Regina PUPPIN-RONTANI | Universidade Estadual de Campinas (original) (raw)

Papers by Regina PUPPIN-RONTANI

Research paper thumbnail of Polishing techniques effect on microhybrid, nanohybrid and nanofilled composites color and surface roughness stability

Research paper thumbnail of Obesity and dental caries--A systematic review

Oral Health Preventive Dentistry, Feb 1, 2006

Research paper thumbnail of Marginal adaptation of pit and fissure sealants after thermal and chemical stress. A SEM study

American Journal of Dentistry, Dec 1, 2008

To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer m... more To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer materials (resin sealant, glass-ionomer cement, resin-modified glass-ionomer cement, and adhesive system) submitted to thermal and chemical stress, using scanning electron microscopy evaluation (SEM). 80 impacted human third molars were randomly assigned to the following experimental groups (n = 10): FluroShield (F), Helioseal Clear Chroma (H), Vitremer (V), Fuji II-LC (FII), Ketac-Molar (KM), Fuji IX (FIX), Single Bond (SB), and Clearfil Protect Bond (CF). All groups were subjected to thermocycling and 14 days of pH cycling. A blinded and calibrated examiner performed SEM analysis. Gap formation was scored according to: 0 = no sealant marginal gaps; 1 = sealant marginal gaps present or total sealant loss. The score 0 was considered a success, while score 1 represented failure. Data were analyzed by Kruskal-Wallis and Bonferroni tests (P < 0.05). The success rates of SB (100%) and V (90%) were similar, but statistically superior to F (30%), H (20%), FIX (20%), and CF (0%) (P < 0.05). A tendency for similar behaviors of FII and KM to SB and V was observed and for similar behaviors of F, FIX and H to CF. The worst results were obtained for CF.

Research paper thumbnail of Bonding degradation by thermo-mechanical cycling on chlorhexidine and MDPB presence

Research paper thumbnail of Minimally invasive dentistry: bond strength of different sealant and filling materials to enamel

Oral health & preventive dentistry

The purpose of this study was to evaluate the shear bond strength of different sealant and fillin... more The purpose of this study was to evaluate the shear bond strength of different sealant and filling materials, used in minimally invasive dentistry, to human enamel. Thirty-five sound extracted third molars were selected. The crowns were longitudinally sectioned, embedded in polystyrene resin, and grounded until a flat enamel surface was reached. The samples were assigned into seven groups (n = 10), according to the materials: G1-Fluoroshield; G2-Clinpro; G3-Dyract AP; G4-F2000; G5-Vitremer; G6-Fuji IX; G7-Vidrion F. All materials were applied according to the manufacturer's instructions. The samples were stored in distilled water at 37 degrees C for 24 h and submitted to a shear bonding strength test in a universal testing machine with a crosshead speed of 0.5 mm/min. The failure sites were observed in Scanning Electron Microscopy (SEM). The data were submitted to ANOVA and Tukey's tests (p < 0.05). The mean values (MPa) of shear bond strength were for Fluroshield (25.92 ...

Research paper thumbnail of Photoinitiator chemistry and concentratrion: Effect on polymerization-characteristics and mechanical-properties

Objective: The aim of this study was to determine the influence of photoinitiator chemistry and c... more Objective: The aim of this study was to determine the influence of photoinitiator chemistry and concentration on polymerization characteristics (degree of conversion, crosslink density) and mechanical properties (hardness, elastic modulus) of experimental composites photocured by different methods. Methods: Composites based on BisGMA/TEGDMA and 65% of feldspar filler were prepared with different type and concentration of photoinitiators (camphorquinone (CQ), phenylpropanedione (PPD), bisacylphosphine oxide (BAPO) or CQ/PPD, CQ/BAPO at concentrations of 1% or 2%). Bar specimens were prepared (n=10) and all tests were conducted at the same specimens. Degree of conversion (DC) was determined by Fourier Transform Infrared Spectroscopy (FTIR). Knoop Hardness (KHN)was assessed using un indenter Shimadzu HMV with load of 50g for 5s. Elastic modulus (E) was evaluated through a three point bending flexural test in a Universal testing machine. An indirect evaluation of crosslink density (CLD)...

Research paper thumbnail of Atuação interdisciplinar odontologia/fonoaudiologia no tratamento de paciente com cárie precoce da infância

Revista CEFAC, 2015

ABSTRACT

Research paper thumbnail of Microtensile bond strength of indirect composite restorations using different combinations of resin coating technique

Brazilian Dental Science, 2012

Research paper thumbnail of Atraumatic restorative treatment in Brazilian schoolchildren: 12 months preliminary clinical results

Brazilian Dental Science, 2014

Research paper thumbnail of Comparative Analysis of Root Dentin Morphology and Structure of Human Versus Bovine Primary Teeth

Microscopy and Microanalysis, 2015

This study evaluated the structural and morphological differences between human and bovine primar... more This study evaluated the structural and morphological differences between human and bovine primary root canals. Primary human maxillary central incisors (H) (n=9) and primary bovine incisors (B) (n=9) were selected. The roots were sectioned in the vestibular-lingual direction, planed and delimited in cervical, middle, and apical thirds. Tubule density (number of tubules per mm2) and diameter were analyzed by scanning electron microscopy (1,000 and 5,000×) using Image J 1.47 software. Data were submitted to two-way repeated measures ANOVA and Tukey tests (α=0.05). The highest tubule density was observed for B (28.527±1.717 mm2) compared with H (15.931±0.170 mm2) (p&amp;amp;amp;amp;lt;0.01). Regarding root thirds, the cervical third presented a greater tubule density (26.417±11.654 mm2) than the apical third (17.999±5.873 mm2). The diameter of the dentin tubules was not different for cervical (3.50±0.08 µm), middle (3.45±0.30 µm) and apical thirds (3.42±0.33 µm) and substrate (H-3.29±0.14 µm; B-3.63±0.06 µm). It could be concluded that: (1) the radicular dentin structure of human and bovine primary teeth and root thirds differ in terms of the tubule density; (2) the radicular dentin morphology of human and bovine primary teeth and root thirds are similar in terms of the diameter of the dentin tubules.

Research paper thumbnail of Effect of cleansers and irrigation methods on primary root dentin permeability

Journal of dentistry for children (Chicago, Ill.)

The purpose of this study was to evaluate the effects of cleansers and irrigation methods on the ... more The purpose of this study was to evaluate the effects of cleansers and irrigation methods on the permeability index (PI) in primary root dentin. One hundred twelve teeth were extracted and sectioned transversely, discarding their crowns. Then, the roots were distributed according to the cleansers: (1) Dakin (D); (2) Dakin+hydrogen peroxide (DHP); (3) 2% chlorhexidine gel (C); and (4) saline (S). The canals were manually instrumented. Irrigation was performed manually (MI) or ultrasonically (UI). The roots were: (1) made impermeable; (2) filled with 2% methylene blue solution; and (3) longitudinally sectioned. The halves were marked in thirds (cervical, middle, and apical), and the areas of dye penetration were measured. PI data were submitted to factorial analysis of variance and Tukey tests (P<.05). A significant effect of MI/UI, in association with cleansers, was seen on PI. For all thirds, the PI of MI was superior to UI. In the cervical third, MI achieved a high PI when assoc...

Research paper thumbnail of Effects of water storage on bond strength and dentin sealing ability promoted by adhesive systems

The journal of adhesive dentistry, 2012

To evaluate the dentin bond strength (BS) and sealing ability (SA) promoted by adhesive systems a... more To evaluate the dentin bond strength (BS) and sealing ability (SA) promoted by adhesive systems after 24 h or 6 months of water storage. The tested adhesive systems were: one three-step etch-and-rinse adhesive (Adper Scotchbond Multi-Purpose, SBMP) and three single-step self-etching systems (Adper Easy Bond, Bond Force, and G-Bond Plus). Bovine incisors were used for both evaluations, BS (n = 11) and SA (n = 5). To examine BS, the buccal surface was ground with SiC paper to expose a flat dentin surface. After adhesive application, a block of resin composite was incrementally built up over the bonded surface and sectioned into sticks. These bonded specimens were subjected to microtensile bond strength testing after 24 h and 6 months of water storage using a universal testing machine. For SA analysis, enamel was removed from the buccal surfaces. The teeth were connected to a device to measure the initial SA (10 psi), and the second measurement was taken after treating dentin with EDTA...

Research paper thumbnail of Permeability and smear layer removal: effects of different chemical agents on the primary root dentin

Pediatric dentistry

The purpose of this study was to evaluate the effects of chemical agents on the permeability and ... more The purpose of this study was to evaluate the effects of chemical agents on the permeability and removal of the smear layer in the primary root dentin. Fifty roots were distributed into 6 groups: (1) 5.25% sodium hypochlorite (SH); (2) 5.25% SH+17% ethylenedia-minetetraacetic acid (SHE); (3) 2% chlorhexidine (CHX); (4) 2% CHX+17% ethylenediaminetetraacetic acid (CHXE); (5) 17% EDTA (E); and (6) saline solution (SS). The canals were instrumented and irrigated. The roots were dyed, washed, and sectioned, and the permeability index was measured. The amount of smear layer was scored. Data were submitted to analysis of variance, Kruskal-Wallis, Student-Newman-Keuls and t tests (P<.05). Significant differences were observed in the cervical and apical thirds for the CHX (P=.03), CHXE (P=.027), SS (P=.038), and SH (P<.001) groups. The SS group showed a heavy smear layer in all thirds. Root canals treated with SHE showed great cleaning in the cervical, middle, and apical thirds. Sodium...

Research paper thumbnail of One-year clinical evaluation of oral rehabilitation after the loss of multiple primary teeth

General dentistry

This article describes a one-year follow-up of the prosthetic rehabilitation of a child who lost ... more This article describes a one-year follow-up of the prosthetic rehabilitation of a child who lost multiple primary teeth due to widespread dental caries. Clinical and radiographic examinations indicated the need for all primary teeth to be extracted. Following the extractions, complete maxillary and mandibular removable prostheses with individualized occlusion and age-appropriate artificial teeth were fitted for functional and esthetic rehabilitation. The treatment improved the patient's speech, masticatory function, and facial esthetics, contributing to the development of normal dietary habits and social integration.

Research paper thumbnail of Effects of acidic primer/adhesives on primary and permanent dentin

American journal of dentistry, 2009

To evaluate the quality of primary and permanent dentin by Fourier transformed Raman spectroscopy... more To evaluate the quality of primary and permanent dentin by Fourier transformed Raman spectroscopy (FT-Raman), and scanning electron microscopy/energy-dispersive spectroscopy (SEM/EDS). The middle dentin of crowns was reached by carbide bur abrading providing a uniform smear layer. Self-etching primers were applied in order to simulate the etching of self-etching adhesive systems. The groups were (n = 6): G1 (primary dentin smear layer); G2 (primary dentin etched by primer of Clearfil Protect Bond); G3 (primary dentin etched by Adper Prompt); G4 (permanent dentin smear layer); G5 (permanent dentin etched by primer of Clearfil Protect Bond); G6 (permanent dentin etched by Adper Prompt). SEM/EDS were made in order to obtain additional elemental data to complement FT-Raman. FT-Raman data were submitted to cluster analysis. Overall, FT-Raman showed differences between primary and permanent dentin concerning organic content, but not for inorganic content. FT-Raman showed differences in th...

Research paper thumbnail of Marginal adaptation of pit and fissure sealants after thermal and chemical stress. A SEM study

American journal of dentistry, 2008

To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer m... more To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer materials (resin sealant, glass-ionomer cement, resin-modified glass-ionomer cement, and adhesive system) submitted to thermal and chemical stress, using scanning electron microscopy evaluation (SEM). 80 impacted human third molars were randomly assigned to the following experimental groups (n = 10): FluroShield (F), Helioseal Clear Chroma (H), Vitremer (V), Fuji II-LC (FII), Ketac-Molar (KM), Fuji IX (FIX), Single Bond (SB), and Clearfil Protect Bond (CF). All groups were subjected to thermocycling and 14 days of pH cycling. A blinded and calibrated examiner performed SEM analysis. Gap formation was scored according to: 0 = no sealant marginal gaps; 1 = sealant marginal gaps present or total sealant loss. The score 0 was considered a success, while score 1 represented failure. Data were analyzed by Kruskal-Wallis and Bonferroni tests (P < 0.05). The success rates of SB (100%) and V (9...

Research paper thumbnail of Is the presence of the smear layer a limiting factor for root dentin permeability in primary teeth?

Journal of dentistry for children (Chicago, Ill.)

The purpose of this study was to correlate the dye permeability to the morphological aspect (pres... more The purpose of this study was to correlate the dye permeability to the morphological aspect (presence or absence of a smear layer) of the primary root dentin wall, using scanning electron microscopy, regarding the endodontic preparation and irrigation methods. The hypothesis evaluated was that there was a correlation between the dye permeability and the morphological aspect of the primary root dentin. A total of 112 extracted primary roots were distributed into the following groups: Dakin's liquid, Dakin+hydrogen peroxide; 2% chlorhexidine gel; and saline solution. Manual (MI) or Ultrasonic irrigation (UI) was performed. The roots were made impermeable, filled with dye (2% methylene blue), and longitudinally sectioned. The halves were divided in cervical, middle, and apical thirds for dye penetration measurement. The samples were observed under a scanning electron microscope. The data were submitted to linear regression analysis with a dummy variable (P<.05). The data reveale...

Research paper thumbnail of In vitro wear of primary and permanent enamel. Simultaneous erosion and abrasion

American journal of dentistry, 2007

To evaluate the wear differences between primary and permanent enamel caused by the association o... more To evaluate the wear differences between primary and permanent enamel caused by the association of abrasion and erosion. Fragments of 40 human teeth (20 primary and 20 permanent) were cast in acrylic rings, polished to expose a flat enamel area, and evaluated with a 3D profiler. Antagonists were made from primary and permanent molars. The specimens were distributed into four groups (n = 10) according to type of substrate and slurry (neutral and acidic), and cycled 100,000 times in the OHSU oral wear simulator. Specimens were cleaned and re-profiled. Volume loss and maximum depth were determined on the polished specimens. Data were submitted to ANOVA and Tukey's test. The area of the wear facet on the antagonist was measured. Primary enamel showed more wear than permanent enamel, regardless of the slurry type. The acidic environment increased the wear rate compared to the neutral slurry. The association of abrasion and erosion increased the wear rate for the primary teeth only. T...

Research paper thumbnail of The influence of cleansers on the permeability index of primary tooth root dentin

The Journal of clinical pediatric dentistry, 2006

The aim of this study was to evaluate the action of cleansers on the permeability index (PI) in t... more The aim of this study was to evaluate the action of cleansers on the permeability index (PI) in the root canal of primary dentin, by dye penetration into the length of the root canal and deep dentin. The data were submitted to ANOVA, F, and Student t tests (p<0.05). Lengthwise, chlorhexidine 1% and 2% presented significantly higher mean values than Dakin's liquid. Considering depth, in the cervical third, chlorhexidine 1% and 2% mean values were higher than Dakin's liquid+urea peroxide and Dakin's liquid mean values; distilled water mean values were higher than Dakin's liquid. In the middle and apical thirds, chlorhexidine 1% and 2% mean values were significantly the highest.

Research paper thumbnail of In vitro wear evaluation of dental materials in primary teeth

American journal of dentistry, 2006

To evaluate the effect of dental materials on primary enamel wear. Four composites (Filtek Z250, ... more To evaluate the effect of dental materials on primary enamel wear. Four composites (Filtek Z250, Heliomolar, Point 4, SureFil), two compomers (Dyract AP, F2000) and a resin-modified glass-ionomer (Vitremer) were placed into box-shaped preparations in buccal surfaces of primary molars and stored for 24 hours in water at 37 degrees C. The specimens (n = 6) were subjected to abrasive three-body wear against a primary enamel cusp. Material wear, enamel wear (adjacent to the material), and wear on enamel cusps were estimated by profilometry and video-analysis software. Results were compared with ANOVA and Tukey's tests (P < 0.05). Vitremer showed the highest wear (P < 0.05), followed by Dyract AP and F2000, which were not statistically different (P > 0.05). There was no significant difference between Filtek Z250 and Dyract AP (P > 0.05). Filtek Z250, Heliomolar, Point 4 and SureFil, showed the lowest wear values and there was no difference among them (P > 0.05). The hi...

Research paper thumbnail of Polishing techniques effect on microhybrid, nanohybrid and nanofilled composites color and surface roughness stability

Research paper thumbnail of Obesity and dental caries--A systematic review

Oral Health Preventive Dentistry, Feb 1, 2006

Research paper thumbnail of Marginal adaptation of pit and fissure sealants after thermal and chemical stress. A SEM study

American Journal of Dentistry, Dec 1, 2008

To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer m... more To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer materials (resin sealant, glass-ionomer cement, resin-modified glass-ionomer cement, and adhesive system) submitted to thermal and chemical stress, using scanning electron microscopy evaluation (SEM). 80 impacted human third molars were randomly assigned to the following experimental groups (n = 10): FluroShield (F), Helioseal Clear Chroma (H), Vitremer (V), Fuji II-LC (FII), Ketac-Molar (KM), Fuji IX (FIX), Single Bond (SB), and Clearfil Protect Bond (CF). All groups were subjected to thermocycling and 14 days of pH cycling. A blinded and calibrated examiner performed SEM analysis. Gap formation was scored according to: 0 = no sealant marginal gaps; 1 = sealant marginal gaps present or total sealant loss. The score 0 was considered a success, while score 1 represented failure. Data were analyzed by Kruskal-Wallis and Bonferroni tests (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). The success rates of SB (100%) and V (90%) were similar, but statistically superior to F (30%), H (20%), FIX (20%), and CF (0%) (P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). A tendency for similar behaviors of FII and KM to SB and V was observed and for similar behaviors of F, FIX and H to CF. The worst results were obtained for CF.

Research paper thumbnail of Bonding degradation by thermo-mechanical cycling on chlorhexidine and MDPB presence

Research paper thumbnail of Minimally invasive dentistry: bond strength of different sealant and filling materials to enamel

Oral health & preventive dentistry

The purpose of this study was to evaluate the shear bond strength of different sealant and fillin... more The purpose of this study was to evaluate the shear bond strength of different sealant and filling materials, used in minimally invasive dentistry, to human enamel. Thirty-five sound extracted third molars were selected. The crowns were longitudinally sectioned, embedded in polystyrene resin, and grounded until a flat enamel surface was reached. The samples were assigned into seven groups (n = 10), according to the materials: G1-Fluoroshield; G2-Clinpro; G3-Dyract AP; G4-F2000; G5-Vitremer; G6-Fuji IX; G7-Vidrion F. All materials were applied according to the manufacturer's instructions. The samples were stored in distilled water at 37 degrees C for 24 h and submitted to a shear bonding strength test in a universal testing machine with a crosshead speed of 0.5 mm/min. The failure sites were observed in Scanning Electron Microscopy (SEM). The data were submitted to ANOVA and Tukey's tests (p < 0.05). The mean values (MPa) of shear bond strength were for Fluroshield (25.92 ...

Research paper thumbnail of Photoinitiator chemistry and concentratrion: Effect on polymerization-characteristics and mechanical-properties

Objective: The aim of this study was to determine the influence of photoinitiator chemistry and c... more Objective: The aim of this study was to determine the influence of photoinitiator chemistry and concentration on polymerization characteristics (degree of conversion, crosslink density) and mechanical properties (hardness, elastic modulus) of experimental composites photocured by different methods. Methods: Composites based on BisGMA/TEGDMA and 65% of feldspar filler were prepared with different type and concentration of photoinitiators (camphorquinone (CQ), phenylpropanedione (PPD), bisacylphosphine oxide (BAPO) or CQ/PPD, CQ/BAPO at concentrations of 1% or 2%). Bar specimens were prepared (n=10) and all tests were conducted at the same specimens. Degree of conversion (DC) was determined by Fourier Transform Infrared Spectroscopy (FTIR). Knoop Hardness (KHN)was assessed using un indenter Shimadzu HMV with load of 50g for 5s. Elastic modulus (E) was evaluated through a three point bending flexural test in a Universal testing machine. An indirect evaluation of crosslink density (CLD)...

Research paper thumbnail of Atuação interdisciplinar odontologia/fonoaudiologia no tratamento de paciente com cárie precoce da infância

Revista CEFAC, 2015

ABSTRACT

Research paper thumbnail of Microtensile bond strength of indirect composite restorations using different combinations of resin coating technique

Brazilian Dental Science, 2012

Research paper thumbnail of Atraumatic restorative treatment in Brazilian schoolchildren: 12 months preliminary clinical results

Brazilian Dental Science, 2014

Research paper thumbnail of Comparative Analysis of Root Dentin Morphology and Structure of Human Versus Bovine Primary Teeth

Microscopy and Microanalysis, 2015

This study evaluated the structural and morphological differences between human and bovine primar... more This study evaluated the structural and morphological differences between human and bovine primary root canals. Primary human maxillary central incisors (H) (n=9) and primary bovine incisors (B) (n=9) were selected. The roots were sectioned in the vestibular-lingual direction, planed and delimited in cervical, middle, and apical thirds. Tubule density (number of tubules per mm2) and diameter were analyzed by scanning electron microscopy (1,000 and 5,000×) using Image J 1.47 software. Data were submitted to two-way repeated measures ANOVA and Tukey tests (α=0.05). The highest tubule density was observed for B (28.527±1.717 mm2) compared with H (15.931±0.170 mm2) (p&amp;amp;amp;amp;lt;0.01). Regarding root thirds, the cervical third presented a greater tubule density (26.417±11.654 mm2) than the apical third (17.999±5.873 mm2). The diameter of the dentin tubules was not different for cervical (3.50±0.08 µm), middle (3.45±0.30 µm) and apical thirds (3.42±0.33 µm) and substrate (H-3.29±0.14 µm; B-3.63±0.06 µm). It could be concluded that: (1) the radicular dentin structure of human and bovine primary teeth and root thirds differ in terms of the tubule density; (2) the radicular dentin morphology of human and bovine primary teeth and root thirds are similar in terms of the diameter of the dentin tubules.

Research paper thumbnail of Effect of cleansers and irrigation methods on primary root dentin permeability

Journal of dentistry for children (Chicago, Ill.)

The purpose of this study was to evaluate the effects of cleansers and irrigation methods on the ... more The purpose of this study was to evaluate the effects of cleansers and irrigation methods on the permeability index (PI) in primary root dentin. One hundred twelve teeth were extracted and sectioned transversely, discarding their crowns. Then, the roots were distributed according to the cleansers: (1) Dakin (D); (2) Dakin+hydrogen peroxide (DHP); (3) 2% chlorhexidine gel (C); and (4) saline (S). The canals were manually instrumented. Irrigation was performed manually (MI) or ultrasonically (UI). The roots were: (1) made impermeable; (2) filled with 2% methylene blue solution; and (3) longitudinally sectioned. The halves were marked in thirds (cervical, middle, and apical), and the areas of dye penetration were measured. PI data were submitted to factorial analysis of variance and Tukey tests (P<.05). A significant effect of MI/UI, in association with cleansers, was seen on PI. For all thirds, the PI of MI was superior to UI. In the cervical third, MI achieved a high PI when assoc...

Research paper thumbnail of Effects of water storage on bond strength and dentin sealing ability promoted by adhesive systems

The journal of adhesive dentistry, 2012

To evaluate the dentin bond strength (BS) and sealing ability (SA) promoted by adhesive systems a... more To evaluate the dentin bond strength (BS) and sealing ability (SA) promoted by adhesive systems after 24 h or 6 months of water storage. The tested adhesive systems were: one three-step etch-and-rinse adhesive (Adper Scotchbond Multi-Purpose, SBMP) and three single-step self-etching systems (Adper Easy Bond, Bond Force, and G-Bond Plus). Bovine incisors were used for both evaluations, BS (n = 11) and SA (n = 5). To examine BS, the buccal surface was ground with SiC paper to expose a flat dentin surface. After adhesive application, a block of resin composite was incrementally built up over the bonded surface and sectioned into sticks. These bonded specimens were subjected to microtensile bond strength testing after 24 h and 6 months of water storage using a universal testing machine. For SA analysis, enamel was removed from the buccal surfaces. The teeth were connected to a device to measure the initial SA (10 psi), and the second measurement was taken after treating dentin with EDTA...

Research paper thumbnail of Permeability and smear layer removal: effects of different chemical agents on the primary root dentin

Pediatric dentistry

The purpose of this study was to evaluate the effects of chemical agents on the permeability and ... more The purpose of this study was to evaluate the effects of chemical agents on the permeability and removal of the smear layer in the primary root dentin. Fifty roots were distributed into 6 groups: (1) 5.25% sodium hypochlorite (SH); (2) 5.25% SH+17% ethylenedia-minetetraacetic acid (SHE); (3) 2% chlorhexidine (CHX); (4) 2% CHX+17% ethylenediaminetetraacetic acid (CHXE); (5) 17% EDTA (E); and (6) saline solution (SS). The canals were instrumented and irrigated. The roots were dyed, washed, and sectioned, and the permeability index was measured. The amount of smear layer was scored. Data were submitted to analysis of variance, Kruskal-Wallis, Student-Newman-Keuls and t tests (P<.05). Significant differences were observed in the cervical and apical thirds for the CHX (P=.03), CHXE (P=.027), SS (P=.038), and SH (P<.001) groups. The SS group showed a heavy smear layer in all thirds. Root canals treated with SHE showed great cleaning in the cervical, middle, and apical thirds. Sodium...

Research paper thumbnail of One-year clinical evaluation of oral rehabilitation after the loss of multiple primary teeth

General dentistry

This article describes a one-year follow-up of the prosthetic rehabilitation of a child who lost ... more This article describes a one-year follow-up of the prosthetic rehabilitation of a child who lost multiple primary teeth due to widespread dental caries. Clinical and radiographic examinations indicated the need for all primary teeth to be extracted. Following the extractions, complete maxillary and mandibular removable prostheses with individualized occlusion and age-appropriate artificial teeth were fitted for functional and esthetic rehabilitation. The treatment improved the patient's speech, masticatory function, and facial esthetics, contributing to the development of normal dietary habits and social integration.

Research paper thumbnail of Effects of acidic primer/adhesives on primary and permanent dentin

American journal of dentistry, 2009

To evaluate the quality of primary and permanent dentin by Fourier transformed Raman spectroscopy... more To evaluate the quality of primary and permanent dentin by Fourier transformed Raman spectroscopy (FT-Raman), and scanning electron microscopy/energy-dispersive spectroscopy (SEM/EDS). The middle dentin of crowns was reached by carbide bur abrading providing a uniform smear layer. Self-etching primers were applied in order to simulate the etching of self-etching adhesive systems. The groups were (n = 6): G1 (primary dentin smear layer); G2 (primary dentin etched by primer of Clearfil Protect Bond); G3 (primary dentin etched by Adper Prompt); G4 (permanent dentin smear layer); G5 (permanent dentin etched by primer of Clearfil Protect Bond); G6 (permanent dentin etched by Adper Prompt). SEM/EDS were made in order to obtain additional elemental data to complement FT-Raman. FT-Raman data were submitted to cluster analysis. Overall, FT-Raman showed differences between primary and permanent dentin concerning organic content, but not for inorganic content. FT-Raman showed differences in th...

Research paper thumbnail of Marginal adaptation of pit and fissure sealants after thermal and chemical stress. A SEM study

American journal of dentistry, 2008

To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer m... more To evaluate the in vitro marginal adaptation (gap formation) in the fissure of different sealer materials (resin sealant, glass-ionomer cement, resin-modified glass-ionomer cement, and adhesive system) submitted to thermal and chemical stress, using scanning electron microscopy evaluation (SEM). 80 impacted human third molars were randomly assigned to the following experimental groups (n = 10): FluroShield (F), Helioseal Clear Chroma (H), Vitremer (V), Fuji II-LC (FII), Ketac-Molar (KM), Fuji IX (FIX), Single Bond (SB), and Clearfil Protect Bond (CF). All groups were subjected to thermocycling and 14 days of pH cycling. A blinded and calibrated examiner performed SEM analysis. Gap formation was scored according to: 0 = no sealant marginal gaps; 1 = sealant marginal gaps present or total sealant loss. The score 0 was considered a success, while score 1 represented failure. Data were analyzed by Kruskal-Wallis and Bonferroni tests (P < 0.05). The success rates of SB (100%) and V (9...

Research paper thumbnail of Is the presence of the smear layer a limiting factor for root dentin permeability in primary teeth?

Journal of dentistry for children (Chicago, Ill.)

The purpose of this study was to correlate the dye permeability to the morphological aspect (pres... more The purpose of this study was to correlate the dye permeability to the morphological aspect (presence or absence of a smear layer) of the primary root dentin wall, using scanning electron microscopy, regarding the endodontic preparation and irrigation methods. The hypothesis evaluated was that there was a correlation between the dye permeability and the morphological aspect of the primary root dentin. A total of 112 extracted primary roots were distributed into the following groups: Dakin's liquid, Dakin+hydrogen peroxide; 2% chlorhexidine gel; and saline solution. Manual (MI) or Ultrasonic irrigation (UI) was performed. The roots were made impermeable, filled with dye (2% methylene blue), and longitudinally sectioned. The halves were divided in cervical, middle, and apical thirds for dye penetration measurement. The samples were observed under a scanning electron microscope. The data were submitted to linear regression analysis with a dummy variable (P<.05). The data reveale...

Research paper thumbnail of In vitro wear of primary and permanent enamel. Simultaneous erosion and abrasion

American journal of dentistry, 2007

To evaluate the wear differences between primary and permanent enamel caused by the association o... more To evaluate the wear differences between primary and permanent enamel caused by the association of abrasion and erosion. Fragments of 40 human teeth (20 primary and 20 permanent) were cast in acrylic rings, polished to expose a flat enamel area, and evaluated with a 3D profiler. Antagonists were made from primary and permanent molars. The specimens were distributed into four groups (n = 10) according to type of substrate and slurry (neutral and acidic), and cycled 100,000 times in the OHSU oral wear simulator. Specimens were cleaned and re-profiled. Volume loss and maximum depth were determined on the polished specimens. Data were submitted to ANOVA and Tukey's test. The area of the wear facet on the antagonist was measured. Primary enamel showed more wear than permanent enamel, regardless of the slurry type. The acidic environment increased the wear rate compared to the neutral slurry. The association of abrasion and erosion increased the wear rate for the primary teeth only. T...

Research paper thumbnail of The influence of cleansers on the permeability index of primary tooth root dentin

The Journal of clinical pediatric dentistry, 2006

The aim of this study was to evaluate the action of cleansers on the permeability index (PI) in t... more The aim of this study was to evaluate the action of cleansers on the permeability index (PI) in the root canal of primary dentin, by dye penetration into the length of the root canal and deep dentin. The data were submitted to ANOVA, F, and Student t tests (p<0.05). Lengthwise, chlorhexidine 1% and 2% presented significantly higher mean values than Dakin's liquid. Considering depth, in the cervical third, chlorhexidine 1% and 2% mean values were higher than Dakin's liquid+urea peroxide and Dakin's liquid mean values; distilled water mean values were higher than Dakin's liquid. In the middle and apical thirds, chlorhexidine 1% and 2% mean values were significantly the highest.

Research paper thumbnail of In vitro wear evaluation of dental materials in primary teeth

American journal of dentistry, 2006

To evaluate the effect of dental materials on primary enamel wear. Four composites (Filtek Z250, ... more To evaluate the effect of dental materials on primary enamel wear. Four composites (Filtek Z250, Heliomolar, Point 4, SureFil), two compomers (Dyract AP, F2000) and a resin-modified glass-ionomer (Vitremer) were placed into box-shaped preparations in buccal surfaces of primary molars and stored for 24 hours in water at 37 degrees C. The specimens (n = 6) were subjected to abrasive three-body wear against a primary enamel cusp. Material wear, enamel wear (adjacent to the material), and wear on enamel cusps were estimated by profilometry and video-analysis software. Results were compared with ANOVA and Tukey's tests (P < 0.05). Vitremer showed the highest wear (P < 0.05), followed by Dyract AP and F2000, which were not statistically different (P > 0.05). There was no significant difference between Filtek Z250 and Dyract AP (P > 0.05). Filtek Z250, Heliomolar, Point 4 and SureFil, showed the lowest wear values and there was no difference among them (P > 0.05). The hi...