Tuấn Nghĩa Lê - Profile on Academia.edu (original) (raw)
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Methods: 1. Prepare the reaction of: Plasmid-DNA 30 µl Eco R I (10U/µl) 4,0 µl Hind III (10U/µl) ... more Methods: 1. Prepare the reaction of: Plasmid-DNA 30 µl Eco R I (10U/µl) 4,0 µl Hind III (10U/µl) 2,0 µl Buffer R+ (10x) 5,0 µl H 2 O 9,0 µl 50 µl 2. Incubate the reaction for 1 h at 37 0 C 3. Add 10 µl of loading-buffer 4. Load the reaction on 1,0 % agarose gel supplemented with 25 µl of Gel Green.
Methods: 1. Prepare the reaction of: Plasmid-DNA 30 µl Eco R I (10U/µl) 4,0 µl Hind III (10U/µl) ... more Methods: 1. Prepare the reaction of: Plasmid-DNA 30 µl Eco R I (10U/µl) 4,0 µl Hind III (10U/µl) 2,0 µl Buffer R+ (10x) 5,0 µl H 2 O 9,0 µl 50 µl 2. Incubate the reaction for 1 h at 37 0 C 3. Add 10 µl of loading-buffer 4. Load the reaction on 1,0 % agarose gel supplemented with 25 µl of Gel Green.