Mauro Mandrioli | Università degli studi di Modena e Reggio Emilia (original) (raw)

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Papers by Mauro Mandrioli

Research paper thumbnail of The yeast Wickerhamomyces anomalus (Pichia anomala) inhabits the midgut and reproductive system of the Asian malaria vector Anopheles stephensi

Environmental Microbiology, 2011

While symbiosis between bacteria and insects has been thoroughly investigated in the last two dec... more While symbiosis between bacteria and insects has been thoroughly investigated in the last two decades, investments on the study of yeasts associated with insects have been limited. Insect-associated yeasts are placed on different branches of the phylogenetic tree of fungi, indicating that these associations evolved independently on several occasions. Isolation of yeasts is frequently reported from insect habitats, and in some cases yeasts have been detected in the insect gut and in other organs/tissues. Here we show that the yeast Wickerhamomyces anomalus, previously known as Pichia anomala, is stably associated with the mosquito Anopheles stephensi, a main vector of malaria in Asia. Wickerhamomyces anomalus colonized pre-adult stages (larvae L(1)-L(4) and pupae) and adults of different sex and age and could be isolated in pure culture. By a combination of transmission electron microscopy and fluorescent in situ hybridization techniques, W. anomalus was shown to localize in the midgut and in both the male and female reproductive systems, suggesting multiple transmission patterns.

Research paper thumbnail of Bacterial Endosymbiont Localization in Hyalesthes obsoletus, the Insect Vector of Bois Noir in Vitis vinifera

Applied and Environmental Microbiology, 2011

Research paper thumbnail of Asaia , a versatile acetic acid bacterial symbiont, capable of cross-colonizing insects of phylogenetically distant genera and orders

Environmental Microbiology, 2009

Research paper thumbnail of Identification and molecular characterization of R1 transposable elements in the cabbage moth Mamestra brassicae

Research paper thumbnail of The structure of insect DNA methyltransferase 2 (DNMT2) DNA binding domain is responsible for the non-CpG methylation in insect genomes

Caryologia, 2004

... 1988; Yoder et al. 1997; Margot et al. 2000). ... 1_d-pse 180 EMP-----ESPS-2_d-mel 181 EMP---... more ... 1988; Yoder et al. 1997; Margot et al. 2000). ... 1_d-pse 180 EMP-----ESPS-2_d-mel 181 EMP-----GAIA-3_a-gam 186 -----EEIIT 4_x-lae 184 EFPSQHSIDPGRRRVIHCSE ...

Research paper thumbnail of Conservation of HP1 and methylated H3 histones as heterochromatic epigenetic markers in the holocentric chromosomes of the cabbage moth, Mamestra brassicae (Lepidoptera)

European Journal of Entomology, 2005

Research paper thumbnail of Chromosomal mapping reveals a dynamic organization of the histone genes in aphids (Hemiptera: Aphididae)

Entomologia, Jul 12, 2013

Research paper thumbnail of Analysis of heterochromatic epigenetic markers in the holocentric chromosomes of the aphid Acyrthosiphon pisum

Chromosome Research an International Journal on the Molecular Supramolecular and Evolutionary Aspects of Chromosome Biology, Feb 1, 2007

Research paper thumbnail of Distribution and molecular composition of heterochromatin in the holocentric chromosomes of the aphid Rhopalosiphum padi (Hemiptera: Aphididae)

Research paper thumbnail of A putative helical cytokine functioning in innate immune signalling in Drosophila melanogaster

Biochimica Et Biophysica Acta General Subjects, Jun 1, 2007

Research paper thumbnail of Molecular characterization of a defensin in the IZD-MB-0503 cell line derived from immunocytes of the insect Mamestra brassicae (Lepidoptera)

Biology of the Cell, 2003

Research paper thumbnail of Circulating phagocytes: the ancient and conserved interface between immune and neuroendocrine function

Biological reviews of the Cambridge Philosophical Society, Jan 9, 2015

Immune and neuroendocrine functions display significant overlap in highly divergent and evolution... more Immune and neuroendocrine functions display significant overlap in highly divergent and evolutionarily distant models such as molluscs, crustaceans, insects and mammals. Fundamental players in this crosstalk are professional phagocytes: macrophages in vertebrates and immunocytes in invertebrates. Although they have different developmental origins, macrophages and immunocytes possess comparable functions and differentiate under the control of evolutionarily conserved transcription factors. Macrophages and immunocytes share their pools of receptors, signalling molecules and pathways with neural cells and the neuro-endocrine system. In crustaceans, adult transdifferentiation of circulating haemocytes into neural cells has been documented recently. In light of developmental, molecular and functional evidence, we propose that the immune-neuroendocrine role of circulating phagocytes pre-dates the split of protostomian and deuterostomian superphyla and has been conserved during the evoluti...

Research paper thumbnail of Plant-mediated interspecific horizontal transmission of an intracellular symbiont in insects

Scientific reports, 2015

Intracellular reproductive manipulators, such as Candidatus Cardinium and Wolbachia are verticall... more Intracellular reproductive manipulators, such as Candidatus Cardinium and Wolbachia are vertically transmitted to progeny but rarely show co-speciation with the host. In sap-feeding insects, plant tissues have been proposed as alternative horizontal routes of interspecific transmission, but experimental evidence is limited. Here we report results from experiments that show that Cardinium is horizontally transmitted between different phloem sap-feeding insect species through plants. Quantitative PCR and in situ hybridization experiments indicated that the leafhopper Scaphoideus titanus releases Cardinium from its salivary glands during feeding on both artificial media and grapevine leaves. Successional time-course feeding experiments with S. titanus initially fed sugar solutions or small areas of grapevine leaves followed by feeding by the phytoplasma vector Macrosteles quadripunctulatus or the grapevine feeder Empoasca vitis revealed that the symbionts were transmitted to both speci...

Research paper thumbnail of Analysis of the expression pattern of the defensin gene in the lepidopteran Mamestra brassicae

Southern blotting experiments performed on Mamestra brassicae genomic DNA after digestion with me... more Southern blotting experiments performed on Mamestra brassicae genomic DNA after digestion with methylation-sensitive restriction enzymes indicated that defensin gene is methylated at CpG targets in the promoter region. However, defensin gene is actively transcribed despite the presence of methylation. Experiments performed by genome demethylation indicated that demethylated defensin gene resulted in an altered expression after bacterial induction. In particular, no

Research paper thumbnail of Identification and chromosomal localization of mariner-like elements in the cabbage moth Mamestra brassicae (Lepidoptera)

Research paper thumbnail of Molecular analysis of Salamander family: the new alpine salamander Salamandra atra aurorae

Research paper thumbnail of Analysis of the expression pattern of the defensin gene in the lepidopteran Mamestra

Research paper thumbnail of rDNA (18S-28S and 5S) colocalization and linkage between ribosomal genes and (TTAGGG)n telomeric sequence in the earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), revealed by single- and double-color FISH

Journal of Heredity

Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Ann... more Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes-5S rDNA, 18S-26S rDNA, and (TTAGGG)(n). Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S-26S rDNA as probes. Simultaneous (18S-26S)-5S and (18S-26S)-(TTAGGG)(n) FISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.

Research paper thumbnail of Walking on chromosomes

Research paper thumbnail of Analysis of insect holocentric chromosomes by atomic force microscopy

Research paper thumbnail of The yeast Wickerhamomyces anomalus (Pichia anomala) inhabits the midgut and reproductive system of the Asian malaria vector Anopheles stephensi

Environmental Microbiology, 2011

While symbiosis between bacteria and insects has been thoroughly investigated in the last two dec... more While symbiosis between bacteria and insects has been thoroughly investigated in the last two decades, investments on the study of yeasts associated with insects have been limited. Insect-associated yeasts are placed on different branches of the phylogenetic tree of fungi, indicating that these associations evolved independently on several occasions. Isolation of yeasts is frequently reported from insect habitats, and in some cases yeasts have been detected in the insect gut and in other organs/tissues. Here we show that the yeast Wickerhamomyces anomalus, previously known as Pichia anomala, is stably associated with the mosquito Anopheles stephensi, a main vector of malaria in Asia. Wickerhamomyces anomalus colonized pre-adult stages (larvae L(1)-L(4) and pupae) and adults of different sex and age and could be isolated in pure culture. By a combination of transmission electron microscopy and fluorescent in situ hybridization techniques, W. anomalus was shown to localize in the midgut and in both the male and female reproductive systems, suggesting multiple transmission patterns.

Research paper thumbnail of Bacterial Endosymbiont Localization in Hyalesthes obsoletus, the Insect Vector of Bois Noir in Vitis vinifera

Applied and Environmental Microbiology, 2011

Research paper thumbnail of Asaia , a versatile acetic acid bacterial symbiont, capable of cross-colonizing insects of phylogenetically distant genera and orders

Environmental Microbiology, 2009

Research paper thumbnail of Identification and molecular characterization of R1 transposable elements in the cabbage moth Mamestra brassicae

Research paper thumbnail of The structure of insect DNA methyltransferase 2 (DNMT2) DNA binding domain is responsible for the non-CpG methylation in insect genomes

Caryologia, 2004

... 1988; Yoder et al. 1997; Margot et al. 2000). ... 1_d-pse 180 EMP-----ESPS-2_d-mel 181 EMP---... more ... 1988; Yoder et al. 1997; Margot et al. 2000). ... 1_d-pse 180 EMP-----ESPS-2_d-mel 181 EMP-----GAIA-3_a-gam 186 -----EEIIT 4_x-lae 184 EFPSQHSIDPGRRRVIHCSE ...

Research paper thumbnail of Conservation of HP1 and methylated H3 histones as heterochromatic epigenetic markers in the holocentric chromosomes of the cabbage moth, Mamestra brassicae (Lepidoptera)

European Journal of Entomology, 2005

Research paper thumbnail of Chromosomal mapping reveals a dynamic organization of the histone genes in aphids (Hemiptera: Aphididae)

Entomologia, Jul 12, 2013

Research paper thumbnail of Analysis of heterochromatic epigenetic markers in the holocentric chromosomes of the aphid Acyrthosiphon pisum

Chromosome Research an International Journal on the Molecular Supramolecular and Evolutionary Aspects of Chromosome Biology, Feb 1, 2007

Research paper thumbnail of Distribution and molecular composition of heterochromatin in the holocentric chromosomes of the aphid Rhopalosiphum padi (Hemiptera: Aphididae)

Research paper thumbnail of A putative helical cytokine functioning in innate immune signalling in Drosophila melanogaster

Biochimica Et Biophysica Acta General Subjects, Jun 1, 2007

Research paper thumbnail of Molecular characterization of a defensin in the IZD-MB-0503 cell line derived from immunocytes of the insect Mamestra brassicae (Lepidoptera)

Biology of the Cell, 2003

Research paper thumbnail of Circulating phagocytes: the ancient and conserved interface between immune and neuroendocrine function

Biological reviews of the Cambridge Philosophical Society, Jan 9, 2015

Immune and neuroendocrine functions display significant overlap in highly divergent and evolution... more Immune and neuroendocrine functions display significant overlap in highly divergent and evolutionarily distant models such as molluscs, crustaceans, insects and mammals. Fundamental players in this crosstalk are professional phagocytes: macrophages in vertebrates and immunocytes in invertebrates. Although they have different developmental origins, macrophages and immunocytes possess comparable functions and differentiate under the control of evolutionarily conserved transcription factors. Macrophages and immunocytes share their pools of receptors, signalling molecules and pathways with neural cells and the neuro-endocrine system. In crustaceans, adult transdifferentiation of circulating haemocytes into neural cells has been documented recently. In light of developmental, molecular and functional evidence, we propose that the immune-neuroendocrine role of circulating phagocytes pre-dates the split of protostomian and deuterostomian superphyla and has been conserved during the evoluti...

Research paper thumbnail of Plant-mediated interspecific horizontal transmission of an intracellular symbiont in insects

Scientific reports, 2015

Intracellular reproductive manipulators, such as Candidatus Cardinium and Wolbachia are verticall... more Intracellular reproductive manipulators, such as Candidatus Cardinium and Wolbachia are vertically transmitted to progeny but rarely show co-speciation with the host. In sap-feeding insects, plant tissues have been proposed as alternative horizontal routes of interspecific transmission, but experimental evidence is limited. Here we report results from experiments that show that Cardinium is horizontally transmitted between different phloem sap-feeding insect species through plants. Quantitative PCR and in situ hybridization experiments indicated that the leafhopper Scaphoideus titanus releases Cardinium from its salivary glands during feeding on both artificial media and grapevine leaves. Successional time-course feeding experiments with S. titanus initially fed sugar solutions or small areas of grapevine leaves followed by feeding by the phytoplasma vector Macrosteles quadripunctulatus or the grapevine feeder Empoasca vitis revealed that the symbionts were transmitted to both speci...

Research paper thumbnail of Analysis of the expression pattern of the defensin gene in the lepidopteran Mamestra brassicae

Southern blotting experiments performed on Mamestra brassicae genomic DNA after digestion with me... more Southern blotting experiments performed on Mamestra brassicae genomic DNA after digestion with methylation-sensitive restriction enzymes indicated that defensin gene is methylated at CpG targets in the promoter region. However, defensin gene is actively transcribed despite the presence of methylation. Experiments performed by genome demethylation indicated that demethylated defensin gene resulted in an altered expression after bacterial induction. In particular, no

Research paper thumbnail of Identification and chromosomal localization of mariner-like elements in the cabbage moth Mamestra brassicae (Lepidoptera)

Research paper thumbnail of Molecular analysis of Salamander family: the new alpine salamander Salamandra atra aurorae

Research paper thumbnail of Analysis of the expression pattern of the defensin gene in the lepidopteran Mamestra

Research paper thumbnail of rDNA (18S-28S and 5S) colocalization and linkage between ribosomal genes and (TTAGGG)n telomeric sequence in the earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), revealed by single- and double-color FISH

Journal of Heredity

Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Ann... more Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes-5S rDNA, 18S-26S rDNA, and (TTAGGG)(n). Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S-26S rDNA as probes. Simultaneous (18S-26S)-5S and (18S-26S)-(TTAGGG)(n) FISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.

Research paper thumbnail of Walking on chromosomes

Research paper thumbnail of Analysis of insect holocentric chromosomes by atomic force microscopy