Antonio Bevilacqua | Università degli Studi di Foggia (original) (raw)
Papers by Antonio Bevilacqua
International Journal of Food Science & Technology
Fermentation
In winemaking, the influence of Saccharomyces cerevisiae strains on the aromatic components of wi... more In winemaking, the influence of Saccharomyces cerevisiae strains on the aromatic components of wine is well recognized on a laboratory scale, but few studies deal with the comparison of numerous strains on a pilot scale fermentation. In this scenario, the present work aimed to validate the fermentative behavior of seven wild S. cerevisiae strains on pilot-scale fermentations to evaluate their impact on the aromatic profiles of the resulting wines. The strains, isolated from grapes of different Italian regional varieties, were tested in pilot-scale fermentation trials performed in the cellar in 1 hL of Aglianico grape must. Then, wines were analyzed for their microbiological cell loads, main chemical parameters of enological interest (ethanol, total sugars, fructose, glucose, total and volatile acidity, malic and lactic acids) and volatile aroma profiles by GC/MS/SPME. Seventy-six volatile compounds belonging to six different classes (esters, alcohols, terpenes, aldehydes, acids, and...
Starter Cultures in Food Production
Frontiers in Microbiology, 2022
Foods, 2021
Food preparations, especially those based on animal products, are often accused of being responsi... more Food preparations, especially those based on animal products, are often accused of being responsible for the increase in food-borne infections, contributing to increased pressure on healthcare systems. The risk assessment in agri-food supply chains is of utmost importance for the food industry and for policymakers. A wrong perception of risks may alter the functioning of supply chains; thus, efforts should be devoted to communicating risks in an efficient way. We adopt a multidisciplinary approach to investigate how consumers perceive different food risks. Our analysis shows that planning effective communication strategies is very much important for efficiently informing consumers on food risks. We also comment on potential innovative ways to better organise the supply chains.
Starter Cultures in Food Production, 2017
Ultrasonics Sonochemistry, 2021
Graphical abstract
Application Of Alternative Food-Preservation Technologies To Enhance Food Safety And Stability, 2010
Application Of Alternative Food-Preservation Technologies To Enhance Food Safety And Stability, 2010
Journal of Food Protection, 2006
A new technique for the immobilization of lysozyme onto the surface of polyvinylalcohol films is ... more A new technique for the immobilization of lysozyme onto the surface of polyvinylalcohol films is presented. The active compound was sprayed along with a suitable bonding agent onto the surface of the cross-linked polymeric matrix. Active compound release tests determined the amount of lysozyme immobilized on the film surface. With the use of Micrococcus lysodeikticus, the antimicrobial activity of the films was determined and the results correlated with the amount of immobilized lysozyme. This new technique was effective for immobilizing the enzyme, and the developed films were active against the test microorganism. Results were compared with those obtained with a different immobilizing technique, in which the active compound was bound into the bulk of the polymeric film. As expected, the surface-immobilized lysozyme films have a higher antimicrobial activity than bulk-bound films.
International Journal of Food Microbiology, 2017
Postharvest Biology and Technology, 2008
The influence of both postharvest treatments and film permeability on the quality decay kinetics ... more The influence of both postharvest treatments and film permeability on the quality decay kinetics of freshly processed grapes was addressed. Ethanol, chlorinated water and hot water were tested to reduce microbiological spoilage. The treated grapes were packaged in two polyester-based biodegradable films (NVT1 and NVT2) and the composition of the atmosphere within the packages determined. A multilayer film, made by laminating an aluminium foil with a polyethylene film (aluminium) was used to evaluate separately the respiratory activity of the packed grapes, and the oxygen and carbon dioxide permeability coefficients of the investigated films under real working conditions. The quality decay kinetics of freshly processed table grapes packed at 5°C were assessed by monitoring the cell load of the main spoilage microorganisms (total mesophilic viable count, lactic acid bacteria, yeasts and moulds) and the product appearance for a period of about 30 days. Results suggest that ethanol was the most efficient treatment, as it successfully reduced the cell load of the spoilage microorganisms without affecting respiration of the packed food or its appearance. Differences between the investigated biodegradable films were not statistically significant.
Journal of the Science of Food and Agriculture, 2010
Oak barrels are commonly used in the aging of wines and spirits because of their positive effects... more Oak barrels are commonly used in the aging of wines and spirits because of their positive effects on the product. In recent years the addition of oak chips has been used to introduce desirable wood aromas and flavours into wines. In this study, oak chips in saline solution or laboratory medium were inoculated with Penicillium purpurogenum, Aureobasidium pullulans, Phialemonium obovatum, Phanerochaete chrysosporium and a combination of Ph. chrysosporium and A. pullulans. After 12 weeks of incubation, oak chips (2 g L(-1)) were macerated in a red wine for 17 days. Gas chromatography/mass spectrometry and high-performance liquid chromatography were used to evaluate 14 compounds, namely furfural, furfuryl alcohol, guaiacol, syringol, cis-β-methyl-γ-octalactone, 2-phenylethanol, 4-vinylguaiacol, benzyl alcohol, 2,3-butanediol, γ-butyrolactone, benzaldehyde, 4-ethylguaiacol, gallic acid and ellagic acid. The microfungal treatments increased the concentration of some components. In particular, P. purpurogenum resulted in a significant improvement in the levels of guaiacol, furfural, syringol, furfuryl alcohol and 2-phenylethanol. Penicillium purpurogenum and Ph. chrysosporium showed a constant trend (enrichment of furfural and benzaldehyde) independent to some extent of the medium used for chip treatment.
Journal of the Science of Food and Agriculture, 2011
Journal of Food Engineering, 2009
In this study, the properties of whey protein isolate (WPI)-based films with different levels of ... more In this study, the properties of whey protein isolate (WPI)-based films with different levels of glycerol and spelt bran were investigated using response surface methodology. Tests were carried out to determine to what extent the water vapor permeability (WVP) of the films can be improved by adding the bran. Moreover, the mechanical and dynamical properties, colour of the films and the rheological properties of the film-forming solutions were also investigated. Results highlighted that the WVP was significantly affected by the spelt bran concentration: increasing the spelt bran concentration led to decrease in water vapor permeability, whereas the glycerol concentration incremented the WVP values of the films. The elastic modulus (E C) and complex modulus (E Ã) of the composite films increased with the decrease of the glycerol content and the increase of the bran concentration. Glycerol addition, acting as a plasticizer, led to more flexible films. Regarding the rheological characteristics, the WPI solutions with the lower bran concentration behaved as Newtonian fluid. As the bran concentration increased the solutions demonstrated a non-Newtonian behaviour. Colour results highlighted that yellow index (YI), a and b parameter of Hunter scale increased with the bran concentration increase; on the contrary, L parameter decreased with the bran concentration increase.
Microorganisms
Thymol and carvacrol are some of the most important and used components of Essential oils (EOs); ... more Thymol and carvacrol are some of the most important and used components of Essential oils (EOs); they are widely studied, and there are much data available in the literature. Their Minimal Inhibitory Concentration (MIC) values found in the literature from 2005 to present were used to assess the bioactivity toward yeasts, molds, Gram-positive bacteria, and Gram-negative bacteria, as well as on some bacterial species/serotypes (Salmonella sp., Escherichia coli, E. coli O157:H7, lactic acid bacteria, Listeria monocytogenes, Staphylococcus aureus, S. epidermidis, etc.) to find possible common trends or differences between the two compounds and among the tested species. The results were quite interesting and pointed out that there is a common range for the MIC of thymol and carvacrol for some bacterial species (150–400 mg/L), with some exceptions to this generalized statement. In addition, the statistics pointed out that bacteria could experience homogeneous (S. epidermidis, E. coli O157...
Frontiers in Microbiology
The main goal of this research was to study the effect of an Ultrasound (US) treatment on biofilm... more The main goal of this research was to study the effect of an Ultrasound (US) treatment on biofilm formation of Lactiplantibacillus plantarum (strains c19 and DSM 1055), Bifidobacterium animalis subsp. lactis DSM 10140, Bifidobacterium longum subsp. longum DSM 20219, and Bifidobacterium longum subsp. infantis DSM 20088. From a methodological point of view, each microorganism was treated through six US treatments, different for the power (10, 30, or 50% of the net power, 130 W), the duration (2, 6, or 10 min) and the application of pulses (0 or 10 s). After the treatment, a biofilm of the strains was let to form on glass slides and the concentration of sessile cells was analyzed for 16 days. Biofilms formed by untreated microorganisms were used as controls. As a first result, it was found that US significantly increased the concentration of sessile cells of B. longum subsp. infantis, while for some other strains US treatment could not affect the formation of biofilm while improving it...
Microorganisms
The main goal of this paper was to assess the ability of a combination of Candida boidinii and Ba... more The main goal of this paper was to assess the ability of a combination of Candida boidinii and Bacillus pumilus to remove phenol in table olive processing water, as a function of some variables, like temperature, pH, a dilution of waste and the order of inoculation of the two microorganisms. At this purpose C. boidinii and B. pumilus were sequentially inoculated in two types of table olive processing water (fresh wastewater, FTOPW and wastewater stored for 3 months-aged wastewater, ATOPW). pH (6 and 9), temperature (10 and 35 °C) and dilution ratio (0, 1:1) were combined through a 2k fractional design. Data were modeled using two different approaches: Multifactorial Analysis of Variance (MANOVA) and multiple regression. A higher removal yield was achieved by inoculating B. pumilus prior to the yeast (192 vs. 127 mg/L); moreover, an increased efficiency was gained at 35 °C (mean removal of 200 mg/L). The use of two statistic approach suggested a different weight of variables; tempera...
Nutrients
Many scientific studies reveal a significant connection between human intestinal microbiota, eati... more Many scientific studies reveal a significant connection between human intestinal microbiota, eating habits, and the development of chronic-degenerative diseases; therefore, alterations in the composition and function of the microbiota may be accompanied by different chronic inflammatory mechanisms. Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS), in which autoreactive immune cells attack the myelin sheaths of the neurons. The purpose of this paper was to describe the main changes that occur in the gut microbiota of MS patients, with a focus on both microbiota and its implications for health and disease, as well as the variables that influence it. Another point stressed by this paper is the role of microbiota as a triggering factor to modulate the responses of the innate and adaptive immune systems, both in the intestine and in the brain. In addition, a comprehensive overview of the taxa modified by the disease is presented, with s...
Microorganisms
Probiotics are gradually gaining importance in the field of psychiatry in the form of psychobioti... more Probiotics are gradually gaining importance in the field of psychiatry in the form of psychobiotics. Psychobiotics’ studies examine the existing relationship between gut microbiota and mental phenomena; the intake of certain strains of probiotics, such as Bifidobacterium and Lactobacillus, for example, allow the gut microbial system to be modified in order to provide benefits at the psychic, immune, hormonal, and mental levels. Those who suffer from forms of depression, anxiety disorders, chronic stress, low mood, but also people who do not suffer from such disorders, can therefore benefit from the use of psychobiotics. Thanks to probiotics, neurochemicals can in fact be produced within the gut microbiota and interact with receptors of the enteric nervous system that innervate the entire gastrointestinal tract. Once they enter the portal circulation, these substances go on to influence components of the nervous system and ultimately the brain, through what is called the gut–brain ax...
International Journal of Food Science & Technology
Fermentation
In winemaking, the influence of Saccharomyces cerevisiae strains on the aromatic components of wi... more In winemaking, the influence of Saccharomyces cerevisiae strains on the aromatic components of wine is well recognized on a laboratory scale, but few studies deal with the comparison of numerous strains on a pilot scale fermentation. In this scenario, the present work aimed to validate the fermentative behavior of seven wild S. cerevisiae strains on pilot-scale fermentations to evaluate their impact on the aromatic profiles of the resulting wines. The strains, isolated from grapes of different Italian regional varieties, were tested in pilot-scale fermentation trials performed in the cellar in 1 hL of Aglianico grape must. Then, wines were analyzed for their microbiological cell loads, main chemical parameters of enological interest (ethanol, total sugars, fructose, glucose, total and volatile acidity, malic and lactic acids) and volatile aroma profiles by GC/MS/SPME. Seventy-six volatile compounds belonging to six different classes (esters, alcohols, terpenes, aldehydes, acids, and...
Starter Cultures in Food Production
Frontiers in Microbiology, 2022
Foods, 2021
Food preparations, especially those based on animal products, are often accused of being responsi... more Food preparations, especially those based on animal products, are often accused of being responsible for the increase in food-borne infections, contributing to increased pressure on healthcare systems. The risk assessment in agri-food supply chains is of utmost importance for the food industry and for policymakers. A wrong perception of risks may alter the functioning of supply chains; thus, efforts should be devoted to communicating risks in an efficient way. We adopt a multidisciplinary approach to investigate how consumers perceive different food risks. Our analysis shows that planning effective communication strategies is very much important for efficiently informing consumers on food risks. We also comment on potential innovative ways to better organise the supply chains.
Starter Cultures in Food Production, 2017
Ultrasonics Sonochemistry, 2021
Graphical abstract
Application Of Alternative Food-Preservation Technologies To Enhance Food Safety And Stability, 2010
Application Of Alternative Food-Preservation Technologies To Enhance Food Safety And Stability, 2010
Journal of Food Protection, 2006
A new technique for the immobilization of lysozyme onto the surface of polyvinylalcohol films is ... more A new technique for the immobilization of lysozyme onto the surface of polyvinylalcohol films is presented. The active compound was sprayed along with a suitable bonding agent onto the surface of the cross-linked polymeric matrix. Active compound release tests determined the amount of lysozyme immobilized on the film surface. With the use of Micrococcus lysodeikticus, the antimicrobial activity of the films was determined and the results correlated with the amount of immobilized lysozyme. This new technique was effective for immobilizing the enzyme, and the developed films were active against the test microorganism. Results were compared with those obtained with a different immobilizing technique, in which the active compound was bound into the bulk of the polymeric film. As expected, the surface-immobilized lysozyme films have a higher antimicrobial activity than bulk-bound films.
International Journal of Food Microbiology, 2017
Postharvest Biology and Technology, 2008
The influence of both postharvest treatments and film permeability on the quality decay kinetics ... more The influence of both postharvest treatments and film permeability on the quality decay kinetics of freshly processed grapes was addressed. Ethanol, chlorinated water and hot water were tested to reduce microbiological spoilage. The treated grapes were packaged in two polyester-based biodegradable films (NVT1 and NVT2) and the composition of the atmosphere within the packages determined. A multilayer film, made by laminating an aluminium foil with a polyethylene film (aluminium) was used to evaluate separately the respiratory activity of the packed grapes, and the oxygen and carbon dioxide permeability coefficients of the investigated films under real working conditions. The quality decay kinetics of freshly processed table grapes packed at 5°C were assessed by monitoring the cell load of the main spoilage microorganisms (total mesophilic viable count, lactic acid bacteria, yeasts and moulds) and the product appearance for a period of about 30 days. Results suggest that ethanol was the most efficient treatment, as it successfully reduced the cell load of the spoilage microorganisms without affecting respiration of the packed food or its appearance. Differences between the investigated biodegradable films were not statistically significant.
Journal of the Science of Food and Agriculture, 2010
Oak barrels are commonly used in the aging of wines and spirits because of their positive effects... more Oak barrels are commonly used in the aging of wines and spirits because of their positive effects on the product. In recent years the addition of oak chips has been used to introduce desirable wood aromas and flavours into wines. In this study, oak chips in saline solution or laboratory medium were inoculated with Penicillium purpurogenum, Aureobasidium pullulans, Phialemonium obovatum, Phanerochaete chrysosporium and a combination of Ph. chrysosporium and A. pullulans. After 12 weeks of incubation, oak chips (2 g L(-1)) were macerated in a red wine for 17 days. Gas chromatography/mass spectrometry and high-performance liquid chromatography were used to evaluate 14 compounds, namely furfural, furfuryl alcohol, guaiacol, syringol, cis-β-methyl-γ-octalactone, 2-phenylethanol, 4-vinylguaiacol, benzyl alcohol, 2,3-butanediol, γ-butyrolactone, benzaldehyde, 4-ethylguaiacol, gallic acid and ellagic acid. The microfungal treatments increased the concentration of some components. In particular, P. purpurogenum resulted in a significant improvement in the levels of guaiacol, furfural, syringol, furfuryl alcohol and 2-phenylethanol. Penicillium purpurogenum and Ph. chrysosporium showed a constant trend (enrichment of furfural and benzaldehyde) independent to some extent of the medium used for chip treatment.
Journal of the Science of Food and Agriculture, 2011
Journal of Food Engineering, 2009
In this study, the properties of whey protein isolate (WPI)-based films with different levels of ... more In this study, the properties of whey protein isolate (WPI)-based films with different levels of glycerol and spelt bran were investigated using response surface methodology. Tests were carried out to determine to what extent the water vapor permeability (WVP) of the films can be improved by adding the bran. Moreover, the mechanical and dynamical properties, colour of the films and the rheological properties of the film-forming solutions were also investigated. Results highlighted that the WVP was significantly affected by the spelt bran concentration: increasing the spelt bran concentration led to decrease in water vapor permeability, whereas the glycerol concentration incremented the WVP values of the films. The elastic modulus (E C) and complex modulus (E Ã) of the composite films increased with the decrease of the glycerol content and the increase of the bran concentration. Glycerol addition, acting as a plasticizer, led to more flexible films. Regarding the rheological characteristics, the WPI solutions with the lower bran concentration behaved as Newtonian fluid. As the bran concentration increased the solutions demonstrated a non-Newtonian behaviour. Colour results highlighted that yellow index (YI), a and b parameter of Hunter scale increased with the bran concentration increase; on the contrary, L parameter decreased with the bran concentration increase.
Microorganisms
Thymol and carvacrol are some of the most important and used components of Essential oils (EOs); ... more Thymol and carvacrol are some of the most important and used components of Essential oils (EOs); they are widely studied, and there are much data available in the literature. Their Minimal Inhibitory Concentration (MIC) values found in the literature from 2005 to present were used to assess the bioactivity toward yeasts, molds, Gram-positive bacteria, and Gram-negative bacteria, as well as on some bacterial species/serotypes (Salmonella sp., Escherichia coli, E. coli O157:H7, lactic acid bacteria, Listeria monocytogenes, Staphylococcus aureus, S. epidermidis, etc.) to find possible common trends or differences between the two compounds and among the tested species. The results were quite interesting and pointed out that there is a common range for the MIC of thymol and carvacrol for some bacterial species (150–400 mg/L), with some exceptions to this generalized statement. In addition, the statistics pointed out that bacteria could experience homogeneous (S. epidermidis, E. coli O157...
Frontiers in Microbiology
The main goal of this research was to study the effect of an Ultrasound (US) treatment on biofilm... more The main goal of this research was to study the effect of an Ultrasound (US) treatment on biofilm formation of Lactiplantibacillus plantarum (strains c19 and DSM 1055), Bifidobacterium animalis subsp. lactis DSM 10140, Bifidobacterium longum subsp. longum DSM 20219, and Bifidobacterium longum subsp. infantis DSM 20088. From a methodological point of view, each microorganism was treated through six US treatments, different for the power (10, 30, or 50% of the net power, 130 W), the duration (2, 6, or 10 min) and the application of pulses (0 or 10 s). After the treatment, a biofilm of the strains was let to form on glass slides and the concentration of sessile cells was analyzed for 16 days. Biofilms formed by untreated microorganisms were used as controls. As a first result, it was found that US significantly increased the concentration of sessile cells of B. longum subsp. infantis, while for some other strains US treatment could not affect the formation of biofilm while improving it...
Microorganisms
The main goal of this paper was to assess the ability of a combination of Candida boidinii and Ba... more The main goal of this paper was to assess the ability of a combination of Candida boidinii and Bacillus pumilus to remove phenol in table olive processing water, as a function of some variables, like temperature, pH, a dilution of waste and the order of inoculation of the two microorganisms. At this purpose C. boidinii and B. pumilus were sequentially inoculated in two types of table olive processing water (fresh wastewater, FTOPW and wastewater stored for 3 months-aged wastewater, ATOPW). pH (6 and 9), temperature (10 and 35 °C) and dilution ratio (0, 1:1) were combined through a 2k fractional design. Data were modeled using two different approaches: Multifactorial Analysis of Variance (MANOVA) and multiple regression. A higher removal yield was achieved by inoculating B. pumilus prior to the yeast (192 vs. 127 mg/L); moreover, an increased efficiency was gained at 35 °C (mean removal of 200 mg/L). The use of two statistic approach suggested a different weight of variables; tempera...
Nutrients
Many scientific studies reveal a significant connection between human intestinal microbiota, eati... more Many scientific studies reveal a significant connection between human intestinal microbiota, eating habits, and the development of chronic-degenerative diseases; therefore, alterations in the composition and function of the microbiota may be accompanied by different chronic inflammatory mechanisms. Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS), in which autoreactive immune cells attack the myelin sheaths of the neurons. The purpose of this paper was to describe the main changes that occur in the gut microbiota of MS patients, with a focus on both microbiota and its implications for health and disease, as well as the variables that influence it. Another point stressed by this paper is the role of microbiota as a triggering factor to modulate the responses of the innate and adaptive immune systems, both in the intestine and in the brain. In addition, a comprehensive overview of the taxa modified by the disease is presented, with s...
Microorganisms
Probiotics are gradually gaining importance in the field of psychiatry in the form of psychobioti... more Probiotics are gradually gaining importance in the field of psychiatry in the form of psychobiotics. Psychobiotics’ studies examine the existing relationship between gut microbiota and mental phenomena; the intake of certain strains of probiotics, such as Bifidobacterium and Lactobacillus, for example, allow the gut microbial system to be modified in order to provide benefits at the psychic, immune, hormonal, and mental levels. Those who suffer from forms of depression, anxiety disorders, chronic stress, low mood, but also people who do not suffer from such disorders, can therefore benefit from the use of psychobiotics. Thanks to probiotics, neurochemicals can in fact be produced within the gut microbiota and interact with receptors of the enteric nervous system that innervate the entire gastrointestinal tract. Once they enter the portal circulation, these substances go on to influence components of the nervous system and ultimately the brain, through what is called the gut–brain ax...