Ricardo Simões | UNIMONTES universidade estadual de Montes Claros (original) (raw)
Papers by Ricardo Simões
Transplantation proceedings, 2010
To study whether treatment with L-arginine (ARG), a substrate of nitric oxide biosynthesis, atten... more To study whether treatment with L-arginine (ARG), a substrate of nitric oxide biosynthesis, attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rabbits treated with ARG (100 mgxkg(-1), intravenously) or saline solution (SS) prior to I (60 minutes) by occlusion of superior mesenteric artery and/or during R (120 minutes). After I or I/R, 2-cm jejunal segments were isolated and mounted in an organ bath to study of neurogenic contractions stimulated by electrical pulses or KCl using a digital recording system. Thin jejunal slices were stained (hematoxylin and eosin) for analysis by optical microscopy. Compared to the sham group, jejunal contractions were similar in I + ARG, but reduced in I + SS, I/R + SS, and I/R + ARG groups. The jejunal enteric nerves were damaged in I + SS, I/R + SS, and I/R + ARG, but not in I + ARG group, suggesting that ARG can attenuate intestinal dysfunctions due to I, but not to R.
Bookmarks Related papers MentionsView impact
Fertility and sterility, 2014
To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rat... more To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. Experimental research. University research laboratory. Thirty female adult rats. Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 μg/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. Biologic molecular study followed by immunohistochemical analysis. The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. Melatonin deprivation (pinealectomy) or administration may influence th...
Bookmarks Related papers MentionsView impact
![Research paper thumbnail of [Cardiomyocytes morphology and collagen quantification in the myocardium of female rats treated with isoflavones or estrogens]](https://attachments.academia-assets.com/44820379/thumbnails/1.jpg)
](Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2012
To evaluate the histomorphometry of cardiomyocytes and collagen present in the myocardium of rats... more To evaluate the histomorphometry of cardiomyocytes and collagen present in the myocardium of rats treated with a concentrated extract of soy or 17β-estradiol (E2). Twenty-eight rats were divided into four groups: GCtrl--estrus phase; GOvx--ovariectomized (Ovx) and receiving vehicle; GIso--Ovx and treated with soy extract (150 mg/kg per day); GE2--Ovx and treated with E2 (10 µg/kg per day). The drugs and vehicle (0.2 mL propylene glycol) were administered for 30 consecutive days after ovariectomy. On the last day the animals were anesthetized, the hearts removed, submerged in 10% formaldehyde and fragments of the ventricles underwent histological procedures, and the sections were stained with hematoxylin and eosin or picrosirius-red. Histomorphometric analysis (number and volume of nuclei and quantification of collagen) was performed under a light microscope with AxioVision Rel. 4.2 software, and collagen fibers were quantified using IMAGELAB-2000 software. Data were submitted to ANO...
Bookmarks Related papers MentionsView impact
Revista da Associação Médica Brasileira (1992)
Identification and quantitation of sulphated glycosaminoglycans (GAGs) in the uterus of female mi... more Identification and quantitation of sulphated glycosaminoglycans (GAGs) in the uterus of female mice during the estrous cycle. Four groups (n = 10 each) of virgin, 100-day old female mice were assembled according to the estrous cycle phase: proestrus, estrus, metaestrus and diestrus. Samples of the median portion of uterine horns were processed for light microscopy examination (H/E and Alcian blue + PAS). The GAGs were extracted and characterized by agarose gel electrophoresis. Data were analyzed by the unpaired Student's t-test. At light microscopy GAGs appear in all layers of the uterus, especially in the endometrium, between collagen fibers, in the basal membrane and around fibroblasts. Biochemical analyses disclosed presence of dermatan sulphate (DS), chondroitin sulphate (CS and heparan sulphate (HS) during all estral cycle phases. There was no clear electrophoretic separation between DS and CS, thus these two GAGs were considered together (DS+CS) (proestrus = 0.854 +/- 0.19...
Bookmarks Related papers MentionsView impact
![Research paper thumbnail of [Morphological aspects of the urethra in female rats after electrical stimulation of the pelvic floor]](https://attachments.academia-assets.com/44820388/thumbnails/1.jpg)
](Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2013
To evaluate the effects of electrical stimulation (ES) of the pelvic floor on the urethra of fema... more To evaluate the effects of electrical stimulation (ES) of the pelvic floor on the urethra of female rats. Forty adult rats were divided at random into four groups of ten animals each: Ctrl - without intervention; Sham - not submitted to ES, but with an electrode inserted into the vagina; Exp6 - submitted to six sessions of ES of the pelvic floor, and Exp12 - submitted to 12 sessions of ES of the pelvic floor. At the end of the experiment, all animals were anesthetized and the middle third of the urethra was removed, fixed in Bouin's fluid and processed for histomorphometric study. Sections were stained with hematoxylin and eosin for morphological and morphometric description, and others were stained with picrosirius red for the quantitation of total collagen. The thicknesses of the muscle layer and of the epithelium were determined, in 4 quadrants of the urethra, by performing 20 measurements per animal. The number of blood vessels present in the lamina propria was counted in th...
Bookmarks Related papers MentionsView impact
![Research paper thumbnail of [Histomorphologic and respiratory aspects of acute lung injury in rats induced by experimental sepsis and under pentoxifylline treatment]](https://attachments.academia-assets.com/44820380/thumbnails/1.jpg)
](Revista da Associação Médica Brasileira (1992)
Respiratory repercussion on acute lung injury in a model of induced sepsis intraperitoneally. Fif... more Respiratory repercussion on acute lung injury in a model of induced sepsis intraperitoneally. Fifteen animals taken at random were submitted to adult male Wistar rats. The rats were randomly divided into 3 groups (n=15): Group C - control group received only mechanical ventilation; Group S - rats received live Escherichia coli (E. coli) intraperitoneally (septic) and after 6 hours they were treated with normal saline infusion and ventilated with a low tidal volume. Group S+PTX - rats received live Escherichia coli intraperitoneally (septic) and after 6 hours they were treated with pentoxifylline (PTX) infusion and ventilated with a low tidal volume. All animals were ventilated during 180 minutes. We analyzed the arterial blood gases, gravimetric indices and histomorphometric analysis. Blood gases, wet to dry ratios, and total protein concentrations in the bronchoalveolar lavage were analyzed in all experimental groups. In the end of the experiment the partial pressure of oxygen was ...
Bookmarks Related papers MentionsView impact
ABSTRACT This paper focuses on the role of specifications on problem solving in the context of in... more ABSTRACT This paper focuses on the role of specifications on problem solving in the context of industrial design. Analyzing problem solving and how to improve that capability is not a trivial task. We propose to analyze and classify the different types of specifications in ...
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Reliability of polymeric materials is important not only for scientists and engineers, but also f... more Reliability of polymeric materials is important not only for scientists and engineers, but also for all the users of these materials, that is, for all of us. The users do not know about the chemistry of synthesis, processing, thermodynamics, or rheology. However, when buying ...
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Transplantation proceedings, 2010
To study whether treatment with L-arginine (ARG), a substrate of nitric oxide biosynthesis, atten... more To study whether treatment with L-arginine (ARG), a substrate of nitric oxide biosynthesis, attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rabbits treated with ARG (100 mgxkg(-1), intravenously) or saline solution (SS) prior to I (60 minutes) by occlusion of superior mesenteric artery and/or during R (120 minutes). After I or I/R, 2-cm jejunal segments were isolated and mounted in an organ bath to study of neurogenic contractions stimulated by electrical pulses or KCl using a digital recording system. Thin jejunal slices were stained (hematoxylin and eosin) for analysis by optical microscopy. Compared to the sham group, jejunal contractions were similar in I + ARG, but reduced in I + SS, I/R + SS, and I/R + ARG groups. The jejunal enteric nerves were damaged in I + SS, I/R + SS, and I/R + ARG, but not in I + ARG group, suggesting that ARG can attenuate intestinal dysfunctions due to I, but not to R.
Bookmarks Related papers MentionsView impact
Fertility and sterility, 2014
To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rat... more To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. Experimental research. University research laboratory. Thirty female adult rats. Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 μg/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. Biologic molecular study followed by immunohistochemical analysis. The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. Melatonin deprivation (pinealectomy) or administration may influence th...
Bookmarks Related papers MentionsView impact
Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2012
To evaluate the histomorphometry of cardiomyocytes and collagen present in the myocardium of rats... more To evaluate the histomorphometry of cardiomyocytes and collagen present in the myocardium of rats treated with a concentrated extract of soy or 17β-estradiol (E2). Twenty-eight rats were divided into four groups: GCtrl--estrus phase; GOvx--ovariectomized (Ovx) and receiving vehicle; GIso--Ovx and treated with soy extract (150 mg/kg per day); GE2--Ovx and treated with E2 (10 µg/kg per day). The drugs and vehicle (0.2 mL propylene glycol) were administered for 30 consecutive days after ovariectomy. On the last day the animals were anesthetized, the hearts removed, submerged in 10% formaldehyde and fragments of the ventricles underwent histological procedures, and the sections were stained with hematoxylin and eosin or picrosirius-red. Histomorphometric analysis (number and volume of nuclei and quantification of collagen) was performed under a light microscope with AxioVision Rel. 4.2 software, and collagen fibers were quantified using IMAGELAB-2000 software. Data were submitted to ANO...
Bookmarks Related papers MentionsView impact
Revista da Associação Médica Brasileira (1992)
Identification and quantitation of sulphated glycosaminoglycans (GAGs) in the uterus of female mi... more Identification and quantitation of sulphated glycosaminoglycans (GAGs) in the uterus of female mice during the estrous cycle. Four groups (n = 10 each) of virgin, 100-day old female mice were assembled according to the estrous cycle phase: proestrus, estrus, metaestrus and diestrus. Samples of the median portion of uterine horns were processed for light microscopy examination (H/E and Alcian blue + PAS). The GAGs were extracted and characterized by agarose gel electrophoresis. Data were analyzed by the unpaired Student's t-test. At light microscopy GAGs appear in all layers of the uterus, especially in the endometrium, between collagen fibers, in the basal membrane and around fibroblasts. Biochemical analyses disclosed presence of dermatan sulphate (DS), chondroitin sulphate (CS and heparan sulphate (HS) during all estral cycle phases. There was no clear electrophoretic separation between DS and CS, thus these two GAGs were considered together (DS+CS) (proestrus = 0.854 +/- 0.19...
Bookmarks Related papers MentionsView impact
Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2013
To evaluate the effects of electrical stimulation (ES) of the pelvic floor on the urethra of fema... more To evaluate the effects of electrical stimulation (ES) of the pelvic floor on the urethra of female rats. Forty adult rats were divided at random into four groups of ten animals each: Ctrl - without intervention; Sham - not submitted to ES, but with an electrode inserted into the vagina; Exp6 - submitted to six sessions of ES of the pelvic floor, and Exp12 - submitted to 12 sessions of ES of the pelvic floor. At the end of the experiment, all animals were anesthetized and the middle third of the urethra was removed, fixed in Bouin's fluid and processed for histomorphometric study. Sections were stained with hematoxylin and eosin for morphological and morphometric description, and others were stained with picrosirius red for the quantitation of total collagen. The thicknesses of the muscle layer and of the epithelium were determined, in 4 quadrants of the urethra, by performing 20 measurements per animal. The number of blood vessels present in the lamina propria was counted in th...
Bookmarks Related papers MentionsView impact
Revista da Associação Médica Brasileira (1992)
Respiratory repercussion on acute lung injury in a model of induced sepsis intraperitoneally. Fif... more Respiratory repercussion on acute lung injury in a model of induced sepsis intraperitoneally. Fifteen animals taken at random were submitted to adult male Wistar rats. The rats were randomly divided into 3 groups (n=15): Group C - control group received only mechanical ventilation; Group S - rats received live Escherichia coli (E. coli) intraperitoneally (septic) and after 6 hours they were treated with normal saline infusion and ventilated with a low tidal volume. Group S+PTX - rats received live Escherichia coli intraperitoneally (septic) and after 6 hours they were treated with pentoxifylline (PTX) infusion and ventilated with a low tidal volume. All animals were ventilated during 180 minutes. We analyzed the arterial blood gases, gravimetric indices and histomorphometric analysis. Blood gases, wet to dry ratios, and total protein concentrations in the bronchoalveolar lavage were analyzed in all experimental groups. In the end of the experiment the partial pressure of oxygen was ...
Bookmarks Related papers MentionsView impact
ABSTRACT This paper focuses on the role of specifications on problem solving in the context of in... more ABSTRACT This paper focuses on the role of specifications on problem solving in the context of industrial design. Analyzing problem solving and how to improve that capability is not a trivial task. We propose to analyze and classify the different types of specifications in ...
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Reliability of polymeric materials is important not only for scientists and engineers, but also f... more Reliability of polymeric materials is important not only for scientists and engineers, but also for all the users of these materials, that is, for all of us. The users do not know about the chemistry of synthesis, processing, thermodynamics, or rheology. However, when buying ...
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact
Bookmarks Related papers MentionsView impact