Anand S | University of Mysore (original) (raw)
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The work was carried out to develop an immunoassay for estimation of Aspergillus ochraceus biomas... more The work was carried out to develop an immunoassay for estimation of Aspergillus ochraceus biomass on solid substrate. Methods and Results: An indirect noncompetitive enzyme-linked immunosorbent assay (ELISA) was developed for determination of fungal biomass in food commodities using antibody raised against A. ochraceus mycelial antigen. The sensitivity of the assay was linear in the range of 10-160 lg fungal biomass per millilitre extract of coffee (R 2 = 0AE989), poultry feed (R 2 = 0AE987) and chilli (R 2 = 0AE989). The growth of A. ochraceus in the food commodities like chilli, coffee beans and poultry feed, under the influence of two levels of moisture (20% and 30%) were monitored by the ELISA. The maximum fungal colonization was observed in poultry feed (9AE8 and 11AE8 mg g )1 ) followed by coffee beans (6AE8 and 11AE3 mg g )1 ) and chilli (5AE1 and 6AE3 mg g )1 ) at 20% and 30% moisture after 20 days of incubation. Similarly the fungus produced maximum ochratoxin A in poultry feed (25 and 120 lg g )1 ) followed by coffee beans (8 and 24 lg g )1 ) and chilli (0AE2 and 0AE45 lg g )1 ) at 20% and 30% moisture after 20 days of incubation. Conclusions: The method can be used for quantitative estimation of fungal biomass and comparison of fungal colonization in food substrates varying in composition. Significance and Impact of the Study: The method can be adapted for studying the fungal colonization in different solid substrates under different culture condition. The method is sensitive to mould colonization of ‡0AE02% (w/w) and can be used for early detection of specific fungal infestation in food commodities.
The work was carried out to develop an immunoassay for estimation of Aspergillus ochraceus biomas... more The work was carried out to develop an immunoassay for estimation of Aspergillus ochraceus biomass on solid substrate. Methods and Results: An indirect noncompetitive enzyme-linked immunosorbent assay (ELISA) was developed for determination of fungal biomass in food commodities using antibody raised against A. ochraceus mycelial antigen. The sensitivity of the assay was linear in the range of 10-160 lg fungal biomass per millilitre extract of coffee (R 2 = 0AE989), poultry feed (R 2 = 0AE987) and chilli (R 2 = 0AE989). The growth of A. ochraceus in the food commodities like chilli, coffee beans and poultry feed, under the influence of two levels of moisture (20% and 30%) were monitored by the ELISA. The maximum fungal colonization was observed in poultry feed (9AE8 and 11AE8 mg g )1 ) followed by coffee beans (6AE8 and 11AE3 mg g )1 ) and chilli (5AE1 and 6AE3 mg g )1 ) at 20% and 30% moisture after 20 days of incubation. Similarly the fungus produced maximum ochratoxin A in poultry feed (25 and 120 lg g )1 ) followed by coffee beans (8 and 24 lg g )1 ) and chilli (0AE2 and 0AE45 lg g )1 ) at 20% and 30% moisture after 20 days of incubation. Conclusions: The method can be used for quantitative estimation of fungal biomass and comparison of fungal colonization in food substrates varying in composition. Significance and Impact of the Study: The method can be adapted for studying the fungal colonization in different solid substrates under different culture condition. The method is sensitive to mould colonization of ‡0AE02% (w/w) and can be used for early detection of specific fungal infestation in food commodities.