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Papers by Lorenzo Polimeno

Cancer Research, Nov 1, 1987

Proceedings of the National Academy of Sciences of the United States of America, Aug 16, 1994

Digestive and Liver Disease, Apr 1, 2006

Endocrine, metabolic & immune disorders, Jun 1, 2022

Background: Bisphenol A (BPA), an important industrial material widely applied in daily products,... more Background: Bisphenol A (BPA), an important industrial material widely applied in daily products, is considered an endocrine-disrupting chemical that may adversely affect humans. Grow-ing evidence has shown that intestinal bacterial alterations caused by BPA exposure play an im-portant role in several local and systemic diseases. Aim: finding evidence that BPA-induced alterations in gut microbiota composition and activity may perturb its role on human health. Results: Evidence from several experimental settings shows that both low and high doses of BPA in-terfere with the hormonal, homeostatic, and reproductive systems in animals and humans. Moreover, it has recently been classified as an environmental obesogenic, with metabolic-disrupting effects on lipid metabolism and pancreatic b-cell functions. Several evidence characterizes PBA as an envi-ronmental contributor to type II diabetes, metabolic syndromes, and obesity. However, the highest estimates of the exposure derived from foods alone or in combination with other sources are 3 to 5 times below the new tolerable daily intake (TDI) value, today reduced by the European Food Safety Authority (EFSA) experts from 50 micrograms per kilogramme of bodyweight per day (μg/kg bw/day) to 4 μg/kg bw/day. Conclusions: Considering estimates for the total amount of BPA that can be ingested daily over a lifetime, many International Health Authorities conclude that dietary exposure of adult humans to BPA does not represent a risk to consumers' health, declaring its safety due to very-low established levels in food and water and any appreciable health risk.

Digestive and Liver Disease, Mar 1, 2009

Endocrine, metabolic & immune disorders, May 1, 2023

: Oxidative stress is a major cellular and metabolic burden that can really alter cell life and b... more : Oxidative stress is a major cellular and metabolic burden that can really alter cell life and become the base for disease onset and development. Many widespread pathologies can develop from an unresolved oxidative stress situation; thus, addressing this state is paramount for human health. Our antioxidant enzymes sometimes are not just enough. Fortifying our defense and the antioxidant and anti-inflammatory system can make a difference in our health: if this is attainable with our dietary habits, it could be a dream come true. Polyphenols are a fantastic tool indeed in the fight against oxidative stress: they are easy to obtain, with little cost, no side effects, and have a multitude of metabolic actions. This perspective review would shed light on polyphenol’s metabolic and molecular action regarding oxidative stress to help preserve our health.

The Italian journal of gastroenterology, 1978

Two individuals undergoing orthotopic hepatic transplantation received livers from donors who wer... more Two individuals undergoing orthotopic hepatic transplantation received livers from donors who were on average 10 kg smaller than themselves based on recipient ideal body weight. As a result, the donor livers in these 2 cases were 29%~59% smaller than would be expected had the donor liver and recipient been matched ideally. The liver grafts in the recipients steadily increased in size, as determined by serial computed tomography scanning, to achieve new volumes consistent with those that would have been expected in a normal individual of the recipient’s size, sex, and age. Fasting plasma levels of amino acids, glucagon, insulin, and standard liver injury tests were monitored to determine which measure best reflected the changes observed in the size of the grafts over time. No relationship between the changes observed in any of these parameters and hepatic growth was apparent. In both cases, the liver increased in volume at a rate of -70 ml/day. These data demonstrate that a small-for-size liver transplanted into a larger recipient increases in size at a rate of -70 ml/day until it achieves a liver volume consistent with that expected given the recipient’s size, age, and sex. Under normal circumstances, attempts at matching the recipient of an orthotopic liver transplant (OLT) with the best available donor in terms of organ and body size are made (12). Occasionally, however, only a small donor is available and thus a small-for

Digestive and Liver Disease, Nov 1, 2000

European journal of medicinal chemistry, Jan 19, 2016

An affinity capillary electrophoresis (ACE) method to estimate apparent dissociation constants be... more An affinity capillary electrophoresis (ACE) method to estimate apparent dissociation constants between bovine brain calmodulin (CaM) and non-peptidic ligands was developed. The method was validated reproducing the dissociation constants of a number of well-known CaM ligands. In particular, the potent antagonist 125-C9 was ad hoc synthesized through an improved synthetic procedure. The ACE method was successfully applied to verify CaM affinity for lubeluzole, a well-known neuroprotective agent recently proved useful to potentiate the activity of anti-cancer drugs. Lubeluzole was slightly less potent than 125-C9 (Kd = 2.9 ± 0.7 and 0.47 ± 0.06 μM, respectively) and displayed Ca(2+)/calmodulin-dependent kinase II (CaMKII) inhibition (IC50 = 40 ± 1 μM). Possible binding modes of lubeluzole to CaM were explored by docking studies based on the X-ray crystal structures of several trifluoperazine-CaM complexes. An estimated dissociation constant in good agreement with the experimental one w...

The Italian journal of gastroenterology

Cancer Research, Nov 1, 1987

Proceedings of the National Academy of Sciences of the United States of America, Aug 16, 1994

Digestive and Liver Disease, Apr 1, 2006

Endocrine, metabolic & immune disorders, Jun 1, 2022

Background: Bisphenol A (BPA), an important industrial material widely applied in daily products,... more Background: Bisphenol A (BPA), an important industrial material widely applied in daily products, is considered an endocrine-disrupting chemical that may adversely affect humans. Grow-ing evidence has shown that intestinal bacterial alterations caused by BPA exposure play an im-portant role in several local and systemic diseases. Aim: finding evidence that BPA-induced alterations in gut microbiota composition and activity may perturb its role on human health. Results: Evidence from several experimental settings shows that both low and high doses of BPA in-terfere with the hormonal, homeostatic, and reproductive systems in animals and humans. Moreover, it has recently been classified as an environmental obesogenic, with metabolic-disrupting effects on lipid metabolism and pancreatic b-cell functions. Several evidence characterizes PBA as an envi-ronmental contributor to type II diabetes, metabolic syndromes, and obesity. However, the highest estimates of the exposure derived from foods alone or in combination with other sources are 3 to 5 times below the new tolerable daily intake (TDI) value, today reduced by the European Food Safety Authority (EFSA) experts from 50 micrograms per kilogramme of bodyweight per day (μg/kg bw/day) to 4 μg/kg bw/day. Conclusions: Considering estimates for the total amount of BPA that can be ingested daily over a lifetime, many International Health Authorities conclude that dietary exposure of adult humans to BPA does not represent a risk to consumers' health, declaring its safety due to very-low established levels in food and water and any appreciable health risk.

Digestive and Liver Disease, Mar 1, 2009

Endocrine, metabolic & immune disorders, May 1, 2023

: Oxidative stress is a major cellular and metabolic burden that can really alter cell life and b... more : Oxidative stress is a major cellular and metabolic burden that can really alter cell life and become the base for disease onset and development. Many widespread pathologies can develop from an unresolved oxidative stress situation; thus, addressing this state is paramount for human health. Our antioxidant enzymes sometimes are not just enough. Fortifying our defense and the antioxidant and anti-inflammatory system can make a difference in our health: if this is attainable with our dietary habits, it could be a dream come true. Polyphenols are a fantastic tool indeed in the fight against oxidative stress: they are easy to obtain, with little cost, no side effects, and have a multitude of metabolic actions. This perspective review would shed light on polyphenol’s metabolic and molecular action regarding oxidative stress to help preserve our health.

The Italian journal of gastroenterology, 1978

Two individuals undergoing orthotopic hepatic transplantation received livers from donors who wer... more Two individuals undergoing orthotopic hepatic transplantation received livers from donors who were on average 10 kg smaller than themselves based on recipient ideal body weight. As a result, the donor livers in these 2 cases were 29%~59% smaller than would be expected had the donor liver and recipient been matched ideally. The liver grafts in the recipients steadily increased in size, as determined by serial computed tomography scanning, to achieve new volumes consistent with those that would have been expected in a normal individual of the recipient’s size, sex, and age. Fasting plasma levels of amino acids, glucagon, insulin, and standard liver injury tests were monitored to determine which measure best reflected the changes observed in the size of the grafts over time. No relationship between the changes observed in any of these parameters and hepatic growth was apparent. In both cases, the liver increased in volume at a rate of -70 ml/day. These data demonstrate that a small-for-size liver transplanted into a larger recipient increases in size at a rate of -70 ml/day until it achieves a liver volume consistent with that expected given the recipient’s size, age, and sex. Under normal circumstances, attempts at matching the recipient of an orthotopic liver transplant (OLT) with the best available donor in terms of organ and body size are made (12). Occasionally, however, only a small donor is available and thus a small-for

Digestive and Liver Disease, Nov 1, 2000

European journal of medicinal chemistry, Jan 19, 2016

An affinity capillary electrophoresis (ACE) method to estimate apparent dissociation constants be... more An affinity capillary electrophoresis (ACE) method to estimate apparent dissociation constants between bovine brain calmodulin (CaM) and non-peptidic ligands was developed. The method was validated reproducing the dissociation constants of a number of well-known CaM ligands. In particular, the potent antagonist 125-C9 was ad hoc synthesized through an improved synthetic procedure. The ACE method was successfully applied to verify CaM affinity for lubeluzole, a well-known neuroprotective agent recently proved useful to potentiate the activity of anti-cancer drugs. Lubeluzole was slightly less potent than 125-C9 (Kd = 2.9 ± 0.7 and 0.47 ± 0.06 μM, respectively) and displayed Ca(2+)/calmodulin-dependent kinase II (CaMKII) inhibition (IC50 = 40 ± 1 μM). Possible binding modes of lubeluzole to CaM were explored by docking studies based on the X-ray crystal structures of several trifluoperazine-CaM complexes. An estimated dissociation constant in good agreement with the experimental one w...

The Italian journal of gastroenterology