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Papers by Ojonoma Loretta Okwute

Academic Journals, Dec 31, 2007

Various concentrations (1, 5 and 10 %) of milled oven-dried ginger and fresh ginger were incorpor... more Various concentrations (1, 5 and 10 %) of milled oven-dried ginger and fresh ginger were incorporated into ogi at the beginning of fermentation. Physicochemical and microbiological changes were noted during fermentation. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of ethanolic ginger extract on selected bacterial isolates were also investigated. Physicochemical parameters such as pH and Titratable acid were analysed while microbiological changes were observed by inoculation of the various concentrations on Nutrient Agar, Salmonella-Shigella agar using the spread plate method. The pH of ogi samples decreased steadily and ranged between 4.11 at the beginning of fermentation and 5.74 at the end of fermentation and Titratable acidity (TA) ranged between 0.22 at the beginning of fermentation (0 hr) and 0.75 % at the end of fermentation (72 hrs). The total viable and coliform counts ranged between 2.8 and 7.3 (x10 5 cfu/ml) and 2.8 and 5.9 (...

The effects of palm oil mill effluent (POME) on the total aerobic bacterial populations and ammon... more The effects of palm oil mill effluent (POME) on the total aerobic bacterial populations and ammonium oxidizers in the soil were assessed. This was done by culturing soil samples from an effluent dumpsite for the total aerobic bacterial counts and ammonium oxidizers. Results showed that the total aerobic bacterial populations in the POME soil samples (9.6 x 108 ± 0.3 at 20oC, 1.64 x 109 ± 0.2 at 30oC and 1.07 x 109 ± 0.4 at 40oC) were significantly higher (P 0.05) than the counts for the non-POME soil samples (4.5 x 108 ± 0.3 at 20oC, 7.6 x 108 ± 0.3 at 30oC and 5.9 x 108 ± 0.3 at 40oC). In addition, ammonium oxidizers were isolated from the non-POME soil samples but not from the POME soil samples.

The aim of this study was to demonstrate the use of chicken droppings and cow dung in the amendme... more The aim of this study was to demonstrate the use of chicken droppings and cow dung in the amendment of soil polluted with palm oil mill effluent (POME) in bioremediation. Soil polluted with 20 % raw (POME) in the laboratory was amended with different concentrations of chicken droppings, cow dung and a combination of the wastes (10 %, 20 % and 30 %). Isolation, characterization and identification of microorganisms were carried out and compared over time with respect to the different concentrations. Gas chromatography mass spectroscopy (GCMS) analysis of extracts of POME polluted and amended soil indicated a reduction in the number of long chain hydrocarbons (C13-C44) in POME polluted soil to C8-C21 in amended soil. This was attributed to the presence of microorganisms of the genera Pseudomonas, Bacillus, Proteus, Micrococcus, Aspergillus, Penicillium, Paecilomyces and Candida in significant numbers throughout the period of analysis. However, a combination of the two organic wastes at...

African Journal of Biotechnology, 2008

British Biotechnology Journal, 2014

The aim of this study was to demonstrate the use of chicken droppings and cow dung in the amendme... more The aim of this study was to demonstrate the use of chicken droppings and cow dung in the amendment of soil polluted with palm oil mill effluent (POME) in bioremediation. Soil polluted with 20 % raw (POME) in the laboratory was amended with different concentrations of chicken droppings, cow dung and a combination of the wastes (10 %, 20 % and 30 %). Isolation, characterization and identification of microorganisms were carried out and compared over time with respect to the different concentrations. Gas chromatography mass spectroscopy (GCMS) analysis of extracts of POME polluted and amended soil indicated a reduction in the number of long chain hydrocarbons (C 13 -C 44 ) in POME polluted soil to C 8 -C 21 in amended soil. This was attributed to the presence of microorganisms of the genera Pseudomonas, Bacillus, Proteus, Micrococcus, Aspergillus, Penicillium, Paecilomyces and Candida in significant numbers throughout the period of analysis. However, a combination of the two organic wastes at 20 % concentration was most effective in this reduction. The implication of these findings is that the bacteria, mould and yeast isolates found in these organic wastes can be useful in rehabilitation of POME polluted soil and possibly other oil polluted sites.

To assess changes in microbial population in palm oil mill effluent (POME) polluted soil amended ... more To assess changes in microbial population in palm oil mill effluent (POME) polluted soil amended with chicken droppings and cow dung. Study Design: 32 plots measuring 4 m 2 were mapped out in a randomized complete block design of five main plots with three replicates. Data collected were subjected to ANOVA using SPSS. Place and Duration of Study:

Giardiasis is an acute form of gastroenteritis caused by the parasite Giardia lamblia. An increas... more Giardiasis is an acute form of gastroenteritis caused by the parasite Giardia lamblia. An increase in the incidence and frequency of the disease in the last few years in the developed world has brought to the fore a now recognized mode of transmission -sexual contact. This in turn has led to giardiasis being classified as a sexually transmitted disease by the Centre for Disease Control and Prevention (CDC) in the United States. This review identifies its occurrence mainly in homosexual populations of the developed world especially in the United States and the possible spread of the disease through the family setting and even heterosexual relationships.

The effect of POME on the integrity of the soil was investigated. Soil samples from the palm oil ... more The effect of POME on the integrity of the soil was investigated. Soil samples from the palm oil mill effluent (POME) dumpsite as well as a non-POME site were tested for physico-chemical properties such as pH, water holding capacity, available phosphorus, organic carbon, total nitrogen, mineral assay and cation exchange capacity. Furthermore, the total aerobic bacteria counts of the samples at 2, 30 and 40 o C were assayed. Results showed significant differences (P < 0.05) and (P < 0.01) in pH, water holding capacity, organic carbon, total nitrogen, cation exchange capacity and available phosphorus. 30 o C had the highest average microbial bioload (1.64 x 10 9 ± 0.2) and so, the most favourable for growth. Bacterial counts from the POME dumpsite were found to be significantly higher (P < 0.05),

Academic Journals, Dec 31, 2007

African Journal of Biotechnology, 2008

British Biotechnology Journal, 2014

The aim of this study was to demonstrate the use of chicken droppings and cow dung in the amendme... more The aim of this study was to demonstrate the use of chicken droppings and cow dung in the amendment of soil polluted with palm oil mill effluent (POME) in bioremediation. Soil polluted with 20 % raw (POME) in the laboratory was amended with different concentrations of chicken droppings, cow dung and a combination of the wastes (10 %, 20 % and 30 %). Isolation, characterization and identification of microorganisms were carried out and compared over time with respect to the different concentrations. Gas chromatography mass spectroscopy (GCMS) analysis of extracts of POME polluted and amended soil indicated a reduction in the number of long chain hydrocarbons (C 13 -C 44 ) in POME polluted soil to C 8 -C 21 in amended soil. This was attributed to the presence of microorganisms of the genera Pseudomonas, Bacillus, Proteus, Micrococcus, Aspergillus, Penicillium, Paecilomyces and Candida in significant numbers throughout the period of analysis. However, a combination of the two organic wastes at 20 % concentration was most effective in this reduction. The implication of these findings is that the bacteria, mould and yeast isolates found in these organic wastes can be useful in rehabilitation of POME polluted soil and possibly other oil polluted sites.