Halécio Designer | ANHANGUERA - Academia.edu (original) (raw)

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Papers by Halécio Designer

Research paper thumbnail of Relationships Between the Abundance of Helminth-Parasites in a Predator, the Sea Lion Otaria-Flavescens Shaw, 1800, and in Its Preys

Research paper thumbnail of Microtubule cytoskeleton behavior in the initial steps of host cell invasion by Besnoitia besnoiti

FEBS Letters, 2006

Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofl... more Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofluorescence showed that isolated B. besnoiti possesses a set of subpellicular microtubules, radiating from the apical end and extending for more than 2/3 of the cell body. Upon interaction with the host cell, B. besnoiti undergoes dramatic modifications of shape and surface, as revealed by atomic force microscopy, accompanied by a distinct tubulin labeling on the posterior region. In the host cell, the microtubule cytoskeleton shows a re-arrangement around the invading parasite suggesting a filamentous interaction with the parasite cytoskeleton during invasion.

Research paper thumbnail of ZoonoticOnchocerca lupiInfection in Dogs, Greece and Portugal, 2011–2012

Emerging Infectious Diseases, 2013

Onchocerca lupi infection is reported primarily in symptomatic dogs. We aimed to determine the in... more Onchocerca lupi infection is reported primarily in symptomatic dogs. We aimed to determine the infection in dogs from areas of Greece and Portugal with reported cases. Of 107 dogs, 9 (8%) were skin snip-positive for the parasite. DNA sequences of parasites in specimens from distinct dog populations differed genetically from those in GenBank. Z oonotic onchocercosis has been attributed to species that primarily infest cattle (Onchocerca gutturosa), horses (O. cervicalis), the European deer (O. jakutensis), and wild boars (O. dewittei japonica) (1). In their definitive hosts, all these species localize in subcutaneous tissues, muscular fasciae, or cervical ligaments, whereas in humans, O. gutturosa and O. cervicalis also have an ocular localization (reviewed in 2). O. lupi is a recently recognized parasite causing nodular lesions associated with ocular disease (i.e., conjunctivitis, ocular swelling, photophobia, lacrimation, discharge, exophthalmia) in dogs (3). The zoonotic potential of this filarioid has been suspected (4) but has only recently been demonstrated in a patient from Turkey (5). Ocular cases in humans are increasingly being reported worldwide, including in Iran (6), Turkey, and Tunisia (7). In addition, O. lupi infection was recently diagnosed near the spinal canal in a 22-monthold child from Arizona, USA (2). Since its first description in a Caucasian wolf (Canis lupus) from Georgia in 1967 (8), O. lupi remained almost

Research paper thumbnail of Identificação morfométrica de larvas infectantes em cultura pura de estrongilídeos de equídeos (Nematoda: Strongylidae)

Morphometric characters for the identification of infective larvae (L3) from Cyathostominae and S... more Morphometric characters for the identification of infective larvae (L3) from Cyathostominae and Strongylinae subfamilies, parasites of equine, are essential for the study of biology, pathology and antihelminthics resistance of nematode parasites responsible for infections ...

Research paper thumbnail of Quantitative Characterization of Solid Epoxy Resins Using Comprehensive Two Dimensional Liquid Chromatography Coupled with Electrospray Ionization-Time of Flight Mass Spectrometry

Analytical Chemistry, 2009

A comprehensive multidimensional liquid chromatography system coupled to Electrospray Ionization-... more A comprehensive multidimensional liquid chromatography system coupled to Electrospray Ionization-Mass Spectrometry (LCxLC-ESI-MS) was developed for detailed characterization and quantitation of solid epoxy resin components. The two orthogonal modes of separation selected were size exclusion chromatography (SEC) in the first dimension and liquid chromatography at critical conditions (LCCC) in the second dimension. Different components present in the solid epoxy resins were separated and quantitated for the first time based on the functional groups and molecular weight heterogeneity. Coupling LCxLC separations with mass spectrometry enabled the identification of components resolved in the two-dimensional space. Several different functional group families of compounds were separated and identified, including epoxy-epoxy and epoxy-r-glycol functional oligomers, and their individual molecular weight ranges were determined. Repeatability obtained ranged from 0.5% for the main product to 21% for oligomers at the 0.4% concentration level. Detailed characterization of synthetic polymers is critical for better understanding of structure-performance relationships. Performance properties are dependent on a number of variables, such as molecular weight distribution, molecular topology, functionality, and chemical composition. A synthetic polymer of specific interest is epoxy resin. To anticipate the types of molecular structures that can be expected in Solid Epoxy Resins (SER) it is necessary to understand some aspects of the manufacturing processes used to make liquid and solid epoxy resins. The following gives a basic description of these processes and suggests some of the molecules and functional groups that can be expected.

Research paper thumbnail of Relationships Between the Abundance of Helminth-Parasites in a Predator, the Sea Lion Otaria-Flavescens Shaw, 1800, and in Its Preys

Research paper thumbnail of Microtubule cytoskeleton behavior in the initial steps of host cell invasion by Besnoitia besnoiti

FEBS Letters, 2006

Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofl... more Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofluorescence showed that isolated B. besnoiti possesses a set of subpellicular microtubules, radiating from the apical end and extending for more than 2/3 of the cell body. Upon interaction with the host cell, B. besnoiti undergoes dramatic modifications of shape and surface, as revealed by atomic force microscopy, accompanied by a distinct tubulin labeling on the posterior region. In the host cell, the microtubule cytoskeleton shows a re-arrangement around the invading parasite suggesting a filamentous interaction with the parasite cytoskeleton during invasion.

Research paper thumbnail of ZoonoticOnchocerca lupiInfection in Dogs, Greece and Portugal, 2011–2012

Emerging Infectious Diseases, 2013

Onchocerca lupi infection is reported primarily in symptomatic dogs. We aimed to determine the in... more Onchocerca lupi infection is reported primarily in symptomatic dogs. We aimed to determine the infection in dogs from areas of Greece and Portugal with reported cases. Of 107 dogs, 9 (8%) were skin snip-positive for the parasite. DNA sequences of parasites in specimens from distinct dog populations differed genetically from those in GenBank. Z oonotic onchocercosis has been attributed to species that primarily infest cattle (Onchocerca gutturosa), horses (O. cervicalis), the European deer (O. jakutensis), and wild boars (O. dewittei japonica) (1). In their definitive hosts, all these species localize in subcutaneous tissues, muscular fasciae, or cervical ligaments, whereas in humans, O. gutturosa and O. cervicalis also have an ocular localization (reviewed in 2). O. lupi is a recently recognized parasite causing nodular lesions associated with ocular disease (i.e., conjunctivitis, ocular swelling, photophobia, lacrimation, discharge, exophthalmia) in dogs (3). The zoonotic potential of this filarioid has been suspected (4) but has only recently been demonstrated in a patient from Turkey (5). Ocular cases in humans are increasingly being reported worldwide, including in Iran (6), Turkey, and Tunisia (7). In addition, O. lupi infection was recently diagnosed near the spinal canal in a 22-monthold child from Arizona, USA (2). Since its first description in a Caucasian wolf (Canis lupus) from Georgia in 1967 (8), O. lupi remained almost

Research paper thumbnail of Identificação morfométrica de larvas infectantes em cultura pura de estrongilídeos de equídeos (Nematoda: Strongylidae)

Morphometric characters for the identification of infective larvae (L3) from Cyathostominae and S... more Morphometric characters for the identification of infective larvae (L3) from Cyathostominae and Strongylinae subfamilies, parasites of equine, are essential for the study of biology, pathology and antihelminthics resistance of nematode parasites responsible for infections ...

Research paper thumbnail of Quantitative Characterization of Solid Epoxy Resins Using Comprehensive Two Dimensional Liquid Chromatography Coupled with Electrospray Ionization-Time of Flight Mass Spectrometry

Analytical Chemistry, 2009

A comprehensive multidimensional liquid chromatography system coupled to Electrospray Ionization-... more A comprehensive multidimensional liquid chromatography system coupled to Electrospray Ionization-Mass Spectrometry (LCxLC-ESI-MS) was developed for detailed characterization and quantitation of solid epoxy resin components. The two orthogonal modes of separation selected were size exclusion chromatography (SEC) in the first dimension and liquid chromatography at critical conditions (LCCC) in the second dimension. Different components present in the solid epoxy resins were separated and quantitated for the first time based on the functional groups and molecular weight heterogeneity. Coupling LCxLC separations with mass spectrometry enabled the identification of components resolved in the two-dimensional space. Several different functional group families of compounds were separated and identified, including epoxy-epoxy and epoxy-r-glycol functional oligomers, and their individual molecular weight ranges were determined. Repeatability obtained ranged from 0.5% for the main product to 21% for oligomers at the 0.4% concentration level. Detailed characterization of synthetic polymers is critical for better understanding of structure-performance relationships. Performance properties are dependent on a number of variables, such as molecular weight distribution, molecular topology, functionality, and chemical composition. A synthetic polymer of specific interest is epoxy resin. To anticipate the types of molecular structures that can be expected in Solid Epoxy Resins (SER) it is necessary to understand some aspects of the manufacturing processes used to make liquid and solid epoxy resins. The following gives a basic description of these processes and suggests some of the molecules and functional groups that can be expected.