Luis Terán | University of Fribourg (original) (raw)

Papers by Luis Terán

Research paper thumbnail of Public Collaborative Legislation A Case Study of the Ingenios Act

—Collaborative working environments have commonly been used by companies which also use informati... more —Collaborative working environments have commonly been used by companies which also use information and communication technologies that allow synchronous and asynchronous real time interactions between individuals who share common objectives and work. These strategies have proven to be very effective for the private sector and can be adopted by public administration to encourage participation from citizens and eventually result in empowerment of societies. In this paper, a case study on public collaborative legislation, termed Wiki Legislation, is used for the analysis and development of an Act. The Act generated during the implementation of this project aims to integrate the systems of Science, Technology, Innovation and Ancestral Knowledge with Education, Higher Education and Culture in Ecuador. In this work, the design of the project, as well as a user-centered evaluation was conducted. Recommendations on how to enhance the development of future collaborative legislation projects are provided.

Research paper thumbnail of Improved method for isolation of porcine neonatal pancreatic cell clusters

Xenotransplantation, 2005

Based on the described methods for the isolation of neonatal pancreatic cell clusters (NPCCs), we... more Based on the described methods for the isolation of neonatal pancreatic cell clusters (NPCCs), we have developed modifications in order to improve their quality, functionality, and process reproducibility in the isolation technique, for potential use in research. In addition, we indicate techniques for describing yield, functionality, viability and purity of the NPCCs. Purity of the NPCCs was determined through dithizone staining and subjected to image analysis. Viability and apoptosis was measured through flow cytometry with propidium iodide and annexin, respectively. NPCC functionality was measured through a static glucose stimulation test. We developed a high-yield reproducible technique that had 81 279.55 +/- 18 257.05 IEQ/g of pancreas at 4 days of culture, with a 94% viability and an 88 +/- 2.73% purity. Stimulation index from the glucose stimulation test was >10. The technique allowed us to obtain NPCC with optimal viability, functionality, purity, and endurance for use in research.

Research paper thumbnail of CCL Chemokines and asthma

Immunology Today, 2000

Airway eosinophilia is a characteristic of bronchial asthma. Eosinophils are considered to cause ... more Airway eosinophilia is a characteristic of bronchial asthma. Eosinophils are considered to cause tissue damage through the release of toxic proteases, lipid mediators, cytokines and oxygen free radicals. The discovery of chemokines and the demonstration that some members of this cytokine superfamily are implicated in the recruitment of eosinophils offers an opportunity for a novel therapeutic approach in asthma.

Research paper thumbnail of Inside the Outbreak of the 2009 Influenza A (H1N1)v Virus in Mexico

Research paper thumbnail of 72-kD (MMP-2) and 92-kD (MMP-9) Type IV Collagenase Production and Activity in Different Histologic Types of Lung Cancer Cells

Pathobiology, 1998

In this study we examined the production of gelatinases A and B (MMP-2 and MMP-9), and their natu... more In this study we examined the production of gelatinases A and B (MMP-2 and MMP-9), and their natural inhibitors TIMP-1 and TIMP-2 in cell lines derived from different histologic types of lung cancer. Gelatinolytic activity was measured by zymography and radiolabeled gelatin degradation. Immunocytochemistry and Western blot analysis were performed to corroborate the presence of immunoreactive MMP-2, MMP-9, TIMP-1 and TIMP-2 proteins. The highest gelatinolytic activity was identified in the cell extracts from a small-cell carcinoma cell line. MMP-9 was observed in all samples as a proenzyme, while MMP-2 was present as zymogen in the squamous-cell and in the small-cell carcinomas, and in its active form in one squamous-cell carcinoma cell line. TIMPs were also present in the neoplastic lung cell lines. TIMP-1 was observed in the media of all cells as a 21-kD band, and as TIMP-1 polymers with the exception of the small-cell carcinoma samples. TIMP-2 was found as higher-order molecular immunoreactive complexes that may correspond to proMMP-2/TIMP-2 complexes. These results demonstrate that lung neoplastic cells produce both MMP-2 and MMP-9 and their inhibitors, with the small-cell carcinoma cell extracts showing the highest enzymatic activity. This gelatinolytic activity fits well with the clinical metastatic behavior of this type of lung cancer.

Research paper thumbnail of An anti-inflammatory oligopeptide produced by Entamoeba histolytica down-regulates the expression of pro-inflammatory chemokines

Parasite Immunology, 2003

Research paper thumbnail of Influence on Collagen Metabolism of Vitreous from Eyes with Proliferative Vitreoretinopathy

Research paper thumbnail of Unexpected Immunoresponse to Gal and APA Antigens in Diabetic Type 1 Patients Receiving Neonatal Pig Islets After 6 Years

Journal of Clinical Immunology, 2007

Research paper thumbnail of 861 Th1 and Th2 type cytokines regulate chemokine production from human airway epithelial cells

Journal of Allergy and Clinical Immunology, 2000

Research paper thumbnail of Cellular and mediator responses twenty-four hours after local endobronchial allergen challenge of asthmatic airways

Journal of Allergy and Clinical Immunology, 1996

The effects of acute allergen exposure on bronchoalveolar lavage cells and mediators and mucosal ... more The effects of acute allergen exposure on bronchoalveolar lavage cells and mediators and mucosal inflammatory cells were evaluated in 10 subjects with atopic asthma who underwent lavage and biopsy 24 hours after segmental endobronchial allergen challenge. Increased numbers of bronchoalveolar lavage eosinophils were retrieved from the allergen-challenged sites compared with the saline-challenged sites (mean 21.4 vs 1.5 x 10(3) cells/ml; p < 0.02). Numbers of neutrophils and proportions of CD4+, CD8+, CD25+, and HLA-DR+ T cells were similar at the saline- and allergen-challenged sites. In contrast to the bronchoalveolar lavage findings, eosinophil numbers were not increased in the bronchial submucosa or epithelium. There was also no significant difference in neutrophils, mast cells, CD3+, CD4+, or CD8+ T cells in the submucosa after allergen challenge, but the number of activated (CD25+) T lymphocytes in the mucosa did increase after allergen challenge. Allergen challenge did not induce any significant change in endothelial expression of P-selectin, E-selectin, intercellular adhesion molecule-1, or vascular cell adhesion molecule-1. CD11a+ and very late antigen-4+ cell numbers were similar in the saline- and allergen-challenged sites. This study suggests that in patients with very mild asthma, local allergen challenge induces persistent bronchoalveolar lavage eosinophilia, but the recruitment process seems to have diminished or ceased by 24 hours.

Research paper thumbnail of Cultured nasal polyps from nonatopic and atopic patients release RANTES spontaneously and after stimulation with phytohemagglutinin☆☆☆★★★

Journal of Allergy and Clinical Immunology, 1997

Eosinophil infiltration of tissue is a hallmark of nasal polyposis in both nonatopic and atopic p... more Eosinophil infiltration of tissue is a hallmark of nasal polyposis in both nonatopic and atopic patients. These cells are thought to play a key role in the nasal polyp inflammatory process. The objective of this study was to investigate whether cultured nasal polyps derived from nonatopic and atopic patients release RANTES both spontaneously and after phytohemagglutinin (PHA) stimulation. Nasal polyps were obtained from 12 subjects (6 nonatopic and 6 atopic), cut into 2 to 3 mm large specimens, and cultured for 48 hours with or without PHA. RANTES was measured in the culture supernatant by ELISA (R&D Systems, U.K.). Immunoreactive RANTES was found to be present in the culture supernatant of nasal polyps derived from both nonatopic and atopic patients with no difference between the two groups (median: 3.8 vs 2.9 pg/mg/ml). On incubation with PHA, nasal polyps from both nonatopic and atopic patients released sevenfold and 11-fold greater amounts of RANTES than unstimulated samples. As determined by immunohistochemistry, RANTES was localized to the vascular endothelium in nasal polyps from both groups of patients. This study demonstrates that cultured nasal polyps derived from both nonatopic and atopic patients release RANTES spontaneously and after PHA stimulation. This observation and the finding that RANTES is present in nasal polyp endothelial cells suggest that this chemokine may be an important mediator of eosinophil and lymphocyte recruitment in both nonatopic and atopic nasal polyposis.

Research paper thumbnail of The chemokines RANTES, MCP-3, MCP-4, eotaxin-1 and eotaxin-2 are associated with eosinophil recruitment into the airways of asthmatic children

Journal of Allergy and Clinical Immunology, 2002

The Journal of Allergy and Clinical Immunology, Volume 109, Issue 1, Pages S36, January 2002, Aut... more The Journal of Allergy and Clinical Immunology, Volume 109, Issue 1, Pages S36, January 2002, Authors:Luis M Teran; Enrique Rojas-Ramos; Ana Francisca Avalos; Lorenzo Perez-Fernandez; Francisco Cuevas. ...

Research paper thumbnail of Th2- and to a Lesser Extent Th1-Type Cytokines Upregulate the Production of both CXC (IL-8 and Gro-Alpha) and CC (RANTES, Eotaxin, Eotaxin-2, MCP-3 and MCP-4) Chemokines in Human Airway Epithelial Cells

International Archives of Allergy and Immunology, 2003

Both CXC and CC chemokines play an important role in leukocyte recruitment. However, a systematic... more Both CXC and CC chemokines play an important role in leukocyte recruitment. However, a systematic examination of their production by human airway epithelial cells (HAECs) has not been carried out. The objective of this study was to investigate whether Th1- and Th2-type cytokines regulate chemokine production in HAECs. HAECs were grown from both nasal and bronchial tissue and subsequently stimulated with either Th1- or Th2-type cytokines. Constitutive mRNA expression for gro-alpha, IL-8 and RANTES was seen in both human nasal and human bronchial epithelial cells. IL-4 was the strongest stimulus for both gene expression and protein production of the chemokines RANTES, IL-8 and gro-alpha, while both IL-13 and IFN-gamma were weaker inducers of these chemokines, with the exception of gro-alpha (IL-13 was a strong stimulus for gro-alpha production). TNF-alpha synergized with IL-4, and to a lesser extent with IFN-gamma and IL-13, to release RANTES, IL-8 and gro-alpha. IL-4 and to a lesser extent IL-13 and IFN-gamma stimulated the production of MCP-3 and -4, eotaxin and eotaxin-2 immunoreactivities. However, no induction of the mRNAs encoding these chemokines was observed, suggesting that they may be released from a preformed pool within the HAECs. These findings suggest that when released into the airways, Th2- and to a lesser extent Th1-type cytokines may stimulate recruitment of eosinophils and neutrophils through the release of CC (RANTES, MCP-3 and -4, eotaxin and eotaxin-2) and CXC chemokines (gro-alpha and IL-8).

Research paper thumbnail of RANTES Production by Cytokine–Stimulated Nasal Fibroblasts: Its Inhibition by Glucocorticoids

International Archives of Allergy and Immunology, 1998

Nasal fibroblasts play an important role in both nasal polyposis and nasal allergic diseases and ... more Nasal fibroblasts play an important role in both nasal polyposis and nasal allergic diseases and they are known to release a number of proinflammatory cytokines, including GM–CSF, IL–8 and IL–6. The aim of this present work was to investigate whether cytokine–stimulated nasal fibroblasts release biologically active RANTES as well as to study the effect of corticosteroids on the ability of

Research paper thumbnail of Granulocyte Recruitment by Human Mast Cell Tryptase

International Archives of Allergy and Immunology, 1995

... Eosinophil; Epithelial cells; Mast cell; Neutrophil; Tryptase. goto top of page Author Contac... more ... Eosinophil; Epithelial cells; Mast cell; Neutrophil; Tryptase. goto top of page Author Contacts. Correspondence to: Dr. Andrew F. Walls, University Medicine, Level F, Southampton General Hospital, Tremona Road, Southampton SO16 6YD (UK). goto top of page Article Information ...

Research paper thumbnail of Neutrophil Influx and lnterleukin-8 Release after Segmental Allergen or Saline Challenge in Asthmatics

International Archives of Allergy and Immunology, 1995

Neutrophils have been associated with the late asthmatic reaction. However, their role in this di... more Neutrophils have been associated with the late asthmatic reaction. However, their role in this disease is not well understood. In this study, we have measured neutrophil influx and release of interleukin-8 into asthmatic airways after endobronchial challenge with either allergen or saline solution.Copyright © 1995 S. Karger AG, Basel

Research paper thumbnail of Role of monocyte chemotactic protein-3 and -4 in children with virus exacerbation of asthma

European Respiratory Journal, 2008

Research paper thumbnail of Effects of 0.2 ppm ozone on biomarkers of inflammation in bronchoalveolar lavage fluid and bronchial mucosa of healthy subjects

European Respiratory Journal, 1998

Research paper thumbnail of Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma

European Respiratory Journal, 2003

Eosinophil recruitment into the airways is a feature of asthma in children. However, the mechanis... more Eosinophil recruitment into the airways is a feature of asthma in children. However, the mechanisms by which these cells migrate into the airways are not fully understood. The present study investigated the presence of the eosinophil-activating chemokines regulated on activation, normal T-cell expressed and secreted (RANTES), monocyte chemotactic proteins (MCP)-3 and -4, and eotaxins-1 and -2 in the bronchoalveolar lavage (BAL) fluid obtained from both asthmatic (n=10, age 6-10 yrs) and normal children (n=10, age 5-10 yrs). Measurements of chemokines in BAL fluid showed that levels of RANTES, MCPs-3 and -4, and eotaxins-1 and -2 were significantly increased in fluid obtained from asthmatic children when compared with normal children. Among the different chemokines, RANTES was the cytokine released in greatest quantities in BAL fluid from asthmatic children. There was a significant correlation between the concentrations of MCP-4 and eosinophil numbers in BAL fluid and a trend between both chemokines MCP-3 and eotaxin-2 and eosinophils. Interestingly, the levels of most chemokines correlated with one another. These findings suggest that RANTES monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 may regulate eosinophil trafficking into the airways of asthmatic children in a coordinated manner.

Research paper thumbnail of CC chemokine ligand 1 is released into the airways of atopic asthmatics

European Respiratory Journal, 2006

Research paper thumbnail of Public Collaborative Legislation A Case Study of the Ingenios Act

—Collaborative working environments have commonly been used by companies which also use informati... more —Collaborative working environments have commonly been used by companies which also use information and communication technologies that allow synchronous and asynchronous real time interactions between individuals who share common objectives and work. These strategies have proven to be very effective for the private sector and can be adopted by public administration to encourage participation from citizens and eventually result in empowerment of societies. In this paper, a case study on public collaborative legislation, termed Wiki Legislation, is used for the analysis and development of an Act. The Act generated during the implementation of this project aims to integrate the systems of Science, Technology, Innovation and Ancestral Knowledge with Education, Higher Education and Culture in Ecuador. In this work, the design of the project, as well as a user-centered evaluation was conducted. Recommendations on how to enhance the development of future collaborative legislation projects are provided.

Research paper thumbnail of Improved method for isolation of porcine neonatal pancreatic cell clusters

Xenotransplantation, 2005

Based on the described methods for the isolation of neonatal pancreatic cell clusters (NPCCs), we... more Based on the described methods for the isolation of neonatal pancreatic cell clusters (NPCCs), we have developed modifications in order to improve their quality, functionality, and process reproducibility in the isolation technique, for potential use in research. In addition, we indicate techniques for describing yield, functionality, viability and purity of the NPCCs. Purity of the NPCCs was determined through dithizone staining and subjected to image analysis. Viability and apoptosis was measured through flow cytometry with propidium iodide and annexin, respectively. NPCC functionality was measured through a static glucose stimulation test. We developed a high-yield reproducible technique that had 81 279.55 +/- 18 257.05 IEQ/g of pancreas at 4 days of culture, with a 94% viability and an 88 +/- 2.73% purity. Stimulation index from the glucose stimulation test was >10. The technique allowed us to obtain NPCC with optimal viability, functionality, purity, and endurance for use in research.

Research paper thumbnail of CCL Chemokines and asthma

Immunology Today, 2000

Airway eosinophilia is a characteristic of bronchial asthma. Eosinophils are considered to cause ... more Airway eosinophilia is a characteristic of bronchial asthma. Eosinophils are considered to cause tissue damage through the release of toxic proteases, lipid mediators, cytokines and oxygen free radicals. The discovery of chemokines and the demonstration that some members of this cytokine superfamily are implicated in the recruitment of eosinophils offers an opportunity for a novel therapeutic approach in asthma.

Research paper thumbnail of Inside the Outbreak of the 2009 Influenza A (H1N1)v Virus in Mexico

Research paper thumbnail of 72-kD (MMP-2) and 92-kD (MMP-9) Type IV Collagenase Production and Activity in Different Histologic Types of Lung Cancer Cells

Pathobiology, 1998

In this study we examined the production of gelatinases A and B (MMP-2 and MMP-9), and their natu... more In this study we examined the production of gelatinases A and B (MMP-2 and MMP-9), and their natural inhibitors TIMP-1 and TIMP-2 in cell lines derived from different histologic types of lung cancer. Gelatinolytic activity was measured by zymography and radiolabeled gelatin degradation. Immunocytochemistry and Western blot analysis were performed to corroborate the presence of immunoreactive MMP-2, MMP-9, TIMP-1 and TIMP-2 proteins. The highest gelatinolytic activity was identified in the cell extracts from a small-cell carcinoma cell line. MMP-9 was observed in all samples as a proenzyme, while MMP-2 was present as zymogen in the squamous-cell and in the small-cell carcinomas, and in its active form in one squamous-cell carcinoma cell line. TIMPs were also present in the neoplastic lung cell lines. TIMP-1 was observed in the media of all cells as a 21-kD band, and as TIMP-1 polymers with the exception of the small-cell carcinoma samples. TIMP-2 was found as higher-order molecular immunoreactive complexes that may correspond to proMMP-2/TIMP-2 complexes. These results demonstrate that lung neoplastic cells produce both MMP-2 and MMP-9 and their inhibitors, with the small-cell carcinoma cell extracts showing the highest enzymatic activity. This gelatinolytic activity fits well with the clinical metastatic behavior of this type of lung cancer.

Research paper thumbnail of An anti-inflammatory oligopeptide produced by Entamoeba histolytica down-regulates the expression of pro-inflammatory chemokines

Parasite Immunology, 2003

Research paper thumbnail of Influence on Collagen Metabolism of Vitreous from Eyes with Proliferative Vitreoretinopathy

Research paper thumbnail of Unexpected Immunoresponse to Gal and APA Antigens in Diabetic Type 1 Patients Receiving Neonatal Pig Islets After 6 Years

Journal of Clinical Immunology, 2007

Research paper thumbnail of 861 Th1 and Th2 type cytokines regulate chemokine production from human airway epithelial cells

Journal of Allergy and Clinical Immunology, 2000

Research paper thumbnail of Cellular and mediator responses twenty-four hours after local endobronchial allergen challenge of asthmatic airways

Journal of Allergy and Clinical Immunology, 1996

The effects of acute allergen exposure on bronchoalveolar lavage cells and mediators and mucosal ... more The effects of acute allergen exposure on bronchoalveolar lavage cells and mediators and mucosal inflammatory cells were evaluated in 10 subjects with atopic asthma who underwent lavage and biopsy 24 hours after segmental endobronchial allergen challenge. Increased numbers of bronchoalveolar lavage eosinophils were retrieved from the allergen-challenged sites compared with the saline-challenged sites (mean 21.4 vs 1.5 x 10(3) cells/ml; p < 0.02). Numbers of neutrophils and proportions of CD4+, CD8+, CD25+, and HLA-DR+ T cells were similar at the saline- and allergen-challenged sites. In contrast to the bronchoalveolar lavage findings, eosinophil numbers were not increased in the bronchial submucosa or epithelium. There was also no significant difference in neutrophils, mast cells, CD3+, CD4+, or CD8+ T cells in the submucosa after allergen challenge, but the number of activated (CD25+) T lymphocytes in the mucosa did increase after allergen challenge. Allergen challenge did not induce any significant change in endothelial expression of P-selectin, E-selectin, intercellular adhesion molecule-1, or vascular cell adhesion molecule-1. CD11a+ and very late antigen-4+ cell numbers were similar in the saline- and allergen-challenged sites. This study suggests that in patients with very mild asthma, local allergen challenge induces persistent bronchoalveolar lavage eosinophilia, but the recruitment process seems to have diminished or ceased by 24 hours.

Research paper thumbnail of Cultured nasal polyps from nonatopic and atopic patients release RANTES spontaneously and after stimulation with phytohemagglutinin☆☆☆★★★

Journal of Allergy and Clinical Immunology, 1997

Eosinophil infiltration of tissue is a hallmark of nasal polyposis in both nonatopic and atopic p... more Eosinophil infiltration of tissue is a hallmark of nasal polyposis in both nonatopic and atopic patients. These cells are thought to play a key role in the nasal polyp inflammatory process. The objective of this study was to investigate whether cultured nasal polyps derived from nonatopic and atopic patients release RANTES both spontaneously and after phytohemagglutinin (PHA) stimulation. Nasal polyps were obtained from 12 subjects (6 nonatopic and 6 atopic), cut into 2 to 3 mm large specimens, and cultured for 48 hours with or without PHA. RANTES was measured in the culture supernatant by ELISA (R&D Systems, U.K.). Immunoreactive RANTES was found to be present in the culture supernatant of nasal polyps derived from both nonatopic and atopic patients with no difference between the two groups (median: 3.8 vs 2.9 pg/mg/ml). On incubation with PHA, nasal polyps from both nonatopic and atopic patients released sevenfold and 11-fold greater amounts of RANTES than unstimulated samples. As determined by immunohistochemistry, RANTES was localized to the vascular endothelium in nasal polyps from both groups of patients. This study demonstrates that cultured nasal polyps derived from both nonatopic and atopic patients release RANTES spontaneously and after PHA stimulation. This observation and the finding that RANTES is present in nasal polyp endothelial cells suggest that this chemokine may be an important mediator of eosinophil and lymphocyte recruitment in both nonatopic and atopic nasal polyposis.

Research paper thumbnail of The chemokines RANTES, MCP-3, MCP-4, eotaxin-1 and eotaxin-2 are associated with eosinophil recruitment into the airways of asthmatic children

Journal of Allergy and Clinical Immunology, 2002

The Journal of Allergy and Clinical Immunology, Volume 109, Issue 1, Pages S36, January 2002, Aut... more The Journal of Allergy and Clinical Immunology, Volume 109, Issue 1, Pages S36, January 2002, Authors:Luis M Teran; Enrique Rojas-Ramos; Ana Francisca Avalos; Lorenzo Perez-Fernandez; Francisco Cuevas. ...

Research paper thumbnail of Th2- and to a Lesser Extent Th1-Type Cytokines Upregulate the Production of both CXC (IL-8 and Gro-Alpha) and CC (RANTES, Eotaxin, Eotaxin-2, MCP-3 and MCP-4) Chemokines in Human Airway Epithelial Cells

International Archives of Allergy and Immunology, 2003

Both CXC and CC chemokines play an important role in leukocyte recruitment. However, a systematic... more Both CXC and CC chemokines play an important role in leukocyte recruitment. However, a systematic examination of their production by human airway epithelial cells (HAECs) has not been carried out. The objective of this study was to investigate whether Th1- and Th2-type cytokines regulate chemokine production in HAECs. HAECs were grown from both nasal and bronchial tissue and subsequently stimulated with either Th1- or Th2-type cytokines. Constitutive mRNA expression for gro-alpha, IL-8 and RANTES was seen in both human nasal and human bronchial epithelial cells. IL-4 was the strongest stimulus for both gene expression and protein production of the chemokines RANTES, IL-8 and gro-alpha, while both IL-13 and IFN-gamma were weaker inducers of these chemokines, with the exception of gro-alpha (IL-13 was a strong stimulus for gro-alpha production). TNF-alpha synergized with IL-4, and to a lesser extent with IFN-gamma and IL-13, to release RANTES, IL-8 and gro-alpha. IL-4 and to a lesser extent IL-13 and IFN-gamma stimulated the production of MCP-3 and -4, eotaxin and eotaxin-2 immunoreactivities. However, no induction of the mRNAs encoding these chemokines was observed, suggesting that they may be released from a preformed pool within the HAECs. These findings suggest that when released into the airways, Th2- and to a lesser extent Th1-type cytokines may stimulate recruitment of eosinophils and neutrophils through the release of CC (RANTES, MCP-3 and -4, eotaxin and eotaxin-2) and CXC chemokines (gro-alpha and IL-8).

Research paper thumbnail of RANTES Production by Cytokine–Stimulated Nasal Fibroblasts: Its Inhibition by Glucocorticoids

International Archives of Allergy and Immunology, 1998

Nasal fibroblasts play an important role in both nasal polyposis and nasal allergic diseases and ... more Nasal fibroblasts play an important role in both nasal polyposis and nasal allergic diseases and they are known to release a number of proinflammatory cytokines, including GM–CSF, IL–8 and IL–6. The aim of this present work was to investigate whether cytokine–stimulated nasal fibroblasts release biologically active RANTES as well as to study the effect of corticosteroids on the ability of

Research paper thumbnail of Granulocyte Recruitment by Human Mast Cell Tryptase

International Archives of Allergy and Immunology, 1995

... Eosinophil; Epithelial cells; Mast cell; Neutrophil; Tryptase. goto top of page Author Contac... more ... Eosinophil; Epithelial cells; Mast cell; Neutrophil; Tryptase. goto top of page Author Contacts. Correspondence to: Dr. Andrew F. Walls, University Medicine, Level F, Southampton General Hospital, Tremona Road, Southampton SO16 6YD (UK). goto top of page Article Information ...

Research paper thumbnail of Neutrophil Influx and lnterleukin-8 Release after Segmental Allergen or Saline Challenge in Asthmatics

International Archives of Allergy and Immunology, 1995

Neutrophils have been associated with the late asthmatic reaction. However, their role in this di... more Neutrophils have been associated with the late asthmatic reaction. However, their role in this disease is not well understood. In this study, we have measured neutrophil influx and release of interleukin-8 into asthmatic airways after endobronchial challenge with either allergen or saline solution.Copyright © 1995 S. Karger AG, Basel

Research paper thumbnail of Role of monocyte chemotactic protein-3 and -4 in children with virus exacerbation of asthma

European Respiratory Journal, 2008

Research paper thumbnail of Effects of 0.2 ppm ozone on biomarkers of inflammation in bronchoalveolar lavage fluid and bronchial mucosa of healthy subjects

European Respiratory Journal, 1998

Research paper thumbnail of Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma

European Respiratory Journal, 2003

Eosinophil recruitment into the airways is a feature of asthma in children. However, the mechanis... more Eosinophil recruitment into the airways is a feature of asthma in children. However, the mechanisms by which these cells migrate into the airways are not fully understood. The present study investigated the presence of the eosinophil-activating chemokines regulated on activation, normal T-cell expressed and secreted (RANTES), monocyte chemotactic proteins (MCP)-3 and -4, and eotaxins-1 and -2 in the bronchoalveolar lavage (BAL) fluid obtained from both asthmatic (n=10, age 6-10 yrs) and normal children (n=10, age 5-10 yrs). Measurements of chemokines in BAL fluid showed that levels of RANTES, MCPs-3 and -4, and eotaxins-1 and -2 were significantly increased in fluid obtained from asthmatic children when compared with normal children. Among the different chemokines, RANTES was the cytokine released in greatest quantities in BAL fluid from asthmatic children. There was a significant correlation between the concentrations of MCP-4 and eosinophil numbers in BAL fluid and a trend between both chemokines MCP-3 and eotaxin-2 and eosinophils. Interestingly, the levels of most chemokines correlated with one another. These findings suggest that RANTES monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 may regulate eosinophil trafficking into the airways of asthmatic children in a coordinated manner.

Research paper thumbnail of CC chemokine ligand 1 is released into the airways of atopic asthmatics

European Respiratory Journal, 2006