Farzaneh Moshiri | University of Ferrara (original) (raw)
Papers by Farzaneh Moshiri
A total of 800 sera collected from six herds of health and aborted cows in Golestan province in I... more A total of 800 sera collected from six herds of health and aborted cows in Golestan province in Iran (579 without abortion history, 221 with abortion history) were examined for presence of Neospora caninum antibodies by using commercial ELISA kit. The overall seroprevalence of Neospora caninum antibodies was 107 from 800 (13.37±2.36%, α=0.05). Comparison of Neospora caninum serological status in age groups (<2 years, 2-4 years, >4 years) showed no significant difference (p>0.05). The prevalence of Neospora caninum was higher in the aborted cows than in non aborted cows. This is the first study on Neospora caninum antibodies in dairy cattle herds in Golestan province in the north east of Iran.
West Indian Medical Journal, Jul 27, 2015
Objective: Human papilloma virus (HPV) can be the aetiologic factor in the benign or malignant oe... more Objective: Human papilloma virus (HPV) can be the aetiologic factor in the benign or malignant oesophageal epithelium. Human papilloma virus is suspected of causing extragenital cancers, including: cancers of the oral cavity, larynx, oesophagus and the lungs. More than 40 genotypes of HPV that infect the anogenital area are associated with a large spectrum of diseases from benign proliferation to invasive cancers. The aims of our study were to evaluate the prevalence of HPV-infection in non-cancer oesophageal lesions in Mazandran, North of Iran, and identify the prevalence of HPV in benign lesions. Methods: A total of 104 non-cancer oesophageal samples were collected in paraffinated blocks of tissue archived in pathology. After deparaffinization and DNA extraction, nested polymerase chain reaction (nPCR) method was performed by HPV L1 primer pairs (MY09/MY11 and Gp5+/GP6+). Results: Of 104 cases, 35 (33.7%) were HPV DNA positive. By histopathology, 61.5%, 31.8%, 30.4% and 30% of dysplasia, oesophagitis, normal tissue and ulcerative lesions were positive for HPV DNA, respectively. Also, the lower-third (distal) of the oesophagus had less infection of HPV DNA (18.8%) than the upper and middle-third (about 46%). The highest prevalence of HPV DNA was found in cases > 75 years old. Conclusion: To our knowledge, considering the highest rate of HPV-infection in people more than 75 years, we recommend the diagnostic procedures at a lower age. Also, the proximal part of the oesophagus is more infected with HPV than the distal part.
Medical Journal of the Islamic Republic of Iran
Due to the demographic characteristics and structure of the country's service delivery system, on... more Due to the demographic characteristics and structure of the country's service delivery system, one of the most important non-communicable disease control programs is the National Cancer Control Program, which needs to be developed in the long term with a scientific approach and cross-sectoral cooperation and stakeholder participation. →What this article adds: • The IrNCCP has 4 main components, including Prevention, Early Detection, Diagnosis and Treatment, and Supportive and Palliative care, as well as 7 supporting components including Governance and policy-making, Cancer Research, Developing facilities, equipment, and service delivery network, Providing and managing human resources, Providing and managing financial resources, Cancer information system management and registry, and Participation of NGOs, charities, and the private sector. • Strengthening the governance structure of the IrNCCP is necessary both in terms of full implementation of this program and achieving the expected goals and health outcomes, as well as in terms of evaluating the progress of this program and its improvement.
<p>CD1 nude mice (n=6) bearing Hep3B xenografts were treated intra-tumorally either with PB... more <p>CD1 nude mice (n=6) bearing Hep3B xenografts were treated intra-tumorally either with PBS or with Ad-199T (5x10<sup>8</sup> I.U. each treatment, for a total of six). (<b>A</b>) A tumor growth curve was built by measuring the size of tumors every two days. The results shown a significant difference between the PBS-treated group and the Ad-199T-treated one (p=0.001), confirming the antitumor activity of Ad-199T virus. (<b>B</b>) Kaplan-Meier survival plot showed a median survival of 24 days for untreated animals and 45 days for Ad-199T treated animals, thus indicating a longer survival time in animals treated with the oncolytic adenovirus. This difference was highly significant according to the log-rank test (p <0.0001).</p
doi: 10.3389/fgene.2013.00064 miR-221 affects multiple cancer pathways by modulating the level of... more doi: 10.3389/fgene.2013.00064 miR-221 affects multiple cancer pathways by modulating the level of hundreds messenger RNAs
<p>To assess replication properties of AD-199T <i>in </i><i>vivo</i>... more <p>To assess replication properties of AD-199T <i>in </i><i>vivo</i>, 1x10<sup>8</sup> I.U. of Ad-199T or 1x10<sup>8</sup> I.U. of Ad-Control were intra-hepatically injected into 3 days old B6D2 mice. At 72 hours after infection, livers were collected and viral DNA quantified using analytical PCR (<b>A</b>) and quantitative PCR as fold change copies of Adeno DNA referred to the lower level of Adenovirus DNA copies (<b>B</b>) The replication of Ad-199T (mice 1375, 1376) was significantly suppressed in normal liver comparing with Ad-Control (mice 1370, 1372). This evidence confirms that miR-199 could control Ad-199T replication in normal liver cells <i>in </i><i>vivo</i>.</p
Inhibiting the oncogenic mir-221 by microRNA sponge: toward
Gastroenterology and hepatology from bed to bench, 2014
We evaluated the capability of "microRNA sponges" in sequestering and inhibiting the ov... more We evaluated the capability of "microRNA sponges" in sequestering and inhibiting the over-expressed miR-221 in HCC cell lines. Advanced hepatocellular carcinoma (HCC) is a serious public health problem, with no effective cure at present. It has been demonstrated that the deregulation of microRNAs expression contributes to tumorigenesis. In HCC, miR-221 was shown to be up-regulated in more than 70% of the cases and was associated with higher tumor stage, metastasis and a shorter time to recurrence after surgery, suggesting an important pathogenic role. A tumor promoting function of miR-221 was proved in a transgenic mouse model, which was predisposed to the development of liver cancers. These findings suggested that miR-221 could represent a potential target for anti-tumor approaches. Novel adeno and adeno-associated viral vectors (AAVs) were developed: they were genetically modified to drive the expression of multiple binding sites for miR-221, the "miR-221 sponge&quo...
The down-regulation of miR-199 occurs in nearly all primary hepatocellular carcinomas (HCCs) and ... more The down-regulation of miR-199 occurs in nearly all primary hepatocellular carcinomas (HCCs) and HCC cell lines in comparison with normal liver. We exploited this miR-199 differential expression to develop a conditionally replication-competent oncolytic adenovirus, Ad-199T, and achieve tumor-specific viral expression and replication. To this aim, we introduced four copies of miR-199 target sites within the 3 ’ UTR of E1A gene, essential for viral replication. As consequence, E1A expression from Ad-199T virus was tightly regulated both at RNA and protein levels in HCC derived cell lines, and replication controlled by the level of miR-199 expression. Various approaches were used to asses in vivo properties of Ad-199T. Ad-199T replication was inhibited in normal, miR-199 positive, liver parenchyma, thus resulting in reduced hepatotoxicity. Conversely, the intrahepatic delivery of Ad-199T in newborn mice led to virus replication and fast removal of implanted HepG2 liver cancer cells. Th...
Scientific Reports
Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore,... more Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore, the need for routine COVID-19 testing in this population was evaluated. Between 1st August and 30th October 2020, 150 cancer patients were included. Symptoms of COVID-19 infection were evaluated. All eligible individuals went through RT-PCR and serological tests for COVID-19. At the same time, 920 non-cancer patients were recruited from a random sample of individuals who were subject to routine molecular and anti-body screening tests. Of 150 cancer patients, 7 (4.7%) were RT-PCR positive. Comorbidity made a significant difference in the RT-PCR positivity of cancer patients, 71.4% positive versus 25.8% negative (P-value = 0.02). The average age for negative and positive groups was 53.3 and 58.2 respectively (P-value = 0.01). No significant difference was observed between cancer and non-cancer patients regarding COVID-19 antibody tests. However, cancer patients were 3 times less likely to...
Frontiers in Genetics, 2013
Pakistan Journal of Medical Sciences, 2013
ABSTRACT
Scientific Journal of Kurdistan University of Medical Sciences
Scientific Reports
Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore,... more Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore, the need for routine COVID-19 testing in this population was evaluated. Between 1st August and 30th October 2020, 150 cancer patients were included. Symptoms of COVID-19 infection were evaluated. All eligible individuals went through RT-PCR and serological tests for COVID-19. At the same time, 920 non-cancer patients were recruited from a random sample of individuals who were subject to routine molecular and anti-body screening tests. Of 150 cancer patients, 7 (4.7%) were RT-PCR positive. Comorbidity made a significant difference in the RT-PCR positivity of cancer patients, 71.4% positive versus 25.8% negative (P-value = 0.02). The average age for negative and positive groups was 53.3 and 58.2 respectively (P-value = 0.01). No significant difference was observed between cancer and non-cancer patients regarding COVID-19 antibody tests. However, cancer patients were 3 times less likely to...
A gene encoding the mature form of the human growth hormone (hGH) was fused to the secretion sign... more A gene encoding the mature form of the human growth hormone (hGH) was fused to the secretion signal coding sequence, pelB, this hybrid gene was expressed in E. coli under the transcriptional control of bacteriophage T7\lac promote\operator system. Studying the periplasmic protein pattern of the recombinant bacteria, showed that the recombinant bacteria allowed a partially succeeded secretion of hGH into the periplasmic space. Remaining of unprocessed pelB::hGH fusion-protein was also observed in the E. coli cytoplasmic space. Comparing to IPTG induction, we have also used lactose as natural inducer for over-expression of rhGH in the present system. Our results demonstrated that lactose is as effective as IPTG for inducing expression of recombinant hGH. Introduction Variation of parameters involved in expression of recombinant protein is a phenomenon commonly observed in designing strategies for genetically engineering proteins. Different permutation and combinations of vectors, prom...
Hepatocellular carcinoma (HCC) is a serious public health problem, without an effective cure. It ... more Hepatocellular carcinoma (HCC) is a serious public health problem, without an effective cure. It has been demonstrated that the deregulation of microRNAs expression contribute to tumorigenesis. In HCC, miR-221 was shown to be up-regulated in more than 70% of the cases and was associated with higher tumor stage, metastasis and a shorter time to recurrence after surgery. A tumor promoting function of miR-221 was proved in a transgenic mouse model, which was predisposed to the development of liver cancers. These findings suggested that miR-221 could represent a potential target for anti-tumor approaches. Conversely, miR-199a is of interest because its level was shown to be reduced in almost 100% of HCC, it was significantly correlated with poor prognosis and was shown to target c-Met, an oncogene involved in invasion and metastasis. In the present thesis, novel Adenoviruses and Adeno-Associated viral vector (AAVVs) were developed. They were genetically modified to drive the expression ...
A total of 800 sera collected from six herds of health and aborted cows in Golestan province in I... more A total of 800 sera collected from six herds of health and aborted cows in Golestan province in Iran (579 without abortion history, 221 with abortion history) were examined for presence of Neospora caninum antibodies by using commercial ELISA kit. The overall seroprevalence of Neospora caninum antibodies was 107 from 800 (13.37±2.36%, α=0.05). Comparison of Neospora caninum serological status in age groups (<2 years, 2-4 years, >4 years) showed no significant difference (p>0.05). The prevalence of Neospora caninum was higher in the aborted cows than in non aborted cows. This is the first study on Neospora caninum antibodies in dairy cattle herds in Golestan province in the north east of Iran.
West Indian Medical Journal, Jul 27, 2015
Objective: Human papilloma virus (HPV) can be the aetiologic factor in the benign or malignant oe... more Objective: Human papilloma virus (HPV) can be the aetiologic factor in the benign or malignant oesophageal epithelium. Human papilloma virus is suspected of causing extragenital cancers, including: cancers of the oral cavity, larynx, oesophagus and the lungs. More than 40 genotypes of HPV that infect the anogenital area are associated with a large spectrum of diseases from benign proliferation to invasive cancers. The aims of our study were to evaluate the prevalence of HPV-infection in non-cancer oesophageal lesions in Mazandran, North of Iran, and identify the prevalence of HPV in benign lesions. Methods: A total of 104 non-cancer oesophageal samples were collected in paraffinated blocks of tissue archived in pathology. After deparaffinization and DNA extraction, nested polymerase chain reaction (nPCR) method was performed by HPV L1 primer pairs (MY09/MY11 and Gp5+/GP6+). Results: Of 104 cases, 35 (33.7%) were HPV DNA positive. By histopathology, 61.5%, 31.8%, 30.4% and 30% of dysplasia, oesophagitis, normal tissue and ulcerative lesions were positive for HPV DNA, respectively. Also, the lower-third (distal) of the oesophagus had less infection of HPV DNA (18.8%) than the upper and middle-third (about 46%). The highest prevalence of HPV DNA was found in cases > 75 years old. Conclusion: To our knowledge, considering the highest rate of HPV-infection in people more than 75 years, we recommend the diagnostic procedures at a lower age. Also, the proximal part of the oesophagus is more infected with HPV than the distal part.
Medical Journal of the Islamic Republic of Iran
Due to the demographic characteristics and structure of the country's service delivery system, on... more Due to the demographic characteristics and structure of the country's service delivery system, one of the most important non-communicable disease control programs is the National Cancer Control Program, which needs to be developed in the long term with a scientific approach and cross-sectoral cooperation and stakeholder participation. →What this article adds: • The IrNCCP has 4 main components, including Prevention, Early Detection, Diagnosis and Treatment, and Supportive and Palliative care, as well as 7 supporting components including Governance and policy-making, Cancer Research, Developing facilities, equipment, and service delivery network, Providing and managing human resources, Providing and managing financial resources, Cancer information system management and registry, and Participation of NGOs, charities, and the private sector. • Strengthening the governance structure of the IrNCCP is necessary both in terms of full implementation of this program and achieving the expected goals and health outcomes, as well as in terms of evaluating the progress of this program and its improvement.
<p>CD1 nude mice (n=6) bearing Hep3B xenografts were treated intra-tumorally either with PB... more <p>CD1 nude mice (n=6) bearing Hep3B xenografts were treated intra-tumorally either with PBS or with Ad-199T (5x10<sup>8</sup> I.U. each treatment, for a total of six). (<b>A</b>) A tumor growth curve was built by measuring the size of tumors every two days. The results shown a significant difference between the PBS-treated group and the Ad-199T-treated one (p=0.001), confirming the antitumor activity of Ad-199T virus. (<b>B</b>) Kaplan-Meier survival plot showed a median survival of 24 days for untreated animals and 45 days for Ad-199T treated animals, thus indicating a longer survival time in animals treated with the oncolytic adenovirus. This difference was highly significant according to the log-rank test (p <0.0001).</p
doi: 10.3389/fgene.2013.00064 miR-221 affects multiple cancer pathways by modulating the level of... more doi: 10.3389/fgene.2013.00064 miR-221 affects multiple cancer pathways by modulating the level of hundreds messenger RNAs
<p>To assess replication properties of AD-199T <i>in </i><i>vivo</i>... more <p>To assess replication properties of AD-199T <i>in </i><i>vivo</i>, 1x10<sup>8</sup> I.U. of Ad-199T or 1x10<sup>8</sup> I.U. of Ad-Control were intra-hepatically injected into 3 days old B6D2 mice. At 72 hours after infection, livers were collected and viral DNA quantified using analytical PCR (<b>A</b>) and quantitative PCR as fold change copies of Adeno DNA referred to the lower level of Adenovirus DNA copies (<b>B</b>) The replication of Ad-199T (mice 1375, 1376) was significantly suppressed in normal liver comparing with Ad-Control (mice 1370, 1372). This evidence confirms that miR-199 could control Ad-199T replication in normal liver cells <i>in </i><i>vivo</i>.</p
Inhibiting the oncogenic mir-221 by microRNA sponge: toward
Gastroenterology and hepatology from bed to bench, 2014
We evaluated the capability of "microRNA sponges" in sequestering and inhibiting the ov... more We evaluated the capability of "microRNA sponges" in sequestering and inhibiting the over-expressed miR-221 in HCC cell lines. Advanced hepatocellular carcinoma (HCC) is a serious public health problem, with no effective cure at present. It has been demonstrated that the deregulation of microRNAs expression contributes to tumorigenesis. In HCC, miR-221 was shown to be up-regulated in more than 70% of the cases and was associated with higher tumor stage, metastasis and a shorter time to recurrence after surgery, suggesting an important pathogenic role. A tumor promoting function of miR-221 was proved in a transgenic mouse model, which was predisposed to the development of liver cancers. These findings suggested that miR-221 could represent a potential target for anti-tumor approaches. Novel adeno and adeno-associated viral vectors (AAVs) were developed: they were genetically modified to drive the expression of multiple binding sites for miR-221, the "miR-221 sponge&quo...
The down-regulation of miR-199 occurs in nearly all primary hepatocellular carcinomas (HCCs) and ... more The down-regulation of miR-199 occurs in nearly all primary hepatocellular carcinomas (HCCs) and HCC cell lines in comparison with normal liver. We exploited this miR-199 differential expression to develop a conditionally replication-competent oncolytic adenovirus, Ad-199T, and achieve tumor-specific viral expression and replication. To this aim, we introduced four copies of miR-199 target sites within the 3 ’ UTR of E1A gene, essential for viral replication. As consequence, E1A expression from Ad-199T virus was tightly regulated both at RNA and protein levels in HCC derived cell lines, and replication controlled by the level of miR-199 expression. Various approaches were used to asses in vivo properties of Ad-199T. Ad-199T replication was inhibited in normal, miR-199 positive, liver parenchyma, thus resulting in reduced hepatotoxicity. Conversely, the intrahepatic delivery of Ad-199T in newborn mice led to virus replication and fast removal of implanted HepG2 liver cancer cells. Th...
Scientific Reports
Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore,... more Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore, the need for routine COVID-19 testing in this population was evaluated. Between 1st August and 30th October 2020, 150 cancer patients were included. Symptoms of COVID-19 infection were evaluated. All eligible individuals went through RT-PCR and serological tests for COVID-19. At the same time, 920 non-cancer patients were recruited from a random sample of individuals who were subject to routine molecular and anti-body screening tests. Of 150 cancer patients, 7 (4.7%) were RT-PCR positive. Comorbidity made a significant difference in the RT-PCR positivity of cancer patients, 71.4% positive versus 25.8% negative (P-value = 0.02). The average age for negative and positive groups was 53.3 and 58.2 respectively (P-value = 0.01). No significant difference was observed between cancer and non-cancer patients regarding COVID-19 antibody tests. However, cancer patients were 3 times less likely to...
Frontiers in Genetics, 2013
Pakistan Journal of Medical Sciences, 2013
ABSTRACT
Scientific Journal of Kurdistan University of Medical Sciences
Scientific Reports
Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore,... more Cancer patients are at risk for severe complications or death from COVID-19 infection. Therefore, the need for routine COVID-19 testing in this population was evaluated. Between 1st August and 30th October 2020, 150 cancer patients were included. Symptoms of COVID-19 infection were evaluated. All eligible individuals went through RT-PCR and serological tests for COVID-19. At the same time, 920 non-cancer patients were recruited from a random sample of individuals who were subject to routine molecular and anti-body screening tests. Of 150 cancer patients, 7 (4.7%) were RT-PCR positive. Comorbidity made a significant difference in the RT-PCR positivity of cancer patients, 71.4% positive versus 25.8% negative (P-value = 0.02). The average age for negative and positive groups was 53.3 and 58.2 respectively (P-value = 0.01). No significant difference was observed between cancer and non-cancer patients regarding COVID-19 antibody tests. However, cancer patients were 3 times less likely to...
A gene encoding the mature form of the human growth hormone (hGH) was fused to the secretion sign... more A gene encoding the mature form of the human growth hormone (hGH) was fused to the secretion signal coding sequence, pelB, this hybrid gene was expressed in E. coli under the transcriptional control of bacteriophage T7\lac promote\operator system. Studying the periplasmic protein pattern of the recombinant bacteria, showed that the recombinant bacteria allowed a partially succeeded secretion of hGH into the periplasmic space. Remaining of unprocessed pelB::hGH fusion-protein was also observed in the E. coli cytoplasmic space. Comparing to IPTG induction, we have also used lactose as natural inducer for over-expression of rhGH in the present system. Our results demonstrated that lactose is as effective as IPTG for inducing expression of recombinant hGH. Introduction Variation of parameters involved in expression of recombinant protein is a phenomenon commonly observed in designing strategies for genetically engineering proteins. Different permutation and combinations of vectors, prom...
Hepatocellular carcinoma (HCC) is a serious public health problem, without an effective cure. It ... more Hepatocellular carcinoma (HCC) is a serious public health problem, without an effective cure. It has been demonstrated that the deregulation of microRNAs expression contribute to tumorigenesis. In HCC, miR-221 was shown to be up-regulated in more than 70% of the cases and was associated with higher tumor stage, metastasis and a shorter time to recurrence after surgery. A tumor promoting function of miR-221 was proved in a transgenic mouse model, which was predisposed to the development of liver cancers. These findings suggested that miR-221 could represent a potential target for anti-tumor approaches. Conversely, miR-199a is of interest because its level was shown to be reduced in almost 100% of HCC, it was significantly correlated with poor prognosis and was shown to target c-Met, an oncogene involved in invasion and metastasis. In the present thesis, novel Adenoviruses and Adeno-Associated viral vector (AAVVs) were developed. They were genetically modified to drive the expression ...