Jens Rettig | Saarland University (original) (raw)
Papers by Jens Rettig
Scientific Reports, 2016
A/B toxins such as cholera toxin, Pseudomonas exotoxin and killer toxin K28 contain a KDEL-like a... more A/B toxins such as cholera toxin, Pseudomonas exotoxin and killer toxin K28 contain a KDEL-like amino acid motif at one of their subunits which ensures retrograde toxin transport through the secretory pathway of a target cell. As key step in host cell invasion, each toxin binds to distinct plasma membrane receptors that are utilized for cell entry. Despite intensive efforts, some of these receptors are still unknown. Here we identify the yeast H/KDEL receptor Erd2p as membrane receptor of K28, a viral A/B toxin carrying an HDEL motif at its cell binding β-subunit. While initial toxin binding to the yeast cell wall is unaffected in cells lacking Erd2p, binding to spheroplasts and in vivo toxicity strongly depend on the presence of Erd2p. Consistently, Erd2p is not restricted to membranes of the early secretory pathway but extends to the plasma membrane where it binds and internalizes HDEL-cargo such as K28 toxin, GFP(HDEL) and Kar2p. Since human KDEL receptors are fully functional in yeast and restore toxin sensitivity in the absence of endogenous Erd2p, toxin uptake by H/KDEL receptors at the cell surface might likewise contribute to the intoxication efficiency of A/B toxins carrying a KDEL-motif at their cytotoxic A-subunit(s).
Journal of neurochemistry, Jan 3, 2016
Exocytosis is the process by which stored neurotransmitters and hormones are released via the fus... more Exocytosis is the process by which stored neurotransmitters and hormones are released via the fusion of secretory vesicles with the plasma membrane. It is a dynamic, rapid and spatially restricted process involving multiple steps including vesicle trafficking, tethering, docking, priming and fusion. For many years great steps have been undertaken in our understanding of how exocytosis occurs in different cell types, with significant focus being placed on synaptic release and neurotransmission. However this process of exocytosis is an essential component of cell signalling throughout the body and underpins a diverse array of essential physiological pathways. Many similarities exist between different cell types with regard to key aspects of the exocytosis pathway, such as the need for Ca(2+) to trigger it or the involvement of members of the SNARE protein families. However it is also equally clear that non-neuronal cells have acquired highly specialised mechanisms to control the relea...
F1000 - Post-publication peer review of the biomedical literature, 2007
Duale Reihe Physiologie, 2012
Duale Reihe Physiologie, 2012
Duale Reihe Physiologie, 2012
F1000 - Post-publication peer review of the biomedical literature, 2000
F1000 - Post-publication peer review of the biomedical literature, 2000
The Journal of Immunology, Apr 1, 2009
The Journal of Immunology, Apr 1, 2011
The Journal of Immunology, Apr 1, 2011
The Journal of Immunology, May 1, 2012
Oikos, 1997
Page 1. OIKOS 80: 123-127. Copenhagen 1997 Fluctuating asymmetry indicates levels of competition ... more Page 1. OIKOS 80: 123-127. Copenhagen 1997 Fluctuating asymmetry indicates levels of competition in an even-aged poplar clone Jessica E. Rettig, Rebecca C. Fuller, Andrea L. Corbett and Thomas Getty Rettig, JE, Fuller, RC, Corbett, AL and Getty, T. 1997. ...
Proceedings of the National Academy of Sciences of the United States of America, 2009
The Journal of neuroscience : the official journal of the Society for Neuroscience, Jan 24, 2016
Mutations in the Tulp1 gene cause severe, early-onset retinitis pigmentosa (RP14) in humans. In t... more Mutations in the Tulp1 gene cause severe, early-onset retinitis pigmentosa (RP14) in humans. In the retina, Tulp1 is mainly expressed in photoreceptors that use ribbon synapses to communicate with the inner retina. In the present study, we demonstrate that Tulp1 is highly enriched in the periactive zone of photoreceptor presynaptic terminals where Tulp1 colocalizes with major endocytic proteins close to the synaptic ribbon. Analyses of Tulp1 knock-out mice demonstrate that Tulp1 is essential to keep endocytic proteins enriched at the periactive zone and to maintain high levels of endocytic activity close to the synaptic ribbon. Moreover, we have discovered a novel interaction between Tulp1 and the synaptic ribbon protein RIBEYE, which is important to maintain synaptic ribbon integrity. The current findings suggest a new model for Tulp1-mediated localization of the endocytic machinery at the periactive zone of ribbon synapses and offer a new rationale and mechanism for vision loss as...
The Journal of Immunology, Apr 1, 2009
The Journal of Immunology, May 1, 2012
Scientific Reports, 2016
A/B toxins such as cholera toxin, Pseudomonas exotoxin and killer toxin K28 contain a KDEL-like a... more A/B toxins such as cholera toxin, Pseudomonas exotoxin and killer toxin K28 contain a KDEL-like amino acid motif at one of their subunits which ensures retrograde toxin transport through the secretory pathway of a target cell. As key step in host cell invasion, each toxin binds to distinct plasma membrane receptors that are utilized for cell entry. Despite intensive efforts, some of these receptors are still unknown. Here we identify the yeast H/KDEL receptor Erd2p as membrane receptor of K28, a viral A/B toxin carrying an HDEL motif at its cell binding β-subunit. While initial toxin binding to the yeast cell wall is unaffected in cells lacking Erd2p, binding to spheroplasts and in vivo toxicity strongly depend on the presence of Erd2p. Consistently, Erd2p is not restricted to membranes of the early secretory pathway but extends to the plasma membrane where it binds and internalizes HDEL-cargo such as K28 toxin, GFP(HDEL) and Kar2p. Since human KDEL receptors are fully functional in yeast and restore toxin sensitivity in the absence of endogenous Erd2p, toxin uptake by H/KDEL receptors at the cell surface might likewise contribute to the intoxication efficiency of A/B toxins carrying a KDEL-motif at their cytotoxic A-subunit(s).
Journal of neurochemistry, Jan 3, 2016
Exocytosis is the process by which stored neurotransmitters and hormones are released via the fus... more Exocytosis is the process by which stored neurotransmitters and hormones are released via the fusion of secretory vesicles with the plasma membrane. It is a dynamic, rapid and spatially restricted process involving multiple steps including vesicle trafficking, tethering, docking, priming and fusion. For many years great steps have been undertaken in our understanding of how exocytosis occurs in different cell types, with significant focus being placed on synaptic release and neurotransmission. However this process of exocytosis is an essential component of cell signalling throughout the body and underpins a diverse array of essential physiological pathways. Many similarities exist between different cell types with regard to key aspects of the exocytosis pathway, such as the need for Ca(2+) to trigger it or the involvement of members of the SNARE protein families. However it is also equally clear that non-neuronal cells have acquired highly specialised mechanisms to control the relea...
F1000 - Post-publication peer review of the biomedical literature, 2007
Duale Reihe Physiologie, 2012
Duale Reihe Physiologie, 2012
Duale Reihe Physiologie, 2012
F1000 - Post-publication peer review of the biomedical literature, 2000
F1000 - Post-publication peer review of the biomedical literature, 2000
The Journal of Immunology, Apr 1, 2009
The Journal of Immunology, Apr 1, 2011
The Journal of Immunology, Apr 1, 2011
The Journal of Immunology, May 1, 2012
Oikos, 1997
Page 1. OIKOS 80: 123-127. Copenhagen 1997 Fluctuating asymmetry indicates levels of competition ... more Page 1. OIKOS 80: 123-127. Copenhagen 1997 Fluctuating asymmetry indicates levels of competition in an even-aged poplar clone Jessica E. Rettig, Rebecca C. Fuller, Andrea L. Corbett and Thomas Getty Rettig, JE, Fuller, RC, Corbett, AL and Getty, T. 1997. ...
Proceedings of the National Academy of Sciences of the United States of America, 2009
The Journal of neuroscience : the official journal of the Society for Neuroscience, Jan 24, 2016
Mutations in the Tulp1 gene cause severe, early-onset retinitis pigmentosa (RP14) in humans. In t... more Mutations in the Tulp1 gene cause severe, early-onset retinitis pigmentosa (RP14) in humans. In the retina, Tulp1 is mainly expressed in photoreceptors that use ribbon synapses to communicate with the inner retina. In the present study, we demonstrate that Tulp1 is highly enriched in the periactive zone of photoreceptor presynaptic terminals where Tulp1 colocalizes with major endocytic proteins close to the synaptic ribbon. Analyses of Tulp1 knock-out mice demonstrate that Tulp1 is essential to keep endocytic proteins enriched at the periactive zone and to maintain high levels of endocytic activity close to the synaptic ribbon. Moreover, we have discovered a novel interaction between Tulp1 and the synaptic ribbon protein RIBEYE, which is important to maintain synaptic ribbon integrity. The current findings suggest a new model for Tulp1-mediated localization of the endocytic machinery at the periactive zone of ribbon synapses and offer a new rationale and mechanism for vision loss as...
The Journal of Immunology, Apr 1, 2009
The Journal of Immunology, May 1, 2012