markers from both nuclear and mitochondrial DNA, we used three PCR-based techniques (RAPD, mtDNA-RFLPs, and sequencing of an amplified mtDNA fragment) to illuminate various aspects of the population genetics of large-lake Daphnia species. Estimations of genetic diversity at different taxonomic levels integrated with ecological data revealed insights into the genetic components of the evolutionary process of interspecific hybridization in these Daphnia species, which had previously been documented with allozyme markers. Our new molecular data suggest the occurrence of recent hybridization and backcrossing events, and allow the identification of the maternal species of hybrid clones.

... However, the factor time may be crucial: the last global warming event after the last glacial... more ... However, the factor time may be crucial: the last global warming event after the last glacial maximum took place over a much longer period, and the pace of recolonisation and range expansion was therefore less constrained. Currently, the cli-mate is projected to cha

BioInvasions Records, 2012

Ecotoxicology and Environmental Safety, 1998

The toxicity of lindane, hexachlorobenzene, and copper sulfate toTubifex tubifexandLimnodrilus ho... more The toxicity of lindane, hexachlorobenzene, and copper sulfate toTubifex tubifexandLimnodrilus hoffmeisteriwas determined using an easily applicable and standardizable 72-h short-term test system. It was designed for the quick assessment of sublethal and lethal effects of sediment-associated chemicals on the worms. An artificial sediment based on the Artificial Soil according to OECD Guideline No. 207 was used as test medium. The

Molecular Ecology Notes, 2006

Limnology and Oceanography, 2004

We studied genetic differentiation of two subarctic Daphnia species (subgenus Hyalodaphnia; Clado... more We studied genetic differentiation of two subarctic Daphnia species (subgenus Hyalodaphnia; Cladocera: An- omopoda) in relation to ecological and morphological diversification. Daphnia longispina and the recently discov- ered species Daphnia umbraare genetically differentiated based on mitochondrial 12S rDNA and internal transcribed spacer (ITS) regions. Genetic differentiation of 12S rDNA among the two sister taxa is in the range of differentiation

Molecular Ecology Notes, 2007

F1000Res, 2013

Male reproductive biology can by characterized through competition over mates as well as mate cho... more Male reproductive biology can by characterized through competition over mates as well as mate choice. Multiple mating and male mate choice copying, especially in internally fertilizing species, set the stage for increased sperm competition, i.e., sperm of two or more males can compete for fertilization of the female's ova. In the internally fertilizing fish Poecilia mexicana, males respond to the presence of rivals with reduced expression of mating preferences (audience effect), thereby lowering the risk of by-standing rivals copying their mate choice. Also, males interact initially more with a non-preferred female when observed by a rival, which has been interpreted in previous studies as a strategy to mislead rivals, again reducing sperm competition risk (SCR). Nevertheless, species might differ consistently in their expression of aggressive and reproductive behaviors, possibly due to varying levels of SCR. In the current study, we present a unique data set comprising ten poeciliid species (in two cases including multiple populations) and ask whether species can be characterized through consistent differences in the expression of aggression, sexual activity and changes in mate choice under increased SCR. We found consistent species-specific differences in aggressive behavior, sexual activity as well as in the level of misleading behavior, while decreased preference expression under increased SCR was a general feature of all but one species examined. Furthermore, mean sexual activity correlated positively with the occurrence of potentially misleading behavior. An alternative explanation for audience effects would be that males attempt to avoid aggressive encounters, which would predict stronger audience effects in more aggressive species. We demonstrate a positive correlation between mean aggressiveness and sexual activity (suggesting a hormonal link as a mechanistic explanation), but did not detect a correlation between aggressiveness and audience effects. Suites of correlated behavioral tendencies are termed behavioral syndromes, and our present study provides correlational evidence for the evolutionary significance of SCR in shaping a behavioral syndrome at the species level across poeciliid taxa.

Cancer Research, 2008

Abnormal activation of DNA repair pathways by deregulated signaling of receptor tyrosine kinase s... more Abnormal activation of DNA repair pathways by deregulated signaling of receptor tyrosine kinase systems is a compelling likelihood with significant implications in both cancer biology and treatment. Here, we show that due to a potential substrate switch, mutated variants of the receptor for hepatocyte growth factor Met, but not the wild-type form of the receptor, directly couple to the Abl tyrosine kinase and the Rad51 recombinase, two key signaling elements of homologous recombination-based DNA repair. Treatment of cells that express the mutated receptor variants with the Met inhibitor SU11274 leads, in a mutant-dependent manner, to a reduction of tyrosine phosphorylated levels of Abl and Rad51, impairs radiation-induced nuclear translocation of Rad51, and acts as a radiosensitizer together with the p53 inhibitor pifithrin-alpha by increasing cellular double-strand DNA break levels following exposure to ionizing radiation. Finally, we propose that in order to overcome a mutation-dependent resistance to SU11274, this aberrant molecular axis may alternatively be targeted with the Abl inhibitor, nilotinib.

Journal of Molecular Evolution, 2014

Patterns and processes of molecular evolution critically influence inferences in phylogeny and ph... more Patterns and processes of molecular evolution critically influence inferences in phylogeny and phylogeography. Within primates, a shift in evolutionary rates has been identified as the rationale for contrasting findings from mitochondrial and nuclear DNA studies as to the position of Tarsius. While the latter now seems settled, we sequenced complete mitochondrial genomes of three Sulawesi tarsiers (Tarsius dentatus, T. lariang, and T. wallacei) and analyzed substitution rates among tarsiers and other primates to infer driving processes of molecular evolution. We found substantial length polymorphism of the D-loop within tarsier individuals, but little variation of predominant lengths among them, regardless of species. Length variation was due to repetitive elements in the CSB domain-minisatellite motifs of 35 bp length and microsatellite motifs of 6 bp length. Amino acid evolutionary rates were second highest among major primate taxa relative to nucleotide substitution rates. We observed many radical possibly function-altering amino acid changes that were rarely driven by positive selection and thus potentially slightly deleterious or neutral. We hypothesize that the observed pattern of an increased amino acid evolutionary rate in tarsier mitochondrial genomes may be caused by hitchhiking of slightly deleterious mutations with favored D-loop length variants selected for maximizing replication success within the cell or the mitochondrion.

Zoological Journal of the Linnean Society, 2004

Convergent evolution of shell shape is a well known phenomenon in gastropods that has caused much... more Convergent evolution of shell shape is a well known phenomenon in gastropods that has caused much confusion in taxonomic and systematic studies. A paradigm is the patelliform shell shape in several taxa of the order Basommatophora. Historically, most freshwater limpets were ...

Zoologica Scripta, 2007

... Christian Albrecht,; Kerstin Kuhn,; Bruno Streit. ... del Rio 'Las Conchas&a... more ... Christian Albrecht,; Kerstin Kuhn,; Bruno Streit. ... del Rio 'Las Conchas', 20 m asl, 19.117°N; 96.115°W, 29 Mar 2002, R. Rojas, R. Rojas, EF012182/ EF012199, ... frequency with which the patelliform shell shape has evolved not only in freshwater limpets but also in marine snails might ...

ZooKeys, 2009

... Citation: Krupp F, Al-Jumaily M, Bariche M, Khalaf M, Malek M, Streit B (2009 ... We are part... more ... Citation: Krupp F, Al-Jumaily M, Bariche M, Khalaf M, Malek M, Streit B (2009 ... We are particularly grateful to the coordination assistants Nadia Man-asfi, Ilka Weidig and ... 1760, Article 2. WolfartR (1987) Late Cretaceous through Quaternary palaeogeographic evolution of the Mid ...

BMC evolutionary biology, 2006

Reliable taxonomic identification at the species level is the basis for many biological disciplin... more Reliable taxonomic identification at the species level is the basis for many biological disciplines. In order to distinguish species, it is necessary that taxonomic characters allow for the separation of individuals into recognisable, homogeneous groups that differ from other such groups in a consistent way. We compared here the suitability and efficacy of traditionally used shell morphology and DNA-based methods to distinguish among species of the freshwater snail genus Radix (Basommatophora, Pulmonata). Morphometric analysis showed that shell shape was unsuitable to define homogeneous, recognisable entities, because the variation was continuous. On the other hand, the Molecularly defined Operational Taxonomic Units (MOTU), inferred from mitochondrial COI sequence variation, proved to be congruent with biological species, inferred from geographic distribution patterns, congruence with nuclear markers and crossing experiments. Moreover, it could be shown that the phenotypically plas...

EXS, 1994

In order to generate genetic markers from both nuclear and mitochondrial DNA, we used three PCR-b... more In order to generate genetic markers from both nuclear and mitochondrial DNA, we used three PCR-based techniques (RAPD, mtDNA-RFLPs, and sequencing of an amplified mtDNA fragment) to illuminate various aspects of the population genetics of large-lake Daphnia species. Estimations of genetic diversity at different taxonomic levels integrated with ecological data revealed insights into the genetic components of the evolutionary process of interspecific hybridization in these Daphnia species, which had previously been documented with allozyme markers. Our new molecular data suggest the occurrence of recent hybridization and backcrossing events, and allow the identification of the maternal species of hybrid clones.

... However, the factor time may be crucial: the last global warming event after the last glacial... more ... However, the factor time may be crucial: the last global warming event after the last glacial maximum took place over a much longer period, and the pace of recolonisation and range expansion was therefore less constrained. Currently, the cli-mate is projected to cha

BioInvasions Records, 2012

Ecotoxicology and Environmental Safety, 1998

The toxicity of lindane, hexachlorobenzene, and copper sulfate toTubifex tubifexandLimnodrilus ho... more The toxicity of lindane, hexachlorobenzene, and copper sulfate toTubifex tubifexandLimnodrilus hoffmeisteriwas determined using an easily applicable and standardizable 72-h short-term test system. It was designed for the quick assessment of sublethal and lethal effects of sediment-associated chemicals on the worms. An artificial sediment based on the Artificial Soil according to OECD Guideline No. 207 was used as test medium. The

Molecular Ecology Notes, 2006

Limnology and Oceanography, 2004

We studied genetic differentiation of two subarctic Daphnia species (subgenus Hyalodaphnia; Clado... more We studied genetic differentiation of two subarctic Daphnia species (subgenus Hyalodaphnia; Cladocera: An- omopoda) in relation to ecological and morphological diversification. Daphnia longispina and the recently discov- ered species Daphnia umbraare genetically differentiated based on mitochondrial 12S rDNA and internal transcribed spacer (ITS) regions. Genetic differentiation of 12S rDNA among the two sister taxa is in the range of differentiation

Molecular Ecology Notes, 2007

F1000Res, 2013

Male reproductive biology can by characterized through competition over mates as well as mate cho... more Male reproductive biology can by characterized through competition over mates as well as mate choice. Multiple mating and male mate choice copying, especially in internally fertilizing species, set the stage for increased sperm competition, i.e., sperm of two or more males can compete for fertilization of the female's ova. In the internally fertilizing fish Poecilia mexicana, males respond to the presence of rivals with reduced expression of mating preferences (audience effect), thereby lowering the risk of by-standing rivals copying their mate choice. Also, males interact initially more with a non-preferred female when observed by a rival, which has been interpreted in previous studies as a strategy to mislead rivals, again reducing sperm competition risk (SCR). Nevertheless, species might differ consistently in their expression of aggressive and reproductive behaviors, possibly due to varying levels of SCR. In the current study, we present a unique data set comprising ten poeciliid species (in two cases including multiple populations) and ask whether species can be characterized through consistent differences in the expression of aggression, sexual activity and changes in mate choice under increased SCR. We found consistent species-specific differences in aggressive behavior, sexual activity as well as in the level of misleading behavior, while decreased preference expression under increased SCR was a general feature of all but one species examined. Furthermore, mean sexual activity correlated positively with the occurrence of potentially misleading behavior. An alternative explanation for audience effects would be that males attempt to avoid aggressive encounters, which would predict stronger audience effects in more aggressive species. We demonstrate a positive correlation between mean aggressiveness and sexual activity (suggesting a hormonal link as a mechanistic explanation), but did not detect a correlation between aggressiveness and audience effects. Suites of correlated behavioral tendencies are termed behavioral syndromes, and our present study provides correlational evidence for the evolutionary significance of SCR in shaping a behavioral syndrome at the species level across poeciliid taxa.

Cancer Research, 2008

Abnormal activation of DNA repair pathways by deregulated signaling of receptor tyrosine kinase s... more Abnormal activation of DNA repair pathways by deregulated signaling of receptor tyrosine kinase systems is a compelling likelihood with significant implications in both cancer biology and treatment. Here, we show that due to a potential substrate switch, mutated variants of the receptor for hepatocyte growth factor Met, but not the wild-type form of the receptor, directly couple to the Abl tyrosine kinase and the Rad51 recombinase, two key signaling elements of homologous recombination-based DNA repair. Treatment of cells that express the mutated receptor variants with the Met inhibitor SU11274 leads, in a mutant-dependent manner, to a reduction of tyrosine phosphorylated levels of Abl and Rad51, impairs radiation-induced nuclear translocation of Rad51, and acts as a radiosensitizer together with the p53 inhibitor pifithrin-alpha by increasing cellular double-strand DNA break levels following exposure to ionizing radiation. Finally, we propose that in order to overcome a mutation-dependent resistance to SU11274, this aberrant molecular axis may alternatively be targeted with the Abl inhibitor, nilotinib.

Journal of Molecular Evolution, 2014

Patterns and processes of molecular evolution critically influence inferences in phylogeny and ph... more Patterns and processes of molecular evolution critically influence inferences in phylogeny and phylogeography. Within primates, a shift in evolutionary rates has been identified as the rationale for contrasting findings from mitochondrial and nuclear DNA studies as to the position of Tarsius. While the latter now seems settled, we sequenced complete mitochondrial genomes of three Sulawesi tarsiers (Tarsius dentatus, T. lariang, and T. wallacei) and analyzed substitution rates among tarsiers and other primates to infer driving processes of molecular evolution. We found substantial length polymorphism of the D-loop within tarsier individuals, but little variation of predominant lengths among them, regardless of species. Length variation was due to repetitive elements in the CSB domain-minisatellite motifs of 35 bp length and microsatellite motifs of 6 bp length. Amino acid evolutionary rates were second highest among major primate taxa relative to nucleotide substitution rates. We observed many radical possibly function-altering amino acid changes that were rarely driven by positive selection and thus potentially slightly deleterious or neutral. We hypothesize that the observed pattern of an increased amino acid evolutionary rate in tarsier mitochondrial genomes may be caused by hitchhiking of slightly deleterious mutations with favored D-loop length variants selected for maximizing replication success within the cell or the mitochondrion.

Zoological Journal of the Linnean Society, 2004

Convergent evolution of shell shape is a well known phenomenon in gastropods that has caused much... more Convergent evolution of shell shape is a well known phenomenon in gastropods that has caused much confusion in taxonomic and systematic studies. A paradigm is the patelliform shell shape in several taxa of the order Basommatophora. Historically, most freshwater limpets were ...

Zoologica Scripta, 2007

... Christian Albrecht,; Kerstin Kuhn,; Bruno Streit. ... del Rio 'Las Conchas&a... more ... Christian Albrecht,; Kerstin Kuhn,; Bruno Streit. ... del Rio 'Las Conchas', 20 m asl, 19.117°N; 96.115°W, 29 Mar 2002, R. Rojas, R. Rojas, EF012182/ EF012199, ... frequency with which the patelliform shell shape has evolved not only in freshwater limpets but also in marine snails might ...

ZooKeys, 2009

... Citation: Krupp F, Al-Jumaily M, Bariche M, Khalaf M, Malek M, Streit B (2009 ... We are part... more ... Citation: Krupp F, Al-Jumaily M, Bariche M, Khalaf M, Malek M, Streit B (2009 ... We are particularly grateful to the coordination assistants Nadia Man-asfi, Ilka Weidig and ... 1760, Article 2. WolfartR (1987) Late Cretaceous through Quaternary palaeogeographic evolution of the Mid ...